Restriction map for
pFN21AE6059
(Circular) (Six-base) MAPSORT of: pFN21AE6059.seq Check: 3099 from: 1 to: 5665
pFN21AE6059 5665 bp
With 182 enzymes: SgfI *
December 8, 2014 13:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2738 2738
Size: 5665
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3475 278
Size: 53 83 186 936 1939 2468
Fragments arranged by size:
2468 1939 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3008 1937
Size: 1071 4594
Acc65I G'GTAC_C
Cuts at: 2986 2986
Size: 5665
AclI AA'CG_TT
Cuts at: 1563 3792 4165 1563
Size: 2229 373 3063
Fragments arranged by size:
3063 2229 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2707 3728 5112 5380 1632
Size: 1075 1021 1384 268 1917
Fragments arranged by size:
1917 1384 1075 1021 268
AflII C'TTAA_G
Cuts at: 820 1017 2936 820
Size: 197 1919 3549
Fragments arranged by size:
3549 1919 197
AflIII A'CryG_T
Cuts at: 1203 2553 4564 5541 1203
Size: 1350 2011 977 1327
Fragments arranged by size:
2011 1350 1327 977
AgeI A'CCGG_T
Cuts at: 4496 4496
Size: 5665
AhdI GACnn_n'nnGTC
Cuts at: 2554 4394 2554
Size: 1840 3825
AleI CACnn'nnGTG
Cuts at: 1166 2219 2745 1166
Size: 1053 526 4086
Fragments arranged by size:
4086 1053 526
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5665
AlwNI CAG_nnn'CTG
Cuts at: 2730 3408 4980 2730
Size: 678 1572 3415
Fragments arranged by size:
3415 1572 678
ApaLI G'TGCA_C
Cuts at: 2230 2321 3722 4878 2230
Size: 91 1401 1156 3017
Fragments arranged by size:
3017 1401 1156 91
ApoI r'AATT_y
Cuts at: 1493 2974 3200 3352 5461 1493
Size: 1481 226 152 2109 1697
Fragments arranged by size:
2109 1697 1481 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4219 160
Size: 872 3187 1606
Fragments arranged by size:
3187 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5665
AvaI C'yCGr_G
Cuts at: 1870 1943 2990 1870
Size: 73 1047 4545
Fragments arranged by size:
4545 1047 73
BamHI G'GATC_C
Cuts at: 2995 3362 2995
Size: 367 5298
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2021 2986 4441 618
Size: 325 217 627 234 965 1455 1842
Fragments arranged by size:
1842 1455 965 627 325 234 217
BanII G_rGCy'C
Cuts at: 1391 2984 1391
Size: 1593 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2185 928
Size: 971 286 4408
Fragments arranged by size:
4408 971 286
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3856 3890 1903
Size: 34 1919 34 3678
Fragments arranged by size:
3678 1919 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3557 4767 3557
Size: 1210 4455
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5665
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4276 136
Size: 107 122 71 631 605 2604 1525
Fragments arranged by size:
2604 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5660 5660
Size: 5665
BlpI GC'TnA_GC
Cuts at: 3082 3082
Size: 5665
Bme1580I G_kGCm'C
Cuts at: 1790 2234 2325 3726 4882 1790
Size: 444 91 1401 1156 2573
Fragments arranged by size:
2573 1401 1156 444 91
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5665
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3396 4354 442
Size: 416 53 83 399 24 1979 958 1753
Fragments arranged by size:
1979 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5665
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2019 4325 1239
Size: 780 2306 2579
Fragments arranged by size:
2579 2306 780
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2261 3790 4655 4953 2261
Size: 1529 865 298 2973
Fragments arranged by size:
2973 1529 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4328 882
Size: 617 2829 2219
Fragments arranged by size:
2829 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3456 493
Size: 711 2252 2702
Fragments arranged by size:
2702 2252 711
BsaBI GATnn'nnATC
Cuts at: 5659 5659
Size: 5665
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3472 3854 4489 275
Size: 53 83 186 936 1939 382 635 1451
Fragments arranged by size:
1939 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4098 4496 4770 4917 5328 4098
Size: 398 274 147 411 4435
Fragments arranged by size:
4435 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5440
Fragments arranged by size:
5440 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5665
BseYI C'CCAG_C
Cuts at: 2441 4868 5364 2441
Size: 2427 496 2742
Fragments arranged by size:
2742 2427 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2716 1163
Size: 573 980 4112
Fragments arranged by size:
4112 980 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3115 3876 4904 1540
Size: 275 186 1114 761 1028 2301
Fragments arranged by size:
2301 1114 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2226 2234 2325 2355 3726 3811 4882 2226
Size: 8 91 30 1371 85 1071 3009
Fragments arranged by size:
3009 1371 1071 91 85 30 8
BsmI GAATG_Cn'
Cuts at: 3176 3269 3176
Size: 93 5572
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2226 2234 2325 2355 2984 3726 3811
Size: 399 436 8 91 30 629 742 85
Cuts at: 3811 4882 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 629 436 399 91 85
30 8
BspEI T'CCGG_A
Cuts at: 5328 5328
Size: 5665
BspHI T'CATG_A
Cuts at: 3554 5284 3554
Size: 1730 3935
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2738 2896 3020 844
Size: 1894 158 124 3489
Fragments arranged by size:
3489 1894 158 124
BsrBI CCG'CTC
Cuts at: 1394 1823 3552 1394
Size: 429 1729 3507
Fragments arranged by size:
3507 1729 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2118 2430 4160 4334 65
Size: 1183 870 312 1730 174 1396
Fragments arranged by size:
1730 1396 1183 870 312 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4309 4496 1700
Size: 110 2499 187 2869
Fragments arranged by size:
2869 2499 187 110
BsrGI T'GTAC_A
Cuts at: 96 2112 96
Size: 2016 3649
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5665
BssSI C'ACGA_G
Cuts at: 3725 4737 3725
Size: 1012 4653
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5665
BstBI TT'CG_AA
Cuts at: 4469 4469
Size: 5665
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5665
BstYI r'GATC_y
Cuts at: 1972 2088 2995 3362 3747 3764 5216 5302 5314
Size: 116 907 367 385 17 1452 86 12
Cuts at: 5314 5660 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 907 385 367 346 116 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5113
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4926
Fragments arranged by size:
4926 683 56
BtsI GCAGTG_nn'
Cuts at: 2733 3181 3975 3995 2733
Size: 448 794 20 4403
Fragments arranged by size:
4403 794 448 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5665
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5630
DraI TTT'AAA
Cuts at: 2293 2969 2293
Size: 676 4989
DraIII CAC_nnn'GTG
Cuts at: 2380 2502 2380
Size: 122 5543
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4672 809
Size: 507 3356 1802
Fragments arranged by size:
3356 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2382 2562 3112
Size: 54 1425 50 275 18 552 180 550
Cuts at: 3112 4001 5630 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 552 550 275 180 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3112 1537
Size: 275 1300 4090
Fragments arranged by size:
4090 1300 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2261 3595 1353
Size: 908 1334 3423
Fragments arranged by size:
3423 1334 908
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4252 4626 4772 387
Size: 1064 138 2663 374 146 1280
Fragments arranged by size:
2663 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2019 2707 3728 4325 5112 5380 1239
Size: 393 387 688 1021 597 787 268 1524
Fragments arranged by size:
1524 1021 787 688 597 393 387 268
EcoRI G'AATT_C
Cuts at: 2974 3352 2974
Size: 378 5287
EcoRV GAT'ATC
Cuts at: 2571 2641 2571
Size: 70 5595
FspI TGC'GCA
Cuts at: 4171 4171
Size: 5665
HaeII r_GCGC'y
Cuts at: 1678 4812 1678
Size: 3134 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2540 2572 2904 2936 4306
Size: 32 135 32 968 32 332 32 1370
Cuts at: 4306 4338 4380 4412 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1370 968 332 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2593 3009 3255 669
Size: 988 281 655 416 246 3079
Fragments arranged by size:
3079 988 655 416 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5665
HpaI GTT'AAC
Cuts at: 2593 3255 2593
Size: 662 5003
KpnI G_GTAC'C
Cuts at: 2990 2990
Size: 5665
MfeI C'AATT_G
Cuts at: 3264 3264
Size: 5665
MluI A'CGCG_T
Cuts at: 5541 5541
Size: 5665
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4779 4963 1305
Size: 3474 184 2007
Fragments arranged by size:
3474 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2384 2564 10
Size: 54 1425 343 552 180 3111
Fragments arranged by size:
3111 1425 552 343 180 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2219 2336 2360 2558 2745 3623 3982
Size: 648 1053 117 24 198 187 878 359
Cuts at: 3982 4141 518
Size: 159 2042
Fragments arranged by size:
2042 1053 878 648 359 198 187 159
117 24
MspA1I CmG'CkG
Cuts at: 1606 3081 3758 4906 5151 1606
Size: 1475 677 1148 245 2120
Fragments arranged by size:
2120 1475 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5113
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5665
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5665
NotI GC'GGCC_GC
Cuts at: 3112 3112
Size: 5665
NspI r_CATG'y
Cuts at: 1132 1229 2557 3023 4568 1132
Size: 97 1328 466 1545 2229
Fragments arranged by size:
2229 1545 1328 466 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5665
PciI A'CATG_T
Cuts at: 2553 4564 2553
Size: 2011 3654
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2380 1108
Size: 1272 4393
PmeI GTTT'AAAC
Cuts at: 2969 2969
Size: 5665
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3734 3766 5247 5279 3734
Size: 32 1481 32 4120
Fragments arranged by size:
4120 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5665
PsiI TTA'TAA
Cuts at: 3235 3235
Size: 5665
PstI C_TGCA'G
Cuts at: 830 3017 830
Size: 2187 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5665
SalI G'TCGA_C
Cuts at: 1936 3007 1936
Size: 1071 4594
SbfI CC_TGCA'GG
Cuts at: 3017 3017
Size: 5665
ScaI AGT'ACT
Cuts at: 3913 5625 3913
Size: 1712 3953
SfcI C'TryA_G
Cuts at: 826 1045 3013 4148 4829 5020 826
Size: 219 1968 1135 681 191 1471
Fragments arranged by size:
1968 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5665
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5665
SmaI CCC'GGG
Cuts at: 1872 2992 1872
Size: 1120 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2276 2936 3769 4670 4932 820
Size: 197 926 333 660 833 901 262 1553
Fragments arranged by size:
1553 926 901 833 660 333 262 197
SnaBI TAC'GTA
Cuts at: 493 3456 493
Size: 2963 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5665
SphI G_CATG'C
Cuts at: 1132 3023 1132
Size: 1891 3774
SspI AAT'ATT
Cuts at: 5 52 3589 5
Size: 47 3537 2081
Fragments arranged by size:
3537 2081 47
StuI AGG'CCT
Cuts at: 1517 2171 2720 1517
Size: 654 549 4462
Fragments arranged by size:
4462 654 549
StyI C'CwwG_G
Cuts at: 513 1065 3104 513
Size: 552 2039 3074
Fragments arranged by size:
3074 2039 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3692 3709 3862 4047 4492 325
Size: 1255 2112 17 153 185 445 1498
Fragments arranged by size:
2112 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2112 3911 5623
Size: 274 80 33 51 1443 135 1799 1712
Cuts at: 5623 96
Size: 138
Fragments arranged by size:
1799 1712 1443 274 138 135 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2752 2784 5625 5657 2752
Size: 32 2841 32 2760
Fragments arranged by size:
2841 2760 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5665
XbaI T'CTAG_A
Cuts at: 3001 3001
Size: 5665
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5665
XmaI C'CCGG_G
Cuts at: 1870 2990 1870
Size: 1120 4545
XmnI GAAnn'nnTTC
Cuts at: 2720 3794 2720
Size: 1074 4591
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3473 276
Size: 53 83 186 936 1939 2468
Fragments arranged by size:
2468 1939 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI