Restriction map for
pFN21AE6062
(Circular) (Six-base) MAPSORT of: pFN21AE6062.seq Check: 8802 from: 1 to: 5665
pFN21AE6062 5665 bp
With 182 enzymes: SgfI *
December 8, 2014 13:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2797 2890 2797
Size: 93 5572
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3475 278
Size: 53 83 186 936 1939 2468
Fragments arranged by size:
2468 1939 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3008 1937
Size: 1071 4594
Acc65I G'GTAC_C
Cuts at: 2986 2986
Size: 5665
AclI AA'CG_TT
Cuts at: 1563 3792 4165 1563
Size: 2229 373 3063
Fragments arranged by size:
3063 2229 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3728 5112 5380 1632
Size: 2096 1384 268 1917
Fragments arranged by size:
2096 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5468
AflIII A'CryG_T
Cuts at: 1203 4564 5541 1203
Size: 3361 977 1327
Fragments arranged by size:
3361 1327 977
AgeI A'CCGG_T
Cuts at: 4496 4496
Size: 5665
AhdI GACnn_n'nnGTC
Cuts at: 2828 4394 2828
Size: 1566 4099
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5665
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2513 1184
Size: 1329 4336
AlwNI CAG_nnn'CTG
Cuts at: 3408 4980 3408
Size: 1572 4093
ApaLI G'TGCA_C
Cuts at: 3722 4878 3722
Size: 1156 4509
ApoI r'AATT_y
Cuts at: 1493 2112 2974 3200 3352 5461 1493
Size: 619 862 226 152 2109 1697
Fragments arranged by size:
2109 1697 862 619 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4219 160
Size: 872 3187 1606
Fragments arranged by size:
3187 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5665
AvaI C'yCGr_G
Cuts at: 1870 1943 2990 1870
Size: 73 1047 4545
Fragments arranged by size:
4545 1047 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2949 2982 2949
Size: 33 5632
BamHI G'GATC_C
Cuts at: 2076 2995 3362 2076
Size: 919 367 4379
Fragments arranged by size:
4379 919 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2986 4441 618
Size: 325 217 627 1199 1455 1842
Fragments arranged by size:
1842 1455 1199 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2296 2984 1391
Size: 905 688 4072
Fragments arranged by size:
4072 905 688
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2183 2918 928
Size: 971 284 735 3675
Fragments arranged by size:
3675 971 735 284
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2469 2503 3856 3890 1903
Size: 34 50 34 448 34 1353 34 3678
Fragments arranged by size:
3678 1353 448 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3557 4767 3557
Size: 1210 4455
BclI T'GATC_A
Cuts at: 1548 2025 1548
Size: 477 5188
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4276 136
Size: 107 122 71 631 605 2604 1525
Fragments arranged by size:
2604 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5660 5660
Size: 5665
BlpI GC'TnA_GC
Cuts at: 3082 3082
Size: 5665
Bme1580I G_kGCm'C
Cuts at: 1790 3726 4882 1790
Size: 1936 1156 2573
Fragments arranged by size:
2573 1936 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5665
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3396 4354 442
Size: 416 53 83 399 24 1979 958 1753
Fragments arranged by size:
1979 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5665
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2108 4325 1239
Size: 869 2217 2579
Fragments arranged by size:
2579 2217 869
Bpu10I CC'TnA_GC
Cuts at: 2039 2039
Size: 5665
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2437 3790 4655 4953 2437
Size: 1353 865 298 3149
Fragments arranged by size:
3149 1353 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4328 882
Size: 617 2829 2219
Fragments arranged by size:
2829 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3456 493
Size: 711 2252 2702
Fragments arranged by size:
2702 2252 711
BsaBI GATnn'nnATC
Cuts at: 5659 5659
Size: 5665
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3472 3854 4489 275
Size: 53 83 186 936 1939 382 635 1451
Fragments arranged by size:
1939 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4098 4496 4770 4917 5328 4098
Size: 398 274 147 411 4435
Fragments arranged by size:
4435 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5440
Fragments arranged by size:
5440 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5665
BseYI C'CCAG_C
Cuts at: 2583 4868 5364 2583
Size: 2285 496 2884
Fragments arranged by size:
2884 2285 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2868 1163
Size: 573 1132 3960
Fragments arranged by size:
3960 1132 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3115 3876 4904 1540
Size: 275 186 1114 761 1028 2301
Fragments arranged by size:
2301 1114 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3726 3811 4882 3726
Size: 85 1071 4509
Fragments arranged by size:
4509 1071 85
BsmI GAATG_Cn'
Cuts at: 3176 3269 3176
Size: 93 5572
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2296 2984 3726 3811 4882 1391
Size: 399 506 688 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 688 506 399 85
BspEI T'CCGG_A
Cuts at: 5328 5328
Size: 5665
BspHI T'CATG_A
Cuts at: 2194 2640 3554 5284 2194
Size: 446 914 1730 2575
Fragments arranged by size:
2575 1730 914 446
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2797 2890 3020 844
Size: 1953 93 130 3489
Fragments arranged by size:
3489 1953 130 93
BsrBI CCG'CTC
Cuts at: 1394 1823 3552 1394
Size: 429 1729 3507
Fragments arranged by size:
3507 1729 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2667 4160 4334 65
Size: 1183 1419 1493 174 1396
Fragments arranged by size:
1493 1419 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4309 4496 1700
Size: 110 2499 187 2869
Fragments arranged by size:
2869 2499 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5665
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5665
BssSI C'ACGA_G
Cuts at: 3725 4737 3725
Size: 1012 4653
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5665
BstBI TT'CG_AA
Cuts at: 2479 4469 2479
Size: 1990 3675
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5665
BstXI CCAn_nnnn'nTGG
Cuts at: 2374 2819 2374
Size: 445 5220
BstYI r'GATC_y
Cuts at: 1972 2076 2995 3362 3747 3764 5216 5302 5314
Size: 104 919 367 385 17 1452 86 12
Cuts at: 5314 5660 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 919 385 367 346 104 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2379 2895 513
Size: 552 1314 516 3283
Fragments arranged by size:
3283 1314 552 516
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2798 487
Size: 683 56 1572 3354
Fragments arranged by size:
3354 1572 683 56
BtsI GCAGTG_nn'
Cuts at: 3181 3975 3995 3181
Size: 794 20 4851
Fragments arranged by size:
4851 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5665
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5630
DraI TTT'AAA
Cuts at: 2969 2969
Size: 5665
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4672 809
Size: 507 3356 1802
Fragments arranged by size:
3356 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2226 2376 3112
Size: 54 1425 50 275 18 396 150 736
Cuts at: 3112 4001 5630 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 736 396 275 150 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3112 1537
Size: 275 1300 4090
Fragments arranged by size:
4090 1300 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3595 1353
Size: 2242 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4252 4626 4772 387
Size: 1064 138 2663 374 146 1280
Fragments arranged by size:
2663 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2108 3728 4325 5112 5380 1239
Size: 393 476 1620 597 787 268 1524
Fragments arranged by size:
1620 1524 787 597 476 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2057 2057
Size: 5665
EcoRI G'AATT_C
Cuts at: 2112 2974 3352 2112
Size: 862 378 4425
Fragments arranged by size:
4425 862 378
EcoRV GAT'ATC
Cuts at: 2602 2602
Size: 5665
FspI TGC'GCA
Cuts at: 4171 4171
Size: 5665
HaeII r_GCGC'y
Cuts at: 1678 4812 1678
Size: 3134 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2814 2846 4306 4338 4380
Size: 32 135 32 1242 32 1460 32 42
Cuts at: 4380 4412 1373
Size: 32 2626
Fragments arranged by size:
2626 1460 1242 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3009 3255 669
Size: 988 281 1071 246 3079
Fragments arranged by size:
3079 1071 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2482 748
Size: 1734 3931
HpaI GTT'AAC
Cuts at: 3255 3255
Size: 5665
KpnI G_GTAC'C
Cuts at: 2990 2990
Size: 5665
MfeI C'AATT_G
Cuts at: 3264 3264
Size: 5665
MluI A'CGCG_T
Cuts at: 5541 5541
Size: 5665
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4779 4963 1305
Size: 3474 184 2007
Fragments arranged by size:
3474 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2228 2378 10
Size: 54 1425 343 396 150 3297
Fragments arranged by size:
3297 1425 396 343 150 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2177 2372 2574 2763 2774 3623 3982
Size: 648 1011 195 202 189 11 849 359
Cuts at: 3982 4141 518
Size: 159 2042
Fragments arranged by size:
2042 1011 849 648 359 202 195 189
159 11
MspA1I CmG'CkG
Cuts at: 1606 2255 3081 3758 4906 5151 1606
Size: 649 826 677 1148 245 2120
Fragments arranged by size:
2120 1148 826 677 649 245
NcoI C'CATG_G
Cuts at: 513 1065 2379 2895 513
Size: 552 1314 516 3283
Fragments arranged by size:
3283 1314 552 516
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5665
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5665
NotI GC'GGCC_GC
Cuts at: 3112 3112
Size: 5665
NruI TCG'CGA
Cuts at: 2298 2298
Size: 5665
NspI r_CATG'y
Cuts at: 1132 1229 2523 3023 4568 1132
Size: 97 1294 500 1545 2229
Fragments arranged by size:
2229 1545 1294 500 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5665
PciI A'CATG_T
Cuts at: 4564 4564
Size: 5665
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5665
PfoI T'CCnGG_A
Cuts at: 2053 2355 2053
Size: 302 5363
PmeI GTTT'AAAC
Cuts at: 2969 2969
Size: 5665
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3734 3766 5247 5279 3734
Size: 32 1481 32 4120
Fragments arranged by size:
4120 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2057 2057
Size: 5665
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5665
PsiI TTA'TAA
Cuts at: 3235 3235
Size: 5665
PstI C_TGCA'G
Cuts at: 830 2537 3017 830
Size: 1707 480 3478
Fragments arranged by size:
3478 1707 480
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5665
PvuII CAG'CTG
Cuts at: 2255 2255
Size: 5665
SalI G'TCGA_C
Cuts at: 1936 3007 1936
Size: 1071 4594
SbfI CC_TGCA'GG
Cuts at: 3017 3017
Size: 5665
ScaI AGT'ACT
Cuts at: 3913 5625 3913
Size: 1712 3953
SfcI C'TryA_G
Cuts at: 826 1045 2533 3013 4148 4829 5020 826
Size: 219 1488 480 1135 681 191 1471
Fragments arranged by size:
1488 1471 1135 681 480 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5665
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5665
SmaI CCC'GGG
Cuts at: 1872 2992 1872
Size: 1120 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2416 3769 4670 4932 820
Size: 197 926 473 1353 901 262 1553
Fragments arranged by size:
1553 1353 926 901 473 262 197
SnaBI TAC'GTA
Cuts at: 493 3456 493
Size: 2963 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5665
SphI G_CATG'C
Cuts at: 1132 3023 1132
Size: 1891 3774
SspI AAT'ATT
Cuts at: 5 52 3589 5
Size: 47 3537 2081
Fragments arranged by size:
3537 2081 47
StuI AGG'CCT
Cuts at: 1517 2085 1517
Size: 568 5097
StyI C'CwwG_G
Cuts at: 513 1065 2379 2895 3104 513
Size: 552 1314 516 209 3074
Fragments arranged by size:
3074 1314 552 516 209
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3692 3709 3862 4047 4492 325
Size: 1255 2112 17 153 185 445 1498
Fragments arranged by size:
2112 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3911 5623 96
Size: 274 80 33 51 1443 1934 1712 138
Fragments arranged by size:
1934 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5625 5657 5625
Size: 32 5633
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5665
XbaI T'CTAG_A
Cuts at: 3001 3001
Size: 5665
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2105 2105
Size: 5665
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5665
XmaI C'CCGG_G
Cuts at: 1870 2990 1870
Size: 1120 4545
XmnI GAAnn'nnTTC
Cuts at: 3794 3794
Size: 5665
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3473 276
Size: 53 83 186 936 1939 2468
Fragments arranged by size:
2468 1939 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NruI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
FalI FseI FspAI KasI NaeI NarI NgoMIV NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI