Restriction map for
pFN21AE6002
(Circular) (Six-base) MAPSORT of: pFN21AE6002.seq Check: 1276 from: 1 to: 5647
pFN21AE6002 5647 bp
With 182 enzymes: SgfI *
December 8, 2014 13:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3457 278
Size: 53 83 186 936 1921 2468
Fragments arranged by size:
2468 1921 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2892 2990 1937
Size: 955 98 4594
Fragments arranged by size:
4594 955 98
Acc65I G'GTAC_C
Cuts at: 2968 2968
Size: 5647
AclI AA'CG_TT
Cuts at: 1563 3774 4147 1563
Size: 2211 373 3063
Fragments arranged by size:
3063 2211 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3710 5094 5362 1632
Size: 2078 1384 268 1917
Fragments arranged by size:
2078 1917 1384 268
AfeI AGC'GCT
Cuts at: 2177 2177
Size: 5647
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5450
AflIII A'CryG_T
Cuts at: 1203 4546 5523 1203
Size: 3343 977 1327
Fragments arranged by size:
3343 1327 977
AgeI A'CCGG_T
Cuts at: 4478 4478
Size: 5647
AhdI GACnn_n'nnGTC
Cuts at: 4376 4376
Size: 5647
AleI CACnn'nnGTG
Cuts at: 1166 2754 1166
Size: 1588 4059
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5647
AlwNI CAG_nnn'CTG
Cuts at: 3390 4962 3390
Size: 1572 4075
ApaLI G'TGCA_C
Cuts at: 3704 4860 3704
Size: 1156 4491
ApoI r'AATT_y
Cuts at: 1493 2956 3182 3334 5443 1493
Size: 1463 226 152 2109 1697
Fragments arranged by size:
2109 1697 1463 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4201 160
Size: 872 3169 1606
Fragments arranged by size:
3169 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5647
AvaI C'yCGr_G
Cuts at: 1870 1943 2137 2348 2972 1870
Size: 73 194 211 624 4545
Fragments arranged by size:
4545 624 211 194 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2180 2213 2180
Size: 33 5614
BamHI G'GATC_C
Cuts at: 2015 2977 3344 2015
Size: 962 367 4318
Fragments arranged by size:
4318 962 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2821 2968 4423 618
Size: 325 217 627 1034 147 1455 1842
Fragments arranged by size:
1842 1455 1034 627 325 217 147
BanII G_rGCy'C
Cuts at: 1391 2966 1391
Size: 1575 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2909 928
Size: 971 1010 3666
Fragments arranged by size:
3666 1010 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3838 3872 1903
Size: 34 1901 34 3678
Fragments arranged by size:
3678 1901 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3539 4749 3539
Size: 1210 4437
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5647
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2711 4258 136
Size: 107 122 71 631 605 1039 1547 1525
Fragments arranged by size:
1547 1525 1039 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5642 5642
Size: 5647
BlpI GC'TnA_GC
Cuts at: 2316 3064 2316
Size: 748 4899
Bme1580I G_kGCm'C
Cuts at: 1790 3708 4864 1790
Size: 1918 1156 2573
Fragments arranged by size:
2573 1918 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5647
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2528 3378 4336
Size: 416 53 83 399 24 1111 850 958
Cuts at: 4336 442
Size: 1753
Fragments arranged by size:
1753 1111 958 850 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5647
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2099 2692 4307 1239
Size: 860 593 1615 2579
Fragments arranged by size:
2579 1615 860 593
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3772 4637 4935 3772
Size: 865 298 4484
Fragments arranged by size:
4484 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2240 2464 4310 882
Size: 617 741 224 1846 2219
Fragments arranged by size:
2219 1846 741 617 224
BsaAI yAC'GTr
Cuts at: 493 1204 3438 493
Size: 711 2234 2702
Fragments arranged by size:
2702 2234 711
BsaBI GATnn'nnATC
Cuts at: 2102 5641 2102
Size: 3539 2108
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3454 3836 4471 275
Size: 53 83 186 936 1921 382 635 1451
Fragments arranged by size:
1921 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4080 4478 4752 4899 5310 4080
Size: 398 274 147 411 4417
Fragments arranged by size:
4417 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2324 2354 1178
Size: 30 165 30 921 30 4471
Fragments arranged by size:
4471 921 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5647
BseYI C'CCAG_C
Cuts at: 4850 5346 4850
Size: 496 5151
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2131 1163
Size: 573 395 4679
Fragments arranged by size:
4679 573 395
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3097 3858 4886 1540
Size: 275 186 1096 761 1028 2301
Fragments arranged by size:
2301 1096 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2287 3708 3793 4864 2287
Size: 1421 85 1071 3070
Fragments arranged by size:
3070 1421 1071 85
BsmI GAATG_Cn'
Cuts at: 3158 3251 3158
Size: 93 5554
BsmBI CGTCTCn'nnnn_
Cuts at: 2635 2635
Size: 5647
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2287 2966 3708 3793 4864 1391
Size: 399 497 679 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 679 497 399 85
BspEI T'CCGG_A
Cuts at: 5310 5310
Size: 5647
BspHI T'CATG_A
Cuts at: 2185 3536 5266 2185
Size: 1351 1730 2566
Fragments arranged by size:
2566 1730 1351
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3002 844
Size: 2158 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3534 1394
Size: 429 1711 3507
Fragments arranged by size:
3507 1711 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4142 4316 65
Size: 1183 2894 174 1396
Fragments arranged by size:
2894 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4291 4478 1700
Size: 110 2481 187 2869
Fragments arranged by size:
2869 2481 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5647
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5647
BssSI C'ACGA_G
Cuts at: 2286 3707 4719 2286
Size: 1421 1012 3214
Fragments arranged by size:
3214 1421 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5647
BstBI TT'CG_AA
Cuts at: 4451 4451
Size: 5647
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5647
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2810 2011
Size: 799 4848
BstYI r'GATC_y
Cuts at: 1972 2015 2097 2913 2977 3344 3729 3746 5198
Size: 43 82 816 64 367 385 17 1452
Cuts at: 5198 5284 5296 5642 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 816 385 367 346 86 82
64 43 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5095
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2686 2789 487
Size: 683 56 1460 103 3345
Fragments arranged by size:
3345 1460 683 103 56
BtsI GCAGTG_nn'
Cuts at: 2332 2651 3163 3957 3977 2332
Size: 319 512 794 20 4002
Fragments arranged by size:
4002 794 512 319 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5647
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5612
DraI TTT'AAA
Cuts at: 2951 2951
Size: 5647
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4654 809
Size: 507 3338 1802
Fragments arranged by size:
3338 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3094 3983 5612
Size: 54 1425 50 275 18 1264 889 1629
Cuts at: 5612 8
Size: 43
Fragments arranged by size:
1629 1425 1264 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3094 1537
Size: 275 1282 4090
Fragments arranged by size:
4090 1282 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2207 2669 2774 3577 1353
Size: 854 462 105 803 3423
Fragments arranged by size:
3423 854 803 462 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4234 4608 4754 387
Size: 1064 138 2645 374 146 1280
Fragments arranged by size:
2645 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2099 2692 3710 4307 5094 5362 1239
Size: 393 467 593 1018 597 787 268 1524
Fragments arranged by size:
1524 1018 787 597 593 467 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2834 2834
Size: 5647
EcoO109I rG'GnC_Cy
Cuts at: 2728 2728
Size: 5647
EcoRI G'AATT_C
Cuts at: 2956 3334 2956
Size: 378 5269
FspI TGC'GCA
Cuts at: 2687 4153 2687
Size: 1466 4181
HaeII r_GCGC'y
Cuts at: 1678 2179 4794 1678
Size: 501 2615 2531
Fragments arranged by size:
2615 2531 501
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2905 2937 4288 4320 4362
Size: 32 135 32 1333 32 1351 32 42
Cuts at: 4362 4394 1373
Size: 32 2626
Fragments arranged by size:
2626 1351 1333 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2991 3237 669
Size: 988 281 1053 246 3079
Fragments arranged by size:
3079 1053 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5647
HpaI GTT'AAC
Cuts at: 3237 3237
Size: 5647
KpnI G_GTAC'C
Cuts at: 2972 2972
Size: 5647
MfeI C'AATT_G
Cuts at: 3246 3246
Size: 5647
MluI A'CGCG_T
Cuts at: 5523 5523
Size: 5647
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2260 4761 4945 1305
Size: 955 2501 184 2007
Fragments arranged by size:
2501 2007 955 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3825
Fragments arranged by size:
3825 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2754 2780 3605 3964 4123 518
Size: 648 1588 26 825 359 159 2042
Fragments arranged by size:
2042 1588 825 648 359 159 26
MspA1I CmG'CkG
Cuts at: 1606 3063 3740 4888 5133 1606
Size: 1457 677 1148 245 2120
Fragments arranged by size:
2120 1457 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5095
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5647
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5647
NotI GC'GGCC_GC
Cuts at: 3094 3094
Size: 5647
NspI r_CATG'y
Cuts at: 1132 1229 2089 2620 2936 3005 4550 1132
Size: 97 860 531 316 69 1545 2229
Fragments arranged by size:
2229 1545 860 531 316 97 69
PasI CC'CwG_GG
Cuts at: 1348 2011 2020 2072 1348
Size: 663 9 52 4923
Fragments arranged by size:
4923 663 52 9
PciI A'CATG_T
Cuts at: 4546 4546
Size: 5647
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5647
PfoI T'CCnGG_A
Cuts at: 2916 2916
Size: 5647
PmeI GTTT'AAAC
Cuts at: 2951 2951
Size: 5647
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3716 3748 5229 5261 3716
Size: 32 1481 32 4102
Fragments arranged by size:
4102 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2232 1613
Size: 619 5028
PsiI TTA'TAA
Cuts at: 3217 3217
Size: 5647
PspXI vC'TCGA_Gb
Cuts at: 2348 2348
Size: 5647
PstI C_TGCA'G
Cuts at: 830 2999 830
Size: 2169 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5647
SalI G'TCGA_C
Cuts at: 1936 2989 1936
Size: 1053 4594
SapI GCTCTTCn'nnn_
Cuts at: 2774 2774
Size: 5647
SbfI CC_TGCA'GG
Cuts at: 2999 2999
Size: 5647
ScaI AGT'ACT
Cuts at: 3895 5607 3895
Size: 1712 3935
SexAI A'CCwGG_T
Cuts at: 2829 2829
Size: 5647
SfcI C'TryA_G
Cuts at: 826 1045 2256 2634 2893 2995 4130 4811 5002
Size: 219 1211 378 259 102 1135 681 191
Cuts at: 5002 826
Size: 1471
Fragments arranged by size:
1471 1211 1135 681 378 259 219 191
102
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5647
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5647
SmaI CCC'GGG
Cuts at: 1872 2139 2974 1872
Size: 267 835 4545
Fragments arranged by size:
4545 835 267
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2348 3751 4652 4914 820
Size: 197 926 405 1403 901 262 1553
Fragments arranged by size:
1553 1403 926 901 405 262 197
SnaBI TAC'GTA
Cuts at: 493 3438 493
Size: 2945 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5647
SphI G_CATG'C
Cuts at: 1132 2089 2936 3005 1132
Size: 957 847 69 3774
Fragments arranged by size:
3774 957 847 69
SspI AAT'ATT
Cuts at: 5 52 3571 5
Size: 47 3519 2081
Fragments arranged by size:
3519 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5647
StyI C'CwwG_G
Cuts at: 513 1065 3086 513
Size: 552 2021 3074
Fragments arranged by size:
3074 2021 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3674 3691 3844 4029 4474 325
Size: 1255 2094 17 153 185 445 1498
Fragments arranged by size:
2094 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3893 5605 96
Size: 274 80 33 51 1443 1916 1712 138
Fragments arranged by size:
1916 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2324 2356 2402 2434 2643 2675 5607 5639 2324
Size: 32 46 32 209 32 2932 32 2332
Fragments arranged by size:
2932 2332 209 46 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5647
XbaI T'CTAG_A
Cuts at: 2983 2983
Size: 5647
XhoI C'TCGA_G
Cuts at: 1943 2348 1943
Size: 405 5242
XmaI C'CCGG_G
Cuts at: 1870 2137 2972 1870
Size: 267 835 4545
Fragments arranged by size:
4545 835 267
XmnI GAAnn'nnTTC
Cuts at: 3776 3776
Size: 5647
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3455 276
Size: 53 83 186 936 1921 2468
Fragments arranged by size:
2468 1921 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspXI PstI PvuI SalI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PsrI PvuII RsrII
SacI SacII SanDI SfiI SfoI SrfI SwaI XcmI