Restriction map for
pFN21AE6060
(Circular) (Six-base) MAPSORT of: pFN21AE6060.seq Check: 9279 from: 1 to: 5665
pFN21AE6060 5665 bp
With 182 enzymes: SgfI *
December 8, 2014 13:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2726 2726
Size: 5665
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3475 278
Size: 53 83 186 936 1939 2468
Fragments arranged by size:
2468 1939 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3008 1937
Size: 1071 4594
Acc65I G'GTAC_C
Cuts at: 2986 2986
Size: 5665
AclI AA'CG_TT
Cuts at: 1563 3792 4165 1563
Size: 2229 373 3063
Fragments arranged by size:
3063 2229 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2559 3728 5112 5380 1632
Size: 927 1169 1384 268 1917
Fragments arranged by size:
1917 1384 1169 927 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5468
AflIII A'CryG_T
Cuts at: 1203 2728 2918 4564 5541 1203
Size: 1525 190 1646 977 1327
Fragments arranged by size:
1646 1525 1327 977 190
AgeI A'CCGG_T
Cuts at: 4496 4496
Size: 5665
AhdI GACnn_n'nnGTC
Cuts at: 4394 4394
Size: 5665
AleI CACnn'nnGTG
Cuts at: 1166 2228 1166
Size: 1062 4603
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2496 1184
Size: 1312 4353
AlwNI CAG_nnn'CTG
Cuts at: 3408 4980 3408
Size: 1572 4093
ApaLI G'TGCA_C
Cuts at: 2508 3722 4878 2508
Size: 1214 1156 3295
Fragments arranged by size:
3295 1214 1156
ApoI r'AATT_y
Cuts at: 1493 2974 3200 3352 5461 1493
Size: 1481 226 152 2109 1697
Fragments arranged by size:
2109 1697 1481 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4219 160
Size: 872 3187 1606
Fragments arranged by size:
3187 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5665
AvaI C'yCGr_G
Cuts at: 1870 1943 2990 1870
Size: 73 1047 4545
Fragments arranged by size:
4545 1047 73
AvrII C'CTAG_G
Cuts at: 2041 2041
Size: 5665
BamHI G'GATC_C
Cuts at: 2995 3362 2995
Size: 367 5298
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2986 4441 618
Size: 325 217 627 1199 1455 1842
Fragments arranged by size:
1842 1455 1199 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2068 2984 1391
Size: 677 916 4072
Fragments arranged by size:
4072 916 677
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2572 928
Size: 971 673 4021
Fragments arranged by size:
4021 971 673
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3856 3890 1903
Size: 34 1919 34 3678
Fragments arranged by size:
3678 1919 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3557 4767 3557
Size: 1210 4455
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5665
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4276 136
Size: 107 122 71 631 605 2604 1525
Fragments arranged by size:
2604 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2298 5660 2298
Size: 3362 2303
BlpI GC'TnA_GC
Cuts at: 3082 3082
Size: 5665
Bme1580I G_kGCm'C
Cuts at: 1790 2235 2512 3726 4882 1790
Size: 445 277 1214 1156 2573
Fragments arranged by size:
2573 1214 1156 445 277
BmgBI CAC'GTC
Cuts at: 1318 2209 2919 1318
Size: 891 710 4064
Fragments arranged by size:
4064 891 710
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2158 2327 3396
Size: 416 53 83 399 24 741 169 1069
Cuts at: 3396 4354 442
Size: 958 1753
Fragments arranged by size:
1753 1069 958 741 416 399 169 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5665
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4325 1239
Size: 3086 2579
Bpu10I CC'TnA_GC
Cuts at: 2186 2489 2186
Size: 303 5362
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3790 4655 4953 3790
Size: 865 298 4502
Fragments arranged by size:
4502 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2660 2773 4328 882
Size: 617 1161 113 1555 2219
Fragments arranged by size:
2219 1555 1161 617 113
BsaAI yAC'GTr
Cuts at: 493 1204 3456 493
Size: 711 2252 2702
Fragments arranged by size:
2702 2252 711
BsaBI GATnn'nnATC
Cuts at: 5659 5659
Size: 5665
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3472 3854 4489 275
Size: 53 83 186 936 1939 382 635 1451
Fragments arranged by size:
1939 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2258 4098 4496 4770 4917 5328 2258
Size: 1840 398 274 147 411 2595
Fragments arranged by size:
2595 1840 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5440
Fragments arranged by size:
5440 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5665
BseYI C'CCAG_C
Cuts at: 2911 4868 5364 2911
Size: 1957 496 3212
Fragments arranged by size:
3212 1957 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2315 2475 1163
Size: 573 579 160 4353
Fragments arranged by size:
4353 579 573 160
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3115 3876 4904 1540
Size: 275 186 1114 761 1028 2301
Fragments arranged by size:
2301 1114 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2512 3726 3811 4882 2512
Size: 1214 85 1071 3295
Fragments arranged by size:
3295 1214 1071 85
BsmI GAATG_Cn'
Cuts at: 3176 3269 3176
Size: 93 5572
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2068 2235 2512 2984 3726 3811 4882
Size: 399 278 167 277 472 742 85 1071
Cuts at: 4882 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 472 399 278 277 167
85
BspEI T'CCGG_A
Cuts at: 5328 5328
Size: 5665
BspHI T'CATG_A
Cuts at: 2082 2130 2403 3554 5284 2082
Size: 48 273 1151 1730 2463
Fragments arranged by size:
2463 1730 1151 273 48
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2726 3020 844
Size: 1882 294 3489
Fragments arranged by size:
3489 1882 294
BsrBI CCG'CTC
Cuts at: 1394 1823 3552 1394
Size: 429 1729 3507
Fragments arranged by size:
3507 1729 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2128 4160 4334 65
Size: 1183 880 2032 174 1396
Fragments arranged by size:
2032 1396 1183 880 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2157 4309 4496 1700
Size: 110 347 2152 187 2869
Fragments arranged by size:
2869 2152 347 187 110
BsrGI T'GTAC_A
Cuts at: 96 2595 96
Size: 2499 3166
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5665
BssSI C'ACGA_G
Cuts at: 2513 3725 4737 2513
Size: 1212 1012 3441
Fragments arranged by size:
3441 1212 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5665
BstBI TT'CG_AA
Cuts at: 2583 4469 2583
Size: 1886 3779
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5665
BstYI r'GATC_y
Cuts at: 1972 2298 2995 3362 3747 3764 5216 5302 5314
Size: 326 697 367 385 17 1452 86 12
Cuts at: 5314 5660 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 697 385 367 346 326 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2423 2423
Size: 5665
BtgI C'CryG_G
Cuts at: 513 1065 2004 2349 513
Size: 552 939 345 3829
Fragments arranged by size:
3829 939 552 345
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4926
Fragments arranged by size:
4926 683 56
BtsI GCAGTG_nn'
Cuts at: 3181 3975 3995 3181
Size: 794 20 4851
Fragments arranged by size:
4851 794 20
ClaI AT'CG_AT
Cuts at: 1554 2520 1554
Size: 966 4699
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5630
DraI TTT'AAA
Cuts at: 2969 2969
Size: 5665
DraIII CAC_nnn'GTG
Cuts at: 2321 2321
Size: 5665
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2437 2576 4672 809
Size: 507 1121 139 2096 1802
Fragments arranged by size:
2096 1802 1121 507 139
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2094 3112 4001
Size: 54 1425 50 275 18 264 1018 889
Cuts at: 4001 5630 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1018 889 275 264 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3112 1537
Size: 275 1300 4090
Fragments arranged by size:
4090 1300 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2084 2728 3595 1353
Size: 731 644 867 3423
Fragments arranged by size:
3423 867 731 644
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4252 4626 4772 387
Size: 1064 138 2663 374 146 1280
Fragments arranged by size:
2663 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2559 3728 4325 5112 5380 1239
Size: 393 927 1169 597 787 268 1524
Fragments arranged by size:
1524 1169 927 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2471 2471
Size: 5665
EcoO109I rG'GnC_Cy
Cuts at: 2539 2539
Size: 5665
EcoRI G'AATT_C
Cuts at: 2974 3352 2974
Size: 378 5287
EcoRV GAT'ATC
Cuts at: 2393 2393
Size: 5665
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2725 2725
Size: 5665
FspI TGC'GCA
Cuts at: 4171 4171
Size: 5665
HaeII r_GCGC'y
Cuts at: 1678 4812 1678
Size: 3134 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4306 4338 4380 4412 1373
Size: 32 135 32 2734 32 42 32 2626
Fragments arranged by size:
2734 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3009 3255 669
Size: 988 281 1071 246 3079
Fragments arranged by size:
3079 1071 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5665
HpaI GTT'AAC
Cuts at: 3255 3255
Size: 5665
KpnI G_GTAC'C
Cuts at: 2990 2990
Size: 5665
MfeI C'AATT_G
Cuts at: 3264 3264
Size: 5665
MluI A'CGCG_T
Cuts at: 5541 5541
Size: 5665
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4779 4963 1305
Size: 3474 184 2007
Fragments arranged by size:
3474 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2096 10
Size: 54 1425 343 264 3579
Fragments arranged by size:
3579 1425 343 264 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2171 2207 2228 2402 3623 3982
Size: 648 843 162 36 21 174 1221 359
Cuts at: 3982 4141 518
Size: 159 2042
Fragments arranged by size:
2042 1221 843 648 359 174 162 159
36 21
MspA1I CmG'CkG
Cuts at: 1606 2416 2459 3081 3758 4906 5151 1606
Size: 810 43 622 677 1148 245 2120
Fragments arranged by size:
2120 1148 810 677 622 245 43
NcoI C'CATG_G
Cuts at: 513 1065 2004 2349 513
Size: 552 939 345 3829
Fragments arranged by size:
3829 939 552 345
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5665
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5665
NotI GC'GGCC_GC
Cuts at: 3112 3112
Size: 5665
NspI r_CATG'y
Cuts at: 1132 1229 2732 3023 4568 1132
Size: 97 1503 291 1545 2229
Fragments arranged by size:
2229 1545 1503 291 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5665
PciI A'CATG_T
Cuts at: 2728 4564 2728
Size: 1836 3829
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5665
PmeI GTTT'AAAC
Cuts at: 2969 2969
Size: 5665
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3734 3766 5247 5279 3734
Size: 32 1481 32 4120
Fragments arranged by size:
4120 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5665
PsiI TTA'TAA
Cuts at: 3235 3235
Size: 5665
PstI C_TGCA'G
Cuts at: 830 2475 3017 830
Size: 1645 542 3478
Fragments arranged by size:
3478 1645 542
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5665
PvuII CAG'CTG
Cuts at: 2416 2459 2416
Size: 43 5622
SalI G'TCGA_C
Cuts at: 1936 3007 1936
Size: 1071 4594
SapI GCTCTTCn'nnn_
Cuts at: 2728 2728
Size: 5665
SbfI CC_TGCA'GG
Cuts at: 2475 3017 2475
Size: 542 5123
ScaI AGT'ACT
Cuts at: 3913 5625 3913
Size: 1712 3953
SfcI C'TryA_G
Cuts at: 826 1045 2471 2688 2866 3013 4148 4829 5020
Size: 219 1426 217 178 147 1135 681 191
Cuts at: 5020 826
Size: 1471
Fragments arranged by size:
1471 1426 1135 681 219 217 191 178
147
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5665
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5665
SmaI CCC'GGG
Cuts at: 1872 2992 1872
Size: 1120 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3769 4670 4932 820
Size: 197 926 1826 901 262 1553
Fragments arranged by size:
1826 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3456 493
Size: 2963 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5665
SphI G_CATG'C
Cuts at: 1132 3023 1132
Size: 1891 3774
SspI AAT'ATT
Cuts at: 5 52 3589 5
Size: 47 3537 2081
Fragments arranged by size:
3537 2081 47
StuI AGG'CCT
Cuts at: 1517 2708 1517
Size: 1191 4474
StyI C'CwwG_G
Cuts at: 513 1065 2004 2041 2349 3104 513
Size: 552 939 37 308 755 3074
Fragments arranged by size:
3074 939 755 552 308 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2309 2397 2441 3692 3709 3862 4047
Size: 1255 729 88 44 1251 17 153 185
Cuts at: 4047 4492 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1251 729 445 185 153 88
44 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2175 2595 3911
Size: 274 80 33 51 1443 198 420 1316
Cuts at: 3911 5623 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1316 420 274 198 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5625 5657 5625
Size: 32 5633
Tth111I GACn'n_nGTC
Cuts at: 1316 2434 1316
Size: 1118 4547
XbaI T'CTAG_A
Cuts at: 3001 3001
Size: 5665
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5665
XmaI C'CCGG_G
Cuts at: 1870 2990 1870
Size: 1120 4545
XmnI GAAnn'nnTTC
Cuts at: 3794 3794
Size: 5665
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3473 276
Size: 53 83 186 936 1939 2468
Fragments arranged by size:
2468 1939 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I EcoICRI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SexAI SfiI SfoI SrfI SwaI XcmI