Restriction map for
pFN21AE6022
(Circular) (Six-base) MAPSORT of: pFN21AE6022.seq Check: 7535 from: 1 to: 5650
pFN21AE6022 5650 bp
With 182 enzymes: SgfI *
December 8, 2014 13:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2744 2744
Size: 5650
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3460 278
Size: 53 83 186 936 1924 2468
Fragments arranged by size:
2468 1924 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2784 2993 1937
Size: 847 209 4594
Fragments arranged by size:
4594 847 209
Acc65I G'GTAC_C
Cuts at: 2971 2971
Size: 5650
AclI AA'CG_TT
Cuts at: 1563 3777 4150 1563
Size: 2214 373 3063
Fragments arranged by size:
3063 2214 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2053 2144 2939 3713 5097 5365 1632
Size: 421 91 795 774 1384 268 1917
Fragments arranged by size:
1917 1384 795 774 421 268 91
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5453
AflIII A'CryG_T
Cuts at: 1203 4549 5526 1203
Size: 3346 977 1327
Fragments arranged by size:
3346 1327 977
AgeI A'CCGG_T
Cuts at: 4481 4481
Size: 5650
AhdI GACnn_n'nnGTC
Cuts at: 4379 4379
Size: 5650
AleI CACnn'nnGTG
Cuts at: 1166 2864 1166
Size: 1698 3952
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2473 1184
Size: 1289 4361
AlwNI CAG_nnn'CTG
Cuts at: 2300 2529 3393 4965 2300
Size: 229 864 1572 2985
Fragments arranged by size:
2985 1572 864 229
ApaLI G'TGCA_C
Cuts at: 3707 4863 3707
Size: 1156 4494
ApoI r'AATT_y
Cuts at: 1493 2348 2959 3185 3337 5446 1493
Size: 855 611 226 152 2109 1697
Fragments arranged by size:
2109 1697 855 611 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4204 160
Size: 872 3172 1606
Fragments arranged by size:
3172 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5650
AvaI C'yCGr_G
Cuts at: 1870 1943 2975 1870
Size: 73 1032 4545
Fragments arranged by size:
4545 1032 73
BamHI G'GATC_C
Cuts at: 2980 3347 2980
Size: 367 5283
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2971 4426 618
Size: 325 217 627 1184 1455 1842
Fragments arranged by size:
1842 1455 1184 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2474 2917 2969 1391
Size: 1083 443 52 4072
Fragments arranged by size:
4072 1083 443 52
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2836 928
Size: 971 937 3742
Fragments arranged by size:
3742 971 937
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3841 3875 1903
Size: 34 1904 34 3678
Fragments arranged by size:
3678 1904 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3542 4752 3542
Size: 1210 4440
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5650
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2369 4261 136
Size: 107 122 71 631 605 697 1892 1525
Fragments arranged by size:
1892 1525 697 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5645 5645
Size: 5650
BlpI GC'TnA_GC
Cuts at: 3067 3067
Size: 5650
Bme1580I G_kGCm'C
Cuts at: 1790 2253 3711 4867 1790
Size: 463 1458 1156 2573
Fragments arranged by size:
2573 1458 1156 463
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5650
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3381 4339 442
Size: 416 53 83 399 24 1964 958 1753
Fragments arranged by size:
1964 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5650
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4310 1239
Size: 3071 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3775 4640 4938 3775
Size: 865 298 4487
Fragments arranged by size:
4487 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2296 2597 4313 882
Size: 617 797 301 1716 2219
Fragments arranged by size:
2219 1716 797 617 301
BsaAI yAC'GTr
Cuts at: 493 1204 2386 3441 493
Size: 711 1182 1055 2702
Fragments arranged by size:
2702 1182 1055 711
BsaBI GATnn'nnATC
Cuts at: 5644 5644
Size: 5650
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2172 3457 3839 4474
Size: 53 83 186 936 639 1285 382 635
Cuts at: 4474 275
Size: 1451
Fragments arranged by size:
1451 1285 936 639 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2863 4083 4481 4755 4902 5313 2863
Size: 1220 398 274 147 411 3200
Fragments arranged by size:
3200 1220 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2664 2694 1178
Size: 30 165 30 1261 30 4134
Fragments arranged by size:
4134 1261 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2627 2672 2731 1186
Size: 1441 45 59 4105
Fragments arranged by size:
4105 1441 59 45
BseYI C'CCAG_C
Cuts at: 2801 4853 5349 2801
Size: 2052 496 3102
Fragments arranged by size:
3102 2052 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5077
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3100 3861 4889 1540
Size: 275 186 1099 761 1028 2301
Fragments arranged by size:
2301 1099 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3711 3796 4867 3711
Size: 85 1071 4494
Fragments arranged by size:
4494 1071 85
BsmI GAATG_Cn'
Cuts at: 3161 3254 3161
Size: 93 5557
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2253 2474 2917 2969 3711 3796 4867
Size: 399 463 221 443 52 742 85 1071
Cuts at: 4867 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 463 443 399 221 85
52
BspEI T'CCGG_A
Cuts at: 5313 5313
Size: 5650
BspHI T'CATG_A
Cuts at: 2421 3539 5269 2421
Size: 1118 1730 2802
Fragments arranged by size:
2802 1730 1118
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2330 2744 3005 844
Size: 1486 414 261 3489
Fragments arranged by size:
3489 1486 414 261
BsrBI CCG'CTC
Cuts at: 1394 1823 3537 1394
Size: 429 1714 3507
Fragments arranged by size:
3507 1714 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4145 4319 65
Size: 1183 2897 174 1396
Fragments arranged by size:
2897 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4294 4481 1700
Size: 110 2484 187 2869
Fragments arranged by size:
2869 2484 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5650
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5650
BssSI C'ACGA_G
Cuts at: 3710 4722 3710
Size: 1012 4638
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5650
BstBI TT'CG_AA
Cuts at: 4454 4454
Size: 5650
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5650
BstYI r'GATC_y
Cuts at: 1972 2525 2980 3347 3732 3749 5201 5287 5299
Size: 553 455 367 385 17 1452 86 12
Cuts at: 5299 5645 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 553 455 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2367 513
Size: 552 939 363 3796
Fragments arranged by size:
3796 939 552 363
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4911
Fragments arranged by size:
4911 683 56
BtsI GCAGTG_nn'
Cuts at: 3166 3960 3980 3166
Size: 794 20 4836
Fragments arranged by size:
4836 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5650
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5615
DraI TTT'AAA
Cuts at: 2954 2954
Size: 5650
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4657 809
Size: 507 3341 1802
Fragments arranged by size:
3341 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2025 2388
Size: 54 1425 50 275 18 177 18 363
Cuts at: 2388 3097 3986 5615 8
Size: 709 889 1629 43
Fragments arranged by size:
1629 1425 889 709 363 275 177 54
50 43 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 3097 1537
Size: 275 1285 4090
Fragments arranged by size:
4090 1285 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2072 2771 3580 1353
Size: 719 699 809 3423
Fragments arranged by size:
3423 809 719 699
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4237 4611 4757 387
Size: 1064 138 2648 374 146 1280
Fragments arranged by size:
2648 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2053 2144 2939 3713 4310 5097 5365
Size: 393 421 91 795 774 597 787 268
Cuts at: 5365 1239
Size: 1524
Fragments arranged by size:
1524 795 787 774 597 421 393 268
91
EcoNI CCTnn'n_nnAGG
Cuts at: 2267 2267
Size: 5650
EcoRI G'AATT_C
Cuts at: 2959 3337 2959
Size: 378 5272
FspI TGC'GCA
Cuts at: 4156 4156
Size: 5650
HaeII r_GCGC'y
Cuts at: 1678 4797 1678
Size: 3119 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4291 4323 4365 4397 1373
Size: 32 135 32 2719 32 42 32 2626
Fragments arranged by size:
2719 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2931 2994 3240 669
Size: 988 281 993 63 246 3079
Fragments arranged by size:
3079 993 988 281 246 63
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5650
HpaI GTT'AAC
Cuts at: 2931 3240 2931
Size: 309 5341
KpnI G_GTAC'C
Cuts at: 2975 2975
Size: 5650
MfeI C'AATT_G
Cuts at: 2210 3249 2210
Size: 1039 4611
MluI A'CGCG_T
Cuts at: 5526 5526
Size: 5650
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2025 4764 4948 1305
Size: 720 2739 184 2007
Fragments arranged by size:
2739 2007 720 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2027 2390 10
Size: 54 1425 343 177 18 363 3270
Fragments arranged by size:
3270 1425 363 343 177 54 18
MslI CAynn'nnrTG
Cuts at: 518 1166 2021 2057 2111 2225 2279 2504 2645
Size: 648 855 36 54 114 54 225 141
Cuts at: 2645 2792 2864 3608 3967 4126 518
Size: 147 72 744 359 159 2042
Fragments arranged by size:
2042 855 744 648 359 225 159 147
141 114 72 54 54 36
MspA1I CmG'CkG
Cuts at: 1606 3066 3743 4891 5136 1606
Size: 1460 677 1148 245 2120
Fragments arranged by size:
2120 1460 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2367 513
Size: 552 939 363 3796
Fragments arranged by size:
3796 939 552 363
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5650
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5650
NotI GC'GGCC_GC
Cuts at: 3097 3097
Size: 5650
NspI r_CATG'y
Cuts at: 1132 1229 2797 3008 4553 1132
Size: 97 1568 211 1545 2229
Fragments arranged by size:
2229 1568 1545 211 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5650
PciI A'CATG_T
Cuts at: 4549 4549
Size: 5650
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2275 1108
Size: 1167 4483
PfoI T'CCnGG_A
Cuts at: 2262 2262
Size: 5650
PmeI GTTT'AAAC
Cuts at: 2954 2954
Size: 5650
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3719 3751 5232 5264 3719
Size: 32 1481 32 4105
Fragments arranged by size:
4105 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2756 1613
Size: 1143 4507
PsiI TTA'TAA
Cuts at: 3220 3220
Size: 5650
PstI C_TGCA'G
Cuts at: 830 2525 3002 830
Size: 1695 477 3478
Fragments arranged by size:
3478 1695 477
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5650
SalI G'TCGA_C
Cuts at: 1936 2992 1936
Size: 1056 4594
SbfI CC_TGCA'GG
Cuts at: 3002 3002
Size: 5650
ScaI AGT'ACT
Cuts at: 3898 5610 3898
Size: 1712 3938
SexAI A'CCwGG_T
Cuts at: 2268 2268
Size: 5650
SfcI C'TryA_G
Cuts at: 826 1045 2521 2884 2998 4133 4814 5005 826
Size: 219 1476 363 114 1135 681 191 1471
Fragments arranged by size:
1476 1471 1135 681 363 219 191 114
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5650
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5650
SmaI CCC'GGG
Cuts at: 1872 2977 1872
Size: 1105 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3754 4655 4917 820
Size: 197 926 1811 901 262 1553
Fragments arranged by size:
1811 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3441 493
Size: 2948 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5650
SphI G_CATG'C
Cuts at: 1132 3008 1132
Size: 1876 3774
SspI AAT'ATT
Cuts at: 5 52 3574 5
Size: 47 3522 2081
Fragments arranged by size:
3522 2081 47
StuI AGG'CCT
Cuts at: 1517 2726 1517
Size: 1209 4441
StyI C'CwwG_G
Cuts at: 513 1065 2004 2367 3089 513
Size: 552 939 363 722 3074
Fragments arranged by size:
3074 939 722 552 363
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2267 3677 3694 3847 4032 4477 325
Size: 1255 687 1410 17 153 185 445 1498
Fragments arranged by size:
1498 1410 1255 687 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2193 3896 5608
Size: 274 80 33 51 1443 216 1703 1712
Cuts at: 5608 96
Size: 138
Fragments arranged by size:
1712 1703 1443 274 216 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5610 5642 5610
Size: 32 5618
Tth111I GACn'n_nGTC
Cuts at: 1316 2270 1316
Size: 954 4696
XbaI T'CTAG_A
Cuts at: 2986 2986
Size: 5650
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2260 2260
Size: 5650
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5650
XmaI C'CCGG_G
Cuts at: 1870 2975 1870
Size: 1105 4545
XmnI GAAnn'nnTTC
Cuts at: 3779 3779
Size: 5650
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3458 276
Size: 53 83 186 936 1924 2468
Fragments arranged by size:
2468 1924 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SfiI SfoI SrfI SwaI