Restriction map for
pFN21AE5933
(Circular) (Six-base) MAPSORT of: pFN21AE5933.seq Check: 2278 from: 1 to: 5638
pFN21AE5933 5638 bp
With 182 enzymes: SgfI *
December 8, 2014 13:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2448 2448
Size: 5638
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3448 278
Size: 53 83 186 936 1912 2468
Fragments arranged by size:
2468 1912 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2395 2981 1937
Size: 458 586 4594
Fragments arranged by size:
4594 586 458
Acc65I G'GTAC_C
Cuts at: 2509 2959 2509
Size: 450 5188
AclI AA'CG_TT
Cuts at: 1563 3765 4138 1563
Size: 2202 373 3063
Fragments arranged by size:
3063 2202 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2699 3701 5085 5353 1632
Size: 1067 1002 1384 268 1917
Fragments arranged by size:
1917 1384 1067 1002 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5441
AflIII A'CryG_T
Cuts at: 1203 2732 4537 5514 1203
Size: 1529 1805 977 1327
Fragments arranged by size:
1805 1529 1327 977
AgeI A'CCGG_T
Cuts at: 4469 4469
Size: 5638
AhdI GACnn_n'nnGTC
Cuts at: 4367 4367
Size: 5638
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5638
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5638
AlwNI CAG_nnn'CTG
Cuts at: 2257 3381 4953 2257
Size: 1124 1572 2942
Fragments arranged by size:
2942 1572 1124
ApaI G_GGCC'C
Cuts at: 2276 2276
Size: 5638
ApaLI G'TGCA_C
Cuts at: 3695 4851 3695
Size: 1156 4482
ApoI r'AATT_y
Cuts at: 1493 2809 2947 3173 3325 5434 1493
Size: 1316 138 226 152 2109 1697
Fragments arranged by size:
2109 1697 1316 226 152 138
AseI AT'TA_AT
Cuts at: 160 1032 4192 160
Size: 872 3160 1606
Fragments arranged by size:
3160 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5638
AvaI C'yCGr_G
Cuts at: 1870 1943 2963 1870
Size: 73 1020 4545
Fragments arranged by size:
4545 1020 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2501 2534 2577 2610 2501
Size: 33 43 33 5529
Fragments arranged by size:
5529 43 33 33
BamHI G'GATC_C
Cuts at: 2968 3335 2968
Size: 367 5271
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2509 2669 2959 4414 618
Size: 325 217 627 722 160 290 1455 1842
Fragments arranged by size:
1842 1455 722 627 325 290 217 160
BanII G_rGCy'C
Cuts at: 1391 2276 2743 2957 1391
Size: 885 467 214 4072
Fragments arranged by size:
4072 885 467 214
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4667
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3829 3863 1903
Size: 34 1892 34 3678
Fragments arranged by size:
3678 1892 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3530 4740 3530
Size: 1210 4428
BclI T'GATC_A
Cuts at: 1548 2613 1548
Size: 1065 4573
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4249 136
Size: 107 122 71 631 605 2577 1525
Fragments arranged by size:
2577 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5633 5633
Size: 5638
BlpI GC'TnA_GC
Cuts at: 3055 3055
Size: 5638
Bme1580I G_kGCm'C
Cuts at: 1790 2276 3699 4855 1790
Size: 486 1423 1156 2573
Fragments arranged by size:
2573 1423 1156 486
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5638
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2342 2718 3369
Size: 416 53 83 399 24 925 376 651
Cuts at: 3369 4327 442
Size: 958 1753
Fragments arranged by size:
1753 958 925 651 416 399 376 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2034 1056
Size: 978 4660
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2777 4298 1239
Size: 1538 1521 2579
Fragments arranged by size:
2579 1538 1521
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2799 3763 4628 4926 2799
Size: 964 865 298 3511
Fragments arranged by size:
3511 964 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4301 882
Size: 617 2802 2219
Fragments arranged by size:
2802 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3429 493
Size: 711 2225 2702
Fragments arranged by size:
2702 2225 711
BsaBI GATnn'nnATC
Cuts at: 5632 5632
Size: 5638
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3445 3827 4462 275
Size: 53 83 186 936 1912 382 635 1451
Fragments arranged by size:
1912 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4071 4469 4743 4890 5301 4071
Size: 398 274 147 411 4408
Fragments arranged by size:
4408 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2153 2183 1178
Size: 30 165 30 750 30 4633
Fragments arranged by size:
4633 750 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5638
BseYI C'CCAG_C
Cuts at: 4841 5337 4841
Size: 496 5142
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2162 1163
Size: 573 426 4639
Fragments arranged by size:
4639 573 426
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3088 3849 4877 1540
Size: 275 186 1087 761 1028 2301
Fragments arranged by size:
2301 1087 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2743 3699 3784 4855 2743
Size: 956 85 1071 3526
Fragments arranged by size:
3526 1071 956 85
BsmI GAATG_Cn'
Cuts at: 3149 3242 3149
Size: 93 5545
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2276 2743 2957 3699 3784 4855 1391
Size: 399 486 467 214 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 486 467 399 214 85
BspEI T'CCGG_A
Cuts at: 5301 5301
Size: 5638
BspHI T'CATG_A
Cuts at: 2610 3527 5257 2610
Size: 917 1730 2991
Fragments arranged by size:
2991 1730 917
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2448 2993 844
Size: 1604 545 3489
Fragments arranged by size:
3489 1604 545
BsrBI CCG'CTC
Cuts at: 1394 1823 3525 1394
Size: 429 1702 3507
Fragments arranged by size:
3507 1702 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2832 4133 4307 65
Size: 1183 1584 1301 174 1396
Fragments arranged by size:
1584 1396 1301 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4282 4469 1700
Size: 110 2472 187 2869
Fragments arranged by size:
2869 2472 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5638
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5638
BssSI C'ACGA_G
Cuts at: 3698 4710 3698
Size: 1012 4626
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5638
BstBI TT'CG_AA
Cuts at: 4442 4442
Size: 5638
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5638
BstYI r'GATC_y
Cuts at: 1972 2221 2968 3335 3720 3737 5189 5275 5287
Size: 249 747 367 385 17 1452 86 12
Cuts at: 5287 5633 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 747 385 367 346 249 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5086
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4899
Fragments arranged by size:
4899 683 56
BtsI GCAGTG_nn'
Cuts at: 2919 3154 3948 3968 2919
Size: 235 794 20 4589
Fragments arranged by size:
4589 794 235 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5638
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5603
DraI TTT'AAA
Cuts at: 2942 2942
Size: 5638
DraIII CAC_nnn'GTG
Cuts at: 2346 2346
Size: 5638
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4645 809
Size: 507 3329 1802
Fragments arranged by size:
3329 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2775 3085 3974
Size: 54 1425 50 275 18 945 310 889
Cuts at: 3974 5603 8
Size: 1629 43
Fragments arranged by size:
1629 1425 945 889 310 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3085 1537
Size: 275 1273 4090
Fragments arranged by size:
4090 1273 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2912 3568 1353
Size: 1559 656 3423
Fragments arranged by size:
3423 1559 656
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4225 4599 4745 387
Size: 1064 138 2636 374 146 1280
Fragments arranged by size:
2636 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2741 2741
Size: 5638
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2699 2777 3701 4298 5085 5353 1239
Size: 393 1067 78 924 597 787 268 1524
Fragments arranged by size:
1524 1067 924 787 597 393 268 78
EcoO109I rG'GnC_Cy
Cuts at: 2272 2272
Size: 5638
EcoRI G'AATT_C
Cuts at: 2947 3325 2947
Size: 378 5260
FspI TGC'GCA
Cuts at: 4144 4144
Size: 5638
HaeII r_GCGC'y
Cuts at: 1678 4785 1678
Size: 3107 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4279 4311 4353 4385 1373
Size: 32 135 32 2707 32 42 32 2626
Fragments arranged by size:
2707 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2703 2982 3228 669
Size: 988 281 765 279 246 3079
Fragments arranged by size:
3079 988 765 281 279 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5638
HpaI GTT'AAC
Cuts at: 3228 3228
Size: 5638
KpnI G_GTAC'C
Cuts at: 2513 2963 2513
Size: 450 5188
MfeI C'AATT_G
Cuts at: 3237 3237
Size: 5638
MluI A'CGCG_T
Cuts at: 5514 5514
Size: 5638
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2038 4752 4936 1305
Size: 733 2714 184 2007
Fragments arranged by size:
2714 2007 733 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3816
Fragments arranged by size:
3816 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2186 2595 3596 3955 4114 518
Size: 648 1020 409 1001 359 159 2042
Fragments arranged by size:
2042 1020 1001 648 409 359 159
MspA1I CmG'CkG
Cuts at: 1606 2387 3054 3731 4879 5124 1606
Size: 781 667 677 1148 245 2120
Fragments arranged by size:
2120 1148 781 677 667 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5086
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5638
NheI G'CTAG_C
Cuts at: 1052 2030 1052
Size: 978 4660
NotI GC'GGCC_GC
Cuts at: 3085 3085
Size: 5638
NspI r_CATG'y
Cuts at: 1132 1229 2736 2996 4541 1132
Size: 97 1507 260 1545 2229
Fragments arranged by size:
2229 1545 1507 260 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5638
PciI A'CATG_T
Cuts at: 2732 4537 2732
Size: 1805 3833
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2443 1108
Size: 1335 4303
PmeI GTTT'AAAC
Cuts at: 2942 2942
Size: 5638
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3707 3739 5220 5252 3707
Size: 32 1481 32 4093
Fragments arranged by size:
4093 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5638
PsiI TTA'TAA
Cuts at: 3208 3208
Size: 5638
PspOMI G'GGCC_C
Cuts at: 2272 2272
Size: 5638
PstI C_TGCA'G
Cuts at: 830 2990 830
Size: 2160 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5638
PvuII CAG'CTG
Cuts at: 2387 2387
Size: 5638
SacI G_AGCT'C
Cuts at: 2743 2743
Size: 5638
SalI G'TCGA_C
Cuts at: 1936 2980 1936
Size: 1044 4594
SbfI CC_TGCA'GG
Cuts at: 2990 2990
Size: 5638
ScaI AGT'ACT
Cuts at: 3886 5598 3886
Size: 1712 3926
SfcI C'TryA_G
Cuts at: 826 1045 2986 4121 4802 4993 826
Size: 219 1941 1135 681 191 1471
Fragments arranged by size:
1941 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5638
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5638
SmaI CCC'GGG
Cuts at: 1872 2965 1872
Size: 1093 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2814 3742 4643 4905 820
Size: 197 926 871 928 901 262 1553
Fragments arranged by size:
1553 928 926 901 871 262 197
SnaBI TAC'GTA
Cuts at: 493 3429 493
Size: 2936 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5638
SphI G_CATG'C
Cuts at: 1132 2996 1132
Size: 1864 3774
SspI AAT'ATT
Cuts at: 5 52 3562 5
Size: 47 3510 2081
Fragments arranged by size:
3510 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5638
StyI C'CwwG_G
Cuts at: 513 1065 3077 513
Size: 552 2012 3074
Fragments arranged by size:
3074 2012 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3665 3682 3835 4020 4465 325
Size: 1255 2085 17 153 185 445 1498
Fragments arranged by size:
2085 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2190 2686 3884
Size: 274 80 33 51 1443 213 496 1198
Cuts at: 3884 5596 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1198 496 274 213 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5598 5630 5598
Size: 32 5606
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5638
XbaI T'CTAG_A
Cuts at: 2974 2974
Size: 5638
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5638
XmaI C'CCGG_G
Cuts at: 1870 2963 1870
Size: 1093 4545
XmnI GAAnn'nnTTC
Cuts at: 3767 3767
Size: 5638
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3446 276
Size: 53 83 186 936 1912 2468
Fragments arranged by size:
2468 1912 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI