Restriction map for
pFN21AE6036
(Circular) (Six-base) MAPSORT of: pFN21AE6036.seq Check: 3117 from: 1 to: 5656
pFN21AE6036 5656 bp
With 182 enzymes: SgfI *
December 8, 2014 13:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3466 278
Size: 53 83 186 936 1930 2468
Fragments arranged by size:
2468 1930 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2999 1937
Size: 1062 4594
Acc65I G'GTAC_C
Cuts at: 2977 2977
Size: 5656
AclI AA'CG_TT
Cuts at: 1563 2274 3783 4156 1563
Size: 711 1509 373 3063
Fragments arranged by size:
3063 1509 711 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3719 5103 5371 1632
Size: 2087 1384 268 1917
Fragments arranged by size:
2087 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5459
AflIII A'CryG_T
Cuts at: 1203 4555 5532 1203
Size: 3352 977 1327
Fragments arranged by size:
3352 1327 977
AgeI A'CCGG_T
Cuts at: 4487 4487
Size: 5656
AhdI GACnn_n'nnGTC
Cuts at: 4385 4385
Size: 5656
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5656
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5656
AlwNI CAG_nnn'CTG
Cuts at: 3399 4971 3399
Size: 1572 4084
ApaLI G'TGCA_C
Cuts at: 3713 4869 3713
Size: 1156 4500
ApoI r'AATT_y
Cuts at: 1493 2923 2965 3191 3343 5452 1493
Size: 1430 42 226 152 2109 1697
Fragments arranged by size:
2109 1697 1430 226 152 42
AseI AT'TA_AT
Cuts at: 160 1032 4210 160
Size: 872 3178 1606
Fragments arranged by size:
3178 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5656
AvaI C'yCGr_G
Cuts at: 1870 1943 2981 1870
Size: 73 1038 4545
Fragments arranged by size:
4545 1038 73
BamHI G'GATC_C
Cuts at: 2638 2986 3353 2638
Size: 348 367 4941
Fragments arranged by size:
4941 367 348
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2857 2977 4432 618
Size: 325 217 627 1070 120 1455 1842
Fragments arranged by size:
1842 1455 1070 627 325 217 120
BanII G_rGCy'C
Cuts at: 1391 2975 1391
Size: 1584 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2337 928
Size: 971 438 4247
Fragments arranged by size:
4247 971 438
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3847 3881 1903
Size: 34 1910 34 3678
Fragments arranged by size:
3678 1910 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3548 4758 3548
Size: 1210 4446
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5656
BfrBI ATG'CAT
Cuts at: 2947 2947
Size: 5656
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2702 4267 136
Size: 107 122 71 631 605 1030 1565 1525
Fragments arranged by size:
1565 1525 1030 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5651 5651
Size: 5656
BlpI GC'TnA_GC
Cuts at: 2706 3073 2706
Size: 367 5289
Bme1580I G_kGCm'C
Cuts at: 1790 2693 2722 3717 4873 1790
Size: 903 29 995 1156 2573
Fragments arranged by size:
2573 1156 995 903 29
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5656
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2451 3387 4345
Size: 416 53 83 399 24 1034 936 958
Cuts at: 4345 442
Size: 1753
Fragments arranged by size:
1753 1034 958 936 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5656
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4316 1239
Size: 3077 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2483 3781 4646 4944 2483
Size: 1298 865 298 3195
Fragments arranged by size:
3195 1298 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2231 4319 882
Size: 617 732 2088 2219
Fragments arranged by size:
2219 2088 732 617
BsaAI yAC'GTr
Cuts at: 493 1204 3447 493
Size: 711 2243 2702
Fragments arranged by size:
2702 2243 711
BsaBI GATnn'nnATC
Cuts at: 2093 5650 2093
Size: 3557 2099
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3463 3845 4480 275
Size: 53 83 186 936 1930 382 635 1451
Fragments arranged by size:
1930 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4089 4487 4761 4908 5319 4089
Size: 398 274 147 411 4426
Fragments arranged by size:
4426 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2105 2135 1178
Size: 30 165 30 702 30 4699
Fragments arranged by size:
4699 702 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5656
BseYI C'CCAG_C
Cuts at: 4859 5355 4859
Size: 496 5160
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5083
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3106 3867 4895 1540
Size: 275 186 1105 761 1028 2301
Fragments arranged by size:
2301 1105 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3717 3802 4873 3717
Size: 85 1071 4500
Fragments arranged by size:
4500 1071 85
BsmI GAATG_Cn'
Cuts at: 2947 3167 3260 2947
Size: 220 93 5343
Fragments arranged by size:
5343 220 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2693 2722 2975 3717 3802 4873 1391
Size: 399 903 29 253 742 85 1071 2174
Fragments arranged by size:
2174 1071 903 742 399 253 85 29
BspEI T'CCGG_A
Cuts at: 5319 5319
Size: 5656
BspHI T'CATG_A
Cuts at: 3545 5275 3545
Size: 1730 3926
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2859 3011 844
Size: 2015 152 3489
Fragments arranged by size:
3489 2015 152
BsrBI CCG'CTC
Cuts at: 1394 1823 3543 1394
Size: 429 1720 3507
Fragments arranged by size:
3507 1720 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4151 4325 65
Size: 1183 2903 174 1396
Fragments arranged by size:
2903 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4300 4487 1700
Size: 110 2490 187 2869
Fragments arranged by size:
2869 2490 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5656
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5656
BssSI C'ACGA_G
Cuts at: 3716 4728 3716
Size: 1012 4644
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5656
BstBI TT'CG_AA
Cuts at: 4460 4460
Size: 5656
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5656
BstYI r'GATC_y
Cuts at: 1972 2088 2638 2904 2986 3353 3738 3755 5207
Size: 116 550 266 82 367 385 17 1452
Cuts at: 5207 5293 5305 5651 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 550 385 367 346 266 116
86 82 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2772 513
Size: 552 1707 3397
Fragments arranged by size:
3397 1707 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2780 487
Size: 683 56 1554 3363
Fragments arranged by size:
3363 1554 683 56
BtsI GCAGTG_nn'
Cuts at: 3172 3966 3986 3172
Size: 794 20 4842
Fragments arranged by size:
4842 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5656
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5621
DraI TTT'AAA
Cuts at: 2960 2960
Size: 5656
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4663 809
Size: 507 3347 1802
Fragments arranged by size:
3347 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2382 2838 3103
Size: 54 1425 50 275 18 552 456 265
Cuts at: 3103 3992 5621 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 552 456 275 265 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3103 1537
Size: 275 1291 4090
Fragments arranged by size:
4090 1291 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3586 1353
Size: 2233 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2555 4243 4617 4763 387
Size: 1064 138 966 1688 374 146 1280
Fragments arranged by size:
1688 1280 1064 966 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3719 4316 5103 5371 1239
Size: 393 2087 597 787 268 1524
Fragments arranged by size:
2087 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2552 2552
Size: 5656
EcoRI G'AATT_C
Cuts at: 2965 3343 2965
Size: 378 5278
FspI TGC'GCA
Cuts at: 4162 4162
Size: 5656
HaeII r_GCGC'y
Cuts at: 1678 4803 1678
Size: 3125 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4297 4329 4371 4403 1373
Size: 32 135 32 2725 32 42 32 2626
Fragments arranged by size:
2725 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2371 3000 3246 669
Size: 988 281 433 629 246 3079
Fragments arranged by size:
3079 988 629 433 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5656
HpaI GTT'AAC
Cuts at: 3246 3246
Size: 5656
KpnI G_GTAC'C
Cuts at: 2981 2981
Size: 5656
MfeI C'AATT_G
Cuts at: 3255 3255
Size: 5656
MluI A'CGCG_T
Cuts at: 5532 5532
Size: 5656
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4770 4954 1305
Size: 3465 184 2007
Fragments arranged by size:
3465 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2384 2840 10
Size: 54 1425 343 552 456 2826
Fragments arranged by size:
2826 1425 552 456 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2252 2771 3614 3973 4132 518
Size: 648 1086 519 843 359 159 2042
Fragments arranged by size:
2042 1086 843 648 519 359 159
MspA1I CmG'CkG
Cuts at: 1606 2598 3072 3749 4897 5142 1606
Size: 992 474 677 1148 245 2120
Fragments arranged by size:
2120 1148 992 677 474 245
NcoI C'CATG_G
Cuts at: 513 1065 2772 513
Size: 552 1707 3397
Fragments arranged by size:
3397 1707 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5656
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5656
NotI GC'GGCC_GC
Cuts at: 3103 3103
Size: 5656
NsiI A_TGCA'T
Cuts at: 2949 2949
Size: 5656
NspI r_CATG'y
Cuts at: 1132 1229 2188 2813 3014 4559 1132
Size: 97 959 625 201 1545 2229
Fragments arranged by size:
2229 1545 959 625 201 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5656
PciI A'CATG_T
Cuts at: 4555 4555
Size: 5656
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5656
PmeI GTTT'AAAC
Cuts at: 2960 2960
Size: 5656
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3725 3757 5238 5270 3725
Size: 32 1481 32 4111
Fragments arranged by size:
4111 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2223 1613
Size: 610 5046
PsiI TTA'TAA
Cuts at: 3226 3226
Size: 5656
PstI C_TGCA'G
Cuts at: 830 3008 830
Size: 2178 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5656
PvuII CAG'CTG
Cuts at: 2598 2598
Size: 5656
SalI G'TCGA_C
Cuts at: 1936 2998 1936
Size: 1062 4594
SbfI CC_TGCA'GG
Cuts at: 3008 3008
Size: 5656
ScaI AGT'ACT
Cuts at: 3904 5616 3904
Size: 1712 3944
SfcI C'TryA_G
Cuts at: 826 1045 3004 4139 4820 5011 826
Size: 219 1959 1135 681 191 1471
Fragments arranged by size:
1959 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5656
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5656
SmaI CCC'GGG
Cuts at: 1872 2983 1872
Size: 1111 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2498 3760 4661 4923 820
Size: 197 926 555 1262 901 262 1553
Fragments arranged by size:
1553 1262 926 901 555 262 197
SnaBI TAC'GTA
Cuts at: 493 3447 493
Size: 2954 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5656
SphI G_CATG'C
Cuts at: 1132 2813 3014 1132
Size: 1681 201 3774
Fragments arranged by size:
3774 1681 201
SspI AAT'ATT
Cuts at: 5 52 3580 5
Size: 47 3528 2081
Fragments arranged by size:
3528 2081 47
StuI AGG'CCT
Cuts at: 1517 2075 2297 1517
Size: 558 222 4876
Fragments arranged by size:
4876 558 222
StyI C'CwwG_G
Cuts at: 513 1065 2772 3095 513
Size: 552 1707 323 3074
Fragments arranged by size:
3074 1707 552 323
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3683 3700 3853 4038 4483 325
Size: 1255 2103 17 153 185 445 1498
Fragments arranged by size:
2103 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3902 5614 96
Size: 274 80 33 51 1443 1925 1712 138
Fragments arranged by size:
1925 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5616 5648 5616
Size: 32 5624
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5656
XbaI T'CTAG_A
Cuts at: 2992 2992
Size: 5656
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2125 2125
Size: 5656
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5656
XmaI C'CCGG_G
Cuts at: 1870 2981 1870
Size: 1111 4545
XmnI GAAnn'nnTTC
Cuts at: 3785 3785
Size: 5656
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3464 276
Size: 53 83 186 936 1930 2468
Fragments arranged by size:
2468 1930 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI