Restriction map for
pFN21AE5988
(Circular) (Six-base) MAPSORT of: pFN21AE5988.seq Check: 9751 from: 1 to: 5644
pFN21AE5988 5644 bp
With 182 enzymes: SgfI *
December 8, 2014 13:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2517 3454 278
Size: 53 83 186 936 981 937 2468
Fragments arranged by size:
2468 981 937 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2987 1937
Size: 1050 4594
Acc65I G'GTAC_C
Cuts at: 2467 2965 2467
Size: 498 5146
AclI AA'CG_TT
Cuts at: 1563 3771 4144 1563
Size: 2208 373 3063
Fragments arranged by size:
3063 2208 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2817 3707 5091 5359 1632
Size: 1185 890 1384 268 1917
Fragments arranged by size:
1917 1384 1185 890 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5447
AflIII A'CryG_T
Cuts at: 1203 4543 5520 1203
Size: 3340 977 1327
Fragments arranged by size:
3340 1327 977
AgeI A'CCGG_T
Cuts at: 4475 4475
Size: 5644
AhdI GACnn_n'nnGTC
Cuts at: 4373 4373
Size: 5644
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5644
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5644
AlwNI CAG_nnn'CTG
Cuts at: 2697 2920 3387 4959 2697
Size: 223 467 1572 3382
Fragments arranged by size:
3382 1572 467 223
ApaLI G'TGCA_C
Cuts at: 3701 4857 3701
Size: 1156 4488
ApoI r'AATT_y
Cuts at: 1493 2030 2585 2953 3179 3331 5440 1493
Size: 537 555 368 226 152 2109 1697
Fragments arranged by size:
2109 1697 555 537 368 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4198 160
Size: 872 3166 1606
Fragments arranged by size:
3166 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5644
AvaI C'yCGr_G
Cuts at: 1870 1943 2484 2969 1870
Size: 73 541 485 4545
Fragments arranged by size:
4545 541 485 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2743 2776 2743
Size: 33 5611
BamHI G'GATC_C
Cuts at: 2679 2974 3341 2679
Size: 295 367 4982
Fragments arranged by size:
4982 367 295
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2467 2965 4420 618
Size: 325 217 627 680 498 1455 1842
Fragments arranged by size:
1842 1455 680 627 498 325 217
BanII G_rGCy'C
Cuts at: 1391 2491 2963 1391
Size: 1100 472 4072
Fragments arranged by size:
4072 1100 472
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2734 928
Size: 971 835 3838
Fragments arranged by size:
3838 971 835
BbvCI CC'TCA_GC
Cuts at: 2621 2621
Size: 5644
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3835 3869 1903
Size: 34 1898 34 3678
Fragments arranged by size:
3678 1898 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3536 4746 3536
Size: 1210 4434
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5644
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4255 136
Size: 107 122 71 631 605 2583 1525
Fragments arranged by size:
2583 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2208 5639 2208
Size: 3431 2213
BlpI GC'TnA_GC
Cuts at: 3061 3061
Size: 5644
Bme1580I G_kGCm'C
Cuts at: 1790 2235 3705 4861 1790
Size: 445 1470 1156 2573
Fragments arranged by size:
2573 1470 1156 445
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5644
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3375 4333 442
Size: 416 53 83 399 24 1958 958 1753
Fragments arranged by size:
1958 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5644
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2225 4304 1239
Size: 986 2079 2579
Fragments arranged by size:
2579 2079 986
Bpu10I CC'TnA_GC
Cuts at: 2621 2621
Size: 5644
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3769 4634 4932 3769
Size: 865 298 4481
Fragments arranged by size:
4481 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2730 4307 882
Size: 617 1231 1577 2219
Fragments arranged by size:
2219 1577 1231 617
BsaAI yAC'GTr
Cuts at: 493 1204 3435 493
Size: 711 2231 2702
Fragments arranged by size:
2702 2231 711
BsaBI GATnn'nnATC
Cuts at: 5638 5638
Size: 5644
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2514 3451 3833 4468
Size: 53 83 186 936 981 937 382 635
Cuts at: 4468 275
Size: 1451
Fragments arranged by size:
1451 981 937 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4077 4475 4749 4896 5307 4077
Size: 398 274 147 411 4414
Fragments arranged by size:
4414 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5419
Fragments arranged by size:
5419 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2041 1186
Size: 855 4789
BseYI C'CCAG_C
Cuts at: 2432 4847 5343 2432
Size: 2415 496 2733
Fragments arranged by size:
2733 2415 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5071
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3094 3855 4883 1540
Size: 275 186 1093 761 1028 2301
Fragments arranged by size:
2301 1093 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2491 3705 3790 4861 2491
Size: 1214 85 1071 3274
Fragments arranged by size:
3274 1214 1071 85
BsmI GAATG_Cn'
Cuts at: 3155 3248 3155
Size: 93 5551
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2235 2491 2963 3705 3790 4861 1391
Size: 399 445 256 472 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 472 445 399 256 85
BspEI T'CCGG_A
Cuts at: 5307 5307
Size: 5644
BspHI T'CATG_A
Cuts at: 3533 5263 3533
Size: 1730 3914
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2999 844
Size: 2155 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3531 1394
Size: 429 1708 3507
Fragments arranged by size:
3507 1708 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4139 4313 65
Size: 1183 2891 174 1396
Fragments arranged by size:
2891 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4288 4475 1700
Size: 110 2478 187 2869
Fragments arranged by size:
2869 2478 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5644
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5644
BssSI C'ACGA_G
Cuts at: 3704 4716 3704
Size: 1012 4632
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5644
BstBI TT'CG_AA
Cuts at: 4448 4448
Size: 5644
BstEII G'GTnAC_C
Cuts at: 1184 2215 2635 1184
Size: 1031 420 4193
Fragments arranged by size:
4193 1031 420
BstXI CCAn_nnnn'nTGG
Cuts at: 2432 2432
Size: 5644
BstYI r'GATC_y
Cuts at: 1972 2208 2679 2974 3341 3726 3743 5195 5281
Size: 236 471 295 367 385 17 1452 86
Cuts at: 5281 5293 5639 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 471 385 367 346 295 236
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2463 2506 513
Size: 552 939 459 43 3651
Fragments arranged by size:
3651 939 552 459 43
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4905
Fragments arranged by size:
4905 683 56
BtsI GCAGTG_nn'
Cuts at: 2235 2384 3160 3954 3974 2235
Size: 149 776 794 20 3905
Fragments arranged by size:
3905 794 776 149 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5644
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5609
DraI TTT'AAA
Cuts at: 2948 2948
Size: 5644
DraIII CAC_nnn'GTG
Cuts at: 2800 2800
Size: 5644
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2255 4651 809
Size: 507 939 2396 1802
Fragments arranged by size:
2396 1802 939 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2370 3091 3980
Size: 54 1425 50 275 18 540 721 889
Cuts at: 3980 5609 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 721 540 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3091 1537
Size: 275 1279 4090
Fragments arranged by size:
4090 1279 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3574 1353
Size: 2221 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4231 4605 4751 387
Size: 1064 138 2642 374 146 1280
Fragments arranged by size:
2642 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2489 2489
Size: 5644
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2225 2817 3707 4304 5091 5359 1239
Size: 393 593 592 890 597 787 268 1524
Fragments arranged by size:
1524 890 787 597 593 592 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2933 2933
Size: 5644
EcoO109I rG'GnC_Cy
Cuts at: 2071 2071
Size: 5644
EcoRI G'AATT_C
Cuts at: 2585 2953 3331 2585
Size: 368 378 4898
Fragments arranged by size:
4898 378 368
FspI TGC'GCA
Cuts at: 4150 4150
Size: 5644
HaeII r_GCGC'y
Cuts at: 1678 4791 1678
Size: 3113 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4285 4317 4359 4391 1373
Size: 32 135 32 2713 32 42 32 2626
Fragments arranged by size:
2713 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2988 3234 669
Size: 988 281 1050 246 3079
Fragments arranged by size:
3079 1050 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5644
HpaI GTT'AAC
Cuts at: 3234 3234
Size: 5644
KpnI G_GTAC'C
Cuts at: 2471 2969 2471
Size: 498 5146
MfeI C'AATT_G
Cuts at: 3243 3243
Size: 5644
MluI A'CGCG_T
Cuts at: 5520 5520
Size: 5644
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2562 4758 4942 1305
Size: 1257 2196 184 2007
Fragments arranged by size:
2196 2007 1257 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2372 10
Size: 54 1425 343 540 3282
Fragments arranged by size:
3282 1425 540 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2138 2402 3602 3961 4120 518
Size: 648 972 264 1200 359 159 2042
Fragments arranged by size:
2042 1200 972 648 359 264 159
MspA1I CmG'CkG
Cuts at: 1606 2428 2697 3060 3737 4885 5130 1606
Size: 822 269 363 677 1148 245 2120
Fragments arranged by size:
2120 1148 822 677 363 269 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2463 2506 513
Size: 552 939 459 43 3651
Fragments arranged by size:
3651 939 552 459 43
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5644
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5644
NotI GC'GGCC_GC
Cuts at: 3091 3091
Size: 5644
NspI r_CATG'y
Cuts at: 1132 1229 3002 4547 1132
Size: 97 1773 1545 2229
Fragments arranged by size:
2229 1773 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2317 1348
Size: 969 4675
PciI A'CATG_T
Cuts at: 4543 4543
Size: 5644
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5644
PmeI GTTT'AAAC
Cuts at: 2948 2948
Size: 5644
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3713 3745 5226 5258 3713
Size: 32 1481 32 4099
Fragments arranged by size:
4099 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2615 1613
Size: 1002 4642
PsiI TTA'TAA
Cuts at: 3214 3214
Size: 5644
PstI C_TGCA'G
Cuts at: 830 2070 2230 2556 2996 830
Size: 1240 160 326 440 3478
Fragments arranged by size:
3478 1240 440 326 160
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5644
PvuII CAG'CTG
Cuts at: 2428 2697 2428
Size: 269 5375
SacI G_AGCT'C
Cuts at: 2491 2491
Size: 5644
SalI G'TCGA_C
Cuts at: 1936 2986 1936
Size: 1050 4594
SbfI CC_TGCA'GG
Cuts at: 2996 2996
Size: 5644
ScaI AGT'ACT
Cuts at: 3892 5604 3892
Size: 1712 3932
SfcI C'TryA_G
Cuts at: 826 1045 2066 2226 2552 2752 2900 2992 4127
Size: 219 1021 160 326 200 148 92 1135
Cuts at: 4127 4808 4999 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1021 681 326 219 200 191
160 148 92
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5644
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5644
SmaI CCC'GGG
Cuts at: 1872 2971 1872
Size: 1099 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3748 4649 4911 820
Size: 197 926 1805 901 262 1553
Fragments arranged by size:
1805 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3435 493
Size: 2942 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5644
SphI G_CATG'C
Cuts at: 1132 3002 1132
Size: 1870 3774
SspI AAT'ATT
Cuts at: 5 52 3568 5
Size: 47 3516 2081
Fragments arranged by size:
3516 2081 47
StuI AGG'CCT
Cuts at: 1517 2782 2928 1517
Size: 1265 146 4233
Fragments arranged by size:
4233 1265 146
StyI C'CwwG_G
Cuts at: 513 1065 2004 2463 2506 2823 3083 513
Size: 552 939 459 43 317 260 3074
Fragments arranged by size:
3074 939 552 459 317 260 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2171 2185 2397 3671 3688 3841 4026
Size: 1255 591 14 212 1274 17 153 185
Cuts at: 4026 4471 325
Size: 445 1498
Fragments arranged by size:
1498 1274 1255 591 445 212 185 153
17 14
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2175 3890 5602
Size: 274 80 33 51 1443 198 1715 1712
Cuts at: 5602 96
Size: 138
Fragments arranged by size:
1715 1712 1443 274 198 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5604 5636 5604
Size: 32 5612
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5644
XbaI T'CTAG_A
Cuts at: 2980 2980
Size: 5644
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5644
XmaI C'CCGG_G
Cuts at: 1870 2969 1870
Size: 1099 4545
XmnI GAAnn'nnTTC
Cuts at: 2708 3773 2708
Size: 1065 4579
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2515 3452 276
Size: 53 83 186 936 981 937 2468
Fragments arranged by size:
2468 981 937 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI