Restriction map for
pFN21AE6030
(Circular) (Six-base) MAPSORT of: pFN21AE6030.seq Check: 335 from: 1 to: 5653
pFN21AE6030 5653 bp
With 182 enzymes: SgfI *
December 8, 2014 13:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3463 278
Size: 53 83 186 936 1927 2468
Fragments arranged by size:
2468 1927 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2996 1937
Size: 1059 4594
Acc65I G'GTAC_C
Cuts at: 2974 2974
Size: 5653
AclI AA'CG_TT
Cuts at: 1563 3780 4153 1563
Size: 2217 373 3063
Fragments arranged by size:
3063 2217 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2921 3716 5100 5368 1632
Size: 1289 795 1384 268 1917
Fragments arranged by size:
1917 1384 1289 795 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5456
AflIII A'CryG_T
Cuts at: 1203 2729 4552 5529 1203
Size: 1526 1823 977 1327
Fragments arranged by size:
1823 1526 1327 977
AgeI A'CCGG_T
Cuts at: 4484 4484
Size: 5653
AhdI GACnn_n'nnGTC
Cuts at: 4382 4382
Size: 5653
AleI CACnn'nnGTG
Cuts at: 1166 2288 2750 1166
Size: 1122 462 4069
Fragments arranged by size:
4069 1122 462
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5653
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2154 2186 2154
Size: 32 5621
AlwNI CAG_nnn'CTG
Cuts at: 3396 4968 3396
Size: 1572 4081
ApaLI G'TGCA_C
Cuts at: 2197 3710 4866 2197
Size: 1513 1156 2984
Fragments arranged by size:
2984 1513 1156
ApoI r'AATT_y
Cuts at: 1493 2036 2933 2962 3188 3340 5449 1493
Size: 543 897 29 226 152 2109 1697
Fragments arranged by size:
2109 1697 897 543 226 152 29
AseI AT'TA_AT
Cuts at: 160 1032 4207 160
Size: 872 3175 1606
Fragments arranged by size:
3175 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5653
AvaI C'yCGr_G
Cuts at: 1870 1943 2978 1870
Size: 73 1035 4545
Fragments arranged by size:
4545 1035 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2168 2201 2718 2751 2168
Size: 33 517 33 5070
Fragments arranged by size:
5070 517 33 33
BamHI G'GATC_C
Cuts at: 2983 3350 2983
Size: 367 5286
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2974 4429 618
Size: 325 217 627 1187 1455 1842
Fragments arranged by size:
1842 1455 1187 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2420 2972 1391
Size: 1029 552 4072
Fragments arranged by size:
4072 1029 552
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2659 2842 928
Size: 971 760 183 3739
Fragments arranged by size:
3739 971 760 183
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3844 3878 1903
Size: 34 50 34 1823 34 3678
Fragments arranged by size:
3678 1823 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2746 3545 4755 2746
Size: 799 1210 3644
Fragments arranged by size:
3644 1210 799
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5653
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2831 4264 136
Size: 107 122 71 631 605 1159 1433 1525
Fragments arranged by size:
1525 1433 1159 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5648 5648
Size: 5653
BlpI GC'TnA_GC
Cuts at: 3070 3070
Size: 5653
Bme1580I G_kGCm'C
Cuts at: 1790 2201 2434 3714 4870 1790
Size: 411 233 1280 1156 2573
Fragments arranged by size:
2573 1280 1156 411 233
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5653
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3384 4342 442
Size: 416 53 83 399 24 1967 958 1753
Fragments arranged by size:
1967 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5653
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4313 1239
Size: 3074 2579
Bpu10I CC'TnA_GC
Cuts at: 2864 2864
Size: 5653
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2707 2774 3778 4643 4941 2707
Size: 67 1004 865 298 3419
Fragments arranged by size:
3419 1004 865 298 67
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4316 882
Size: 617 2817 2219
Fragments arranged by size:
2817 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2730 3444 493
Size: 711 1526 714 2702
Fragments arranged by size:
2702 1526 714 711
BsaBI GATnn'nnATC
Cuts at: 5647 5647
Size: 5653
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2823 3460 3842 4477
Size: 53 83 186 936 1290 637 382 635
Cuts at: 4477 275
Size: 1451
Fragments arranged by size:
1451 1290 936 637 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4086 4484 4758 4905 5316 4086
Size: 398 274 147 411 4423
Fragments arranged by size:
4423 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2154 2184 1178
Size: 30 165 30 751 30 4647
Fragments arranged by size:
4647 751 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5653
BseYI C'CCAG_C
Cuts at: 2067 2125 4856 5352 2067
Size: 58 2731 496 2368
Fragments arranged by size:
2731 2368 496 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5080
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3103 3864 4892 1540
Size: 275 186 1102 761 1028 2301
Fragments arranged by size:
2301 1102 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2201 2412 3714 3799 4870 2201
Size: 211 1302 85 1071 2984
Fragments arranged by size:
2984 1302 1071 211 85
BsmI GAATG_Cn'
Cuts at: 2911 3164 3257 2911
Size: 253 93 5307
Fragments arranged by size:
5307 253 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2215 2215
Size: 5653
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2201 2412 2420 2434 2972 3714 3799
Size: 399 411 211 8 14 538 742 85
Cuts at: 3799 4870 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 538 411 399 211 85
14 8
BspEI T'CCGG_A
Cuts at: 5316 5316
Size: 5653
BspHI T'CATG_A
Cuts at: 2412 3542 5272 2412
Size: 1130 1730 2793
Fragments arranged by size:
2793 1730 1130
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3008 844
Size: 2164 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3540 1394
Size: 429 1717 3507
Fragments arranged by size:
3507 1717 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2120 4148 4322 65
Size: 1183 872 2028 174 1396
Fragments arranged by size:
2028 1396 1183 872 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2709 4297 4484 1700
Size: 110 899 1588 187 2869
Fragments arranged by size:
2869 1588 899 187 110
BsrGI T'GTAC_A
Cuts at: 96 2814 96
Size: 2718 2935
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5653
BssSI C'ACGA_G
Cuts at: 3713 4725 3713
Size: 1012 4641
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2077 794
Size: 1283 4370
BstBI TT'CG_AA
Cuts at: 4457 4457
Size: 5653
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5653
BstYI r'GATC_y
Cuts at: 1972 2983 3350 3735 3752 5204 5290 5302 5648
Size: 1011 367 385 17 1452 86 12 346
Cuts at: 5648 1972
Size: 1977
Fragments arranged by size:
1977 1452 1011 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2885 2885
Size: 5653
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5101
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4914
Fragments arranged by size:
4914 683 56
BtsI GCAGTG_nn'
Cuts at: 2393 3169 3963 3983 2393
Size: 776 794 20 4063
Fragments arranged by size:
4063 794 776 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5653
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5618
DraI TTT'AAA
Cuts at: 2957 2957
Size: 5653
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4660 809
Size: 507 3344 1802
Fragments arranged by size:
3344 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2145 2379 2832
Size: 54 1425 50 275 18 315 234 453
Cuts at: 2832 3100 3989 5618 8
Size: 268 889 1629 43
Fragments arranged by size:
1629 1425 889 453 315 275 268 234
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3100 1537
Size: 275 1288 4090
Fragments arranged by size:
4090 1288 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2195 3583 1353
Size: 842 1388 3423
Fragments arranged by size:
3423 1388 842
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4240 4614 4760 387
Size: 1064 138 2651 374 146 1280
Fragments arranged by size:
2651 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2921 3716 4313 5100 5368 1239
Size: 393 1289 795 597 787 268 1524
Fragments arranged by size:
1524 1289 795 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2800 2800
Size: 5653
EcoRI G'AATT_C
Cuts at: 2962 3340 2962
Size: 378 5275
FspI TGC'GCA
Cuts at: 4159 4159
Size: 5653
HaeII r_GCGC'y
Cuts at: 1678 4800 1678
Size: 3122 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4294 4326 4368 4400 1373
Size: 32 135 32 2722 32 42 32 2626
Fragments arranged by size:
2722 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2997 3243 669
Size: 988 281 1059 246 3079
Fragments arranged by size:
3079 1059 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5653
HpaI GTT'AAC
Cuts at: 3243 3243
Size: 5653
KpnI G_GTAC'C
Cuts at: 2978 2978
Size: 5653
MfeI C'AATT_G
Cuts at: 3252 3252
Size: 5653
MluI A'CGCG_T
Cuts at: 5529 5529
Size: 5653
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4767 4951 1305
Size: 3462 184 2007
Fragments arranged by size:
3462 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2147 2381 2834 10
Size: 54 1425 343 315 234 453 2829
Fragments arranged by size:
2829 1425 453 343 315 234 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2180 2288 2474 2603 2750 3611 3970
Size: 648 1014 108 186 129 147 861 359
Cuts at: 3970 4129 518
Size: 159 2042
Fragments arranged by size:
2042 1014 861 648 359 186 159 147
129 108
MspA1I CmG'CkG
Cuts at: 1606 3069 3746 4894 5139 1606
Size: 1463 677 1148 245 2120
Fragments arranged by size:
2120 1463 1148 677 245
NaeI GCC'GGC
Cuts at: 2711 2711
Size: 5653
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5101
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5653
NgoMIV G'CCGG_C
Cuts at: 2709 2709
Size: 5653
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5653
NotI GC'GGCC_GC
Cuts at: 3100 3100
Size: 5653
NspI r_CATG'y
Cuts at: 1132 1229 2251 3011 4556 1132
Size: 97 1022 760 1545 2229
Fragments arranged by size:
2229 1545 1022 760 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5653
PciI A'CATG_T
Cuts at: 4552 4552
Size: 5653
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2154 2440 1108
Size: 1046 286 4321
Fragments arranged by size:
4321 1046 286
PmeI GTTT'AAAC
Cuts at: 2957 2957
Size: 5653
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2154 2186 2207 2239 3722 3754 5235 5267 2154
Size: 32 21 32 1483 32 1481 32 2540
Fragments arranged by size:
2540 1483 1481 32 32 32 32 21
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5653
PsiI TTA'TAA
Cuts at: 3223 3223
Size: 5653
PstI C_TGCA'G
Cuts at: 830 3005 830
Size: 2175 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5653
SalI G'TCGA_C
Cuts at: 1936 2995 1936
Size: 1059 4594
SbfI CC_TGCA'GG
Cuts at: 3005 3005
Size: 5653
ScaI AGT'ACT
Cuts at: 3901 5613 3901
Size: 1712 3941
SfcI C'TryA_G
Cuts at: 826 1045 2020 2764 3001 4136 4817 5008 826
Size: 219 975 744 237 1135 681 191 1471
Fragments arranged by size:
1471 1135 975 744 681 237 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5653
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5653
SmaI CCC'GGG
Cuts at: 1872 2980 1872
Size: 1108 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2686 2789 3757 4658 4920 820
Size: 197 926 743 103 968 901 262 1553
Fragments arranged by size:
1553 968 926 901 743 262 197 103
SnaBI TAC'GTA
Cuts at: 493 3444 493
Size: 2951 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5653
SphI G_CATG'C
Cuts at: 1132 2251 3011 1132
Size: 1119 760 3774
Fragments arranged by size:
3774 1119 760
SspI AAT'ATT
Cuts at: 5 52 3577 5
Size: 47 3525 2081
Fragments arranged by size:
3525 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5653
StyI C'CwwG_G
Cuts at: 513 1065 2796 3092 513
Size: 552 1731 296 3074
Fragments arranged by size:
3074 1731 552 296
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2194 2285 2406 2591 3680 3697 3850
Size: 1255 614 91 121 185 1089 17 153
Cuts at: 3850 4035 4480 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1089 614 445 185 185 153
121 91 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2814 3899 5611
Size: 274 80 33 51 1443 837 1085 1712
Cuts at: 5611 96
Size: 138
Fragments arranged by size:
1712 1443 1085 837 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5613 5645 5613
Size: 32 5621
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5653
XbaI T'CTAG_A
Cuts at: 2989 2989
Size: 5653
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2287 2287
Size: 5653
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5653
XmaI C'CCGG_G
Cuts at: 1870 2978 1870
Size: 1108 4545
XmnI GAAnn'nnTTC
Cuts at: 3782 3782
Size: 5653
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3461 276
Size: 53 83 186 936 1927 2468
Fragments arranged by size:
2468 1927 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbeI BbvCI BfrBI
BplI BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI