Restriction map for
pFN21AE5984
(Circular) (Six-base) MAPSORT of: pFN21AE5984.seq Check: 8088 from: 1 to: 5644
pFN21AE5984 5644 bp
With 182 enzymes: SgfI *
December 8, 2014 13:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3454 278
Size: 53 83 186 936 1918 2468
Fragments arranged by size:
2468 1918 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2987 1937
Size: 1050 4594
Acc65I G'GTAC_C
Cuts at: 2629 2965 2629
Size: 336 5308
AclI AA'CG_TT
Cuts at: 1563 3771 4144 1563
Size: 2208 373 3063
Fragments arranged by size:
3063 2208 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2701 3707 5091 5359 1632
Size: 1069 1006 1384 268 1917
Fragments arranged by size:
1917 1384 1069 1006 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5447
AflIII A'CryG_T
Cuts at: 1203 4543 5520 1203
Size: 3340 977 1327
Fragments arranged by size:
3340 1327 977
AgeI A'CCGG_T
Cuts at: 4475 4475
Size: 5644
AhdI GACnn_n'nnGTC
Cuts at: 4373 4373
Size: 5644
AleI CACnn'nnGTG
Cuts at: 1166 2840 1166
Size: 1674 3970
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5644
AlwNI CAG_nnn'CTG
Cuts at: 3387 4959 3387
Size: 1572 4072
ApaLI G'TGCA_C
Cuts at: 3701 4857 3701
Size: 1156 4488
ApoI r'AATT_y
Cuts at: 1493 2036 2158 2922 2953 3179 3331 5440 1493
Size: 543 122 764 31 226 152 2109 1697
Fragments arranged by size:
2109 1697 764 543 226 152 122 31
AseI AT'TA_AT
Cuts at: 160 1032 2619 4198 160
Size: 872 1587 1579 1606
Fragments arranged by size:
1606 1587 1579 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5644
AvaI C'yCGr_G
Cuts at: 1870 1943 2969 1870
Size: 73 1026 4545
Fragments arranged by size:
4545 1026 73
BamHI G'GATC_C
Cuts at: 2974 3341 2974
Size: 367 5277
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2629 2845 2965 4420 618
Size: 325 217 627 842 216 120 1455 1842
Fragments arranged by size:
1842 1455 842 627 325 217 216 120
BanII G_rGCy'C
Cuts at: 1391 2963 1391
Size: 1572 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4673
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3835 3869 1903
Size: 34 50 34 1814 34 3678
Fragments arranged by size:
3678 1814 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3536 4746 3536
Size: 1210 4434
BclI T'GATC_A
Cuts at: 1548 2139 1548
Size: 591 5053
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4255 136
Size: 107 122 71 631 605 337 2246 1525
Fragments arranged by size:
2246 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5639 5639
Size: 5644
BlpI GC'TnA_GC
Cuts at: 3061 3061
Size: 5644
Bme1580I G_kGCm'C
Cuts at: 1790 2850 3705 4861 1790
Size: 1060 855 1156 2573
Fragments arranged by size:
2573 1156 1060 855
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5644
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2628 3375 4333
Size: 416 53 83 399 24 1211 747 958
Cuts at: 4333 442
Size: 1753
Fragments arranged by size:
1753 1211 958 747 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5644
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2928 2928
Size: 5644
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2487 2543 4304 1239
Size: 1248 56 1761 2579
Fragments arranged by size:
2579 1761 1248 56
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2682 3769 4634 4932 2682
Size: 1087 865 298 3394
Fragments arranged by size:
3394 1087 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4307 882
Size: 617 2808 2219
Fragments arranged by size:
2808 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3435 493
Size: 711 2231 2702
Fragments arranged by size:
2702 2231 711
BsaBI GATnn'nnATC
Cuts at: 5638 5638
Size: 5644
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3451 3833 4468 275
Size: 53 83 186 936 1918 382 635 1451
Fragments arranged by size:
1918 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4077 4475 4749 4896 5307 4077
Size: 398 274 147 411 4414
Fragments arranged by size:
4414 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2538 2568 1178
Size: 30 165 30 1135 30 4254
Fragments arranged by size:
4254 1135 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2215 2546 1186
Size: 1029 331 4284
Fragments arranged by size:
4284 1029 331
BseYI C'CCAG_C
Cuts at: 4847 5343 4847
Size: 496 5148
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2554 1163
Size: 573 818 4253
Fragments arranged by size:
4253 818 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3094 3855 4883 1540
Size: 275 186 1093 761 1028 2301
Fragments arranged by size:
2301 1093 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3705 3790 4861 3705
Size: 85 1071 4488
Fragments arranged by size:
4488 1071 85
BsmI GAATG_Cn'
Cuts at: 2342 2676 2771 3155 3248 2342
Size: 334 95 384 93 4738
Fragments arranged by size:
4738 384 334 95 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2850 2963 3705 3790 4861 1391
Size: 399 1060 113 742 85 1071 2174
Fragments arranged by size:
2174 1071 1060 742 399 113 85
BspEI T'CCGG_A
Cuts at: 5307 5307
Size: 5644
BspHI T'CATG_A
Cuts at: 3533 5263 3533
Size: 1730 3914
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2999 844
Size: 2155 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3531 1394
Size: 429 1708 3507
Fragments arranged by size:
3507 1708 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2390 2739 4139 4313 65
Size: 1183 1142 349 1400 174 1396
Fragments arranged by size:
1400 1396 1183 1142 349 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4288 4475 1700
Size: 110 2478 187 2869
Fragments arranged by size:
2869 2478 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5644
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5644
BssSI C'ACGA_G
Cuts at: 3704 4716 3704
Size: 1012 4632
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5644
BstBI TT'CG_AA
Cuts at: 4448 4448
Size: 5644
BstEII G'GTnAC_C
Cuts at: 1184 2404 1184
Size: 1220 4424
BstYI r'GATC_y
Cuts at: 1972 2798 2974 3341 3726 3743 5195 5281 5293
Size: 826 176 367 385 17 1452 86 12
Cuts at: 5293 5639 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 826 385 367 346 176 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2118 2472 513
Size: 552 1053 354 3685
Fragments arranged by size:
3685 1053 552 354
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4905
Fragments arranged by size:
4905 683 56
BtsI GCAGTG_nn'
Cuts at: 2518 3160 3954 3974 2518
Size: 642 794 20 4188
Fragments arranged by size:
4188 794 642 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5644
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5609
DraI TTT'AAA
Cuts at: 2948 2948
Size: 5644
DraIII CAC_nnn'GTG
Cuts at: 2487 2487
Size: 5644
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4651 809
Size: 507 3335 1802
Fragments arranged by size:
3335 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2376 3091 3980
Size: 54 1425 50 275 18 546 715 889
Cuts at: 3980 5609 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 715 546 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3091 1537
Size: 275 1279 4090
Fragments arranged by size:
4090 1279 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2198 3574 1353
Size: 845 1376 3423
Fragments arranged by size:
3423 1376 845
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4231 4605 4751 387
Size: 1064 138 2642 374 146 1280
Fragments arranged by size:
2642 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2487 2543 2701 3707 4304 5091 5359
Size: 393 855 56 158 1006 597 787 268
Cuts at: 5359 1239
Size: 1524
Fragments arranged by size:
1524 1006 855 787 597 393 268 158
56
EcoRI G'AATT_C
Cuts at: 2953 3331 2953
Size: 378 5266
FspI TGC'GCA
Cuts at: 4150 4150
Size: 5644
HaeII r_GCGC'y
Cuts at: 1678 4791 1678
Size: 3113 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4285 4317 4359 4391 1373
Size: 32 135 32 2713 32 42 32 2626
Fragments arranged by size:
2713 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2587 2988 3234 669
Size: 988 281 649 401 246 3079
Fragments arranged by size:
3079 988 649 401 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5644
HpaI GTT'AAC
Cuts at: 3234 3234
Size: 5644
KpnI G_GTAC'C
Cuts at: 2633 2969 2633
Size: 336 5308
MfeI C'AATT_G
Cuts at: 3243 3243
Size: 5644
MluI A'CGCG_T
Cuts at: 5520 5520
Size: 5644
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4758 4942 1305
Size: 3453 184 2007
Fragments arranged by size:
3453 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2378 10
Size: 54 1425 343 546 3276
Fragments arranged by size:
3276 1425 546 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2177 2609 2840 3602 3961 4120 518
Size: 648 1011 432 231 762 359 159 2042
Fragments arranged by size:
2042 1011 762 648 432 359 231 159
MspA1I CmG'CkG
Cuts at: 1606 2305 2768 3060 3737 4885 5130 1606
Size: 699 463 292 677 1148 245 2120
Fragments arranged by size:
2120 1148 699 677 463 292 245
NcoI C'CATG_G
Cuts at: 513 1065 2118 513
Size: 552 1053 4039
Fragments arranged by size:
4039 1053 552
NdeI CA'TA_TG
Cuts at: 387 2362 387
Size: 1975 3669
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5644
NotI GC'GGCC_GC
Cuts at: 3091 3091
Size: 5644
NspI r_CATG'y
Cuts at: 1132 1229 2494 3002 4547 1132
Size: 97 1265 508 1545 2229
Fragments arranged by size:
2229 1545 1265 508 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5644
PciI A'CATG_T
Cuts at: 4543 4543
Size: 5644
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5644
PmeI GTTT'AAAC
Cuts at: 2948 2948
Size: 5644
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3713 3745 5226 5258 3713
Size: 32 1481 32 4099
Fragments arranged by size:
4099 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5644
PsiI TTA'TAA
Cuts at: 3214 3214
Size: 5644
PstI C_TGCA'G
Cuts at: 830 2513 2996 830
Size: 1683 483 3478
Fragments arranged by size:
3478 1683 483
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5644
PvuII CAG'CTG
Cuts at: 2305 2305
Size: 5644
SalI G'TCGA_C
Cuts at: 1936 2986 1936
Size: 1050 4594
SbfI CC_TGCA'GG
Cuts at: 2996 2996
Size: 5644
ScaI AGT'ACT
Cuts at: 2722 3892 5604 2722
Size: 1170 1712 2762
Fragments arranged by size:
2762 1712 1170
SfcI C'TryA_G
Cuts at: 826 1045 2509 2869 2992 4127 4808 4999 826
Size: 219 1464 360 123 1135 681 191 1471
Fragments arranged by size:
1471 1464 1135 681 360 219 191 123
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5644
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5644
SmaI CCC'GGG
Cuts at: 1872 2971 1872
Size: 1099 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2697 3748 4649 4911 820
Size: 197 926 754 1051 901 262 1553
Fragments arranged by size:
1553 1051 926 901 754 262 197
SnaBI TAC'GTA
Cuts at: 493 3435 493
Size: 2942 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5644
SphI G_CATG'C
Cuts at: 1132 2494 3002 1132
Size: 1362 508 3774
Fragments arranged by size:
3774 1362 508
SspI AAT'ATT
Cuts at: 5 52 2275 2789 3568 5
Size: 47 2223 514 779 2081
Fragments arranged by size:
2223 2081 779 514 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5644
StyI C'CwwG_G
Cuts at: 513 1065 2118 2131 3083 513
Size: 552 1053 13 952 3074
Fragments arranged by size:
3074 1053 952 552 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2141 2280 3671 3688 3841 4026 4471
Size: 1255 561 139 1391 17 153 185 445
Cuts at: 4471 325
Size: 1498
Fragments arranged by size:
1498 1391 1255 561 445 185 153 139
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 2720 3890
Size: 274 80 33 51 1443 204 539 1170
Cuts at: 3890 5602 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1170 539 274 204 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5604 5636 5604
Size: 32 5612
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5644
XbaI T'CTAG_A
Cuts at: 2980 2980
Size: 5644
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5644
XmaI C'CCGG_G
Cuts at: 1870 2969 1870
Size: 1099 4545
XmnI GAAnn'nnTTC
Cuts at: 2040 2818 3773 2040
Size: 778 955 3911
Fragments arranged by size:
3911 955 778
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3452 276
Size: 53 83 186 936 1918 2468
Fragments arranged by size:
2468 1918 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI