Restriction map for
pFN21AE5964
(Circular) (Six-base) MAPSORT of: pFN21AE5964.seq Check: 4117 from: 1 to: 5641
pFN21AE5964 5641 bp
With 182 enzymes: SgfI *
December 8, 2014 13:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3451 278
Size: 53 83 186 936 1915 2468
Fragments arranged by size:
2468 1915 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2847 2984 1937
Size: 910 137 4594
Fragments arranged by size:
4594 910 137
Acc65I G'GTAC_C
Cuts at: 2962 2962
Size: 5641
AclI AA'CG_TT
Cuts at: 1563 3768 4141 1563
Size: 2205 373 3063
Fragments arranged by size:
3063 2205 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2185 2707 2724 3704 5088 5356 1632
Size: 553 522 17 980 1384 268 1917
Fragments arranged by size:
1917 1384 980 553 522 268 17
AflII C'TTAA_G
Cuts at: 820 1017 2498 820
Size: 197 1481 3963
Fragments arranged by size:
3963 1481 197
AflIII A'CryG_T
Cuts at: 1203 2459 4540 5517 1203
Size: 1256 2081 977 1327
Fragments arranged by size:
2081 1327 1256 977
AgeI A'CCGG_T
Cuts at: 4472 4472
Size: 5641
AhdI GACnn_n'nnGTC
Cuts at: 4370 4370
Size: 5641
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5641
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2933 1184
Size: 1749 3892
AlwNI CAG_nnn'CTG
Cuts at: 2216 2730 3384 4956 2216
Size: 514 654 1572 2901
Fragments arranged by size:
2901 1572 654 514
ApaLI G'TGCA_C
Cuts at: 3698 4854 3698
Size: 1156 4485
ApoI r'AATT_y
Cuts at: 1493 2033 2950 3176 3328 5437 1493
Size: 540 917 226 152 2109 1697
Fragments arranged by size:
2109 1697 917 540 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2609 4195 160
Size: 872 1577 1586 1606
Fragments arranged by size:
1606 1586 1577 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5641
AvaI C'yCGr_G
Cuts at: 1870 1943 2966 1870
Size: 73 1023 4545
Fragments arranged by size:
4545 1023 73
BamHI G'GATC_C
Cuts at: 2971 3338 2971
Size: 367 5274
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2962 4417 618
Size: 325 217 627 1175 1455 1842
Fragments arranged by size:
1842 1455 1175 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2960 1391
Size: 1569 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2438 2713 928
Size: 971 539 275 3856
Fragments arranged by size:
3856 971 539 275
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3832 3866 1903
Size: 34 1895 34 3678
Fragments arranged by size:
3678 1895 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2270 3533 4743 2270
Size: 1263 1210 3168
Fragments arranged by size:
3168 1263 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5641
BfrBI ATG'CAT
Cuts at: 2649 2649
Size: 5641
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4252 136
Size: 107 122 71 631 605 2580 1525
Fragments arranged by size:
2580 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5636 5636
Size: 5641
BlpI GC'TnA_GC
Cuts at: 2777 3058 2777
Size: 281 5360
Bme1580I G_kGCm'C
Cuts at: 1790 3702 4858 1790
Size: 1912 1156 2573
Fragments arranged by size:
2573 1912 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5641
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3372 4330 442
Size: 416 53 83 399 24 1955 958 1753
Fragments arranged by size:
1955 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5641
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2367 4301 1239
Size: 1128 1934 2579
Fragments arranged by size:
2579 1934 1128
Bpu10I CC'TnA_GC
Cuts at: 2015 2015
Size: 5641
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3766 4631 4929 3766
Size: 865 298 4478
Fragments arranged by size:
4478 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4304 882
Size: 617 2805 2219
Fragments arranged by size:
2805 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2279 2462 3432 493
Size: 711 1075 183 970 2702
Fragments arranged by size:
2702 1075 970 711 183
BsaBI GATnn'nnATC
Cuts at: 5635 5635
Size: 5641
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3448 3830 4465 275
Size: 53 83 186 936 1915 382 635 1451
Fragments arranged by size:
1915 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4074 4472 4746 4893 5304 4074
Size: 398 274 147 411 4411
Fragments arranged by size:
4411 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2340 2370 2478 2508 1178
Size: 30 165 30 937 30 108 30 4311
Fragments arranged by size:
4311 937 165 108 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5641
BseYI C'CCAG_C
Cuts at: 2163 4844 5340 2163
Size: 2681 496 2464
Fragments arranged by size:
2681 2464 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5068
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3091 3852 4880 1540
Size: 275 186 1090 761 1028 2301
Fragments arranged by size:
2301 1090 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2871 3702 3787 4858 2871
Size: 831 85 1071 3654
Fragments arranged by size:
3654 1071 831 85
BsmI GAATG_Cn'
Cuts at: 2058 2530 3152 3245 2058
Size: 472 622 93 4454
Fragments arranged by size:
4454 622 472 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2871 2960 3702 3787 4858 1391
Size: 399 1081 89 742 85 1071 2174
Fragments arranged by size:
2174 1081 1071 742 399 89 85
BspEI T'CCGG_A
Cuts at: 5304 5304
Size: 5641
BspHI T'CATG_A
Cuts at: 3530 5260 3530
Size: 1730 3911
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2859 2996 844
Size: 2015 137 3489
Fragments arranged by size:
3489 2015 137
BsrBI CCG'CTC
Cuts at: 1394 1823 3528 1394
Size: 429 1705 3507
Fragments arranged by size:
3507 1705 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2118 4136 4310 65
Size: 1183 870 2018 174 1396
Fragments arranged by size:
2018 1396 1183 870 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2054 4285 4472 1700
Size: 110 244 2231 187 2869
Fragments arranged by size:
2869 2231 244 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5641
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5641
BssSI C'ACGA_G
Cuts at: 3701 4713 3701
Size: 1012 4629
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5641
BstBI TT'CG_AA
Cuts at: 4445 4445
Size: 5641
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5641
BstXI CCAn_nnnn'nTGG
Cuts at: 2221 2221
Size: 5641
BstYI r'GATC_y
Cuts at: 1972 2088 2971 3338 3723 3740 5192 5278 5290
Size: 116 883 367 385 17 1452 86 12
Cuts at: 5290 5636 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 883 385 367 346 116 86
17 12
BstZ17I GTA'TAC
Cuts at: 2848 2848
Size: 5641
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4150
Fragments arranged by size:
4150 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4902
Fragments arranged by size:
4902 683 56
BtsI GCAGTG_nn'
Cuts at: 3157 3951 3971 3157
Size: 794 20 4827
Fragments arranged by size:
4827 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5641
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2472 2507 529
Size: 35 1908 35 3663
Fragments arranged by size:
3663 1908 35 35
DraI TTT'AAA
Cuts at: 2945 2945
Size: 5641
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4648 809
Size: 507 3332 1802
Fragments arranged by size:
3332 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2382 3088 3977
Size: 54 1425 50 275 18 552 706 889
Cuts at: 3977 5606 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 706 552 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3088 1537
Size: 275 1276 4090
Fragments arranged by size:
4090 1276 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2204 3571 1353
Size: 851 1367 3423
Fragments arranged by size:
3423 1367 851
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4228 4602 4748 387
Size: 1064 138 2639 374 146 1280
Fragments arranged by size:
2639 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2185 2367 2707 2724 3704 4301 5088
Size: 393 553 182 340 17 980 597 787
Cuts at: 5088 5356 1239
Size: 268 1524
Fragments arranged by size:
1524 980 787 597 553 393 340 268
182 17
EcoRI G'AATT_C
Cuts at: 2950 3328 2950
Size: 378 5263
FspI TGC'GCA
Cuts at: 4147 4147
Size: 5641
HaeII r_GCGC'y
Cuts at: 1678 4788 1678
Size: 3110 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4282 4314 4356 4388 1373
Size: 32 135 32 2710 32 42 32 2626
Fragments arranged by size:
2710 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2052 2247 2985 3231 669
Size: 988 281 114 195 738 246 3079
Fragments arranged by size:
3079 988 738 281 246 195 114
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5641
HpaI GTT'AAC
Cuts at: 3231 3231
Size: 5641
KpnI G_GTAC'C
Cuts at: 2966 2966
Size: 5641
MfeI C'AATT_G
Cuts at: 3240 3240
Size: 5641
MluI A'CGCG_T
Cuts at: 5517 5517
Size: 5641
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4755 4939 1305
Size: 3450 184 2007
Fragments arranged by size:
3450 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2384 10
Size: 54 1425 343 552 3267
Fragments arranged by size:
3267 1425 552 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2336 2438 2556 2771 3599 3958 4117
Size: 648 1170 102 118 215 828 359 159
Cuts at: 4117 518
Size: 2042
Fragments arranged by size:
2042 1170 828 648 359 215 159 118
102
MspA1I CmG'CkG
Cuts at: 1606 3057 3734 4882 5127 1606
Size: 1451 677 1148 245 2120
Fragments arranged by size:
2120 1451 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4150
Fragments arranged by size:
4150 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5641
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5641
NotI GC'GGCC_GC
Cuts at: 3088 3088
Size: 5641
NsiI A_TGCA'T
Cuts at: 2651 2651
Size: 5641
NspI r_CATG'y
Cuts at: 1132 1229 2577 2776 2999 4544 1132
Size: 97 1348 199 223 1545 2229
Fragments arranged by size:
2229 1545 1348 223 199 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5641
PciI A'CATG_T
Cuts at: 4540 4540
Size: 5641
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5641
PmeI GTTT'AAAC
Cuts at: 2945 2945
Size: 5641
PmlI CAC'GTG
Cuts at: 2279 2462 2279
Size: 183 5458
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3710 3742 5223 5255 3710
Size: 32 1481 32 4096
Fragments arranged by size:
4096 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5641
PsiI TTA'TAA
Cuts at: 3211 3211
Size: 5641
PstI C_TGCA'G
Cuts at: 830 2993 830
Size: 2163 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5641
SalI G'TCGA_C
Cuts at: 1936 2983 1936
Size: 1047 4594
SbfI CC_TGCA'GG
Cuts at: 2993 2993
Size: 5641
ScaI AGT'ACT
Cuts at: 2472 3889 5601 2472
Size: 1417 1712 2512
Fragments arranged by size:
2512 1712 1417
SfcI C'TryA_G
Cuts at: 826 1045 2361 2466 2515 2989 4124 4805 4996
Size: 219 1316 105 49 474 1135 681 191
Cuts at: 4996 826
Size: 1471
Fragments arranged by size:
1471 1316 1135 681 474 219 191 105
49
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5641
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5641
SmaI CCC'GGG
Cuts at: 1872 2968 1872
Size: 1096 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2498 3745 4646 4908 820
Size: 197 926 555 1247 901 262 1553
Fragments arranged by size:
1553 1247 926 901 555 262 197
SnaBI TAC'GTA
Cuts at: 493 3432 493
Size: 2939 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5641
SphI G_CATG'C
Cuts at: 1132 2999 1132
Size: 1867 3774
SspI AAT'ATT
Cuts at: 5 52 2478 3565 5
Size: 47 2426 1087 2081
Fragments arranged by size:
2426 2081 1087 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5641
StyI C'CwwG_G
Cuts at: 513 1065 2004 2787 3080 513
Size: 552 939 783 293 3074
Fragments arranged by size:
3074 939 783 552 293
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2796 3668 3685 3838 4023 4468 325
Size: 1255 1216 872 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1216 872 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2470 3887 5599
Size: 274 80 33 51 1443 493 1417 1712
Cuts at: 5599 96
Size: 138
Fragments arranged by size:
1712 1443 1417 493 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2340 2372 5601 5633 2340
Size: 32 3229 32 2348
Fragments arranged by size:
3229 2348 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5641
XbaI T'CTAG_A
Cuts at: 2977 2977
Size: 5641
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5641
XmaI C'CCGG_G
Cuts at: 1870 2966 1870
Size: 1096 4545
XmnI GAAnn'nnTTC
Cuts at: 3770 3770
Size: 5641
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3449 276
Size: 53 83 186 936 1915 2468
Fragments arranged by size:
2468 1915 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI
PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI BsiWI BsmBI Bsu36I DraIII EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI