Restriction map for
pFN21AE6013
(Circular) (Six-base) MAPSORT of: pFN21AE6013.seq Check: 6482 from: 1 to: 5647
pFN21AE6013 5647 bp
With 182 enzymes: SgfI *
December 8, 2014 13:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3457 278
Size: 53 83 186 936 1921 2468
Fragments arranged by size:
2468 1921 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2624 2990 1937
Size: 687 366 4594
Fragments arranged by size:
4594 687 366
Acc65I G'GTAC_C
Cuts at: 2968 2968
Size: 5647
AclI AA'CG_TT
Cuts at: 1563 3774 4147 1563
Size: 2211 373 3063
Fragments arranged by size:
3063 2211 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3710 5094 5362 1632
Size: 2078 1384 268 1917
Fragments arranged by size:
2078 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5450
AflIII A'CryG_T
Cuts at: 1203 2082 4546 5523 1203
Size: 879 2464 977 1327
Fragments arranged by size:
2464 1327 977 879
AgeI A'CCGG_T
Cuts at: 4478 4478
Size: 5647
AhdI GACnn_n'nnGTC
Cuts at: 4376 4376
Size: 5647
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5647
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5647
AlwNI CAG_nnn'CTG
Cuts at: 3390 4962 3390
Size: 1572 4075
ApaLI G'TGCA_C
Cuts at: 2236 3704 4860 2236
Size: 1468 1156 3023
Fragments arranged by size:
3023 1468 1156
ApoI r'AATT_y
Cuts at: 1493 2439 2956 3182 3334 5443 1493
Size: 946 517 226 152 2109 1697
Fragments arranged by size:
2109 1697 946 517 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4201 160
Size: 872 3169 1606
Fragments arranged by size:
3169 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5647
AvaI C'yCGr_G
Cuts at: 1870 1943 2972 1870
Size: 73 1029 4545
Fragments arranged by size:
4545 1029 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2171 2204 2171
Size: 33 5614
BamHI G'GATC_C
Cuts at: 2977 3344 2977
Size: 367 5280
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2968 4423 618
Size: 325 217 627 1181 1455 1842
Fragments arranged by size:
1842 1455 1181 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2966 1391
Size: 1575 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2035 2677 928
Size: 971 136 642 3898
Fragments arranged by size:
3898 971 642 136
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3838 3872 1903
Size: 34 50 34 1817 34 3678
Fragments arranged by size:
3678 1817 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2834 3539 4749 2834
Size: 705 1210 3732
Fragments arranged by size:
3732 1210 705
BclI T'GATC_A
Cuts at: 1548 2430 1548
Size: 882 4765
BfrBI ATG'CAT
Cuts at: 2573 2573
Size: 5647
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4258 136
Size: 107 122 71 631 605 2586 1525
Fragments arranged by size:
2586 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5642 5642
Size: 5647
BlpI GC'TnA_GC
Cuts at: 3064 3064
Size: 5647
Bme1580I G_kGCm'C
Cuts at: 1790 2240 3708 4864 1790
Size: 450 1468 1156 2573
Fragments arranged by size:
2573 1468 1156 450
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5647
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3378 4336 442
Size: 416 53 83 399 24 1961 958 1753
Fragments arranged by size:
1961 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5647
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2232 2685 4307 1239
Size: 993 453 1622 2579
Fragments arranged by size:
2579 1622 993 453
Bpu10I CC'TnA_GC
Cuts at: 2254 2254
Size: 5647
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3772 4637 4935 3772
Size: 865 298 4484
Fragments arranged by size:
4484 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4310 882
Size: 617 2811 2219
Fragments arranged by size:
2811 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2235 3438 493
Size: 711 1031 1203 2702
Fragments arranged by size:
2702 1203 1031 711
BsaBI GATnn'nnATC
Cuts at: 2093 5641 2093
Size: 3548 2099
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2806 3454 3836 4471
Size: 53 83 186 936 1273 648 382 635
Cuts at: 4471 275
Size: 1451
Fragments arranged by size:
1451 1273 936 648 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4080 4478 4752 4899 5310 4080
Size: 398 274 147 411 4417
Fragments arranged by size:
4417 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5422
Fragments arranged by size:
5422 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5647
BseYI C'CCAG_C
Cuts at: 2053 2276 4850 5346 2053
Size: 223 2574 496 2354
Fragments arranged by size:
2574 2354 496 223
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5074
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3097 3858 4886 1540
Size: 275 186 1096 761 1028 2301
Fragments arranged by size:
2301 1096 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2240 3708 3793 4864 2240
Size: 1468 85 1071 3023
Fragments arranged by size:
3023 1468 1071 85
BsmI GAATG_Cn'
Cuts at: 2850 3158 3251 2850
Size: 308 93 5246
Fragments arranged by size:
5246 308 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2240 2966 3708 3793 4864 1391
Size: 399 450 726 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 726 450 399 85
BspEI T'CCGG_A
Cuts at: 5310 5310
Size: 5647
BspHI T'CATG_A
Cuts at: 3536 5266 3536
Size: 1730 3917
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3002 844
Size: 2158 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3534 1394
Size: 429 1711 3507
Fragments arranged by size:
3507 1711 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4142 4316 65
Size: 1183 2894 174 1396
Fragments arranged by size:
2894 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4291 4478 1700
Size: 110 2481 187 2869
Fragments arranged by size:
2869 2481 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5647
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5647
BssSI C'ACGA_G
Cuts at: 3707 4719 3707
Size: 1012 4635
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5647
BstBI TT'CG_AA
Cuts at: 2587 4451 2587
Size: 1864 3783
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5647
BstXI CCAn_nnnn'nTGG
Cuts at: 2801 2801
Size: 5647
BstYI r'GATC_y
Cuts at: 1972 2349 2977 3344 3729 3746 5198 5284 5296
Size: 377 628 367 385 17 1452 86 12
Cuts at: 5296 5642 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 628 385 377 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2247 2247
Size: 5647
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5095
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4908
Fragments arranged by size:
4908 683 56
BtsI GCAGTG_nn'
Cuts at: 3163 3957 3977 3163
Size: 794 20 4833
Fragments arranged by size:
4833 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5647
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5612
DraI TTT'AAA
Cuts at: 2499 2951 2499
Size: 452 5195
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4654 809
Size: 507 3338 1802
Fragments arranged by size:
3338 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3094 3983 5612
Size: 54 1425 50 275 18 1264 889 1629
Cuts at: 5612 8
Size: 43
Fragments arranged by size:
1629 1425 1264 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3094 1537
Size: 275 1282 4090
Fragments arranged by size:
4090 1282 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2198 2318 3577 1353
Size: 845 120 1259 3423
Fragments arranged by size:
3423 1259 845 120
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4234 4608 4754 387
Size: 1064 138 2645 374 146 1280
Fragments arranged by size:
2645 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2232 2685 3710 4307 5094 5362 1239
Size: 393 600 453 1025 597 787 268 1524
Fragments arranged by size:
1524 1025 787 600 597 453 393 268
EcoRI G'AATT_C
Cuts at: 2956 3334 2956
Size: 378 5269
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2526 2526
Size: 5647
FspI TGC'GCA
Cuts at: 4153 4153
Size: 5647
HaeII r_GCGC'y
Cuts at: 1678 4794 1678
Size: 3116 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2440 2472 4288 4320 4362
Size: 32 135 32 868 32 1816 32 42
Cuts at: 4362 4394 1373
Size: 32 2626
Fragments arranged by size:
2626 1816 868 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2625 2991 3237 669
Size: 988 281 687 366 246 3079
Fragments arranged by size:
3079 988 687 366 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5647
HpaI GTT'AAC
Cuts at: 3237 3237
Size: 5647
KpnI G_GTAC'C
Cuts at: 2972 2972
Size: 5647
MfeI C'AATT_G
Cuts at: 3246 3246
Size: 5647
MluI A'CGCG_T
Cuts at: 5523 5523
Size: 5647
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4761 4945 1305
Size: 3456 184 2007
Fragments arranged by size:
3456 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3825
Fragments arranged by size:
3825 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2618 3605 3964 4123 518
Size: 648 1452 987 359 159 2042
Fragments arranged by size:
2042 1452 987 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3063 3740 4888 5133 1606
Size: 1457 677 1148 245 2120
Fragments arranged by size:
2120 1457 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5095
NdeI CA'TA_TG
Cuts at: 387 2387 387
Size: 2000 3647
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5647
NotI GC'GGCC_GC
Cuts at: 3094 3094
Size: 5647
NsiI A_TGCA'T
Cuts at: 2575 2575
Size: 5647
NspI r_CATG'y
Cuts at: 1132 1229 2086 2206 2577 2695 3005 4550 1132
Size: 97 857 120 371 118 310 1545 2229
Fragments arranged by size:
2229 1545 857 371 310 120 118 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5647
PciI A'CATG_T
Cuts at: 2082 4546 2082
Size: 2464 3183
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2053 1108
Size: 945 4702
PfoI T'CCnGG_A
Cuts at: 2907 2907
Size: 5647
PmeI GTTT'AAAC
Cuts at: 2951 2951
Size: 5647
PmlI CAC'GTG
Cuts at: 2235 2235
Size: 5647
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3716 3748 5229 5261 3716
Size: 32 1481 32 4102
Fragments arranged by size:
4102 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2630 1613
Size: 1017 4630
PsiI TTA'TAA
Cuts at: 2936 3217 2936
Size: 281 5366
PstI C_TGCA'G
Cuts at: 830 2999 830
Size: 2169 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5647
SalI G'TCGA_C
Cuts at: 1936 2623 2989 1936
Size: 687 366 4594
Fragments arranged by size:
4594 687 366
SapI GCTCTTCn'nnn_
Cuts at: 2318 2318
Size: 5647
SbfI CC_TGCA'GG
Cuts at: 2999 2999
Size: 5647
ScaI AGT'ACT
Cuts at: 3895 5607 3895
Size: 1712 3935
SfcI C'TryA_G
Cuts at: 826 1045 2069 2995 4130 4811 5002 826
Size: 219 1024 926 1135 681 191 1471
Fragments arranged by size:
1471 1135 1024 926 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5647
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5647
SmaI CCC'GGG
Cuts at: 1872 2974 1872
Size: 1102 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3751 4652 4914 820
Size: 197 926 1808 901 262 1553
Fragments arranged by size:
1808 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3438 493
Size: 2945 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5647
SphI G_CATG'C
Cuts at: 1132 2206 3005 1132
Size: 1074 799 3774
Fragments arranged by size:
3774 1074 799
SspI AAT'ATT
Cuts at: 5 52 2527 3571 5
Size: 47 2475 1044 2081
Fragments arranged by size:
2475 2081 1044 47
StuI AGG'CCT
Cuts at: 1517 2252 1517
Size: 735 4912
StyI C'CwwG_G
Cuts at: 513 1065 3086 513
Size: 552 2021 3074
Fragments arranged by size:
3074 2021 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3674 3691 3844 4029 4474 325
Size: 1255 2094 17 153 185 445 1498
Fragments arranged by size:
2094 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2644 3893 5605
Size: 274 80 33 51 1443 667 1249 1712
Cuts at: 5605 96
Size: 138
Fragments arranged by size:
1712 1443 1249 667 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2808 2840 5607 5639 2808
Size: 32 2767 32 2816
Fragments arranged by size:
2816 2767 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2222 1316
Size: 906 4741
XbaI T'CTAG_A
Cuts at: 2983 2983
Size: 5647
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5647
XmaI C'CCGG_G
Cuts at: 1870 2972 1870
Size: 1102 4545
XmnI GAAnn'nnTTC
Cuts at: 3776 3776
Size: 5647
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3455 276
Size: 53 83 186 936 1921 2468
Fragments arranged by size:
2468 1921 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PstI PvuI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI