Restriction map for
pFN21AE6001
(Circular) (Six-base) MAPSORT of: pFN21AE6001.seq Check: 7043 from: 1 to: 5647
pFN21AE6001 5647 bp
With 182 enzymes: SgfI *
December 8, 2014 13:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2732 2732
Size: 5647
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3457 278
Size: 53 83 186 936 1921 2468
Fragments arranged by size:
2468 1921 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2990 1937
Size: 1053 4594
Acc65I G'GTAC_C
Cuts at: 2968 2968
Size: 5647
AclI AA'CG_TT
Cuts at: 1563 3774 4147 1563
Size: 2211 373 3063
Fragments arranged by size:
3063 2211 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3710 5094 5362 1632
Size: 2078 1384 268 1917
Fragments arranged by size:
2078 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5450
AflIII A'CryG_T
Cuts at: 1203 4546 5523 1203
Size: 3343 977 1327
Fragments arranged by size:
3343 1327 977
AgeI A'CCGG_T
Cuts at: 4478 4478
Size: 5647
AhdI GACnn_n'nnGTC
Cuts at: 4376 4376
Size: 5647
AleI CACnn'nnGTG
Cuts at: 1166 2027 2378 2843 1166
Size: 861 351 465 3970
Fragments arranged by size:
3970 861 465 351
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5647
AlwNI CAG_nnn'CTG
Cuts at: 2248 2392 3390 4962 2248
Size: 144 998 1572 2933
Fragments arranged by size:
2933 1572 998 144
ApaLI G'TGCA_C
Cuts at: 3704 4860 3704
Size: 1156 4491
ApoI r'AATT_y
Cuts at: 1493 2956 3182 3334 5443 1493
Size: 1463 226 152 2109 1697
Fragments arranged by size:
2109 1697 1463 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4201 160
Size: 872 3169 1606
Fragments arranged by size:
3169 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5647
AvaI C'yCGr_G
Cuts at: 1870 1943 2972 1870
Size: 73 1029 4545
Fragments arranged by size:
4545 1029 73
BamHI G'GATC_C
Cuts at: 2977 3344 2977
Size: 367 5280
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2762 2968 4423 618
Size: 325 217 627 975 206 1455 1842
Fragments arranged by size:
1842 1455 975 627 325 217 206
BanII G_rGCy'C
Cuts at: 1391 2966 1391
Size: 1575 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2947 928
Size: 971 1048 3628
Fragments arranged by size:
3628 1048 971
BbvCI CC'TCA_GC
Cuts at: 2192 2192
Size: 5647
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3838 3872 1903
Size: 34 1901 34 3678
Fragments arranged by size:
3678 1901 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2662 3539 4749 2662
Size: 877 1210 3560
Fragments arranged by size:
3560 1210 877
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5647
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2357 4258 136
Size: 107 122 71 631 605 685 1901 1525
Fragments arranged by size:
1901 1525 685 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5642 5642
Size: 5647
BlpI GC'TnA_GC
Cuts at: 3064 3064
Size: 5647
Bme1580I G_kGCm'C
Cuts at: 1790 3708 4864 1790
Size: 1918 1156 2573
Fragments arranged by size:
2573 1918 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5647
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2451 3378 4336
Size: 416 53 83 399 24 1034 927 958
Cuts at: 4336 442
Size: 1753
Fragments arranged by size:
1753 1034 958 927 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5647
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2097 2097
Size: 5647
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2051 2718 4307 1239
Size: 812 667 1589 2579
Fragments arranged by size:
2579 1589 812 667
Bpu10I CC'TnA_GC
Cuts at: 2192 2918 2192
Size: 726 4921
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3772 4637 4935 3772
Size: 865 298 4484
Fragments arranged by size:
4484 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4310 882
Size: 617 2811 2219
Fragments arranged by size:
2811 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3438 493
Size: 711 2234 2702
Fragments arranged by size:
2702 2234 711
BsaBI GATnn'nnATC
Cuts at: 5641 5641
Size: 5647
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3454 3836 4471 275
Size: 53 83 186 936 1921 382 635 1451
Fragments arranged by size:
1921 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4080 4478 4752 4899 5310 4080
Size: 398 274 147 411 4417
Fragments arranged by size:
4417 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2805 2835 1178
Size: 30 165 30 1402 30 3990
Fragments arranged by size:
3990 1402 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2541 1186
Size: 1355 4292
BseYI C'CCAG_C
Cuts at: 2427 4850 5346 2427
Size: 2423 496 2728
Fragments arranged by size:
2728 2423 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2316 1163
Size: 573 580 4494
Fragments arranged by size:
4494 580 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2161 2379 3097 3858 4886 1540
Size: 275 186 160 218 718 761 1028 2301
Fragments arranged by size:
2301 1028 761 718 275 218 186 160
BsiHKAI G_wGCw'C
Cuts at: 2268 2835 3708 3793 4864 2268
Size: 567 873 85 1071 3051
Fragments arranged by size:
3051 1071 873 567 85
BsmI GAATG_Cn'
Cuts at: 3158 3251 3158
Size: 93 5554
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2268 2835 2966 3708 3793 4864 1391
Size: 399 478 567 131 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 567 478 399 131 85
BspEI T'CCGG_A
Cuts at: 5310 5310
Size: 5647
BspHI T'CATG_A
Cuts at: 3536 5266 3536
Size: 1730 3917
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2732 2790 3002 844
Size: 1888 58 212 3489
Fragments arranged by size:
3489 1888 212 58
BsrBI CCG'CTC
Cuts at: 1394 1823 3534 1394
Size: 429 1711 3507
Fragments arranged by size:
3507 1711 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4142 4316 65
Size: 1183 2894 174 1396
Fragments arranged by size:
2894 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4291 4478 1700
Size: 110 2481 187 2869
Fragments arranged by size:
2869 2481 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5647
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5647
BssSI C'ACGA_G
Cuts at: 3707 4719 3707
Size: 1012 4635
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5647
BstBI TT'CG_AA
Cuts at: 2479 4451 2479
Size: 1972 3675
BstEII G'GTnAC_C
Cuts at: 1184 2845 1184
Size: 1661 3986
BstXI CCAn_nnnn'nTGG
Cuts at: 2696 2696
Size: 5647
BstYI r'GATC_y
Cuts at: 1972 2685 2977 3344 3729 3746 5198 5284 5296
Size: 713 292 367 385 17 1452 86 12
Cuts at: 5296 5642 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 713 385 367 346 292 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2152 513
Size: 552 939 148 4008
Fragments arranged by size:
4008 939 552 148
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4908
Fragments arranged by size:
4908 683 56
BtsI GCAGTG_nn'
Cuts at: 2739 3163 3957 3977 2739
Size: 424 794 20 4409
Fragments arranged by size:
4409 794 424 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5647
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5612
DraI TTT'AAA
Cuts at: 2951 2951
Size: 5647
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4654 809
Size: 507 3338 1802
Fragments arranged by size:
3338 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2349 2376 2673
Size: 54 1425 50 275 18 519 27 297
Cuts at: 2673 3094 3983 5612 8
Size: 421 889 1629 43
Fragments arranged by size:
1629 1425 889 519 421 297 275 54
50 43 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 2376 3094 1537
Size: 275 564 718 4090
Fragments arranged by size:
4090 718 564 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2090 2318 3577 1353
Size: 737 228 1259 3423
Fragments arranged by size:
3423 1259 737 228
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2218 4234 4608 4754 387
Size: 1064 138 629 2016 374 146 1280
Fragments arranged by size:
2016 1280 1064 629 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2051 2718 3710 4307 5094 5362 1239
Size: 393 419 667 992 597 787 268 1524
Fragments arranged by size:
1524 992 787 667 597 419 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2431 2431
Size: 5647
EcoRI G'AATT_C
Cuts at: 2956 3334 2956
Size: 378 5269
FspI TGC'GCA
Cuts at: 4153 4153
Size: 5647
HaeII r_GCGC'y
Cuts at: 1678 4794 1678
Size: 3116 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2092 2124 4288 4320 4362
Size: 32 135 32 520 32 2164 32 42
Cuts at: 4362 4394 1373
Size: 32 2626
Fragments arranged by size:
2626 2164 520 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2991 3237 669
Size: 988 281 1053 246 3079
Fragments arranged by size:
3079 1053 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5647
HpaI GTT'AAC
Cuts at: 3237 3237
Size: 5647
KpnI G_GTAC'C
Cuts at: 2972 2972
Size: 5647
MfeI C'AATT_G
Cuts at: 3246 3246
Size: 5647
MluI A'CGCG_T
Cuts at: 5523 5523
Size: 5647
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4761 4945 1305
Size: 3456 184 2007
Fragments arranged by size:
3456 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2351 2675 10
Size: 54 1425 343 519 324 2982
Fragments arranged by size:
2982 1425 519 343 324 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2027 2135 2177 2378 2774 2843 3605
Size: 648 861 108 42 201 396 69 762
Cuts at: 3605 3964 4123 518
Size: 359 159 2042
Fragments arranged by size:
2042 861 762 648 396 359 201 159
108 69 42
MspA1I CmG'CkG
Cuts at: 1606 2427 3063 3740 4888 5133 1606
Size: 821 636 677 1148 245 2120
Fragments arranged by size:
2120 1148 821 677 636 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4156
Fragments arranged by size:
4156 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5647
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5647
NotI GC'GGCC_GC
Cuts at: 3094 3094
Size: 5647
NspI r_CATG'y
Cuts at: 1132 1229 3005 4550 1132
Size: 97 1776 1545 2229
Fragments arranged by size:
2229 1776 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5647
PciI A'CATG_T
Cuts at: 4546 4546
Size: 5647
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5647
PmeI GTTT'AAAC
Cuts at: 2951 2951
Size: 5647
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3716 3748 5229 5261 3716
Size: 32 1481 32 4102
Fragments arranged by size:
4102 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5647
PsiI TTA'TAA
Cuts at: 3217 3217
Size: 5647
PstI C_TGCA'G
Cuts at: 830 2685 2999 830
Size: 1855 314 3478
Fragments arranged by size:
3478 1855 314
PvuI CG_AT'CG
Cuts at: 2001 2161 2001
Size: 160 5487
SalI G'TCGA_C
Cuts at: 1936 2989 1936
Size: 1053 4594
SbfI CC_TGCA'GG
Cuts at: 2999 2999
Size: 5647
ScaI AGT'ACT
Cuts at: 3895 5607 3895
Size: 1712 3935
SfcI C'TryA_G
Cuts at: 826 1045 2681 2872 2995 4130 4811 5002 826
Size: 219 1636 191 123 1135 681 191 1471
Fragments arranged by size:
1636 1471 1135 681 219 191 191 123
SfiI GGCCn_nnn'nGGCC
Cuts at: 2357 2357
Size: 5647
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5647
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5647
SmaI CCC'GGG
Cuts at: 1872 2974 1872
Size: 1102 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3751 4652 4914 820
Size: 197 926 1808 901 262 1553
Fragments arranged by size:
1808 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3438 493
Size: 2945 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5647
SphI G_CATG'C
Cuts at: 1132 3005 1132
Size: 1873 3774
SspI AAT'ATT
Cuts at: 5 52 3571 5
Size: 47 3519 2081
Fragments arranged by size:
3519 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5647
StyI C'CwwG_G
Cuts at: 513 1065 2004 2205 3086 513
Size: 552 939 201 881 3074
Fragments arranged by size:
3074 939 881 552 201
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3674 3691 3844 4029 4474 325
Size: 1255 2094 17 153 185 445 1498
Fragments arranged by size:
2094 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 3893 5605
Size: 274 80 33 51 1443 204 1712 1712
Cuts at: 5605 96
Size: 138
Fragments arranged by size:
1712 1712 1443 274 204 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2805 2837 5607 5639 2805
Size: 32 2770 32 2813
Fragments arranged by size:
2813 2770 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5647
XbaI T'CTAG_A
Cuts at: 2983 2983
Size: 5647
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5647
XmaI C'CCGG_G
Cuts at: 1870 2972 1870
Size: 1102 4545
XmnI GAAnn'nnTTC
Cuts at: 3776 3776
Size: 5647
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3455 276
Size: 53 83 186 936 1921 2468
Fragments arranged by size:
2468 1921 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SalI SbfI ScaI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfoI
SrfI SwaI XcmI