Restriction map for
pFN21AE6065
(Circular) (Six-base) MAPSORT of: pFN21AE6065.seq Check: 1461 from: 1 to: 5665
pFN21AE6065 5665 bp
With 182 enzymes: SgfI *
December 8, 2014 13:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3475 278
Size: 53 83 186 936 1939 2468
Fragments arranged by size:
2468 1939 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2574 3008 1937
Size: 637 434 4594
Fragments arranged by size:
4594 637 434
Acc65I G'GTAC_C
Cuts at: 2986 2986
Size: 5665
AclI AA'CG_TT
Cuts at: 1563 3792 4165 1563
Size: 2229 373 3063
Fragments arranged by size:
3063 2229 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2927 3728 5112 5380 1632
Size: 1295 801 1384 268 1917
Fragments arranged by size:
1917 1384 1295 801 268
AflII C'TTAA_G
Cuts at: 820 1017 2878 820
Size: 197 1861 3607
Fragments arranged by size:
3607 1861 197
AflIII A'CryG_T
Cuts at: 1203 2316 2507 2775 4564 5541 1203
Size: 1113 191 268 1789 977 1327
Fragments arranged by size:
1789 1327 1113 977 268 191
AgeI A'CCGG_T
Cuts at: 4496 4496
Size: 5665
AhdI GACnn_n'nnGTC
Cuts at: 4394 4394
Size: 5665
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5665
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5665
AlwNI CAG_nnn'CTG
Cuts at: 2248 3408 4980 2248
Size: 1160 1572 2933
Fragments arranged by size:
2933 1572 1160
ApaLI G'TGCA_C
Cuts at: 3722 4878 3722
Size: 1156 4509
ApoI r'AATT_y
Cuts at: 1493 2197 2497 2974 3200 3352 5461 1493
Size: 704 300 477 226 152 2109 1697
Fragments arranged by size:
2109 1697 704 477 300 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4219 160
Size: 872 3187 1606
Fragments arranged by size:
3187 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5665
AvaI C'yCGr_G
Cuts at: 1870 1943 2990 1870
Size: 73 1047 4545
Fragments arranged by size:
4545 1047 73
BamHI G'GATC_C
Cuts at: 2995 3362 2995
Size: 367 5298
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2986 4441 618
Size: 325 217 627 1199 1455 1842
Fragments arranged by size:
1842 1455 1199 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2330 2984 1391
Size: 939 654 4072
Fragments arranged by size:
4072 939 654
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4694
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3856 3890 1903
Size: 34 1919 34 3678
Fragments arranged by size:
3678 1919 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3557 4767 3557
Size: 1210 4455
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5665
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4276 136
Size: 107 122 71 631 605 2604 1525
Fragments arranged by size:
2604 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2212 5660 2212
Size: 3448 2217
BlpI GC'TnA_GC
Cuts at: 3082 3082
Size: 5665
Bme1580I G_kGCm'C
Cuts at: 1790 3726 4882 1790
Size: 1936 1156 2573
Fragments arranged by size:
2573 1936 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5665
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3396 4354 442
Size: 416 53 83 399 24 1979 958 1753
Fragments arranged by size:
1979 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5665
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2470 4325 1239
Size: 1231 1855 2579
Fragments arranged by size:
2579 1855 1231
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3790 4655 4953 3790
Size: 865 298 4502
Fragments arranged by size:
4502 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4328 882
Size: 617 2829 2219
Fragments arranged by size:
2829 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3456 493
Size: 711 2252 2702
Fragments arranged by size:
2702 2252 711
BsaBI GATnn'nnATC
Cuts at: 2438 5659 2438
Size: 3221 2444
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3472 3854 4489 275
Size: 53 83 186 936 1939 382 635 1451
Fragments arranged by size:
1939 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4098 4496 4770 4917 5328 4098
Size: 398 274 147 411 4435
Fragments arranged by size:
4435 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2443 2473 1178
Size: 30 165 30 1040 30 4370
Fragments arranged by size:
4370 1040 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5665
BseYI C'CCAG_C
Cuts at: 2163 4868 5364 2163
Size: 2705 496 2464
Fragments arranged by size:
2705 2464 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5092
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3115 3876 4904 1540
Size: 275 186 1114 761 1028 2301
Fragments arranged by size:
2301 1114 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3726 3811 4882 3726
Size: 85 1071 4509
Fragments arranged by size:
4509 1071 85
BsmI GAATG_Cn'
Cuts at: 3176 3269 3176
Size: 93 5572
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2330 2984 3726 3811 4882 1391
Size: 399 540 654 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 654 540 399 85
BspEI T'CCGG_A
Cuts at: 5328 5328
Size: 5665
BspHI T'CATG_A
Cuts at: 2409 2442 3554 5284 2409
Size: 33 1112 1730 2790
Fragments arranged by size:
2790 1730 1112 33
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2790 3020 844
Size: 1946 230 3489
Fragments arranged by size:
3489 1946 230
BsrBI CCG'CTC
Cuts at: 1394 1823 2866 3552 1394
Size: 429 1043 686 3507
Fragments arranged by size:
3507 1043 686 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2664 2721 4160 4334 65
Size: 1183 1416 57 1439 174 1396
Fragments arranged by size:
1439 1416 1396 1183 174 57
BsrFI r'CCGG_y
Cuts at: 1700 1810 4309 4496 1700
Size: 110 2499 187 2869
Fragments arranged by size:
2869 2499 187 110
BsrGI T'GTAC_A
Cuts at: 96 2483 96
Size: 2387 3278
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5665
BssSI C'ACGA_G
Cuts at: 3725 4737 3725
Size: 1012 4653
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5665
BstBI TT'CG_AA
Cuts at: 4469 4469
Size: 5665
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5665
BstYI r'GATC_y
Cuts at: 1972 2212 2729 2995 3362 3747 3764 5216 5302
Size: 240 517 266 367 385 17 1452 86
Cuts at: 5302 5314 5660 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 517 385 367 346 266 240
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4174
Fragments arranged by size:
4174 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4926
Fragments arranged by size:
4926 683 56
BtsI GCAGTG_nn'
Cuts at: 3181 3975 3995 3181
Size: 794 20 4851
Fragments arranged by size:
4851 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5665
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2569 2604 529
Size: 35 2005 35 3590
Fragments arranged by size:
3590 2005 35 35
DraI TTT'AAA
Cuts at: 2969 2969
Size: 5665
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4672 809
Size: 507 3356 1802
Fragments arranged by size:
3356 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2463 3112 4001
Size: 54 1425 50 275 18 633 649 889
Cuts at: 4001 5630 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 649 633 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3112 1537
Size: 275 1300 4090
Fragments arranged by size:
4090 1300 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3595 1353
Size: 2242 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4252 4626 4772 387
Size: 1064 138 2663 374 146 1280
Fragments arranged by size:
2663 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2470 2927 3728 4325 5112 5380 1239
Size: 393 838 457 801 597 787 268 1524
Fragments arranged by size:
1524 838 801 787 597 457 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2264 2264
Size: 5665
EcoRI G'AATT_C
Cuts at: 2974 3352 2974
Size: 378 5287
FspI TGC'GCA
Cuts at: 4171 4171
Size: 5665
HaeII r_GCGC'y
Cuts at: 1678 4812 1678
Size: 3134 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4306 4338 4380 4412 1373
Size: 32 135 32 2734 32 42 32 2626
Fragments arranged by size:
2734 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2254 3009 3255 669
Size: 988 281 316 755 246 3079
Fragments arranged by size:
3079 988 755 316 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5665
HpaI GTT'AAC
Cuts at: 3255 3255
Size: 5665
KpnI G_GTAC'C
Cuts at: 2990 2990
Size: 5665
MfeI C'AATT_G
Cuts at: 3264 3264
Size: 5665
MluI A'CGCG_T
Cuts at: 5541 5541
Size: 5665
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2326 4779 4963 1305
Size: 1021 2453 184 2007
Fragments arranged by size:
2453 2007 1021 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2465 10
Size: 54 1425 343 633 3210
Fragments arranged by size:
3210 1425 633 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2422 3623 3982 4141 518
Size: 648 1256 1201 359 159 2042
Fragments arranged by size:
2042 1256 1201 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3081 3758 4906 5151 1606
Size: 1475 677 1148 245 2120
Fragments arranged by size:
2120 1475 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4174
Fragments arranged by size:
4174 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5665
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5665
NotI GC'GGCC_GC
Cuts at: 3112 3112
Size: 5665
NspI r_CATG'y
Cuts at: 1132 1229 2320 2511 2779 3023 4568 1132
Size: 97 1091 191 268 244 1545 2229
Fragments arranged by size:
2229 1545 1091 268 244 191 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5665
PciI A'CATG_T
Cuts at: 2316 2507 2775 4564 2316
Size: 191 268 1789 3417
Fragments arranged by size:
3417 1789 268 191
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5665
PfoI T'CCnGG_A
Cuts at: 2154 2154
Size: 5665
PmeI GTTT'AAAC
Cuts at: 2969 2969
Size: 5665
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3734 3766 5247 5279 3734
Size: 32 1481 32 4120
Fragments arranged by size:
4120 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2264 2264
Size: 5665
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5665
PsiI TTA'TAA
Cuts at: 2618 3235 2618
Size: 617 5048
PstI C_TGCA'G
Cuts at: 830 3017 830
Size: 2187 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5665
SalI G'TCGA_C
Cuts at: 1936 3007 1936
Size: 1071 4594
SbfI CC_TGCA'GG
Cuts at: 3017 3017
Size: 5665
ScaI AGT'ACT
Cuts at: 3913 5625 3913
Size: 1712 3953
SexAI A'CCwGG_T
Cuts at: 2788 2788
Size: 5665
SfcI C'TryA_G
Cuts at: 826 1045 2472 2742 3013 4148 4829 5020 826
Size: 219 1427 270 271 1135 681 191 1471
Fragments arranged by size:
1471 1427 1135 681 271 270 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5665
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5665
SmaI CCC'GGG
Cuts at: 1872 2992 1872
Size: 1120 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2878 3769 4670 4932 820
Size: 197 926 935 891 901 262 1553
Fragments arranged by size:
1553 935 926 901 891 262 197
SnaBI TAC'GTA
Cuts at: 493 3456 493
Size: 2963 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5665
SphI G_CATG'C
Cuts at: 1132 3023 1132
Size: 1891 3774
SspI AAT'ATT
Cuts at: 5 52 3589 5
Size: 47 3537 2081
Fragments arranged by size:
3537 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5665
StyI C'CwwG_G
Cuts at: 513 1065 2004 3104 513
Size: 552 939 1100 3074
Fragments arranged by size:
3074 1100 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3692 3709 3862 4047 4492 325
Size: 1255 2112 17 153 185 445 1498
Fragments arranged by size:
2112 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 2483 3911
Size: 274 80 33 51 1443 204 302 1428
Cuts at: 3911 5623 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1428 302 274 204 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2622 2654 5625 5657 2622
Size: 32 2971 32 2630
Fragments arranged by size:
2971 2630 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5665
XbaI T'CTAG_A
Cuts at: 3001 3001
Size: 5665
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2284 2284
Size: 5665
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5665
XmaI C'CCGG_G
Cuts at: 1870 2990 1870
Size: 1120 4545
XmnI GAAnn'nnTTC
Cuts at: 3794 3794
Size: 5665
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3473 276
Size: 53 83 186 936 1939 2468
Fragments arranged by size:
2468 1939 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SfiI SfoI SrfI SwaI