Restriction map for
pFN21AE6004
(Circular) (Six-base) MAPSORT of: pFN21AE6004.seq Check: 1714 from: 1 to: 5647
pFN21AE6004 5647 bp
With 182 enzymes: SgfI *
December 8, 2014 13:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2732 2732
Size: 5647
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3457 278
Size: 53 83 186 936 1921 2468
Fragments arranged by size:
2468 1921 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2990 1937
Size: 1053 4594
Acc65I G'GTAC_C
Cuts at: 2968 2968
Size: 5647
AclI AA'CG_TT
Cuts at: 1563 3774 4147 1563
Size: 2211 373 3063
Fragments arranged by size:
3063 2211 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2041 2579 3710 5094 5362 1632
Size: 409 538 1131 1384 268 1917
Fragments arranged by size:
1917 1384 1131 538 409 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5450
AflIII A'CryG_T
Cuts at: 1203 2664 4546 5523 1203
Size: 1461 1882 977 1327
Fragments arranged by size:
1882 1461 1327 977
AgeI A'CCGG_T
Cuts at: 4478 4478
Size: 5647
AhdI GACnn_n'nnGTC
Cuts at: 4376 4376
Size: 5647
AleI CACnn'nnGTG
Cuts at: 1166 2744 1166
Size: 1578 4069
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5647
AlwNI CAG_nnn'CTG
Cuts at: 2392 3390 4962 2392
Size: 998 1572 3077
Fragments arranged by size:
3077 1572 998
ApaLI G'TGCA_C
Cuts at: 3704 4860 3704
Size: 1156 4491
ApoI r'AATT_y
Cuts at: 1493 2956 3182 3334 5443 1493
Size: 1463 226 152 2109 1697
Fragments arranged by size:
2109 1697 1463 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4201 160
Size: 872 3169 1606
Fragments arranged by size:
3169 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5647
AvaI C'yCGr_G
Cuts at: 1870 1943 2972 1870
Size: 73 1029 4545
Fragments arranged by size:
4545 1029 73
BamHI G'GATC_C
Cuts at: 2977 3344 2977
Size: 367 5280
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2273 2545 2762 2785 2968
Size: 325 217 627 486 272 217 23 183
Cuts at: 2968 4423 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 486 325 272 217 217
183 23
BanII G_rGCy'C
Cuts at: 1391 2444 2754 2966 1391
Size: 1053 310 212 4072
Fragments arranged by size:
4072 1053 310 212
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4676
BbvCI CC'TCA_GC
Cuts at: 2334 2334
Size: 5647
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3838 3872 1903
Size: 34 1901 34 3678
Fragments arranged by size:
3678 1901 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2409 3539 4749 2409
Size: 1130 1210 3307
Fragments arranged by size:
3307 1210 1130
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5647
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2124 4258 136
Size: 107 122 71 631 605 452 2134 1525
Fragments arranged by size:
2134 1525 631 605 452 122 107 71
BglII A'GATC_T
Cuts at: 5642 5642
Size: 5647
BlpI GC'TnA_GC
Cuts at: 2192 2918 3064 2192
Size: 726 146 4775
Fragments arranged by size:
4775 726 146
Bme1580I G_kGCm'C
Cuts at: 1790 3708 4864 1790
Size: 1918 1156 2573
Fragments arranged by size:
2573 1918 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5647
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3378 4336 442
Size: 416 53 83 399 24 1961 958 1753
Fragments arranged by size:
1961 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5647
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2948 2948
Size: 5647
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4307 1239
Size: 3068 2579
Bpu10I CC'TnA_GC
Cuts at: 2334 2334
Size: 5647
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3772 4637 4935 3772
Size: 865 298 4484
Fragments arranged by size:
4484 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4310 882
Size: 617 2811 2219
Fragments arranged by size:
2811 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2665 3438 493
Size: 711 1461 773 2702
Fragments arranged by size:
2702 1461 773 711
BsaBI GATnn'nnATC
Cuts at: 2016 5641 2016
Size: 3625 2022
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2340 2416 3454 3836
Size: 53 83 186 936 807 76 1038 382
Cuts at: 3836 4471 275
Size: 635 1451
Fragments arranged by size:
1451 1038 936 807 635 382 186 83
76 53
BsaWI w'CCGG_w
Cuts at: 2887 4080 4478 4752 4899 5310 2887
Size: 1193 398 274 147 411 3224
Fragments arranged by size:
3224 1193 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2064 2094 2498 2528 1178
Size: 30 165 30 661 30 404 30 4297
Fragments arranged by size:
4297 661 404 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2072 2434 2711 2719 1186
Size: 886 362 277 8 4114
Fragments arranged by size:
4114 886 362 277 8
BseYI C'CCAG_C
Cuts at: 4850 5346 4850
Size: 496 5151
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2316 2592 1163
Size: 573 580 276 4218
Fragments arranged by size:
4218 580 573 276
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3097 3858 4886 1540
Size: 275 186 1096 761 1028 2301
Fragments arranged by size:
2301 1096 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2466 3708 3793 4864 2466
Size: 1242 85 1071 3249
Fragments arranged by size:
3249 1242 1071 85
BsmI GAATG_Cn'
Cuts at: 3158 3251 3158
Size: 93 5554
BsmBI CGTCTCn'nnnn_
Cuts at: 2407 2407
Size: 5647
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2444 2466 2754 2966 3708 3793 4864
Size: 399 654 22 288 212 742 85 1071
Cuts at: 4864 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 654 399 288 212 85
22
BspEI T'CCGG_A
Cuts at: 2887 5310 2887
Size: 2423 3224
BspHI T'CATG_A
Cuts at: 3536 5266 3536
Size: 1730 3917
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2732 3002 844
Size: 1888 270 3489
Fragments arranged by size:
3489 1888 270
BsrBI CCG'CTC
Cuts at: 1394 1823 3534 1394
Size: 429 1711 3507
Fragments arranged by size:
3507 1711 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4142 4316 65
Size: 1183 2894 174 1396
Fragments arranged by size:
2894 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4291 4478 1700
Size: 110 2481 187 2869
Fragments arranged by size:
2869 2481 187 110
BsrGI T'GTAC_A
Cuts at: 96 2778 96
Size: 2682 2965
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5647
BssSI C'ACGA_G
Cuts at: 3707 4719 3707
Size: 1012 4635
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5647
BstBI TT'CG_AA
Cuts at: 4451 4451
Size: 5647
BstEII G'GTnAC_C
Cuts at: 1184 2230 1184
Size: 1046 4601
BstYI r'GATC_y
Cuts at: 1972 2977 3344 3729 3746 5198 5284 5296 5642
Size: 1005 367 385 17 1452 86 12 346
Cuts at: 5642 1972
Size: 1977
Fragments arranged by size:
1977 1452 1005 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2608 2608
Size: 5647
BtgI C'CryG_G
Cuts at: 513 1065 2004 2226 513
Size: 552 939 222 3934
Fragments arranged by size:
3934 939 552 222
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2629 487
Size: 683 56 1403 3505
Fragments arranged by size:
3505 1403 683 56
BtsI GCAGTG_nn'
Cuts at: 3163 3957 3977 3163
Size: 794 20 4833
Fragments arranged by size:
4833 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5647
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5612
DraI TTT'AAA
Cuts at: 2951 2951
Size: 5647
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4654 809
Size: 507 3338 1802
Fragments arranged by size:
3338 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2659 3094 3983
Size: 54 1425 50 275 18 829 435 889
Cuts at: 3983 5612 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 829 435 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3094 1537
Size: 275 1282 4090
Fragments arranged by size:
4090 1282 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2060 2090 2147 2837 3577 1353
Size: 707 30 57 690 740 3423
Fragments arranged by size:
3423 740 707 690 57 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4234 4608 4754 387
Size: 1064 138 2645 374 146 1280
Fragments arranged by size:
2645 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2041 2579 3710 4307 5094 5362 1239
Size: 393 409 538 1131 597 787 268 1524
Fragments arranged by size:
1524 1131 787 597 538 409 393 268
EcoRI G'AATT_C
Cuts at: 2956 3334 2956
Size: 378 5269
FspI TGC'GCA
Cuts at: 4153 4153
Size: 5647
HaeII r_GCGC'y
Cuts at: 1678 2600 4794 1678
Size: 922 2194 2531
Fragments arranged by size:
2531 2194 922
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4288 4320 4362 4394 1373
Size: 32 135 32 2716 32 42 32 2626
Fragments arranged by size:
2716 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2991 3237 669
Size: 988 281 1053 246 3079
Fragments arranged by size:
3079 1053 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5647
HpaI GTT'AAC
Cuts at: 3237 3237
Size: 5647
KpnI G_GTAC'C
Cuts at: 2972 2972
Size: 5647
MfeI C'AATT_G
Cuts at: 3246 3246
Size: 5647
MluI A'CGCG_T
Cuts at: 5523 5523
Size: 5647
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4761 4945 1305
Size: 3456 184 2007
Fragments arranged by size:
3456 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3825
Fragments arranged by size:
3825 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2045 2321 2744 2780 3605 3964 4123
Size: 648 879 276 423 36 825 359 159
Cuts at: 4123 518
Size: 2042
Fragments arranged by size:
2042 879 825 648 423 359 276 159
36
MspA1I CmG'CkG
Cuts at: 1606 3063 3740 4888 5133 1606
Size: 1457 677 1148 245 2120
Fragments arranged by size:
2120 1457 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2226 513
Size: 552 939 222 3934
Fragments arranged by size:
3934 939 552 222
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5647
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5647
NotI GC'GGCC_GC
Cuts at: 3094 3094
Size: 5647
NspI r_CATG'y
Cuts at: 1132 1229 2689 3005 4550 1132
Size: 97 1460 316 1545 2229
Fragments arranged by size:
2229 1545 1460 316 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5647
PciI A'CATG_T
Cuts at: 4546 4546
Size: 5647
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5647
PmeI GTTT'AAAC
Cuts at: 2951 2951
Size: 5647
PmlI CAC'GTG
Cuts at: 2665 2665
Size: 5647
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3716 3748 5229 5261 3716
Size: 32 1481 32 4102
Fragments arranged by size:
4102 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5647
PsiI TTA'TAA
Cuts at: 3217 3217
Size: 5647
PstI C_TGCA'G
Cuts at: 830 2397 2999 830
Size: 1567 602 3478
Fragments arranged by size:
3478 1567 602
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5647
SalI G'TCGA_C
Cuts at: 1936 2989 1936
Size: 1053 4594
SbfI CC_TGCA'GG
Cuts at: 2999 2999
Size: 5647
ScaI AGT'ACT
Cuts at: 3895 5607 3895
Size: 1712 3935
SfcI C'TryA_G
Cuts at: 826 1045 2393 2836 2872 2995 4130 4811 5002
Size: 219 1348 443 36 123 1135 681 191
Cuts at: 5002 826
Size: 1471
Fragments arranged by size:
1471 1348 1135 681 443 219 191 123
36
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5647
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5647
SmaI CCC'GGG
Cuts at: 1872 2974 1872
Size: 1102 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3751 4652 4914 820
Size: 197 926 1808 901 262 1553
Fragments arranged by size:
1808 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3438 493
Size: 2945 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5647
SphI G_CATG'C
Cuts at: 1132 2689 3005 1132
Size: 1557 316 3774
Fragments arranged by size:
3774 1557 316
SspI AAT'ATT
Cuts at: 5 52 2307 3571 5
Size: 47 2255 1264 2081
Fragments arranged by size:
2255 2081 1264 47
StuI AGG'CCT
Cuts at: 1517 2118 1517
Size: 601 5046
StyI C'CwwG_G
Cuts at: 513 1065 2004 2226 3086 513
Size: 552 939 222 860 3074
Fragments arranged by size:
3074 939 860 552 222
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2191 2315 2440 3674 3691 3844 4029
Size: 1255 611 124 125 1234 17 153 185
Cuts at: 4029 4474 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1234 611 445 185 153 125
124 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 2404 2778
Size: 274 80 33 51 1443 204 223 374
Cuts at: 2778 3893 5605 96
Size: 1115 1712 138
Fragments arranged by size:
1712 1443 1115 374 274 223 204 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2064 2096 5607 5639 2064
Size: 32 3511 32 2072
Fragments arranged by size:
3511 2072 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5647
XbaI T'CTAG_A
Cuts at: 2983 2983
Size: 5647
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2242 2242
Size: 5647
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5647
XmaI C'CCGG_G
Cuts at: 1870 2972 1870
Size: 1102 4545
XmnI GAAnn'nnTTC
Cuts at: 3776 3776
Size: 5647
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3455 276
Size: 53 83 186 936 1921 2468
Fragments arranged by size:
2468 1921 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI