Restriction map for
pFN21AE5979
(Circular) (Six-base) MAPSORT of: pFN21AE5979.seq Check: 3087 from: 1 to: 5644
pFN21AE5979 5644 bp
With 182 enzymes: SgfI *
December 8, 2014 13:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3454 278
Size: 53 83 186 936 1918 2468
Fragments arranged by size:
2468 1918 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2987 1937
Size: 1050 4594
Acc65I G'GTAC_C
Cuts at: 2965 2965
Size: 5644
AclI AA'CG_TT
Cuts at: 1563 3771 4144 1563
Size: 2208 373 3063
Fragments arranged by size:
3063 2208 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2534 3707 5091 5359 1632
Size: 902 1173 1384 268 1917
Fragments arranged by size:
1917 1384 1173 902 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5447
AflIII A'CryG_T
Cuts at: 1203 2649 4543 5520 1203
Size: 1446 1894 977 1327
Fragments arranged by size:
1894 1446 1327 977
AgeI A'CCGG_T
Cuts at: 4475 4475
Size: 5644
AhdI GACnn_n'nnGTC
Cuts at: 4373 4373
Size: 5644
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5644
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5644
AlwNI CAG_nnn'CTG
Cuts at: 2418 3387 4959 2418
Size: 969 1572 3103
Fragments arranged by size:
3103 1572 969
ApaLI G'TGCA_C
Cuts at: 3701 4857 3701
Size: 1156 4488
ApoI r'AATT_y
Cuts at: 1493 2953 3179 3331 5440 1493
Size: 1460 226 152 2109 1697
Fragments arranged by size:
2109 1697 1460 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2517 4198 160
Size: 872 1485 1681 1606
Fragments arranged by size:
1681 1606 1485 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5644
AvaI C'yCGr_G
Cuts at: 1870 1943 2969 1870
Size: 73 1026 4545
Fragments arranged by size:
4545 1026 73
AvrII C'CTAG_G
Cuts at: 2254 2254
Size: 5644
BamHI G'GATC_C
Cuts at: 2974 3341 2974
Size: 367 5277
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2965 4420 618
Size: 325 217 627 1178 1455 1842
Fragments arranged by size:
1842 1455 1178 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2624 2963 1391
Size: 1233 339 4072
Fragments arranged by size:
4072 1233 339
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4673
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3835 3869 1903
Size: 34 1898 34 3678
Fragments arranged by size:
3678 1898 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2687 3536 4746 2687
Size: 849 1210 3585
Fragments arranged by size:
3585 1210 849
BclI T'GATC_A
Cuts at: 1548 2152 1548
Size: 604 5040
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4255 136
Size: 107 122 71 631 605 2583 1525
Fragments arranged by size:
2583 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5639 5639
Size: 5644
BlpI GC'TnA_GC
Cuts at: 3061 3061
Size: 5644
Bme1580I G_kGCm'C
Cuts at: 1790 3705 4861 1790
Size: 1915 1156 2573
Fragments arranged by size:
2573 1915 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5644
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2649 3375 4333
Size: 416 53 83 399 24 1232 726 958
Cuts at: 4333 442
Size: 1753
Fragments arranged by size:
1753 1232 958 726 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5644
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2035 2081 4304 1239
Size: 796 46 2223 2579
Fragments arranged by size:
2579 2223 796 46
Bpu10I CC'TnA_GC
Cuts at: 2618 2618
Size: 5644
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2890 3769 4634 4932 2890
Size: 879 865 298 3602
Fragments arranged by size:
3602 879 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2770 4307 882
Size: 617 1271 1537 2219
Fragments arranged by size:
2219 1537 1271 617
BsaAI yAC'GTr
Cuts at: 493 1204 2650 3435 493
Size: 711 1446 785 2702
Fragments arranged by size:
2702 1446 785 711
BsaBI GATnn'nnATC
Cuts at: 5638 5638
Size: 5644
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3451 3833 4468 275
Size: 53 83 186 936 1918 382 635 1451
Fragments arranged by size:
1918 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4077 4475 4749 4896 5307 4077
Size: 398 274 147 411 4414
Fragments arranged by size:
4414 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5419
Fragments arranged by size:
5419 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2588 2782 1186
Size: 1402 194 4048
Fragments arranged by size:
4048 1402 194
BseYI C'CCAG_C
Cuts at: 2050 2442 4847 5343 2050
Size: 392 2405 496 2351
Fragments arranged by size:
2405 2351 496 392
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2372 1163
Size: 573 636 4435
Fragments arranged by size:
4435 636 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3094 3855 4883 1540
Size: 275 186 1093 761 1028 2301
Fragments arranged by size:
2301 1093 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2021 2624 3705 3790 4861 2021
Size: 603 1081 85 1071 2804
Fragments arranged by size:
2804 1081 1071 603 85
BsmI GAATG_Cn'
Cuts at: 2630 3155 3248 2630
Size: 525 93 5026
Fragments arranged by size:
5026 525 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2021 2624 2963 3705 3790 4861 1391
Size: 399 231 603 339 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 603 399 339 231 85
BspEI T'CCGG_A
Cuts at: 5307 5307
Size: 5644
BspHI T'CATG_A
Cuts at: 2403 2919 3533 5263 2403
Size: 516 614 1730 2784
Fragments arranged by size:
2784 1730 614 516
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2999 844
Size: 2155 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3531 1394
Size: 429 1708 3507
Fragments arranged by size:
3507 1708 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2535 2742 4139 4313 65
Size: 1183 1287 207 1397 174 1396
Fragments arranged by size:
1397 1396 1287 1183 207 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4288 4475 1700
Size: 110 2478 187 2869
Fragments arranged by size:
2869 2478 187 110
BsrGI T'GTAC_A
Cuts at: 96 2652 96
Size: 2556 3088
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5644
BssSI C'ACGA_G
Cuts at: 3704 4716 3704
Size: 1012 4632
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2418 794
Size: 1624 4020
BstBI TT'CG_AA
Cuts at: 2068 4448 2068
Size: 2380 3264
BstEII G'GTnAC_C
Cuts at: 1184 2836 1184
Size: 1652 3992
BstYI r'GATC_y
Cuts at: 1972 2974 3341 3726 3743 5195 5281 5293 5639
Size: 1002 367 385 17 1452 86 12 346
Cuts at: 5639 1972
Size: 1977
Fragments arranged by size:
1977 1452 1002 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2749 513
Size: 552 939 745 3408
Fragments arranged by size:
3408 939 745 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2141 487
Size: 683 56 915 3990
Fragments arranged by size:
3990 915 683 56
BtsI GCAGTG_nn'
Cuts at: 2384 3160 3954 3974 2384
Size: 776 794 20 4054
Fragments arranged by size:
4054 794 776 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5644
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5609
DraI TTT'AAA
Cuts at: 2948 2948
Size: 5644
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4651 809
Size: 507 3335 1802
Fragments arranged by size:
3335 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2814 3091 3980
Size: 54 1425 50 275 18 984 277 889
Cuts at: 3980 5609 8
Size: 1629 43
Fragments arranged by size:
1629 1425 984 889 277 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3091 1537
Size: 275 1279 4090
Fragments arranged by size:
4090 1279 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2084 2256 2550 3574 1353
Size: 731 172 294 1024 3423
Fragments arranged by size:
3423 1024 731 294 172
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4231 4605 4751 387
Size: 1064 138 2642 374 146 1280
Fragments arranged by size:
2642 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2622 2622
Size: 5644
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2035 2081 2534 3707 4304 5091 5359
Size: 393 403 46 453 1173 597 787 268
Cuts at: 5359 1239
Size: 1524
Fragments arranged by size:
1524 1173 787 597 453 403 393 268
46
EcoO109I rG'GnC_Cy
Cuts at: 2415 2643 2415
Size: 228 5416
EcoRI G'AATT_C
Cuts at: 2953 3331 2953
Size: 378 5266
EcoRV GAT'ATC
Cuts at: 2209 2209
Size: 5644
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2514 2608 2514
Size: 94 5550
FspI TGC'GCA
Cuts at: 4150 4150
Size: 5644
HaeII r_GCGC'y
Cuts at: 1678 4791 1678
Size: 3113 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2140 2172 2767 2799 4285
Size: 32 135 32 568 32 595 32 1486
Cuts at: 4285 4317 4359 4391 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1486 595 568 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2988 3234 669
Size: 988 281 1050 246 3079
Fragments arranged by size:
3079 1050 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2703 748
Size: 1955 3689
HpaI GTT'AAC
Cuts at: 3234 3234
Size: 5644
KpnI G_GTAC'C
Cuts at: 2969 2969
Size: 5644
MfeI C'AATT_G
Cuts at: 3243 3243
Size: 5644
MluI A'CGCG_T
Cuts at: 5520 5520
Size: 5644
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2498 4758 4942 1305
Size: 1193 2260 184 2007
Fragments arranged by size:
2260 2007 1193 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3822
Fragments arranged by size:
3822 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2483 2765 2831 3602 3961 4120 518
Size: 648 1317 282 66 771 359 159 2042
Fragments arranged by size:
2042 1317 771 648 359 282 159 66
MspA1I CmG'CkG
Cuts at: 1606 2819 3060 3737 4885 5130 1606
Size: 1213 241 677 1148 245 2120
Fragments arranged by size:
2120 1213 1148 677 245 241
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4153
Fragments arranged by size:
4153 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5644
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5644
NotI GC'GGCC_GC
Cuts at: 3091 3091
Size: 5644
NspI r_CATG'y
Cuts at: 1132 1229 3002 4547 1132
Size: 97 1773 1545 2229
Fragments arranged by size:
2229 1773 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2689 1348
Size: 1341 4303
PciI A'CATG_T
Cuts at: 4543 4543
Size: 5644
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5644
PfoI T'CCnGG_A
Cuts at: 2058 2058
Size: 5644
PmeI GTTT'AAAC
Cuts at: 2948 2948
Size: 5644
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3713 3745 5226 5258 3713
Size: 32 1481 32 4099
Fragments arranged by size:
4099 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2643 2643
Size: 5644
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5644
PsiI TTA'TAA
Cuts at: 3214 3214
Size: 5644
PstI C_TGCA'G
Cuts at: 830 2996 830
Size: 2166 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5644
SacI G_AGCT'C
Cuts at: 2624 2624
Size: 5644
SalI G'TCGA_C
Cuts at: 1936 2986 1936
Size: 1050 4594
SbfI CC_TGCA'GG
Cuts at: 2996 2996
Size: 5644
ScaI AGT'ACT
Cuts at: 3892 5604 3892
Size: 1712 3932
SfcI C'TryA_G
Cuts at: 826 1045 2503 2863 2992 4127 4808 4999 826
Size: 219 1458 360 129 1135 681 191 1471
Fragments arranged by size:
1471 1458 1135 681 360 219 191 129
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5644
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5644
SmaI CCC'GGG
Cuts at: 1872 2971 1872
Size: 1099 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2869 3748 4649 4911 820
Size: 197 926 926 879 901 262 1553
Fragments arranged by size:
1553 926 926 901 879 262 197
SnaBI TAC'GTA
Cuts at: 493 3435 493
Size: 2942 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5644
SphI G_CATG'C
Cuts at: 1132 3002 1132
Size: 1870 3774
SspI AAT'ATT
Cuts at: 5 52 3568 5
Size: 47 3516 2081
Fragments arranged by size:
3516 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5644
StyI C'CwwG_G
Cuts at: 513 1065 2004 2254 3083 513
Size: 552 939 250 829 3074
Fragments arranged by size:
3074 939 829 552 250
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2434 2702 3671 3688 3841 4026 4471
Size: 1255 854 268 969 17 153 185 445
Cuts at: 4471 325
Size: 1498
Fragments arranged by size:
1498 1255 969 854 445 268 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2175 2652 3890
Size: 274 80 33 51 1443 198 477 1238
Cuts at: 3890 5602 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1238 477 274 198 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2430 2462 5604 5636 2430
Size: 32 3142 32 2438
Fragments arranged by size:
3142 2438 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5644
XbaI T'CTAG_A
Cuts at: 2980 2980
Size: 5644
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5644
XmaI C'CCGG_G
Cuts at: 1870 2969 1870
Size: 1099 4545
XmnI GAAnn'nnTTC
Cuts at: 3773 3773
Size: 5644
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3452 276
Size: 53 83 186 936 1918 2468
Fragments arranged by size:
2468 1918 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI