Restriction map for
pFN21AE6053
(Circular) (Six-base) MAPSORT of: pFN21AE6053.seq Check: 490 from: 1 to: 5659
pFN21AE6053 5659 bp
With 182 enzymes: SgfI *
December 8, 2014 13:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2729 2729
Size: 5659
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3469 278
Size: 53 83 186 936 1933 2468
Fragments arranged by size:
2468 1933 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3002 1937
Size: 1065 4594
Acc65I G'GTAC_C
Cuts at: 2980 2980
Size: 5659
AclI AA'CG_TT
Cuts at: 1563 3786 4159 1563
Size: 2223 373 3063
Fragments arranged by size:
3063 2223 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2699 3722 5106 5374 1632
Size: 1067 1023 1384 268 1917
Fragments arranged by size:
1917 1384 1067 1023 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5462
AflIII A'CryG_T
Cuts at: 1203 2211 4558 5535 1203
Size: 1008 2347 977 1327
Fragments arranged by size:
2347 1327 1008 977
AgeI A'CCGG_T
Cuts at: 4490 4490
Size: 5659
AhdI GACnn_n'nnGTC
Cuts at: 4388 4388
Size: 5659
AleI CACnn'nnGTG
Cuts at: 1166 2736 1166
Size: 1570 4089
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5659
AlwNI CAG_nnn'CTG
Cuts at: 3402 4974 3402
Size: 1572 4087
ApaI G_GGCC'C
Cuts at: 2514 2514
Size: 5659
ApaLI G'TGCA_C
Cuts at: 2422 3716 4872 2422
Size: 1294 1156 3209
Fragments arranged by size:
3209 1294 1156
ApoI r'AATT_y
Cuts at: 1493 2481 2541 2968 3194 3346 5455 1493
Size: 988 60 427 226 152 2109 1697
Fragments arranged by size:
2109 1697 988 427 226 152 60
AseI AT'TA_AT
Cuts at: 160 1032 4213 160
Size: 872 3181 1606
Fragments arranged by size:
3181 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5659
AvaI C'yCGr_G
Cuts at: 1870 1943 2514 2666 2984 1870
Size: 73 571 152 318 4545
Fragments arranged by size:
4545 571 318 152 73
BamHI G'GATC_C
Cuts at: 2989 3356 2989
Size: 367 5292
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2897 2980 4435 618
Size: 325 217 627 1110 83 1455 1842
Fragments arranged by size:
1842 1455 1110 627 325 217 83
BanII G_rGCy'C
Cuts at: 1391 2514 2978 1391
Size: 1123 464 4072
Fragments arranged by size:
4072 1123 464
BbeI G_GCGC'C
Cuts at: 2901 2901
Size: 5659
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2092 928
Size: 971 193 4495
Fragments arranged by size:
4495 971 193
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3850 3884 1903
Size: 34 1913 34 3678
Fragments arranged by size:
3678 1913 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3551 4761 3551
Size: 1210 4449
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5659
BfrBI ATG'CAT
Cuts at: 2292 2292
Size: 5659
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4270 136
Size: 107 122 71 631 605 2598 1525
Fragments arranged by size:
2598 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5654 5654
Size: 5659
BlpI GC'TnA_GC
Cuts at: 3076 3076
Size: 5659
Bme1580I G_kGCm'C
Cuts at: 1790 2426 2514 3720 4876 1790
Size: 636 88 1206 1156 2573
Fragments arranged by size:
2573 1206 1156 636 88
BmgBI CAC'GTC
Cuts at: 1318 2442 1318
Size: 1124 4535
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3390 4348 442
Size: 416 53 83 399 24 1973 958 1753
Fragments arranged by size:
1973 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5659
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2901 2901
Size: 5659
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2434 2900 4319 1239
Size: 1195 466 1419 2579
Fragments arranged by size:
2579 1419 1195 466
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2540 3784 4649 4947 2540
Size: 1244 865 298 3252
Fragments arranged by size:
3252 1244 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2653 4322 882
Size: 617 1154 1669 2219
Fragments arranged by size:
2219 1669 1154 617
BsaAI yAC'GTr
Cuts at: 493 1204 3450 493
Size: 711 2246 2702
Fragments arranged by size:
2702 2246 711
BsaBI GATnn'nnATC
Cuts at: 5653 5653
Size: 5659
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2898 3466 3848 4483
Size: 53 83 186 936 1365 568 382 635
Cuts at: 4483 275
Size: 1451
Fragments arranged by size:
1451 1365 936 635 568 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4092 4490 4764 4911 5322 4092
Size: 398 274 147 411 4429
Fragments arranged by size:
4429 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5434
Fragments arranged by size:
5434 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2069 2716 2819 1186
Size: 883 647 103 4026
Fragments arranged by size:
4026 883 647 103
BseYI C'CCAG_C
Cuts at: 2044 2119 4862 5358 2044
Size: 75 2743 496 2345
Fragments arranged by size:
2743 2345 496 75
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5086
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3109 3870 4898 1540
Size: 275 186 1108 761 1028 2301
Fragments arranged by size:
2301 1108 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2426 2914 3720 3805 4876 2426
Size: 488 806 85 1071 3209
Fragments arranged by size:
3209 1071 806 488 85
BsmI GAATG_Cn'
Cuts at: 2887 3170 3263 2887
Size: 283 93 5283
Fragments arranged by size:
5283 283 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2426 2514 2914 2978 3720 3805 4876
Size: 399 636 88 400 64 742 85 1071
Cuts at: 4876 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 636 400 399 88 85
64
BspEI T'CCGG_A
Cuts at: 5322 5322
Size: 5659
BspHI T'CATG_A
Cuts at: 2406 3548 5278 2406
Size: 1142 1730 2787
Fragments arranged by size:
2787 1730 1142
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2729 3014 844
Size: 1885 285 3489
Fragments arranged by size:
3489 1885 285
BsrBI CCG'CTC
Cuts at: 1394 1823 3546 1394
Size: 429 1723 3507
Fragments arranged by size:
3507 1723 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4154 4328 65
Size: 1183 2906 174 1396
Fragments arranged by size:
2906 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4303 4490 1700
Size: 110 2493 187 2869
Fragments arranged by size:
2869 2493 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5659
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5659
BssSI C'ACGA_G
Cuts at: 3719 4731 3719
Size: 1012 4647
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5659
BstBI TT'CG_AA
Cuts at: 4463 4463
Size: 5659
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5659
BstXI CCAn_nnnn'nTGG
Cuts at: 2245 2245
Size: 5659
BstYI r'GATC_y
Cuts at: 1972 2682 2989 3356 3741 3758 5210 5296 5308
Size: 710 307 367 385 17 1452 86 12
Cuts at: 5308 5654 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 710 385 367 346 307 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2876 2876
Size: 5659
BtgI C'CryG_G
Cuts at: 513 1065 2004 2766 513
Size: 552 939 762 3406
Fragments arranged by size:
3406 939 762 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4920
Fragments arranged by size:
4920 683 56
BtsI GCAGTG_nn'
Cuts at: 3175 3969 3989 3175
Size: 794 20 4845
Fragments arranged by size:
4845 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5659
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5624
DraI TTT'AAA
Cuts at: 2963 2963
Size: 5659
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4666 809
Size: 507 3350 1802
Fragments arranged by size:
3350 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2199 2373 3106
Size: 54 1425 50 275 18 369 174 733
Cuts at: 3106 3995 5624 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 733 369 275 174 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3106 1537
Size: 275 1294 4090
Fragments arranged by size:
4090 1294 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2096 2537 2918 3589 1353
Size: 743 441 381 671 3423
Fragments arranged by size:
3423 743 671 441 381
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4246 4620 4766 387
Size: 1064 138 2657 374 146 1280
Fragments arranged by size:
2657 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2434 2699 2900 3722 4319 5106 5374
Size: 393 802 265 201 822 597 787 268
Cuts at: 5374 1239
Size: 1524
Fragments arranged by size:
1524 822 802 787 597 393 268 265
201
EcoNI CCTnn'n_nnAGG
Cuts at: 2362 2362
Size: 5659
EcoO109I rG'GnC_Cy
Cuts at: 2511 2511
Size: 5659
EcoRI G'AATT_C
Cuts at: 2968 3346 2968
Size: 378 5281
FspI TGC'GCA
Cuts at: 4165 4165
Size: 5659
HaeII r_GCGC'y
Cuts at: 1678 2901 4806 1678
Size: 1223 1905 2531
Fragments arranged by size:
2531 1905 1223
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4300 4332 4374 4406 1373
Size: 32 135 32 2728 32 42 32 2626
Fragments arranged by size:
2728 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2584 3003 3249 669
Size: 988 281 646 419 246 3079
Fragments arranged by size:
3079 988 646 419 281 246
HindIII A'AGCT_T
Cuts at: 748 2162 748
Size: 1414 4245
HpaI GTT'AAC
Cuts at: 2584 3249 2584
Size: 665 4994
KasI G'GCGC_C
Cuts at: 2897 2897
Size: 5659
KpnI G_GTAC'C
Cuts at: 2984 2984
Size: 5659
MfeI C'AATT_G
Cuts at: 3258 3258
Size: 5659
MluI A'CGCG_T
Cuts at: 5535 5535
Size: 5659
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2250 2725 4773 4957 1305
Size: 945 475 2048 184 2007
Fragments arranged by size:
2048 2007 945 475 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2201 2375 10
Size: 54 1425 343 369 174 3294
Fragments arranged by size:
3294 1425 369 343 174 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2174 2210 2736 2765 3617 3976 4135
Size: 648 1008 36 526 29 852 359 159
Cuts at: 4135 518
Size: 2042
Fragments arranged by size:
2042 1008 852 648 526 359 159 36
29
MspA1I CmG'CkG
Cuts at: 1606 3075 3752 4900 5145 1606
Size: 1469 677 1148 245 2120
Fragments arranged by size:
2120 1469 1148 677 245
NarI GG'CG_CC
Cuts at: 2898 2898
Size: 5659
NcoI C'CATG_G
Cuts at: 513 1065 2004 2766 513
Size: 552 939 762 3406
Fragments arranged by size:
3406 939 762 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5659
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5659
NotI GC'GGCC_GC
Cuts at: 3106 3106
Size: 5659
NsiI A_TGCA'T
Cuts at: 2294 2294
Size: 5659
NspI r_CATG'y
Cuts at: 1132 1229 2215 2659 3017 4562 1132
Size: 97 986 444 358 1545 2229
Fragments arranged by size:
2229 1545 986 444 358 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5659
PciI A'CATG_T
Cuts at: 2211 4558 2211
Size: 2347 3312
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5659
PmeI GTTT'AAAC
Cuts at: 2963 2963
Size: 5659
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3728 3760 5241 5273 3728
Size: 32 1481 32 4114
Fragments arranged by size:
4114 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5659
PsiI TTA'TAA
Cuts at: 3229 3229
Size: 5659
PspOMI G'GGCC_C
Cuts at: 2510 2510
Size: 5659
PstI C_TGCA'G
Cuts at: 830 3011 830
Size: 2181 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5659
SalI G'TCGA_C
Cuts at: 1936 3001 1936
Size: 1065 4594
SbfI CC_TGCA'GG
Cuts at: 3011 3011
Size: 5659
ScaI AGT'ACT
Cuts at: 3907 5619 3907
Size: 1712 3947
SfcI C'TryA_G
Cuts at: 826 1045 2869 3007 4142 4823 5014 826
Size: 219 1824 138 1135 681 191 1471
Fragments arranged by size:
1824 1471 1135 681 219 191 138
SfoI GGC'GCC
Cuts at: 2899 2899
Size: 5659
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5659
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5659
SmaI CCC'GGG
Cuts at: 1872 2516 2986 1872
Size: 644 470 4545
Fragments arranged by size:
4545 644 470
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2555 3763 4664 4926 820
Size: 197 926 612 1208 901 262 1553
Fragments arranged by size:
1553 1208 926 901 612 262 197
SnaBI TAC'GTA
Cuts at: 493 3450 493
Size: 2957 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5659
SphI G_CATG'C
Cuts at: 1132 3017 1132
Size: 1885 3774
SspI AAT'ATT
Cuts at: 5 52 3583 5
Size: 47 3531 2081
Fragments arranged by size:
3531 2081 47
StuI AGG'CCT
Cuts at: 1517 2711 1517
Size: 1194 4465
StyI C'CwwG_G
Cuts at: 513 1065 2004 2766 3098 513
Size: 552 939 762 332 3074
Fragments arranged by size:
3074 939 762 552 332
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2188 3686 3703 3856 4041 4486 325
Size: 1255 608 1498 17 153 185 445 1498
Fragments arranged by size:
1498 1498 1255 608 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2178 2394 3905
Size: 274 80 33 51 1443 201 216 1511
Cuts at: 3905 5617 96
Size: 1712 138
Fragments arranged by size:
1712 1511 1443 274 216 201 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5619 5651 5619
Size: 32 5627
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5659
XbaI T'CTAG_A
Cuts at: 2995 2995
Size: 5659
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5659
XmaI C'CCGG_G
Cuts at: 1870 2514 2984 1870
Size: 644 470 4545
Fragments arranged by size:
4545 644 470
XmnI GAAnn'nnTTC
Cuts at: 3788 3788
Size: 5659
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3467 276
Size: 53 83 186 936 1933 2468
Fragments arranged by size:
2468 1933 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI Bpu10I BsiWI
BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI
NaeI NgoMIV NruI PacI PfoI PmlI PpuMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SrfI SwaI XcmI