Restriction map for
pFN21AE6023
(Circular) (Six-base) MAPSORT of: pFN21AE6023.seq Check: 3801 from: 1 to: 5650
pFN21AE6023 5650 bp
With 182 enzymes: SgfI *
December 8, 2014 13:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3460 278
Size: 53 83 186 936 1924 2468
Fragments arranged by size:
2468 1924 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2993 1937
Size: 1056 4594
Acc65I G'GTAC_C
Cuts at: 2971 2971
Size: 5650
AclI AA'CG_TT
Cuts at: 1563 3777 4150 1563
Size: 2214 373 3063
Fragments arranged by size:
3063 2214 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2048 2715 3713 5097 5365 1632
Size: 416 667 998 1384 268 1917
Fragments arranged by size:
1917 1384 998 667 416 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5453
AflIII A'CryG_T
Cuts at: 1203 2577 4549 5526 1203
Size: 1374 1972 977 1327
Fragments arranged by size:
1972 1374 1327 977
AgeI A'CCGG_T
Cuts at: 4481 4481
Size: 5650
AhdI GACnn_n'nnGTC
Cuts at: 4379 4379
Size: 5650
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5650
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2464 1184
Size: 1280 4370
AlwNI CAG_nnn'CTG
Cuts at: 2033 3393 4965 2033
Size: 1360 1572 2718
Fragments arranged by size:
2718 1572 1360
ApaI G_GGCC'C
Cuts at: 2517 2517
Size: 5650
ApaLI G'TGCA_C
Cuts at: 3707 4863 3707
Size: 1156 4494
ApoI r'AATT_y
Cuts at: 1493 2036 2959 3185 3337 5446 1493
Size: 543 923 226 152 2109 1697
Fragments arranged by size:
2109 1697 923 543 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2135 4204 160
Size: 872 1103 2069 1606
Fragments arranged by size:
2069 1606 1103 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5650
AvaI C'yCGr_G
Cuts at: 1870 1943 2477 2975 1870
Size: 73 534 498 4545
Fragments arranged by size:
4545 534 498 73
BamHI G'GATC_C
Cuts at: 2980 3347 2980
Size: 367 5283
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2971 4426 618
Size: 325 217 627 1184 1455 1842
Fragments arranged by size:
1842 1455 1184 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2517 2969 1391
Size: 1126 452 4072
Fragments arranged by size:
4072 1126 452
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2734 2912 928
Size: 971 835 178 3666
Fragments arranged by size:
3666 971 835 178
BbvCI CC'TCA_GC
Cuts at: 2058 2058
Size: 5650
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3841 3875 1903
Size: 34 1904 34 3678
Fragments arranged by size:
3678 1904 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3542 4752 3542
Size: 1210 4440
BclI T'GATC_A
Cuts at: 1548 2406 1548
Size: 858 4792
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4261 136
Size: 107 122 71 631 605 2589 1525
Fragments arranged by size:
2589 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2214 2532 5645 2214
Size: 318 3113 2219
Fragments arranged by size:
3113 2219 318
BlpI GC'TnA_GC
Cuts at: 2558 3067 2558
Size: 509 5141
Bme1580I G_kGCm'C
Cuts at: 1790 2517 3711 4867 1790
Size: 727 1194 1156 2573
Fragments arranged by size:
2573 1194 1156 727
BmgBI CAC'GTC
Cuts at: 1318 2580 1318
Size: 1262 4388
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3381 4339 442
Size: 416 53 83 399 24 1964 958 1753
Fragments arranged by size:
1964 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5650
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2231 2964 4310 1239
Size: 992 733 1346 2579
Fragments arranged by size:
2579 1346 992 733
Bpu10I CC'TnA_GC
Cuts at: 2058 2058
Size: 5650
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3775 4640 4938 3775
Size: 865 298 4487
Fragments arranged by size:
4487 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4313 882
Size: 617 2814 2219
Fragments arranged by size:
2814 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3441 493
Size: 711 2237 2702
Fragments arranged by size:
2702 2237 711
BsaBI GATnn'nnATC
Cuts at: 5644 5644
Size: 5650
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3457 3839 4474 275
Size: 53 83 186 936 1924 382 635 1451
Fragments arranged by size:
1924 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4083 4481 4755 4902 5313 4083
Size: 398 274 147 411 4420
Fragments arranged by size:
4420 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2204 2234 1178
Size: 30 165 30 801 30 4594
Fragments arranged by size:
4594 801 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5650
BseYI C'CCAG_C
Cuts at: 4853 5349 4853
Size: 496 5154
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2745 1163
Size: 573 1009 4068
Fragments arranged by size:
4068 1009 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3100 3861 4889 1540
Size: 275 186 1099 761 1028 2301
Fragments arranged by size:
2301 1099 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2431 3711 3796 4867 2431
Size: 1280 85 1071 3214
Fragments arranged by size:
3214 1280 1071 85
BsmI GAATG_Cn'
Cuts at: 3161 3254 3161
Size: 93 5557
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2431 2517 2969 3711 3796 4867 1391
Size: 399 641 86 452 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 641 452 399 86 85
BspEI T'CCGG_A
Cuts at: 5313 5313
Size: 5650
BspHI T'CATG_A
Cuts at: 2409 3539 5269 2409
Size: 1130 1730 2790
Fragments arranged by size:
2790 1730 1130
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2432 3005 844
Size: 1588 573 3489
Fragments arranged by size:
3489 1588 573
BsrBI CCG'CTC
Cuts at: 1394 1823 2013 3537 1394
Size: 429 190 1524 3507
Fragments arranged by size:
3507 1524 429 190
BsrDI GCAATG_nn'
Cuts at: 65 1248 2640 2820 4145 4319 65
Size: 1183 1392 180 1325 174 1396
Fragments arranged by size:
1396 1392 1325 1183 180 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4294 4481 1700
Size: 110 2484 187 2869
Fragments arranged by size:
2869 2484 187 110
BsrGI T'GTAC_A
Cuts at: 96 2570 96
Size: 2474 3176
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5650
BssSI C'ACGA_G
Cuts at: 3710 4722 3710
Size: 1012 4638
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5650
BstBI TT'CG_AA
Cuts at: 4454 4454
Size: 5650
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5650
BstYI r'GATC_y
Cuts at: 1972 2214 2340 2532 2980 3347 3732 3749 5201
Size: 242 126 192 448 367 385 17 1452
Cuts at: 5201 5287 5299 5645 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 448 385 367 346 242 192
126 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2355 513
Size: 552 939 351 3808
Fragments arranged by size:
3808 939 552 351
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4911
Fragments arranged by size:
4911 683 56
BtsI GCAGTG_nn'
Cuts at: 2498 2767 3166 3960 3980 2498
Size: 269 399 794 20 4168
Fragments arranged by size:
4168 794 399 269 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5650
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2790 2825 529
Size: 35 2226 35 3354
Fragments arranged by size:
3354 2226 35 35
DraI TTT'AAA
Cuts at: 2954 2954
Size: 5650
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4657 809
Size: 507 3341 1802
Fragments arranged by size:
3341 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2358 3097 3986
Size: 54 1425 50 275 18 528 739 889
Cuts at: 3986 5615 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 739 528 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3097 1537
Size: 275 1285 4090
Fragments arranged by size:
4090 1285 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2397 2459 2695 3580 1353
Size: 1044 62 236 885 3423
Fragments arranged by size:
3423 1044 885 236 62
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4237 4611 4757 387
Size: 1064 138 2648 374 146 1280
Fragments arranged by size:
2648 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2048 2231 2715 2964 3713 4310 5097
Size: 393 416 183 484 249 749 597 787
Cuts at: 5097 5365 1239
Size: 268 1524
Fragments arranged by size:
1524 787 749 597 484 416 393 268
249 183
EcoRI G'AATT_C
Cuts at: 2959 3337 2959
Size: 378 5272
FspI TGC'GCA
Cuts at: 4156 4156
Size: 5650
HaeII r_GCGC'y
Cuts at: 1678 4797 1678
Size: 3119 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4291 4323 4365 4397 1373
Size: 32 135 32 2719 32 42 32 2626
Fragments arranged by size:
2719 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2994 3240 669
Size: 988 281 1056 246 3079
Fragments arranged by size:
3079 1056 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5650
HpaI GTT'AAC
Cuts at: 3240 3240
Size: 5650
KpnI G_GTAC'C
Cuts at: 2975 2975
Size: 5650
MfeI C'AATT_G
Cuts at: 3249 3249
Size: 5650
MluI A'CGCG_T
Cuts at: 5526 5526
Size: 5650
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2277 4764 4948 1305
Size: 972 2487 184 2007
Fragments arranged by size:
2487 2007 972 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2360 10
Size: 54 1425 343 528 3300
Fragments arranged by size:
3300 1425 528 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2282 2582 3608 3967 4126 518
Size: 648 1116 300 1026 359 159 2042
Fragments arranged by size:
2042 1116 1026 648 359 300 159
MspA1I CmG'CkG
Cuts at: 1606 3066 3743 4891 5136 1606
Size: 1460 677 1148 245 2120
Fragments arranged by size:
2120 1460 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2355 513
Size: 552 939 351 3808
Fragments arranged by size:
3808 939 552 351
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5650
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5650
NotI GC'GGCC_GC
Cuts at: 3097 3097
Size: 5650
NspI r_CATG'y
Cuts at: 1132 1229 3008 4553 1132
Size: 97 1779 1545 2229
Fragments arranged by size:
2229 1779 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5650
PciI A'CATG_T
Cuts at: 4549 4549
Size: 5650
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2737 1108
Size: 1629 4021
PmeI GTTT'AAAC
Cuts at: 2954 2954
Size: 5650
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3719 3751 5232 5264 3719
Size: 32 1481 32 4105
Fragments arranged by size:
4105 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5650
PsiI TTA'TAA
Cuts at: 3220 3220
Size: 5650
PspOMI G'GGCC_C
Cuts at: 2513 2513
Size: 5650
PstI C_TGCA'G
Cuts at: 830 2443 3002 830
Size: 1613 559 3478
Fragments arranged by size:
3478 1613 559
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5650
SalI G'TCGA_C
Cuts at: 1936 2992 1936
Size: 1056 4594
SbfI CC_TGCA'GG
Cuts at: 3002 3002
Size: 5650
ScaI AGT'ACT
Cuts at: 3898 5610 3898
Size: 1712 3938
SfcI C'TryA_G
Cuts at: 826 1045 2439 2998 4133 4814 5005 826
Size: 219 1394 559 1135 681 191 1471
Fragments arranged by size:
1471 1394 1135 681 559 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5650
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5650
SmaI CCC'GGG
Cuts at: 1872 2977 1872
Size: 1105 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3754 4655 4917 820
Size: 197 926 1811 901 262 1553
Fragments arranged by size:
1811 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3441 493
Size: 2948 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5650
SphI G_CATG'C
Cuts at: 1132 3008 1132
Size: 1876 3774
SspI AAT'ATT
Cuts at: 5 52 3574 5
Size: 47 3522 2081
Fragments arranged by size:
3522 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5650
StyI C'CwwG_G
Cuts at: 513 1065 2004 2355 3089 513
Size: 552 939 351 734 3074
Fragments arranged by size:
3074 939 734 552 351
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2177 3677 3694 3847 4032 4477 325
Size: 1255 597 1500 17 153 185 445 1498
Fragments arranged by size:
1500 1498 1255 597 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2082 2570 2775
Size: 274 80 33 51 1443 105 488 205
Cuts at: 2775 2839 3896 5608 96
Size: 64 1057 1712 138
Fragments arranged by size:
1712 1443 1057 488 274 205 138 105
80 64 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5610 5642 5610
Size: 32 5618
Tth111I GACn'n_nGTC
Cuts at: 1316 2546 1316
Size: 1230 4420
XbaI T'CTAG_A
Cuts at: 2986 2986
Size: 5650
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5650
XmaI C'CCGG_G
Cuts at: 1870 2975 1870
Size: 1105 4545
XmnI GAAnn'nnTTC
Cuts at: 3779 3779
Size: 5650
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3458 276
Size: 53 83 186 936 1924 2468
Fragments arranged by size:
2468 1924 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI