Restriction map for
pFN21AE6042
(Circular) (Six-base) MAPSORT of: pFN21AE6042.seq Check: 9861 from: 1 to: 5656
pFN21AE6042 5656 bp
With 182 enzymes: SgfI *
December 8, 2014 13:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2732 2732
Size: 5656
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3466 278
Size: 53 83 186 936 1930 2468
Fragments arranged by size:
2468 1930 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2253 2999 1937
Size: 316 746 4594
Fragments arranged by size:
4594 746 316
Acc65I G'GTAC_C
Cuts at: 2977 2977
Size: 5656
AclI AA'CG_TT
Cuts at: 1563 3783 4156 1563
Size: 2220 373 3063
Fragments arranged by size:
3063 2220 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2579 3719 5103 5371 1632
Size: 947 1140 1384 268 1917
Fragments arranged by size:
1917 1384 1140 947 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5459
AflIII A'CryG_T
Cuts at: 1203 4555 5532 1203
Size: 3352 977 1327
Fragments arranged by size:
3352 1327 977
AgeI A'CCGG_T
Cuts at: 4487 4487
Size: 5656
AhdI GACnn_n'nnGTC
Cuts at: 4385 4385
Size: 5656
AleI CACnn'nnGTG
Cuts at: 1166 2234 2780 1166
Size: 1068 546 4042
Fragments arranged by size:
4042 1068 546
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2408 1184
Size: 1224 4432
AlwNI CAG_nnn'CTG
Cuts at: 2333 3399 4971 2333
Size: 1066 1572 3018
Fragments arranged by size:
3018 1572 1066
ApaI G_GGCC'C
Cuts at: 2905 2905
Size: 5656
ApaLI G'TGCA_C
Cuts at: 3713 4869 3713
Size: 1156 4500
ApoI r'AATT_y
Cuts at: 1493 2664 2965 3191 3343 5452 1493
Size: 1171 301 226 152 2109 1697
Fragments arranged by size:
2109 1697 1171 301 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4210 160
Size: 872 3178 1606
Fragments arranged by size:
3178 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5656
AvaI C'yCGr_G
Cuts at: 1870 1943 2517 2647 2981 1870
Size: 73 574 130 334 4545
Fragments arranged by size:
4545 574 334 130 73
AvrII C'CTAG_G
Cuts at: 2347 2347
Size: 5656
BamHI G'GATC_C
Cuts at: 2986 3353 2986
Size: 367 5289
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2545 2977 4432 618
Size: 325 217 627 758 432 1455 1842
Fragments arranged by size:
1842 1455 758 627 432 325 217
BanII G_rGCy'C
Cuts at: 1391 2654 2905 2975 1391
Size: 1263 251 70 4072
Fragments arranged by size:
4072 1263 251 70
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4685
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2531 2565 3847 3881 1903
Size: 34 594 34 1282 34 3678
Fragments arranged by size:
3678 1282 594 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3548 4758 3548
Size: 1210 4446
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5656
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4267 136
Size: 107 122 71 631 605 2595 1525
Fragments arranged by size:
2595 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2421 5651 2421
Size: 3230 2426
BlpI GC'TnA_GC
Cuts at: 2192 3073 2192
Size: 881 4775
Bme1580I G_kGCm'C
Cuts at: 1790 2241 2905 3717 4873 1790
Size: 451 664 812 1156 2573
Fragments arranged by size:
2573 1156 812 664 451
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5656
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3387 4345 442
Size: 416 53 83 399 24 1970 958 1753
Fragments arranged by size:
1970 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5656
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4316 1239
Size: 3077 2579
Bpu10I CC'TnA_GC
Cuts at: 2323 2808 2323
Size: 485 5171
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3781 4646 4944 3781
Size: 865 298 4493
Fragments arranged by size:
4493 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4319 882
Size: 617 2820 2219
Fragments arranged by size:
2820 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2374 2782 3447 493
Size: 711 1170 408 665 2702
Fragments arranged by size:
2702 1170 711 665 408
BsaBI GATnn'nnATC
Cuts at: 5650 5650
Size: 5656
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3463 3845 4480 275
Size: 53 83 186 936 1930 382 635 1451
Fragments arranged by size:
1930 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4089 4487 4761 4908 5319 4089
Size: 398 274 147 411 4426
Fragments arranged by size:
4426 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2144 2174 1178
Size: 30 165 30 741 30 4660
Fragments arranged by size:
4660 741 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2615 2719 1186
Size: 1429 104 4123
Fragments arranged by size:
4123 1429 104
BseYI C'CCAG_C
Cuts at: 2452 4859 5355 2452
Size: 2407 496 2753
Fragments arranged by size:
2753 2407 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2378 2554 1163
Size: 573 642 176 4265
Fragments arranged by size:
4265 642 573 176
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3106 3867 4895 1540
Size: 275 186 1105 761 1028 2301
Fragments arranged by size:
2301 1105 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3717 3802 4873 3717
Size: 85 1071 4500
Fragments arranged by size:
4500 1071 85
BsmI GAATG_Cn'
Cuts at: 3167 3260 3167
Size: 93 5563
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2241 2654 2905 2975 3717 3802 4873
Size: 399 451 413 251 70 742 85 1071
Cuts at: 4873 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 451 413 399 251 85
70
BspEI T'CCGG_A
Cuts at: 5319 5319
Size: 5656
BspHI T'CATG_A
Cuts at: 2409 3545 5275 2409
Size: 1136 1730 2790
Fragments arranged by size:
2790 1730 1136
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2732 3011 844
Size: 1888 279 3489
Fragments arranged by size:
3489 1888 279
BsrBI CCG'CTC
Cuts at: 1394 1823 3543 1394
Size: 429 1720 3507
Fragments arranged by size:
3507 1720 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4151 4325 65
Size: 1183 2903 174 1396
Fragments arranged by size:
2903 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4300 4487 1700
Size: 110 2490 187 2869
Fragments arranged by size:
2869 2490 187 110
BsrGI T'GTAC_A
Cuts at: 96 2772 96
Size: 2676 2980
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5656
BssSI C'ACGA_G
Cuts at: 3716 4728 3716
Size: 1012 4644
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2424 794
Size: 1630 4026
BstBI TT'CG_AA
Cuts at: 4460 4460
Size: 5656
BstEII G'GTnAC_C
Cuts at: 1184 2944 1184
Size: 1760 3896
BstXI CCAn_nnnn'nTGG
Cuts at: 2131 2248 2131
Size: 117 5539
BstYI r'GATC_y
Cuts at: 1972 2421 2986 3353 3738 3755 5207 5293 5305
Size: 449 565 367 385 17 1452 86 12
Cuts at: 5305 5651 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 565 449 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2207 2879 2935 2207
Size: 672 56 4928
Fragments arranged by size:
4928 672 56
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4165
Fragments arranged by size:
4165 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4917
Fragments arranged by size:
4917 683 56
BtsI GCAGTG_nn'
Cuts at: 2295 3172 3966 3986 2295
Size: 877 794 20 3965
Fragments arranged by size:
3965 877 794 20
ClaI AT'CG_AT
Cuts at: 1554 2466 1554
Size: 912 4744
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5621
DraI TTT'AAA
Cuts at: 2960 2960
Size: 5656
DraIII CAC_nnn'GTG
Cuts at: 2740 2740
Size: 5656
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4663 809
Size: 507 3347 1802
Fragments arranged by size:
3347 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2718 3103 3992
Size: 54 1425 50 275 18 888 385 889
Cuts at: 3992 5621 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 888 385 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3103 1537
Size: 275 1291 4090
Fragments arranged by size:
4090 1291 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2759 3586 1353
Size: 1406 827 3423
Fragments arranged by size:
3423 1406 827
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4243 4617 4763 387
Size: 1064 138 2654 374 146 1280
Fragments arranged by size:
2654 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2579 3719 4316 5103 5371 1239
Size: 393 947 1140 597 787 268 1524
Fragments arranged by size:
1524 1140 947 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2909 2909
Size: 5656
EcoO109I rG'GnC_Cy
Cuts at: 2901 2902 2901
Size: 1 5655
EcoRI G'AATT_C
Cuts at: 2664 2965 3343 2664
Size: 301 378 4977
Fragments arranged by size:
4977 378 301
FspI TGC'GCA
Cuts at: 4162 4162
Size: 5656
HaeII r_GCGC'y
Cuts at: 1678 4803 1678
Size: 3125 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2448 2480 4297 4329 4371
Size: 32 135 32 876 32 1817 32 42
Cuts at: 4371 4403 1373
Size: 32 2626
Fragments arranged by size:
2626 1817 876 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2254 3000 3246 669
Size: 988 281 316 746 246 3079
Fragments arranged by size:
3079 988 746 316 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5656
HpaI GTT'AAC
Cuts at: 3246 3246
Size: 5656
KpnI G_GTAC'C
Cuts at: 2981 2981
Size: 5656
MfeI C'AATT_G
Cuts at: 3255 3255
Size: 5656
MluI A'CGCG_T
Cuts at: 5532 5532
Size: 5656
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4770 4954 1305
Size: 3465 184 2007
Fragments arranged by size:
3465 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2720 10
Size: 54 1425 343 888 2946
Fragments arranged by size:
2946 1425 888 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2177 2234 2780 2852 3614 3973 4132
Size: 648 1011 57 546 72 762 359 159
Cuts at: 4132 518
Size: 2042
Fragments arranged by size:
2042 1011 762 648 546 359 159 72
57
MspA1I CmG'CkG
Cuts at: 1606 3072 3749 4897 5142 1606
Size: 1466 677 1148 245 2120
Fragments arranged by size:
2120 1466 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4165
Fragments arranged by size:
4165 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5656
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5656
NotI GC'GGCC_GC
Cuts at: 3103 3103
Size: 5656
NspI r_CATG'y
Cuts at: 1132 1229 3014 4559 1132
Size: 97 1785 1545 2229
Fragments arranged by size:
2229 1785 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5656
PciI A'CATG_T
Cuts at: 4555 4555
Size: 5656
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5656
PmeI GTTT'AAAC
Cuts at: 2960 2960
Size: 5656
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3725 3757 5238 5270 3725
Size: 32 1481 32 4111
Fragments arranged by size:
4111 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2356 1613
Size: 743 4913
PsiI TTA'TAA
Cuts at: 3226 3226
Size: 5656
PspOMI G'GGCC_C
Cuts at: 2901 2901
Size: 5656
PstI C_TGCA'G
Cuts at: 830 3008 830
Size: 2178 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5656
SalI G'TCGA_C
Cuts at: 1936 2252 2998 1936
Size: 316 746 4594
Fragments arranged by size:
4594 746 316
SbfI CC_TGCA'GG
Cuts at: 3008 3008
Size: 5656
ScaI AGT'ACT
Cuts at: 3904 5616 3904
Size: 1712 3944
SexAI A'CCwGG_T
Cuts at: 2256 2256
Size: 5656
SfcI C'TryA_G
Cuts at: 826 1045 2906 3004 4139 4820 5011 826
Size: 219 1861 98 1135 681 191 1471
Fragments arranged by size:
1861 1471 1135 681 219 191 98
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5656
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5656
SmaI CCC'GGG
Cuts at: 1872 2983 1872
Size: 1111 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3760 4661 4923 820
Size: 197 926 1817 901 262 1553
Fragments arranged by size:
1817 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2374 3447 493
Size: 1881 1073 2702
Fragments arranged by size:
2702 1881 1073
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5656
SphI G_CATG'C
Cuts at: 1132 3014 1132
Size: 1882 3774
SspI AAT'ATT
Cuts at: 5 52 3580 5
Size: 47 3528 2081
Fragments arranged by size:
3528 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5656
StyI C'CwwG_G
Cuts at: 513 1065 2004 2347 3095 513
Size: 552 939 343 748 3074
Fragments arranged by size:
3074 939 748 552 343
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3683 3700 3853 4038 4483 325
Size: 1255 2103 17 153 185 445 1498
Fragments arranged by size:
2103 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 2772 3902
Size: 274 80 33 51 1443 204 591 1130
Cuts at: 3902 5614 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1130 591 274 204 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2144 2176 5616 5648 2144
Size: 32 3440 32 2152
Fragments arranged by size:
3440 2152 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5656
XbaI T'CTAG_A
Cuts at: 2992 2992
Size: 5656
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5656
XmaI C'CCGG_G
Cuts at: 1870 2981 1870
Size: 1111 4545
XmnI GAAnn'nnTTC
Cuts at: 3785 3785
Size: 5656
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3464 276
Size: 53 83 186 936 1930 2468
Fragments arranged by size:
2468 1930 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbeI BbvCI BfrBI BplI
BsiWI BsmBI BstZ17I EcoICRI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SfiI SfoI SrfI SwaI XcmI