Restriction map for
pFN21AE5974
(Circular) (Six-base) MAPSORT of: pFN21AE5974.seq Check: 1413 from: 1 to: 5641
pFN21AE5974 5641 bp
With 182 enzymes: SgfI *
December 8, 2014 13:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3451 278
Size: 53 83 186 936 1915 2468
Fragments arranged by size:
2468 1915 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2984 1937
Size: 1047 4594
Acc65I G'GTAC_C
Cuts at: 2962 2962
Size: 5641
AclI AA'CG_TT
Cuts at: 1563 3768 4141 1563
Size: 2205 373 3063
Fragments arranged by size:
3063 2205 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2299 3704 5088 5356 1632
Size: 667 1405 1384 268 1917
Fragments arranged by size:
1917 1405 1384 667 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5444
AflIII A'CryG_T
Cuts at: 1203 2223 2938 4540 5517 1203
Size: 1020 715 1602 977 1327
Fragments arranged by size:
1602 1327 1020 977 715
AgeI A'CCGG_T
Cuts at: 4472 4472
Size: 5641
AhdI GACnn_n'nnGTC
Cuts at: 4370 4370
Size: 5641
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5641
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5641
AlwNI CAG_nnn'CTG
Cuts at: 2080 3384 4956 2080
Size: 1304 1572 2765
Fragments arranged by size:
2765 1572 1304
ApaLI G'TGCA_C
Cuts at: 2329 3698 4854 2329
Size: 1369 1156 3116
Fragments arranged by size:
3116 1369 1156
ApoI r'AATT_y
Cuts at: 1493 2891 2950 3176 3328 5437 1493
Size: 1398 59 226 152 2109 1697
Fragments arranged by size:
2109 1697 1398 226 152 59
AseI AT'TA_AT
Cuts at: 160 1032 4195 160
Size: 872 3163 1606
Fragments arranged by size:
3163 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5641
AvaI C'yCGr_G
Cuts at: 1870 1943 2416 2966 1870
Size: 73 473 550 4545
Fragments arranged by size:
4545 550 473 73
BamHI G'GATC_C
Cuts at: 2971 3338 2971
Size: 367 5274
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2962 4417 618
Size: 325 217 627 1175 1455 1842
Fragments arranged by size:
1842 1455 1175 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2198 2813 2960 1391
Size: 807 615 147 4072
Fragments arranged by size:
4072 807 615 147
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2746 928
Size: 971 847 3823
Fragments arranged by size:
3823 971 847
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2266 2300 3832 3866 1903
Size: 34 329 34 1532 34 3678
Fragments arranged by size:
3678 1532 329 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3533 4743 3533
Size: 1210 4431
BclI T'GATC_A
Cuts at: 1548 2475 1548
Size: 927 4714
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2357 4252 136
Size: 107 122 71 631 605 685 1895 1525
Fragments arranged by size:
1895 1525 685 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2214 5636 2214
Size: 3422 2219
BlpI GC'TnA_GC
Cuts at: 3058 3058
Size: 5641
Bme1580I G_kGCm'C
Cuts at: 1790 2333 3702 4858 1790
Size: 543 1369 1156 2573
Fragments arranged by size:
2573 1369 1156 543
BmgBI CAC'GTC
Cuts at: 1318 2404 1318
Size: 1086 4555
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3372 4330 442
Size: 416 53 83 399 24 1955 958 1753
Fragments arranged by size:
1955 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5641
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2173 2231 2249 2270 2414 2479 2728 4301
Size: 934 58 18 21 144 65 249 1573
Cuts at: 4301 1239
Size: 2579
Fragments arranged by size:
2579 1573 934 249 144 65 58 21
18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2543 3766 4631 4929 2543
Size: 1223 865 298 3255
Fragments arranged by size:
3255 1223 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4304 882
Size: 617 2805 2219
Fragments arranged by size:
2805 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2224 2374 3432 493
Size: 711 1020 150 1058 2702
Fragments arranged by size:
2702 1058 1020 711 150
BsaBI GATnn'nnATC
Cuts at: 5635 5635
Size: 5641
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2901 3448 3830 4465
Size: 53 83 186 936 1368 547 382 635
Cuts at: 4465 275
Size: 1451
Fragments arranged by size:
1451 1368 936 635 547 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4074 4472 4746 4893 5304 4074
Size: 398 274 147 411 4411
Fragments arranged by size:
4411 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2204 2234 1178
Size: 30 165 30 801 30 4585
Fragments arranged by size:
4585 801 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2081 2252 1186
Size: 895 171 4575
Fragments arranged by size:
4575 895 171
BseYI C'CCAG_C
Cuts at: 4844 5340 4844
Size: 496 5145
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2554 1163
Size: 573 818 4250
Fragments arranged by size:
4250 818 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2368 3091 3852 4880 1540
Size: 275 186 367 723 761 1028 2301
Fragments arranged by size:
2301 1028 761 723 367 275 186
BsiHKAI G_wGCw'C
Cuts at: 2198 2333 2813 3702 3787 4858 2198
Size: 135 480 889 85 1071 2981
Fragments arranged by size:
2981 1071 889 480 135 85
BsmI GAATG_Cn'
Cuts at: 3152 3245 3152
Size: 93 5548
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2198 2333 2813 2960 3702 3787 4858
Size: 399 408 135 480 147 742 85 1071
Cuts at: 4858 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 480 408 399 147 135
85
BspEI T'CCGG_A
Cuts at: 5304 5304
Size: 5641
BspHI T'CATG_A
Cuts at: 3530 5260 3530
Size: 1730 3911
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2088 2732 2996 844
Size: 1244 644 264 3489
Fragments arranged by size:
3489 1244 644 264
BsrBI CCG'CTC
Cuts at: 1394 1823 2635 3528 1394
Size: 429 812 893 3507
Fragments arranged by size:
3507 893 812 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4136 4310 65
Size: 1183 2888 174 1396
Fragments arranged by size:
2888 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2699 4285 4472 1700
Size: 110 889 1586 187 2869
Fragments arranged by size:
2869 1586 889 187 110
BsrGI T'GTAC_A
Cuts at: 96 2835 96
Size: 2739 2902
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5641
BssSI C'ACGA_G
Cuts at: 3701 4713 3701
Size: 1012 4629
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2080 794
Size: 1286 4355
BstBI TT'CG_AA
Cuts at: 4445 4445
Size: 5641
BstEII G'GTnAC_C
Cuts at: 1184 2746 1184
Size: 1562 4079
BstXI CCAn_nnnn'nTGG
Cuts at: 2248 2248
Size: 5641
BstYI r'GATC_y
Cuts at: 1972 2214 2971 3338 3723 3740 5192 5278 5290
Size: 242 757 367 385 17 1452 86 12
Cuts at: 5290 5636 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 757 385 367 346 242 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2879 2879
Size: 5641
BtgI C'CryG_G
Cuts at: 513 1065 2058 2355 2742 513
Size: 552 993 297 387 3412
Fragments arranged by size:
3412 993 552 387 297
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4902
Fragments arranged by size:
4902 683 56
BtsI GCAGTG_nn'
Cuts at: 2583 3157 3951 3971 2583
Size: 574 794 20 4253
Fragments arranged by size:
4253 794 574 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5641
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5606
DraI TTT'AAA
Cuts at: 2945 2945
Size: 5641
DraIII CAC_nnn'GTG
Cuts at: 2337 2337
Size: 5641
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4648 809
Size: 507 3332 1802
Fragments arranged by size:
3332 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2133 2376 2640
Size: 54 1425 50 275 18 303 243 264
Cuts at: 2640 2694 3088 3977 5606 8
Size: 54 394 889 1629 43
Fragments arranged by size:
1629 1425 889 394 303 275 264 243
54 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3088 1537
Size: 275 1276 4090
Fragments arranged by size:
4090 1276 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2090 2099 3571 1353
Size: 737 9 1472 3423
Fragments arranged by size:
3423 1472 737 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4228 4602 4748 387
Size: 1064 138 2639 374 146 1280
Fragments arranged by size:
2639 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2196 2811 2196
Size: 615 5026
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2173 2231 2249 2270 2299 2414 2479
Size: 393 541 58 18 21 29 115 65
Cuts at: 2479 2728 3704 4301 5088 5356 1239
Size: 249 976 597 787 268 1524
Fragments arranged by size:
1524 976 787 597 541 393 268 249
115 65 58 29 21 18
EcoRI G'AATT_C
Cuts at: 2950 3328 2950
Size: 378 5263
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2920 2920
Size: 5641
FspI TGC'GCA
Cuts at: 2684 4147 2684
Size: 1463 4178
HaeII r_GCGC'y
Cuts at: 1678 4788 1678
Size: 3110 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4282 4314 4356 4388 1373
Size: 32 135 32 2710 32 42 32 2626
Fragments arranged by size:
2710 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2985 3231 669
Size: 988 281 1047 246 3079
Fragments arranged by size:
3079 1047 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5641
HpaI GTT'AAC
Cuts at: 3231 3231
Size: 5641
KpnI G_GTAC'C
Cuts at: 2966 2966
Size: 5641
MfeI C'AATT_G
Cuts at: 3240 3240
Size: 5641
MluI A'CGCG_T
Cuts at: 5517 5517
Size: 5641
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2541 2838 4755 4939 1305
Size: 1236 297 1917 184 2007
Fragments arranged by size:
2007 1917 1236 297 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2135 2378 2642 10
Size: 54 1425 343 303 243 264 3009
Fragments arranged by size:
3009 1425 343 303 264 243 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2045 2471 2624 3599 3958 4117 518
Size: 648 879 426 153 975 359 159 2042
Fragments arranged by size:
2042 975 879 648 426 359 159 153
MspA1I CmG'CkG
Cuts at: 1606 2392 2434 3057 3734 4882 5127 1606
Size: 786 42 623 677 1148 245 2120
Fragments arranged by size:
2120 1148 786 677 623 245 42
NcoI C'CATG_G
Cuts at: 513 1065 2355 513
Size: 552 1290 3799
Fragments arranged by size:
3799 1290 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5641
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5641
NotI GC'GGCC_GC
Cuts at: 3088 3088
Size: 5641
NspI r_CATG'y
Cuts at: 1132 1229 2999 4544 1132
Size: 97 1770 1545 2229
Fragments arranged by size:
2229 1770 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2066 1348
Size: 718 4923
PciI A'CATG_T
Cuts at: 4540 4540
Size: 5641
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2437 1108
Size: 1329 4312
PfoI T'CCnGG_A
Cuts at: 2208 2208
Size: 5641
PmeI GTTT'AAAC
Cuts at: 2945 2945
Size: 5641
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3710 3742 5223 5255 3710
Size: 32 1481 32 4096
Fragments arranged by size:
4096 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5641
PsiI TTA'TAA
Cuts at: 3211 3211
Size: 5641
PstI C_TGCA'G
Cuts at: 830 2076 2590 2993 830
Size: 1246 514 403 3478
Fragments arranged by size:
3478 1246 514 403
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5641
PvuII CAG'CTG
Cuts at: 2434 2434
Size: 5641
SacI G_AGCT'C
Cuts at: 2198 2813 2198
Size: 615 5026
SalI G'TCGA_C
Cuts at: 1936 2983 1936
Size: 1047 4594
SapI GCTCTTCn'nnn_
Cuts at: 2090 2090
Size: 5641
SbfI CC_TGCA'GG
Cuts at: 2993 2993
Size: 5641
ScaI AGT'ACT
Cuts at: 3889 5601 3889
Size: 1712 3929
SfcI C'TryA_G
Cuts at: 826 1045 2072 2586 2989 4124 4805 4996 826
Size: 219 1027 514 403 1135 681 191 1471
Fragments arranged by size:
1471 1135 1027 681 514 403 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2357 2357
Size: 5641
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5641
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5641
SmaI CCC'GGG
Cuts at: 1872 2418 2968 1872
Size: 546 550 4545
Fragments arranged by size:
4545 550 546
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2558 3745 4646 4908 820
Size: 197 926 615 1187 901 262 1553
Fragments arranged by size:
1553 1187 926 901 615 262 197
SnaBI TAC'GTA
Cuts at: 493 3432 493
Size: 2939 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5641
SphI G_CATG'C
Cuts at: 1132 2999 1132
Size: 1867 3774
SspI AAT'ATT
Cuts at: 5 52 3565 5
Size: 47 3513 2081
Fragments arranged by size:
3513 2081 47
StuI AGG'CCT
Cuts at: 1517 2915 1517
Size: 1398 4243
StyI C'CwwG_G
Cuts at: 513 1065 2025 2355 2916 3080 513
Size: 552 960 330 561 164 3074
Fragments arranged by size:
3074 960 561 552 330 164
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3668 3685 3838 4023 4468 325
Size: 1255 2088 17 153 185 445 1498
Fragments arranged by size:
2088 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 2835 3887
Size: 274 80 33 51 1443 204 654 1052
Cuts at: 3887 5599 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1052 654 274 204 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5601 5633 5601
Size: 32 5609
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5641
XbaI T'CTAG_A
Cuts at: 2977 2977
Size: 5641
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2206 2560 2590 2206
Size: 354 30 5257
Fragments arranged by size:
5257 354 30
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5641
XmaI C'CCGG_G
Cuts at: 1870 2416 2966 1870
Size: 546 550 4545
Fragments arranged by size:
4545 550 546
XmnI GAAnn'nnTTC
Cuts at: 2531 3770 2531
Size: 1239 4402
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3449 276
Size: 53 83 186 936 1915 2468
Fragments arranged by size:
2468 1915 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I EcoNI
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacII SanDI SexAI SfoI SrfI SwaI