Restriction map for
pFN21AE5936
(Circular) (Six-base) MAPSORT of: pFN21AE5936.seq Check: 3723 from: 1 to: 5638
pFN21AE5936 5638 bp
With 182 enzymes: SgfI *
December 8, 2014 13:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3448 278
Size: 53 83 186 936 1912 2468
Fragments arranged by size:
2468 1912 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2598 2981 1937
Size: 661 383 4594
Fragments arranged by size:
4594 661 383
Acc65I G'GTAC_C
Cuts at: 2959 2959
Size: 5638
AclI AA'CG_TT
Cuts at: 1563 3765 4138 1563
Size: 2202 373 3063
Fragments arranged by size:
3063 2202 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2921 3701 5085 5353 1632
Size: 1289 780 1384 268 1917
Fragments arranged by size:
1917 1384 1289 780 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5441
AflIII A'CryG_T
Cuts at: 1203 2577 4537 5514 1203
Size: 1374 1960 977 1327
Fragments arranged by size:
1960 1374 1327 977
AgeI A'CCGG_T
Cuts at: 2367 4469 2367
Size: 2102 3536
AhdI GACnn_n'nnGTC
Cuts at: 4367 4367
Size: 5638
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5638
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5638
AlwNI CAG_nnn'CTG
Cuts at: 2123 2508 3381 4953 2123
Size: 385 873 1572 2808
Fragments arranged by size:
2808 1572 873 385
ApaLI G'TGCA_C
Cuts at: 2329 3695 4851 2329
Size: 1366 1156 3116
Fragments arranged by size:
3116 1366 1156
ApoI r'AATT_y
Cuts at: 1493 2947 3173 3325 5434 1493
Size: 1454 226 152 2109 1697
Fragments arranged by size:
2109 1697 1454 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4192 160
Size: 872 3160 1606
Fragments arranged by size:
3160 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5638
AvaI C'yCGr_G
Cuts at: 1870 1943 2784 2963 1870
Size: 73 841 179 4545
Fragments arranged by size:
4545 841 179 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2291 2324 2291
Size: 33 5605
BamHI G'GATC_C
Cuts at: 2968 3335 2968
Size: 367 5271
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2959 4414 618
Size: 325 217 627 1172 1455 1842
Fragments arranged by size:
1842 1455 1172 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2424 2957 1391
Size: 1033 533 4072
Fragments arranged by size:
4072 1033 533
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4667
BbvCI CC'TCA_GC
Cuts at: 2501 2501
Size: 5638
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3829 3863 1903
Size: 34 1892 34 3678
Fragments arranged by size:
3678 1892 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3530 4740 3530
Size: 1210 4428
BclI T'GATC_A
Cuts at: 1548 2133 1548
Size: 585 5053
BfrBI ATG'CAT
Cuts at: 2664 2685 2664
Size: 21 5617
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2357 4249 136
Size: 107 122 71 631 605 685 1892 1525
Fragments arranged by size:
1892 1525 685 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2481 2930 5633 2481
Size: 449 2703 2486
Fragments arranged by size:
2703 2486 449
BlpI GC'TnA_GC
Cuts at: 3055 3055
Size: 5638
Bme1580I G_kGCm'C
Cuts at: 1790 2241 2333 3699 4855 1790
Size: 451 92 1366 1156 2573
Fragments arranged by size:
2573 1366 1156 451 92
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5638
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3369 4327 442
Size: 416 53 83 399 24 1952 958 1753
Fragments arranged by size:
1952 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5638
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2535 4298 1239
Size: 1296 1763 2579
Fragments arranged by size:
2579 1763 1296
Bpu10I CC'TnA_GC
Cuts at: 2501 2501
Size: 5638
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2086 3763 4628 4926 2086
Size: 1677 865 298 2798
Fragments arranged by size:
2798 1677 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2207 4301 882
Size: 617 708 2094 2219
Fragments arranged by size:
2219 2094 708 617
BsaAI yAC'GTr
Cuts at: 493 1204 3429 493
Size: 711 2225 2702
Fragments arranged by size:
2702 2225 711
BsaBI GATnn'nnATC
Cuts at: 2138 5632 2138
Size: 3494 2144
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3445 3827 4462 275
Size: 53 83 186 936 1912 382 635 1451
Fragments arranged by size:
1912 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2367 4071 4469 4743 4890 5301 2367
Size: 1704 398 274 147 411 2704
Fragments arranged by size:
2704 1704 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2053 2083 2204 2234 2477
Size: 30 165 30 650 30 121 30 243
Cuts at: 2477 2507 1178
Size: 30 4309
Fragments arranged by size:
4309 650 243 165 121 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5638
BseYI C'CCAG_C
Cuts at: 4841 5337 4841
Size: 496 5142
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2153 2554 1163
Size: 573 417 401 4247
Fragments arranged by size:
4247 573 417 401
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3088 3849 4877 1540
Size: 275 186 1087 761 1028 2301
Fragments arranged by size:
2301 1087 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2333 3699 3784 4855 2333
Size: 1366 85 1071 3116
Fragments arranged by size:
3116 1366 1071 85
BsmI GAATG_Cn'
Cuts at: 2664 3149 3242 2664
Size: 485 93 5060
Fragments arranged by size:
5060 485 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2241 2333 2424 2957 3699 3784 4855
Size: 399 451 92 91 533 742 85 1071
Cuts at: 4855 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 533 451 399 92 91
85
BspEI T'CCGG_A
Cuts at: 5301 5301
Size: 5638
BspHI T'CATG_A
Cuts at: 3527 5257 3527
Size: 1730 3908
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2993 844
Size: 2149 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3525 1394
Size: 429 1702 3507
Fragments arranged by size:
3507 1702 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2316 4133 4307 65
Size: 1183 1068 1817 174 1396
Fragments arranged by size:
1817 1396 1183 1068 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2367 4282 4469 1700
Size: 110 557 1915 187 2869
Fragments arranged by size:
2869 1915 557 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5638
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5638
BssSI C'ACGA_G
Cuts at: 2025 3698 4710 2025
Size: 1673 1012 2953
Fragments arranged by size:
2953 1673 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5638
BstBI TT'CG_AA
Cuts at: 4442 4442
Size: 5638
BstEII G'GTnAC_C
Cuts at: 1184 2740 1184
Size: 1556 4082
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5638
BstYI r'GATC_y
Cuts at: 1972 2481 2930 2968 3335 3720 3737 5189 5275
Size: 509 449 38 367 385 17 1452 86
Cuts at: 5275 5287 5633 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 509 449 385 367 346 86
38 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2355 2416 513
Size: 552 939 351 61 3735
Fragments arranged by size:
3735 939 552 351 61
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4899
Fragments arranged by size:
4899 683 56
BtsI GCAGTG_nn'
Cuts at: 2165 3154 3948 3968 2165
Size: 989 794 20 3835
Fragments arranged by size:
3835 989 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5638
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5603
DraI TTT'AAA
Cuts at: 2942 2942
Size: 5638
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4645 809
Size: 507 3329 1802
Fragments arranged by size:
3329 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2162 2193 2349
Size: 54 1425 50 275 18 332 31 156
Cuts at: 2349 2376 2670 3085 3974 5603 8
Size: 27 294 415 889 1629 43
Fragments arranged by size:
1629 1425 889 415 332 294 275 156
54 50 43 31 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 3085 1537
Size: 275 1273 4090
Fragments arranged by size:
4090 1273 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3568 1353
Size: 2215 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2420 4225 4599 4745 387
Size: 1064 138 831 1805 374 146 1280
Fragments arranged by size:
1805 1280 1064 831 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2535 2921 3701 4298 5085 5353 1239
Size: 393 903 386 780 597 787 268 1524
Fragments arranged by size:
1524 903 787 780 597 393 386 268
EcoRI G'AATT_C
Cuts at: 2947 3325 2947
Size: 378 5260
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2503 2503
Size: 5638
FspI TGC'GCA
Cuts at: 4144 4144
Size: 5638
HaeII r_GCGC'y
Cuts at: 1678 4785 1678
Size: 3107 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4279 4311 4353 4385 1373
Size: 32 135 32 2707 32 42 32 2626
Fragments arranged by size:
2707 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2982 3228 669
Size: 988 281 1044 246 3079
Fragments arranged by size:
3079 1044 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5638
HpaI GTT'AAC
Cuts at: 3228 3228
Size: 5638
KpnI G_GTAC'C
Cuts at: 2963 2963
Size: 5638
MfeI C'AATT_G
Cuts at: 3237 3237
Size: 5638
MluI A'CGCG_T
Cuts at: 5514 5514
Size: 5638
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1992 4752 4936 1305
Size: 687 2760 184 2007
Fragments arranged by size:
2760 2007 687 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2195 2378 2672 10
Size: 54 1425 343 363 183 294 2976
Fragments arranged by size:
2976 1425 363 343 294 183 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2045 2228 2576 3596 3955 4114 518
Size: 648 879 183 348 1020 359 159 2042
Fragments arranged by size:
2042 1020 879 648 359 348 183 159
MspA1I CmG'CkG
Cuts at: 1606 3054 3731 4879 5124 1606
Size: 1448 677 1148 245 2120
Fragments arranged by size:
2120 1448 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2355 2416 513
Size: 552 939 351 61 3735
Fragments arranged by size:
3735 939 552 351 61
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5638
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5638
NotI GC'GGCC_GC
Cuts at: 3085 3085
Size: 5638
NsiI A_TGCA'T
Cuts at: 2666 2687 2666
Size: 21 5617
NspI r_CATG'y
Cuts at: 1132 1229 2581 2689 2996 4541 1132
Size: 97 1352 108 307 1545 2229
Fragments arranged by size:
2229 1545 1352 307 108 97
PasI CC'CwG_GG
Cuts at: 1348 2057 1348
Size: 709 4929
PciI A'CATG_T
Cuts at: 2577 4537 2577
Size: 1960 3678
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2734 1108
Size: 1626 4012
PmeI GTTT'AAAC
Cuts at: 2942 2942
Size: 5638
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3707 3739 5220 5252 3707
Size: 32 1481 32 4093
Fragments arranged by size:
4093 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2027 1613
Size: 414 5224
PsiI TTA'TAA
Cuts at: 3208 3208
Size: 5638
PspXI vC'TCGA_Gb
Cuts at: 2784 2784
Size: 5638
PstI C_TGCA'G
Cuts at: 830 2990 830
Size: 2160 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5638
SalI G'TCGA_C
Cuts at: 1936 2980 1936
Size: 1044 4594
SbfI CC_TGCA'GG
Cuts at: 2990 2990
Size: 5638
ScaI AGT'ACT
Cuts at: 2556 3886 5598 2556
Size: 1330 1712 2596
Fragments arranged by size:
2596 1712 1330
SexAI A'CCwGG_T
Cuts at: 2216 2216
Size: 5638
SfcI C'TryA_G
Cuts at: 826 1045 2986 4121 4802 4993 826
Size: 219 1941 1135 681 191 1471
Fragments arranged by size:
1941 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2357 2357
Size: 5638
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5638
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5638
SmaI CCC'GGG
Cuts at: 1872 2965 1872
Size: 1093 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2065 2784 3742 4643 4905 820
Size: 197 926 122 719 958 901 262 1553
Fragments arranged by size:
1553 958 926 901 719 262 197 122
SnaBI TAC'GTA
Cuts at: 493 3429 493
Size: 2936 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5638
SphI G_CATG'C
Cuts at: 1132 2689 2996 1132
Size: 1557 307 3774
Fragments arranged by size:
3774 1557 307
SspI AAT'ATT
Cuts at: 5 52 3562 5
Size: 47 3510 2081
Fragments arranged by size:
3510 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5638
StyI C'CwwG_G
Cuts at: 513 1065 2004 2355 2416 3077 513
Size: 552 939 351 61 661 3074
Fragments arranged by size:
3074 939 661 552 351 61
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3665 3682 3835 4020 4465 325
Size: 1255 2085 17 153 185 445 1498
Fragments arranged by size:
2085 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 2307 2554
Size: 274 80 33 51 1443 204 126 247
Cuts at: 2554 3884 5596 96
Size: 1330 1712 138
Fragments arranged by size:
1712 1443 1330 274 247 204 138 126
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5598 5630 5598
Size: 32 5606
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5638
XbaI T'CTAG_A
Cuts at: 2974 2974
Size: 5638
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2041 2590 2041
Size: 549 5089
XhoI C'TCGA_G
Cuts at: 1943 2784 1943
Size: 841 4797
XmaI C'CCGG_G
Cuts at: 1870 2963 1870
Size: 1093 4545
XmnI GAAnn'nnTTC
Cuts at: 2708 3767 2708
Size: 1059 4579
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3446 276
Size: 53 83 186 936 1912 2468
Fragments arranged by size:
2468 1912 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspXI PstI PvuI SalI SbfI ScaI SexAI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PpuMI PspOMI PsrI PvuII RsrII
SacI SacII SanDI SapI SfoI SrfI SwaI