Restriction map for
pFN21AE6024
(Circular) (Six-base) MAPSORT of: pFN21AE6024.seq Check: 5770 from: 1 to: 5650
pFN21AE6024 5650 bp
With 182 enzymes: SgfI *
December 8, 2014 13:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3460 278
Size: 53 83 186 936 1924 2468
Fragments arranged by size:
2468 1924 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2993 1937
Size: 1056 4594
Acc65I G'GTAC_C
Cuts at: 2971 2971
Size: 5650
AclI AA'CG_TT
Cuts at: 1563 3777 4150 1563
Size: 2214 373 3063
Fragments arranged by size:
3063 2214 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2060 2668 2930 3713 5097 5365 1632
Size: 428 608 262 783 1384 268 1917
Fragments arranged by size:
1917 1384 783 608 428 268 262
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5453
AflIII A'CryG_T
Cuts at: 1203 4549 5526 1203
Size: 3346 977 1327
Fragments arranged by size:
3346 1327 977
AgeI A'CCGG_T
Cuts at: 4481 4481
Size: 5650
AhdI GACnn_n'nnGTC
Cuts at: 4379 4379
Size: 5650
AleI CACnn'nnGTG
Cuts at: 1166 2585 2846 1166
Size: 1419 261 3970
Fragments arranged by size:
3970 1419 261
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2840 1184
Size: 1656 3994
AlwNI CAG_nnn'CTG
Cuts at: 2615 3393 4965 2615
Size: 778 1572 3300
Fragments arranged by size:
3300 1572 778
ApaLI G'TGCA_C
Cuts at: 3707 4863 3707
Size: 1156 4494
ApoI r'AATT_y
Cuts at: 1493 2036 2959 3185 3337 5446 1493
Size: 543 923 226 152 2109 1697
Fragments arranged by size:
2109 1697 923 543 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4204 160
Size: 872 3172 1606
Fragments arranged by size:
3172 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5650
AvaI C'yCGr_G
Cuts at: 1870 1943 2975 1870
Size: 73 1032 4545
Fragments arranged by size:
4545 1032 73
BamHI G'GATC_C
Cuts at: 2654 2980 3347 2654
Size: 326 367 4957
Fragments arranged by size:
4957 367 326
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2971 4426 618
Size: 325 217 627 1184 1455 1842
Fragments arranged by size:
1842 1455 1184 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2939 2969 1391
Size: 1548 30 4072
Fragments arranged by size:
4072 1548 30
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4679
BbvCI CC'TCA_GC
Cuts at: 2700 2700
Size: 5650
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3841 3875 1903
Size: 34 1904 34 3678
Fragments arranged by size:
3678 1904 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3542 4752 3542
Size: 1210 4440
BclI T'GATC_A
Cuts at: 1548 2262 1548
Size: 714 4936
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2360 2438 4261
Size: 107 122 71 631 605 688 78 1823
Cuts at: 4261 136
Size: 1525
Fragments arranged by size:
1823 1525 688 631 605 122 107 78
71
BglII A'GATC_T
Cuts at: 5645 5645
Size: 5650
BlpI GC'TnA_GC
Cuts at: 3067 3067
Size: 5650
Bme1580I G_kGCm'C
Cuts at: 1790 2244 2434 3711 4867 1790
Size: 454 190 1277 1156 2573
Fragments arranged by size:
2573 1277 1156 454 190
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5650
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2399 3381 4339
Size: 416 53 83 399 24 982 982 958
Cuts at: 4339 442
Size: 1753
Fragments arranged by size:
1753 982 982 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5650
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2234 2618 4310 1239
Size: 995 384 1692 2579
Fragments arranged by size:
2579 1692 995 384
Bpu10I CC'TnA_GC
Cuts at: 2700 2700
Size: 5650
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2866 3775 4640 4938 2866
Size: 909 865 298 3578
Fragments arranged by size:
3578 909 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4313 882
Size: 617 2814 2219
Fragments arranged by size:
2814 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2377 2781 3441 493
Size: 711 1173 404 660 2702
Fragments arranged by size:
2702 1173 711 660 404
BsaBI GATnn'nnATC
Cuts at: 5644 5644
Size: 5650
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3457 3839 4474 275
Size: 53 83 186 936 1924 382 635 1451
Fragments arranged by size:
1924 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4083 4481 4755 4902 5313 4083
Size: 398 274 147 411 4420
Fragments arranged by size:
4420 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2076 2106 1178
Size: 30 165 30 673 30 4722
Fragments arranged by size:
4722 673 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5650
BseYI C'CCAG_C
Cuts at: 2251 2433 4853 5349 2251
Size: 182 2420 496 2552
Fragments arranged by size:
2552 2420 496 182
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2151 2889 1163
Size: 573 415 738 3924
Fragments arranged by size:
3924 738 573 415
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3100 3861 4889 1540
Size: 275 186 1099 761 1028 2301
Fragments arranged by size:
2301 1099 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3711 3796 4867 3711
Size: 85 1071 4494
Fragments arranged by size:
4494 1071 85
BsmI GAATG_Cn'
Cuts at: 2717 3161 3254 2717
Size: 444 93 5113
Fragments arranged by size:
5113 444 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2244 2434 2939 2969 3711 3796 4867
Size: 399 454 190 505 30 742 85 1071
Cuts at: 4867 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 505 454 399 190 85
30
BspEI T'CCGG_A
Cuts at: 5313 5313
Size: 5650
BspHI T'CATG_A
Cuts at: 3539 5269 3539
Size: 1730 3920
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3005 844
Size: 2161 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3537 1394
Size: 429 1714 3507
Fragments arranged by size:
3507 1714 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2137 4145 4319 65
Size: 1183 889 2008 174 1396
Fragments arranged by size:
2008 1396 1183 889 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4294 4481 1700
Size: 110 2484 187 2869
Fragments arranged by size:
2869 2484 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5650
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5650
BssSI C'ACGA_G
Cuts at: 2045 3710 4722 2045
Size: 1665 1012 2973
Fragments arranged by size:
2973 1665 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5650
BstBI TT'CG_AA
Cuts at: 4454 4454
Size: 5650
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5650
BstXI CCAn_nnnn'nTGG
Cuts at: 2113 2251 2113
Size: 138 5512
BstYI r'GATC_y
Cuts at: 1972 2654 2980 3347 3732 3749 5201 5287 5299
Size: 682 326 367 385 17 1452 86 12
Cuts at: 5299 5645 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 682 385 367 346 326 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2358 513
Size: 552 1293 3805
Fragments arranged by size:
3805 1293 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4911
Fragments arranged by size:
4911 683 56
BtsI GCAGTG_nn'
Cuts at: 2766 3166 3960 3980 2766
Size: 400 794 20 4436
Fragments arranged by size:
4436 794 400 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5650
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5615
DraI TTT'AAA
Cuts at: 2954 2954
Size: 5650
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4657 809
Size: 507 3341 1802
Fragments arranged by size:
3341 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2352 2379 2472
Size: 54 1425 50 275 18 522 27 93
Cuts at: 2472 3097 3986 5615 8
Size: 625 889 1629 43
Fragments arranged by size:
1629 1425 889 625 522 275 93 54
50 43 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 3097 1537
Size: 275 1285 4090
Fragments arranged by size:
4090 1285 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2762 2812 2842 3580 1353
Size: 1409 50 30 738 3423
Fragments arranged by size:
3423 1409 738 50 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2722 4237 4611 4757 387
Size: 1064 138 1133 1515 374 146 1280
Fragments arranged by size:
1515 1280 1133 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2060 2234 2618 2668 2930 3713 4310
Size: 393 428 174 384 50 262 783 597
Cuts at: 4310 5097 5365 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 783 597 428 393 384 268
262 174 50
EcoO109I rG'GnC_Cy
Cuts at: 2256 2928 2256
Size: 672 4978
EcoRI G'AATT_C
Cuts at: 2959 3337 2959
Size: 378 5272
FspI TGC'GCA
Cuts at: 4156 4156
Size: 5650
HaeII r_GCGC'y
Cuts at: 1678 4797 1678
Size: 3119 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2646 2678 4291 4323 4365
Size: 32 135 32 1074 32 1613 32 42
Cuts at: 4365 4397 1373
Size: 32 2626
Fragments arranged by size:
2626 1613 1074 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2994 3240 669
Size: 988 281 1056 246 3079
Fragments arranged by size:
3079 1056 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5650
HpaI GTT'AAC
Cuts at: 3240 3240
Size: 5650
KpnI G_GTAC'C
Cuts at: 2975 2975
Size: 5650
MfeI C'AATT_G
Cuts at: 2025 3249 2025
Size: 1224 4426
MluI A'CGCG_T
Cuts at: 5526 5526
Size: 5650
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2812 4764 4948 1305
Size: 1507 1952 184 2007
Fragments arranged by size:
2007 1952 1507 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2354 2381 2474 10
Size: 54 1425 343 522 27 93 3186
Fragments arranged by size:
3186 1425 522 343 93 54 27
MslI CAynn'nnrTG
Cuts at: 518 1166 2306 2585 2846 3608 3967 4126 518
Size: 648 1140 279 261 762 359 159 2042
Fragments arranged by size:
2042 1140 762 648 359 279 261 159
MspA1I CmG'CkG
Cuts at: 1606 2437 2444 2699 3066 3743 4891 5136 1606
Size: 831 7 255 367 677 1148 245 2120
Fragments arranged by size:
2120 1148 831 677 367 255 245 7
NcoI C'CATG_G
Cuts at: 513 1065 2358 513
Size: 552 1293 3805
Fragments arranged by size:
3805 1293 552
NdeI CA'TA_TG
Cuts at: 387 2365 387
Size: 1978 3672
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5650
NotI GC'GGCC_GC
Cuts at: 3097 3097
Size: 5650
NspI r_CATG'y
Cuts at: 1132 1229 3008 4553 1132
Size: 97 1779 1545 2229
Fragments arranged by size:
2229 1779 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2932 1348
Size: 1584 4066
PciI A'CATG_T
Cuts at: 4549 4549
Size: 5650
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 2743 1108
Size: 902 733 4015
Fragments arranged by size:
4015 902 733
PfoI T'CCnGG_A
Cuts at: 2211 2211
Size: 5650
PmeI GTTT'AAAC
Cuts at: 2954 2954
Size: 5650
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3719 3751 5232 5264 3719
Size: 32 1481 32 4105
Fragments arranged by size:
4105 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2256 2928 2256
Size: 672 4978
PshAI GACnn'nnGTC
Cuts at: 1613 2027 1613
Size: 414 5236
PsiI TTA'TAA
Cuts at: 3220 3220
Size: 5650
PstI C_TGCA'G
Cuts at: 830 3002 830
Size: 2172 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5650
PvuII CAG'CTG
Cuts at: 2437 2444 2699 2437
Size: 7 255 5388
Fragments arranged by size:
5388 255 7
SalI G'TCGA_C
Cuts at: 1936 2992 1936
Size: 1056 4594
SanDI GG'GwC_CC
Cuts at: 2256 2256
Size: 5650
SbfI CC_TGCA'GG
Cuts at: 3002 3002
Size: 5650
ScaI AGT'ACT
Cuts at: 3898 5610 3898
Size: 1712 3938
SfcI C'TryA_G
Cuts at: 826 1045 2154 2761 2875 2998 4133 4814 5005
Size: 219 1109 607 114 123 1135 681 191
Cuts at: 5005 826
Size: 1471
Fragments arranged by size:
1471 1135 1109 681 607 219 191 123
114
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5650
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5650
SmaI CCC'GGG
Cuts at: 1872 2977 1872
Size: 1105 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2881 3754 4655 4917 820
Size: 197 926 938 873 901 262 1553
Fragments arranged by size:
1553 938 926 901 873 262 197
SnaBI TAC'GTA
Cuts at: 493 3441 493
Size: 2948 2702
SpeI A'CTAG_T
Cuts at: 152 2947 152
Size: 2795 2855
SphI G_CATG'C
Cuts at: 1132 3008 1132
Size: 1876 3774
SspI AAT'ATT
Cuts at: 5 52 3574 5
Size: 47 3522 2081
Fragments arranged by size:
3522 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5650
StyI C'CwwG_G
Cuts at: 513 1065 2290 2358 3089 513
Size: 552 1225 68 731 3074
Fragments arranged by size:
3074 1225 731 552 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3677 3694 3847 4032 4477 325
Size: 1255 2097 17 153 185 445 1498
Fragments arranged by size:
2097 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2184 3896 5608
Size: 274 80 33 51 1443 207 1712 1712
Cuts at: 5608 96
Size: 138
Fragments arranged by size:
1712 1712 1443 274 207 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5610 5642 5610
Size: 32 5618
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5650
XbaI T'CTAG_A
Cuts at: 2986 2986
Size: 5650
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5650
XmaI C'CCGG_G
Cuts at: 1870 2975 1870
Size: 1105 4545
XmnI GAAnn'nnTTC
Cuts at: 2040 2534 3779 2040
Size: 494 1245 3911
Fragments arranged by size:
3911 1245 494
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3458 276
Size: 53 83 186 936 1924 2468
Fragments arranged by size:
2468 1924 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SapI SexAI SfiI SfoI SrfI
SwaI XcmI