Restriction map for
pFN21AE5963
(Circular) (Six-base) MAPSORT of: pFN21AE5963.seq Check: 4086 from: 1 to: 5641
pFN21AE5963 5641 bp
With 182 enzymes: SgfI *
December 8, 2014 13:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3451 278
Size: 53 83 186 936 1915 2468
Fragments arranged by size:
2468 1915 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2984 1937
Size: 1047 4594
Acc65I G'GTAC_C
Cuts at: 2962 2962
Size: 5641
AclI AA'CG_TT
Cuts at: 1563 3768 4141 1563
Size: 2205 373 3063
Fragments arranged by size:
3063 2205 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3704 5088 5356 1632
Size: 2072 1384 268 1917
Fragments arranged by size:
2072 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5444
AflIII A'CryG_T
Cuts at: 1203 2103 4540 5517 1203
Size: 900 2437 977 1327
Fragments arranged by size:
2437 1327 977 900
AgeI A'CCGG_T
Cuts at: 4472 4472
Size: 5641
AhdI GACnn_n'nnGTC
Cuts at: 4370 4370
Size: 5641
AleI CACnn'nnGTG
Cuts at: 1166 2252 2414 1166
Size: 1086 162 4393
Fragments arranged by size:
4393 1086 162
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5641
AlwNI CAG_nnn'CTG
Cuts at: 2760 3384 4956 2760
Size: 624 1572 3445
Fragments arranged by size:
3445 1572 624
ApaLI G'TGCA_C
Cuts at: 3698 4854 3698
Size: 1156 4485
ApoI r'AATT_y
Cuts at: 1493 2011 2208 2330 2679 2950 3176 3328 5437
Size: 518 197 122 349 271 226 152 2109
Cuts at: 5437 1493
Size: 1697
Fragments arranged by size:
2109 1697 518 349 271 226 197 152
122
AseI AT'TA_AT
Cuts at: 160 1032 4195 160
Size: 872 3163 1606
Fragments arranged by size:
3163 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5641
AvaI C'yCGr_G
Cuts at: 1870 1943 2966 1870
Size: 73 1023 4545
Fragments arranged by size:
4545 1023 73
BamHI G'GATC_C
Cuts at: 2971 3338 2971
Size: 367 5274
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2962 4417 618
Size: 325 217 627 1175 1455 1842
Fragments arranged by size:
1842 1455 1175 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2960 1391
Size: 1569 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4670
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3832 3866 1903
Size: 34 1895 34 3678
Fragments arranged by size:
3678 1895 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3533 4743 3533
Size: 1210 4431
BclI T'GATC_A
Cuts at: 1548 2127 2460 2521 1548
Size: 579 333 61 4668
Fragments arranged by size:
4668 579 333 61
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4252 136
Size: 107 122 71 631 605 2580 1525
Fragments arranged by size:
2580 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5636 5636
Size: 5641
BlpI GC'TnA_GC
Cuts at: 2295 3058 2295
Size: 763 4878
Bme1580I G_kGCm'C
Cuts at: 1790 3702 4858 1790
Size: 1912 1156 2573
Fragments arranged by size:
2573 1912 1156
BmgBI CAC'GTC
Cuts at: 1318 2220 1318
Size: 902 4739
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2127 3372 4330
Size: 416 53 83 399 24 710 1245 958
Cuts at: 4330 442
Size: 1753
Fragments arranged by size:
1753 1245 958 710 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5641
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4301 1239
Size: 3062 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3766 4631 4929 3766
Size: 865 298 4478
Fragments arranged by size:
4478 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4304 882
Size: 617 2805 2219
Fragments arranged by size:
2805 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3432 493
Size: 711 2228 2702
Fragments arranged by size:
2702 2228 711
BsaBI GATnn'nnATC
Cuts at: 2132 2157 5635 2132
Size: 25 3478 2138
Fragments arranged by size:
3478 2138 25
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3448 3830 4465 275
Size: 53 83 186 936 1915 382 635 1451
Fragments arranged by size:
1915 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2664 4074 4472 4746 4893 5304 2664
Size: 1410 398 274 147 411 3001
Fragments arranged by size:
3001 1410 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2808 2838 1178
Size: 30 165 30 1405 30 3981
Fragments arranged by size:
3981 1405 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2713 2835 1186
Size: 1527 122 3992
Fragments arranged by size:
3992 1527 122
BseYI C'CCAG_C
Cuts at: 4844 5340 4844
Size: 496 5145
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5068
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3091 3852 4880 1540
Size: 275 186 1090 761 1028 2301
Fragments arranged by size:
2301 1090 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2411 2829 3702 3787 4858 2411
Size: 418 873 85 1071 3194
Fragments arranged by size:
3194 1071 873 418 85
BsmI GAATG_Cn'
Cuts at: 2229 3152 3245 2229
Size: 923 93 4625
Fragments arranged by size:
4625 923 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2411 2829 2960 3702 3787 4858 1391
Size: 399 621 418 131 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 621 418 399 131 85
BspEI T'CCGG_A
Cuts at: 2664 5304 2664
Size: 2640 3001
BspHI T'CATG_A
Cuts at: 2403 3530 5260 2403
Size: 1127 1730 2784
Fragments arranged by size:
2784 1730 1127
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2059 2996 844
Size: 1215 937 3489
Fragments arranged by size:
3489 1215 937
BsrBI CCG'CTC
Cuts at: 1394 1823 3528 1394
Size: 429 1705 3507
Fragments arranged by size:
3507 1705 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4136 4310 65
Size: 1183 2888 174 1396
Fragments arranged by size:
2888 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4285 4472 1700
Size: 110 2475 187 2869
Fragments arranged by size:
2869 2475 187 110
BsrGI T'GTAC_A
Cuts at: 96 2772 96
Size: 2676 2965
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5641
BssSI C'ACGA_G
Cuts at: 3701 4713 3701
Size: 1012 4629
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5641
BstBI TT'CG_AA
Cuts at: 4445 4445
Size: 5641
BstEII G'GTnAC_C
Cuts at: 1184 2623 1184
Size: 1439 4202
BstYI r'GATC_y
Cuts at: 1972 2152 2971 3338 3723 3740 5192 5278 5290
Size: 180 819 367 385 17 1452 86 12
Cuts at: 5290 5636 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 819 385 367 346 180 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5089
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4902
Fragments arranged by size:
4902 683 56
BtsI GCAGTG_nn'
Cuts at: 3157 3951 3971 3157
Size: 794 20 4827
Fragments arranged by size:
4827 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5641
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5606
DraI TTT'AAA
Cuts at: 2934 2945 2934
Size: 11 5630
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4648 809
Size: 507 3332 1802
Fragments arranged by size:
3332 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2370 3088 3977
Size: 54 1425 50 275 18 540 718 889
Cuts at: 3977 5606 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 718 540 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3088 1537
Size: 275 1276 4090
Fragments arranged by size:
4090 1276 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2391 2545 3571 1353
Size: 1038 154 1026 3423
Fragments arranged by size:
3423 1038 1026 154
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4228 4602 4748 387
Size: 1064 138 2639 374 146 1280
Fragments arranged by size:
2639 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3704 4301 5088 5356 1239
Size: 393 2072 597 787 268 1524
Fragments arranged by size:
2072 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2950 3328 2950
Size: 378 5263
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2820 2914 2820
Size: 94 5547
FspI TGC'GCA
Cuts at: 4147 4147
Size: 5641
HaeII r_GCGC'y
Cuts at: 1678 4788 1678
Size: 3110 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2229 2261 4282 4314 4356
Size: 32 135 32 657 32 2021 32 42
Cuts at: 4356 4388 1373
Size: 32 2626
Fragments arranged by size:
2626 2021 657 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2985 3231 669
Size: 988 281 1047 246 3079
Fragments arranged by size:
3079 1047 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5641
HpaI GTT'AAC
Cuts at: 3231 3231
Size: 5641
KpnI G_GTAC'C
Cuts at: 2966 2966
Size: 5641
MfeI C'AATT_G
Cuts at: 3240 3240
Size: 5641
MluI A'CGCG_T
Cuts at: 5517 5517
Size: 5641
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2475 4755 4939 1305
Size: 1170 2280 184 2007
Fragments arranged by size:
2280 2007 1170 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2372 10
Size: 54 1425 343 540 3279
Fragments arranged by size:
3279 1425 540 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2252 2414 3599 3958 4117 518
Size: 648 1086 162 1185 359 159 2042
Fragments arranged by size:
2042 1185 1086 648 359 162 159
MspA1I CmG'CkG
Cuts at: 1606 2364 3057 3734 4882 5127 1606
Size: 758 693 677 1148 245 2120
Fragments arranged by size:
2120 1148 758 693 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5089
NdeI CA'TA_TG
Cuts at: 387 2730 387
Size: 2343 3298
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5641
NotI GC'GGCC_GC
Cuts at: 3088 3088
Size: 5641
NspI r_CATG'y
Cuts at: 1132 1229 2107 2999 4544 1132
Size: 97 878 892 1545 2229
Fragments arranged by size:
2229 1545 892 878 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5641
PciI A'CATG_T
Cuts at: 2103 4540 2103
Size: 2437 3204
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5641
PfoI T'CCnGG_A
Cuts at: 2202 2202
Size: 5641
PmeI GTTT'AAAC
Cuts at: 2945 2945
Size: 5641
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3710 3742 5223 5255 3710
Size: 32 1481 32 4096
Fragments arranged by size:
4096 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5641
PsiI TTA'TAA
Cuts at: 2280 3211 2280
Size: 931 4710
PstI C_TGCA'G
Cuts at: 830 2166 2493 2556 2993 830
Size: 1336 327 63 437 3478
Fragments arranged by size:
3478 1336 437 327 63
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5641
SalI G'TCGA_C
Cuts at: 1936 2983 1936
Size: 1047 4594
SbfI CC_TGCA'GG
Cuts at: 2993 2993
Size: 5641
ScaI AGT'ACT
Cuts at: 3889 5601 3889
Size: 1712 3929
SfcI C'TryA_G
Cuts at: 826 1045 2162 2489 2552 2989 4124 4805 4996
Size: 219 1117 327 63 437 1135 681 191
Cuts at: 4996 826
Size: 1471
Fragments arranged by size:
1471 1135 1117 681 437 327 219 191
63
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5641
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5641
SmaI CCC'GGG
Cuts at: 1872 2968 1872
Size: 1096 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3745 4646 4908 820
Size: 197 926 1802 901 262 1553
Fragments arranged by size:
1802 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3432 493
Size: 2939 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5641
SphI G_CATG'C
Cuts at: 1132 2999 1132
Size: 1867 3774
SspI AAT'ATT
Cuts at: 5 52 3565 5
Size: 47 3513 2081
Fragments arranged by size:
3513 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5641
StyI C'CwwG_G
Cuts at: 513 1065 3080 513
Size: 552 2015 3074
Fragments arranged by size:
3074 2015 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2274 3668 3685 3838 4023 4468 325
Size: 1255 694 1394 17 153 185 445 1498
Fragments arranged by size:
1498 1394 1255 694 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2074 2772 3887
Size: 274 80 33 51 1443 97 698 1115
Cuts at: 3887 5599 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1115 698 274 138 97 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2808 2840 5601 5633 2808
Size: 32 2761 32 2816
Fragments arranged by size:
2816 2761 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5641
XbaI T'CTAG_A
Cuts at: 2581 2977 2581
Size: 396 5245
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5641
XmaI C'CCGG_G
Cuts at: 1870 2966 1870
Size: 1096 4545
XmnI GAAnn'nnTTC
Cuts at: 2272 2858 3770 2272
Size: 586 912 4143
Fragments arranged by size:
4143 912 586
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3449 276
Size: 53 83 186 936 1915 2468
Fragments arranged by size:
2468 1915 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI