Restriction map for
pFN21AE5962
(Circular) (Six-base) MAPSORT of: pFN21AE5962.seq Check: 2979 from: 1 to: 5641
pFN21AE5962 5641 bp
With 182 enzymes: SgfI *
December 8, 2014 13:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3451 278
Size: 53 83 186 936 1915 2468
Fragments arranged by size:
2468 1915 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2984 1937
Size: 1047 4594
Acc65I G'GTAC_C
Cuts at: 2962 2962
Size: 5641
AclI AA'CG_TT
Cuts at: 1563 3768 4141 1563
Size: 2205 373 3063
Fragments arranged by size:
3063 2205 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2191 2439 3704 5088 5356 1632
Size: 559 248 1265 1384 268 1917
Fragments arranged by size:
1917 1384 1265 559 268 248
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5444
AflIII A'CryG_T
Cuts at: 1203 2723 2821 4540 5517 1203
Size: 1520 98 1719 977 1327
Fragments arranged by size:
1719 1520 1327 977 98
AgeI A'CCGG_T
Cuts at: 4472 4472
Size: 5641
AhdI GACnn_n'nnGTC
Cuts at: 4370 4370
Size: 5641
AleI CACnn'nnGTG
Cuts at: 1166 2726 1166
Size: 1560 4081
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5641
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2592 2624 2592
Size: 32 5609
AlwNI CAG_nnn'CTG
Cuts at: 3384 4956 3384
Size: 1572 4069
ApaI G_GGCC'C
Cuts at: 2094 2094
Size: 5641
ApaLI G'TGCA_C
Cuts at: 2786 3698 4854 2786
Size: 912 1156 3573
Fragments arranged by size:
3573 1156 912
ApoI r'AATT_y
Cuts at: 1493 2950 3176 3328 5437 1493
Size: 1457 226 152 2109 1697
Fragments arranged by size:
2109 1697 1457 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4195 160
Size: 872 3163 1606
Fragments arranged by size:
3163 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5641
AvaI C'yCGr_G
Cuts at: 1870 1943 2966 1870
Size: 73 1023 4545
Fragments arranged by size:
4545 1023 73
BamHI G'GATC_C
Cuts at: 2971 3338 2971
Size: 367 5274
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2962 4417 618
Size: 325 217 627 1175 1455 1842
Fragments arranged by size:
1842 1455 1175 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2072 2094 2163 2178 2960 1391
Size: 681 22 69 15 782 4072
Fragments arranged by size:
4072 782 681 69 22 15
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4670
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2241 2275 3832 3866 1903
Size: 34 304 34 1557 34 3678
Fragments arranged by size:
3678 1557 304 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2063 2626 3533 4743 2063
Size: 563 907 1210 2961
Fragments arranged by size:
2961 1210 907 563
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5641
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4252 136
Size: 107 122 71 631 605 2580 1525
Fragments arranged by size:
2580 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5636 5636
Size: 5641
BlpI GC'TnA_GC
Cuts at: 3058 3058
Size: 5641
Bme1580I G_kGCm'C
Cuts at: 1790 2094 2790 3702 4858 1790
Size: 304 696 912 1156 2573
Fragments arranged by size:
2573 1156 912 696 304
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5641
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3372 4330 442
Size: 416 53 83 399 24 1955 958 1753
Fragments arranged by size:
1955 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5641
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2084 4301 1239
Size: 845 2217 2579
Fragments arranged by size:
2579 2217 845
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3766 4631 4929 3766
Size: 865 298 4478
Fragments arranged by size:
4478 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2782 4304 882
Size: 617 1283 1522 2219
Fragments arranged by size:
2219 1522 1283 617
BsaAI yAC'GTr
Cuts at: 493 1204 3432 493
Size: 711 2228 2702
Fragments arranged by size:
2702 2228 711
BsaBI GATnn'nnATC
Cuts at: 5635 5635
Size: 5641
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3448 3830 4465 275
Size: 53 83 186 936 1915 382 635 1451
Fragments arranged by size:
1915 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4074 4472 4746 4893 5304 4074
Size: 398 274 147 411 4411
Fragments arranged by size:
4411 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2309 2339 1178
Size: 30 165 30 906 30 4480
Fragments arranged by size:
4480 906 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5641
BseYI C'CCAG_C
Cuts at: 2162 4844 5340 2162
Size: 2682 496 2463
Fragments arranged by size:
2682 2463 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2466 2528 1163
Size: 573 730 62 4276
Fragments arranged by size:
4276 730 573 62
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3091 3852 4880 1540
Size: 275 186 1090 761 1028 2301
Fragments arranged by size:
2301 1090 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2072 2412 2664 2790 2865 3702 3787 4858 2072
Size: 340 252 126 75 837 85 1071 2855
Fragments arranged by size:
2855 1071 837 340 252 126 85 75
BsmI GAATG_Cn'
Cuts at: 2033 3152 3245 2033
Size: 1119 93 4429
Fragments arranged by size:
4429 1119 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2072 2094 2163 2178 2412 2664 2790
Size: 399 282 22 69 15 234 252 126
Cuts at: 2790 2865 2960 3702 3787 4858 1391
Size: 75 95 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 282 252 234 126
95 85 75 69 22 15
BspEI T'CCGG_A
Cuts at: 5304 5304
Size: 5641
BspHI T'CATG_A
Cuts at: 3530 5260 3530
Size: 1730 3911
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2996 844
Size: 2152 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3528 1394
Size: 429 1705 3507
Fragments arranged by size:
3507 1705 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2424 2439 4136 4310 65
Size: 1183 1176 15 1697 174 1396
Fragments arranged by size:
1697 1396 1183 1176 174 15
BsrFI r'CCGG_y
Cuts at: 1700 1810 4285 4472 1700
Size: 110 2475 187 2869
Fragments arranged by size:
2869 2475 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5641
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5641
BssSI C'ACGA_G
Cuts at: 3701 4713 3701
Size: 1012 4629
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2149 794
Size: 1355 4286
BstBI TT'CG_AA
Cuts at: 4445 4445
Size: 5641
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5641
BstXI CCAn_nnnn'nTGG
Cuts at: 2227 2227
Size: 5641
BstYI r'GATC_y
Cuts at: 1972 2513 2971 3338 3723 3740 5192 5278 5290
Size: 541 458 367 385 17 1452 86 12
Cuts at: 5290 5636 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 541 458 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2226 513
Size: 552 939 222 3928
Fragments arranged by size:
3928 939 552 222
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4902
Fragments arranged by size:
4902 683 56
BtsI GCAGTG_nn'
Cuts at: 2234 2685 3157 3951 3971 2234
Size: 451 472 794 20 3904
Fragments arranged by size:
3904 794 472 451 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5641
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5606
DraI TTT'AAA
Cuts at: 2945 2945
Size: 5641
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4648 809
Size: 507 3332 1802
Fragments arranged by size:
3332 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2217 2229 2352
Size: 54 1425 50 275 18 387 12 123
Cuts at: 2352 2376 2682 3088 3977 5606 8
Size: 24 306 406 889 1629 43
Fragments arranged by size:
1629 1425 889 406 387 306 275 123
54 50 43 24 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 3088 1537
Size: 275 1276 4090
Fragments arranged by size:
4090 1276 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2484 3571 1353
Size: 1131 1087 3423
Fragments arranged by size:
3423 1131 1087
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2891 4228 4602 4748 387
Size: 1064 138 1302 1337 374 146 1280
Fragments arranged by size:
1337 1302 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2070 2070
Size: 5641
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2084 2191 2439 3704 4301 5088 5356
Size: 393 452 107 248 1265 597 787 268
Cuts at: 5356 1239
Size: 1524
Fragments arranged by size:
1524 1265 787 597 452 393 268 248
107
EcoO109I rG'GnC_Cy
Cuts at: 2090 2091 2090
Size: 1 5640
EcoRI G'AATT_C
Cuts at: 2950 3328 2950
Size: 378 5263
EcoRV GAT'ATC
Cuts at: 2578 2578
Size: 5641
FspI TGC'GCA
Cuts at: 4147 4147
Size: 5641
HaeII r_GCGC'y
Cuts at: 1678 2886 4788 1678
Size: 1208 1902 2531
Fragments arranged by size:
2531 1902 1208
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4282 4314 4356 4388 1373
Size: 32 135 32 2710 32 42 32 2626
Fragments arranged by size:
2710 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2985 3231 669
Size: 988 281 1047 246 3079
Fragments arranged by size:
3079 1047 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2458 748
Size: 1710 3931
HpaI GTT'AAC
Cuts at: 3231 3231
Size: 5641
KpnI G_GTAC'C
Cuts at: 2966 2966
Size: 5641
MfeI C'AATT_G
Cuts at: 3240 3240
Size: 5641
MluI A'CGCG_T
Cuts at: 5517 5517
Size: 5641
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4755 4939 1305
Size: 3450 184 2007
Fragments arranged by size:
3450 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2219 2231 2354 2378 2684
Size: 54 1425 343 387 12 123 24 306
Cuts at: 2684 10
Size: 2967
Fragments arranged by size:
2967 1425 387 343 306 123 54 24
12
MslI CAynn'nnrTG
Cuts at: 518 1166 2225 2726 3599 3958 4117 518
Size: 648 1059 501 873 359 159 2042
Fragments arranged by size:
2042 1059 873 648 501 359 159
MspA1I CmG'CkG
Cuts at: 1606 2450 3057 3734 4882 5127 1606
Size: 844 607 677 1148 245 2120
Fragments arranged by size:
2120 1148 844 677 607 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2226 513
Size: 552 939 222 3928
Fragments arranged by size:
3928 939 552 222
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5641
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5641
NotI GC'GGCC_GC
Cuts at: 3088 3088
Size: 5641
NspI r_CATG'y
Cuts at: 1132 1229 2727 2807 2825 2923 2999 4544 1132
Size: 97 1498 80 18 98 76 1545 2229
Fragments arranged by size:
2229 1545 1498 98 97 80 76 18
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5641
PciI A'CATG_T
Cuts at: 2723 2821 4540 2723
Size: 98 1719 3824
Fragments arranged by size:
3824 1719 98
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2771 1108
Size: 1663 3978
PmeI GTTT'AAAC
Cuts at: 2945 2945
Size: 5641
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3710 3742 5223 5255 3710
Size: 32 1481 32 4096
Fragments arranged by size:
4096 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5641
PsiI TTA'TAA
Cuts at: 3211 3211
Size: 5641
PspOMI G'GGCC_C
Cuts at: 2090 2090
Size: 5641
PstI C_TGCA'G
Cuts at: 830 2145 2513 2993 830
Size: 1315 368 480 3478
Fragments arranged by size:
3478 1315 480 368
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5641
PvuII CAG'CTG
Cuts at: 2450 2450
Size: 5641
SacI G_AGCT'C
Cuts at: 2072 2072
Size: 5641
SalI G'TCGA_C
Cuts at: 1936 2983 1936
Size: 1047 4594
SbfI CC_TGCA'GG
Cuts at: 2145 2993 2145
Size: 848 4793
ScaI AGT'ACT
Cuts at: 3889 5601 3889
Size: 1712 3929
SfcI C'TryA_G
Cuts at: 826 1045 2141 2509 2989 4124 4805 4996 826
Size: 219 1096 368 480 1135 681 191 1471
Fragments arranged by size:
1471 1135 1096 681 480 368 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5641
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5641
SmaI CCC'GGG
Cuts at: 1872 2968 1872
Size: 1096 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3745 4646 4908 820
Size: 197 926 1802 901 262 1553
Fragments arranged by size:
1802 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3432 493
Size: 2939 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5641
SphI G_CATG'C
Cuts at: 1132 2999 1132
Size: 1867 3774
SspI AAT'ATT
Cuts at: 5 52 3565 5
Size: 47 3513 2081
Fragments arranged by size:
3513 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5641
StyI C'CwwG_G
Cuts at: 513 1065 2004 2119 2226 3080 513
Size: 552 939 115 107 854 3074
Fragments arranged by size:
3074 939 854 552 115 107
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2562 3668 3685 3838 4023 4468 325
Size: 1255 982 1106 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1106 982 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 2245 2746
Size: 274 80 33 51 1443 204 64 501
Cuts at: 2746 3887 5599 96
Size: 1141 1712 138
Fragments arranged by size:
1712 1443 1141 501 274 204 138 80
64 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2118 2150 2309 2341 5601 5633 2118
Size: 32 159 32 3260 32 2126
Fragments arranged by size:
3260 2126 159 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5641
XbaI T'CTAG_A
Cuts at: 2977 2977
Size: 5641
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2626 2626
Size: 5641
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5641
XmaI C'CCGG_G
Cuts at: 1870 2966 1870
Size: 1096 4545
XmnI GAAnn'nnTTC
Cuts at: 3770 3770
Size: 5641
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3449 276
Size: 53 83 186 936 1915 2468
Fragments arranged by size:
2468 1915 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI