Restriction map for
pFN21AE6067
(Circular) (Six-base) MAPSORT of: pFN21AE6067.seq Check: 8844 from: 1 to: 5668
pFN21AE6067 5668 bp
With 182 enzymes: SgfI *
December 8, 2014 13:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3478 278
Size: 53 83 186 936 1942 2468
Fragments arranged by size:
2468 1942 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3011 1937
Size: 1074 4594
Acc65I G'GTAC_C
Cuts at: 2989 2989
Size: 5668
AclI AA'CG_TT
Cuts at: 1563 3795 4168 1563
Size: 2232 373 3063
Fragments arranged by size:
3063 2232 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2785 2817 3731 5115 5383 1632
Size: 1153 32 914 1384 268 1917
Fragments arranged by size:
1917 1384 1153 914 268 32
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5471
AflIII A'CryG_T
Cuts at: 1203 2750 4567 5544 1203
Size: 1547 1817 977 1327
Fragments arranged by size:
1817 1547 1327 977
AgeI A'CCGG_T
Cuts at: 4499 4499
Size: 5668
AhdI GACnn_n'nnGTC
Cuts at: 4397 4397
Size: 5668
AleI CACnn'nnGTG
Cuts at: 1166 2771 1166
Size: 1605 4063
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5668
AlwNI CAG_nnn'CTG
Cuts at: 2036 3411 4983 2036
Size: 1375 1572 2721
Fragments arranged by size:
2721 1572 1375
ApaLI G'TGCA_C
Cuts at: 3725 4881 3725
Size: 1156 4512
ApoI r'AATT_y
Cuts at: 1493 2977 3203 3355 5464 1493
Size: 1484 226 152 2109 1697
Fragments arranged by size:
2109 1697 1484 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4222 160
Size: 872 3190 1606
Fragments arranged by size:
3190 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5668
AvaI C'yCGr_G
Cuts at: 1870 1943 2993 1870
Size: 73 1050 4545
Fragments arranged by size:
4545 1050 73
BamHI G'GATC_C
Cuts at: 2998 3365 2998
Size: 367 5301
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2656 2989 4444 618
Size: 325 217 627 869 333 1455 1842
Fragments arranged by size:
1842 1455 869 627 333 325 217
BanII G_rGCy'C
Cuts at: 1391 2190 2987 1391
Size: 799 797 4072
Fragments arranged by size:
4072 799 797
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2659 2791 928
Size: 971 760 132 3805
Fragments arranged by size:
3805 971 760 132
BbvCI CC'TCA_GC
Cuts at: 2159 2724 2159
Size: 565 5103
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3859 3893 1903
Size: 34 1922 34 3678
Fragments arranged by size:
3678 1922 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3560 4770 3560
Size: 1210 4458
BclI T'GATC_A
Cuts at: 1548 2871 1548
Size: 1323 4345
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2648 4279 136
Size: 107 122 71 631 605 976 1631 1525
Fragments arranged by size:
1631 1525 976 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5663 5663
Size: 5668
BlpI GC'TnA_GC
Cuts at: 3085 3085
Size: 5668
Bme1580I G_kGCm'C
Cuts at: 1790 3729 4885 1790
Size: 1939 1156 2573
Fragments arranged by size:
2573 1939 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5668
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2084 3399 4357
Size: 416 53 83 399 24 667 1315 958
Cuts at: 4357 442
Size: 1753
Fragments arranged by size:
1753 1315 958 667 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5668
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2584 4328 1239
Size: 1345 1744 2579
Fragments arranged by size:
2579 1744 1345
Bpu10I CC'TnA_GC
Cuts at: 2159 2724 2159
Size: 565 5103
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3793 4658 4956 3793
Size: 865 298 4505
Fragments arranged by size:
4505 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4331 882
Size: 617 2832 2219
Fragments arranged by size:
2832 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3459 493
Size: 711 2255 2702
Fragments arranged by size:
2702 2255 711
BsaBI GATnn'nnATC
Cuts at: 5662 5662
Size: 5668
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2316 3475 3857 4492
Size: 53 83 186 936 783 1159 382 635
Cuts at: 4492 275
Size: 1451
Fragments arranged by size:
1451 1159 936 783 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4101 4499 4773 4920 5331 4101
Size: 398 274 147 411 4438
Fragments arranged by size:
4438 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5443
Fragments arranged by size:
5443 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2111 2114 1186
Size: 925 3 4740
Fragments arranged by size:
4740 925 3
BseYI C'CCAG_C
Cuts at: 2036 2328 2354 2815 4871 5367 2036
Size: 292 26 461 2056 496 2337
Fragments arranged by size:
2337 2056 496 461 292 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5095
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3118 3879 4907 1540
Size: 275 186 1117 761 1028 2301
Fragments arranged by size:
2301 1117 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2121 3729 3814 4885 2121
Size: 1608 85 1071 2904
Fragments arranged by size:
2904 1608 1071 85
BsmI GAATG_Cn'
Cuts at: 3179 3272 3179
Size: 93 5575
BsmBI CGTCTCn'nnnn_
Cuts at: 2288 2288
Size: 5668
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2121 2190 2987 3729 3814 4885 1391
Size: 399 331 69 797 742 85 1071 2174
Fragments arranged by size:
2174 1071 797 742 399 331 85 69
BspEI T'CCGG_A
Cuts at: 5331 5331
Size: 5668
BspHI T'CATG_A
Cuts at: 2862 3557 5287 2862
Size: 695 1730 3243
Fragments arranged by size:
3243 1730 695
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3023 844
Size: 2179 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2718 3555 1394
Size: 429 895 837 3507
Fragments arranged by size:
3507 895 837 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2277 4163 4337 65
Size: 1183 1029 1886 174 1396
Fragments arranged by size:
1886 1396 1183 1029 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4312 4499 1700
Size: 110 2502 187 2869
Fragments arranged by size:
2869 2502 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5668
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5668
BssSI C'ACGA_G
Cuts at: 3728 4740 3728
Size: 1012 4656
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5668
BstBI TT'CG_AA
Cuts at: 4472 4472
Size: 5668
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5668
BstYI r'GATC_y
Cuts at: 1972 2998 3365 3750 3767 5219 5305 5317 5663
Size: 1026 367 385 17 1452 86 12 346
Cuts at: 5663 1972
Size: 1977
Fragments arranged by size:
1977 1452 1026 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2382 513
Size: 552 1317 3799
Fragments arranged by size:
3799 1317 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4929
Fragments arranged by size:
4929 683 56
BtsI GCAGTG_nn'
Cuts at: 3184 3978 3998 3184
Size: 794 20 4854
Fragments arranged by size:
4854 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5668
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2518 2553 529
Size: 35 1954 35 3644
Fragments arranged by size:
3644 1954 35 35
DraI TTT'AAA
Cuts at: 2972 2972
Size: 5668
DraIII CAC_nnn'GTG
Cuts at: 2512 2512
Size: 5668
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4675 809
Size: 507 3359 1802
Fragments arranged by size:
3359 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2403 2514 3115
Size: 54 1425 50 275 18 573 111 601
Cuts at: 3115 4004 5633 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 601 573 275 111 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3115 1537
Size: 275 1303 4090
Fragments arranged by size:
4090 1303 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2088 2339 2588 3598 1353
Size: 735 251 249 1010 3423
Fragments arranged by size:
3423 1010 735 251 249
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4255 4629 4775 387
Size: 1064 138 2666 374 146 1280
Fragments arranged by size:
2666 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2584 2785 2817 3731 4328 5115 5383
Size: 393 952 201 32 914 597 787 268
Cuts at: 5383 1239
Size: 1524
Fragments arranged by size:
1524 952 914 787 597 393 268 201
32
EcoRI G'AATT_C
Cuts at: 2977 3355 2977
Size: 378 5290
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2758 2758
Size: 5668
FspI TGC'GCA
Cuts at: 4174 4174
Size: 5668
HaeII r_GCGC'y
Cuts at: 1678 2931 4815 1678
Size: 1253 1884 2531
Fragments arranged by size:
2531 1884 1253
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4309 4341 4383 4415 1373
Size: 32 135 32 2737 32 42 32 2626
Fragments arranged by size:
2737 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3012 3258 669
Size: 988 281 1074 246 3079
Fragments arranged by size:
3079 1074 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5668
HpaI GTT'AAC
Cuts at: 3258 3258
Size: 5668
KpnI G_GTAC'C
Cuts at: 2993 2993
Size: 5668
MfeI C'AATT_G
Cuts at: 2145 3267 2145
Size: 1122 4546
MluI A'CGCG_T
Cuts at: 5544 5544
Size: 5668
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2224 4782 4966 1305
Size: 919 2558 184 2007
Fragments arranged by size:
2558 2007 919 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2405 2516 10
Size: 54 1425 343 573 111 3162
Fragments arranged by size:
3162 1425 573 343 111 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2435 2651 2675 2771 2867 3626 3985
Size: 648 1269 216 24 96 96 759 359
Cuts at: 3985 4144 518
Size: 159 2042
Fragments arranged by size:
2042 1269 759 648 359 216 159 96
96 24
MspA1I CmG'CkG
Cuts at: 1606 2054 3084 3761 4909 5154 1606
Size: 448 1030 677 1148 245 2120
Fragments arranged by size:
2120 1148 1030 677 448 245
NcoI C'CATG_G
Cuts at: 513 1065 2382 513
Size: 552 1317 3799
Fragments arranged by size:
3799 1317 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5668
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5668
NotI GC'GGCC_GC
Cuts at: 3115 3115
Size: 5668
NspI r_CATG'y
Cuts at: 1132 1229 2754 3026 4571 1132
Size: 97 1525 272 1545 2229
Fragments arranged by size:
2229 1545 1525 272 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5668
PciI A'CATG_T
Cuts at: 2750 4567 2750
Size: 1817 3851
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2036 2350 2512 1108
Size: 928 314 162 4264
Fragments arranged by size:
4264 928 314 162
PmeI GTTT'AAAC
Cuts at: 2972 2972
Size: 5668
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2803 2835 3737 3769 5250 5282 2803
Size: 32 902 32 1481 32 3189
Fragments arranged by size:
3189 1481 902 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2795 1613
Size: 1182 4486
PsiI TTA'TAA
Cuts at: 3238 3238
Size: 5668
PstI C_TGCA'G
Cuts at: 830 3020 830
Size: 2190 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5668
SalI G'TCGA_C
Cuts at: 1936 3010 1936
Size: 1074 4594
SapI GCTCTTCn'nnn_
Cuts at: 2339 2588 2339
Size: 249 5419
SbfI CC_TGCA'GG
Cuts at: 3020 3020
Size: 5668
ScaI AGT'ACT
Cuts at: 3916 5628 3916
Size: 1712 3956
SfcI C'TryA_G
Cuts at: 826 1045 2425 2899 3016 4151 4832 5023 826
Size: 219 1380 474 117 1135 681 191 1471
Fragments arranged by size:
1471 1380 1135 681 474 219 191 117
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5668
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5668
SmaI CCC'GGG
Cuts at: 1872 2995 1872
Size: 1123 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3772 4673 4935 820
Size: 197 926 1829 901 262 1553
Fragments arranged by size:
1829 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3459 493
Size: 2966 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5668
SphI G_CATG'C
Cuts at: 1132 3026 1132
Size: 1894 3774
SspI AAT'ATT
Cuts at: 5 52 3592 5
Size: 47 3540 2081
Fragments arranged by size:
3540 2081 47
StuI AGG'CCT
Cuts at: 1517 2741 1517
Size: 1224 4444
StyI C'CwwG_G
Cuts at: 513 1065 2382 2661 3107 513
Size: 552 1317 279 446 3074
Fragments arranged by size:
3074 1317 552 446 279
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2409 2621 3695 3712 3865 4050 4495
Size: 1255 829 212 1074 17 153 185 445
Cuts at: 4495 325
Size: 1498
Fragments arranged by size:
1498 1255 1074 829 445 212 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2208 3914 5626
Size: 274 80 33 51 1443 231 1706 1712
Cuts at: 5626 96
Size: 138
Fragments arranged by size:
1712 1706 1443 274 231 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2568 2600 5628 5660 2568
Size: 32 3028 32 2576
Fragments arranged by size:
3028 2576 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5668
XbaI T'CTAG_A
Cuts at: 3004 3004
Size: 5668
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2351 2351
Size: 5668
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5668
XmaI C'CCGG_G
Cuts at: 1870 2993 1870
Size: 1123 4545
XmnI GAAnn'nnTTC
Cuts at: 2741 3797 2741
Size: 1056 4612
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3476 276
Size: 53 83 186 936 1942 2468
Fragments arranged by size:
2468 1942 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI