Restriction map for
pFN21AE6058
(Circular) (Six-base) MAPSORT of: pFN21AE6058.seq Check: 6812 from: 1 to: 5665
pFN21AE6058 5665 bp
With 182 enzymes: SgfI *
December 8, 2014 13:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2884 2884
Size: 5665
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3475 278
Size: 53 83 186 936 1939 2468
Fragments arranged by size:
2468 1939 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3008 1937
Size: 1071 4594
Acc65I G'GTAC_C
Cuts at: 2986 2986
Size: 5665
AclI AA'CG_TT
Cuts at: 1563 3792 4165 1563
Size: 2229 373 3063
Fragments arranged by size:
3063 2229 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3728 5112 5380 1632
Size: 2096 1384 268 1917
Fragments arranged by size:
2096 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5468
AflIII A'CryG_T
Cuts at: 1203 2738 4564 5541 1203
Size: 1535 1826 977 1327
Fragments arranged by size:
1826 1535 1327 977
AgeI A'CCGG_T
Cuts at: 4496 4496
Size: 5665
AhdI GACnn_n'nnGTC
Cuts at: 4394 4394
Size: 5665
AleI CACnn'nnGTG
Cuts at: 1166 2754 1166
Size: 1588 4077
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5665
AlwNI CAG_nnn'CTG
Cuts at: 3408 4980 3408
Size: 1572 4093
ApaI G_GGCC'C
Cuts at: 2118 2286 2905 2118
Size: 168 619 4878
Fragments arranged by size:
4878 619 168
ApaLI G'TGCA_C
Cuts at: 2556 2837 3722 4878 2556
Size: 281 885 1156 3343
Fragments arranged by size:
3343 1156 885 281
ApoI r'AATT_y
Cuts at: 1493 2974 3200 3352 5461 1493
Size: 1481 226 152 2109 1697
Fragments arranged by size:
2109 1697 1481 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4219 160
Size: 872 3187 1606
Fragments arranged by size:
3187 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5665
AvaI C'yCGr_G
Cuts at: 1870 1943 2709 2990 1870
Size: 73 766 281 4545
Fragments arranged by size:
4545 766 281 73
BamHI G'GATC_C
Cuts at: 2995 3362 2995
Size: 367 5298
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2986 4441 618
Size: 325 217 627 1199 1455 1842
Fragments arranged by size:
1842 1455 1199 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2118 2286 2905 2984 1391
Size: 727 168 619 79 4072
Fragments arranged by size:
4072 727 619 168 79
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4694
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3856 3890 1903
Size: 34 1919 34 3678
Fragments arranged by size:
3678 1919 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3557 4767 3557
Size: 1210 4455
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5665
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2216 4276 136
Size: 107 122 71 631 605 544 2060 1525
Fragments arranged by size:
2060 1525 631 605 544 122 107 71
BglII A'GATC_T
Cuts at: 5660 5660
Size: 5665
BlpI GC'TnA_GC
Cuts at: 2236 3082 2236
Size: 846 4819
Bme1580I G_kGCm'C
Cuts at: 1790 2089 2118 2286 2560 2841 2905 3726 4882
Size: 299 29 168 274 281 64 821 1156
Cuts at: 4882 1790
Size: 2573
Fragments arranged by size:
2573 1156 821 299 281 274 168 64
29
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5665
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3396 4354 442
Size: 416 53 83 399 24 1979 958 1753
Fragments arranged by size:
1979 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5665
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2945 2945
Size: 5665
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4325 1239
Size: 3086 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3790 4655 4953 3790
Size: 865 298 4502
Fragments arranged by size:
4502 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2789 2916 4328 882
Size: 617 1290 127 1412 2219
Fragments arranged by size:
2219 1412 1290 617 127
BsaAI yAC'GTr
Cuts at: 493 1204 3456 493
Size: 711 2252 2702
Fragments arranged by size:
2702 2252 711
BsaBI GATnn'nnATC
Cuts at: 5659 5659
Size: 5665
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2401 3472 3854 4489
Size: 53 83 186 936 868 1071 382 635
Cuts at: 4489 275
Size: 1451
Fragments arranged by size:
1451 1071 936 868 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4098 4496 4770 4917 5328 4098
Size: 398 274 147 411 4435
Fragments arranged by size:
4435 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5440
Fragments arranged by size:
5440 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5665
BseYI C'CCAG_C
Cuts at: 2137 4868 5364 2137
Size: 2731 496 2438
Fragments arranged by size:
2731 2438 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2168 2393 1163
Size: 573 432 225 4435
Fragments arranged by size:
4435 573 432 225
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3115 3876 4904 1540
Size: 275 186 1114 761 1028 2301
Fragments arranged by size:
2301 1114 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2152 2523 2560 2841 3726 3811 4882 2152
Size: 371 37 281 885 85 1071 2935
Fragments arranged by size:
2935 1071 885 371 281 85 37
BsmI GAATG_Cn'
Cuts at: 3176 3269 3176
Size: 93 5572
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2089 2118 2152 2286 2523 2560 2841
Size: 399 299 29 34 134 237 37 281
Cuts at: 2841 2905 2984 3726 3811 4882 1391
Size: 64 79 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 299 281 237 134
85 79 64 37 34 29
BspEI T'CCGG_A
Cuts at: 5328 5328
Size: 5665
BspHI T'CATG_A
Cuts at: 3554 5284 3554
Size: 1730 3935
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2884 3020 844
Size: 2040 136 3489
Fragments arranged by size:
3489 2040 136
BsrBI CCG'CTC
Cuts at: 1394 1823 3552 1394
Size: 429 1729 3507
Fragments arranged by size:
3507 1729 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2149 2679 4160 4334 65
Size: 1183 901 530 1481 174 1396
Fragments arranged by size:
1481 1396 1183 901 530 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2155 4309 4496 1700
Size: 110 345 2154 187 2869
Fragments arranged by size:
2869 2154 345 187 110
BsrGI T'GTAC_A
Cuts at: 96 2329 96
Size: 2233 3432
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5665
BssSI C'ACGA_G
Cuts at: 3725 4737 3725
Size: 1012 4653
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2342 794
Size: 1548 4117
BstBI TT'CG_AA
Cuts at: 4469 4469
Size: 5665
BstEII G'GTnAC_C
Cuts at: 1184 2425 1184
Size: 1241 4424
BstXI CCAn_nnnn'nTGG
Cuts at: 2230 2230
Size: 5665
BstYI r'GATC_y
Cuts at: 1972 2995 3362 3747 3764 5216 5302 5314 5660
Size: 1023 367 385 17 1452 86 12 346
Cuts at: 5660 1972
Size: 1977
Fragments arranged by size:
1977 1452 1023 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2346 2370 513
Size: 552 939 342 24 3808
Fragments arranged by size:
3808 939 552 342 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4926
Fragments arranged by size:
4926 683 56
BtsI GCAGTG_nn'
Cuts at: 2150 2166 2180 2405 2498 2574 3181 3975 3995
Size: 16 14 225 93 76 607 794 20
Cuts at: 3995 2150
Size: 3820
Fragments arranged by size:
3820 794 607 225 93 76 20 16
14
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5665
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5630
DraI TTT'AAA
Cuts at: 2969 2969
Size: 5665
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4672 809
Size: 507 3356 1802
Fragments arranged by size:
3356 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2208 2367 2631
Size: 54 1425 50 275 18 378 159 264
Cuts at: 2631 3112 4001 5630 8
Size: 481 889 1629 43
Fragments arranged by size:
1629 1425 889 481 378 275 264 159
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3112 1537
Size: 275 1300 4090
Fragments arranged by size:
4090 1300 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2792 3595 1353
Size: 1439 803 3423
Fragments arranged by size:
3423 1439 803
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4252 4626 4772 387
Size: 1064 138 2663 374 146 1280
Fragments arranged by size:
2663 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3728 4325 5112 5380 1239
Size: 393 2096 597 787 268 1524
Fragments arranged by size:
2096 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2283 2901 2283
Size: 618 5047
EcoRI G'AATT_C
Cuts at: 2974 3352 2974
Size: 378 5287
FspI TGC'GCA
Cuts at: 4171 4171
Size: 5665
HaeII r_GCGC'y
Cuts at: 1678 4812 1678
Size: 3134 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4306 4338 4380 4412 1373
Size: 32 135 32 2734 32 42 32 2626
Fragments arranged by size:
2734 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3009 3255 669
Size: 988 281 1071 246 3079
Fragments arranged by size:
3079 1071 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5665
HpaI GTT'AAC
Cuts at: 3255 3255
Size: 5665
KpnI G_GTAC'C
Cuts at: 2990 2990
Size: 5665
MfeI C'AATT_G
Cuts at: 3264 3264
Size: 5665
MluI A'CGCG_T
Cuts at: 5541 5541
Size: 5665
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2877 4779 4963 1305
Size: 1572 1902 184 2007
Fragments arranged by size:
2007 1902 1572 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2210 2369 2633 10
Size: 54 1425 343 378 159 264 3042
Fragments arranged by size:
3042 1425 378 343 264 159 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2567 2754 3623 3982 4141 518
Size: 648 1401 187 869 359 159 2042
Fragments arranged by size:
2042 1401 869 648 359 187 159
MspA1I CmG'CkG
Cuts at: 1606 3081 3758 4906 5151 1606
Size: 1475 677 1148 245 2120
Fragments arranged by size:
2120 1475 1148 677 245
NaeI GCC'GGC
Cuts at: 2157 2157
Size: 5665
NcoI C'CATG_G
Cuts at: 513 1065 2004 2346 2370 513
Size: 552 939 342 24 3808
Fragments arranged by size:
3808 939 552 342 24
NdeI CA'TA_TG
Cuts at: 387 2299 387
Size: 1912 3753
NgoMIV G'CCGG_C
Cuts at: 2155 2155
Size: 5665
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5665
NotI GC'GGCC_GC
Cuts at: 3112 3112
Size: 5665
NspI r_CATG'y
Cuts at: 1132 1229 2336 2742 3023 4568 1132
Size: 97 1107 406 281 1545 2229
Fragments arranged by size:
2229 1545 1107 406 281 97
PasI CC'CwG_GG
Cuts at: 1348 2255 1348
Size: 907 4758
PciI A'CATG_T
Cuts at: 2738 4564 2738
Size: 1826 3839
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5665
PfoI T'CCnGG_A
Cuts at: 2931 2931
Size: 5665
PmeI GTTT'AAAC
Cuts at: 2969 2969
Size: 5665
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3734 3766 5247 5279 3734
Size: 32 1481 32 4120
Fragments arranged by size:
4120 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2105 1613
Size: 492 5173
PsiI TTA'TAA
Cuts at: 3235 3235
Size: 5665
PspOMI G'GGCC_C
Cuts at: 2114 2282 2901 2114
Size: 168 619 4878
Fragments arranged by size:
4878 619 168
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2728 2760 2728
Size: 32 5633
PstI C_TGCA'G
Cuts at: 830 2700 3017 830
Size: 1870 317 3478
Fragments arranged by size:
3478 1870 317
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5665
SalI G'TCGA_C
Cuts at: 1936 3007 1936
Size: 1071 4594
SbfI CC_TGCA'GG
Cuts at: 3017 3017
Size: 5665
ScaI AGT'ACT
Cuts at: 3913 5625 3913
Size: 1712 3953
SexAI A'CCwGG_T
Cuts at: 2779 2779
Size: 5665
SfcI C'TryA_G
Cuts at: 826 1045 2247 2696 3013 4148 4829 5020 826
Size: 219 1202 449 317 1135 681 191 1471
Fragments arranged by size:
1471 1202 1135 681 449 317 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5665
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5665
SmaI CCC'GGG
Cuts at: 1872 2711 2992 1872
Size: 839 281 4545
Fragments arranged by size:
4545 839 281
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3769 4670 4932 820
Size: 197 926 1826 901 262 1553
Fragments arranged by size:
1826 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3456 493
Size: 2963 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5665
SphI G_CATG'C
Cuts at: 1132 3023 1132
Size: 1891 3774
SspI AAT'ATT
Cuts at: 5 52 3589 5
Size: 47 3537 2081
Fragments arranged by size:
3537 2081 47
StuI AGG'CCT
Cuts at: 1517 2729 2768 1517
Size: 1212 39 4414
Fragments arranged by size:
4414 1212 39
StyI C'CwwG_G
Cuts at: 513 1065 2004 2346 2370 2454 2724 3104 513
Size: 552 939 342 24 84 270 380 3074
Fragments arranged by size:
3074 939 552 380 342 270 84 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2058 2940 3692 3709 3862 4047 4492
Size: 1255 478 882 752 17 153 185 445
Cuts at: 4492 325
Size: 1498
Fragments arranged by size:
1498 1255 882 752 478 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2329 3911 5623
Size: 274 80 33 51 1443 352 1582 1712
Cuts at: 5623 96
Size: 138
Fragments arranged by size:
1712 1582 1443 352 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5625 5657 5625
Size: 32 5633
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5665
XbaI T'CTAG_A
Cuts at: 3001 3001
Size: 5665
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2125 2437 2641 2125
Size: 312 204 5149
Fragments arranged by size:
5149 312 204
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5665
XmaI C'CCGG_G
Cuts at: 1870 2709 2990 1870
Size: 839 281 4545
Fragments arranged by size:
4545 839 281
XmnI GAAnn'nnTTC
Cuts at: 3794 3794
Size: 5665
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3473 276
Size: 53 83 186 936 1939 2468
Fragments arranged by size:
2468 1939 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PsrI PstI PvuI SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PmlI PpuMI PspXI PvuII RsrII SacI SacII
SanDI SapI SfiI SfoI SrfI SwaI