Restriction map for
pFN21AE5931
(Circular) (Six-base) MAPSORT of: pFN21AE5931.seq Check: 9320 from: 1 to: 5638
pFN21AE5931 5638 bp
With 182 enzymes: SgfI *
December 8, 2014 13:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3448 278
Size: 53 83 186 936 1912 2468
Fragments arranged by size:
2468 1912 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2448 2981 1937
Size: 511 533 4594
Fragments arranged by size:
4594 533 511
Acc65I G'GTAC_C
Cuts at: 2959 2959
Size: 5638
AclI AA'CG_TT
Cuts at: 1563 3765 4138 1563
Size: 2202 373 3063
Fragments arranged by size:
3063 2202 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2010 2927 3701 5085 5353 1632
Size: 378 917 774 1384 268 1917
Fragments arranged by size:
1917 1384 917 774 378 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5441
AflIII A'CryG_T
Cuts at: 1203 4537 5514 1203
Size: 3334 977 1327
Fragments arranged by size:
3334 1327 977
AgeI A'CCGG_T
Cuts at: 4469 4469
Size: 5638
AhdI GACnn_n'nnGTC
Cuts at: 4367 4367
Size: 5638
AleI CACnn'nnGTG
Cuts at: 1166 2120 1166
Size: 954 4684
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5638
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2264 2296 2264
Size: 32 5606
AlwNI CAG_nnn'CTG
Cuts at: 2033 2424 3381 4953 2033
Size: 391 957 1572 2718
Fragments arranged by size:
2718 1572 957 391
ApaLI G'TGCA_C
Cuts at: 3695 4851 3695
Size: 1156 4482
ApoI r'AATT_y
Cuts at: 1493 2947 3173 3325 5434 1493
Size: 1454 226 152 2109 1697
Fragments arranged by size:
2109 1697 1454 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4192 160
Size: 872 3160 1606
Fragments arranged by size:
3160 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5638
AvaI C'yCGr_G
Cuts at: 1870 1943 2963 1870
Size: 73 1020 4545
Fragments arranged by size:
4545 1020 73
BamHI G'GATC_C
Cuts at: 2968 3335 2968
Size: 367 5271
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2959 4414 618
Size: 325 217 627 1172 1455 1842
Fragments arranged by size:
1842 1455 1172 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2957 1391
Size: 1566 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2817 928
Size: 971 918 3749
Fragments arranged by size:
3749 971 918
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3829 3863 1903
Size: 34 1892 34 3678
Fragments arranged by size:
3678 1892 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2687 3530 4740 2687
Size: 843 1210 3585
Fragments arranged by size:
3585 1210 843
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5638
BfrBI ATG'CAT
Cuts at: 2295 2295
Size: 5638
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4249 136
Size: 107 122 71 631 605 2577 1525
Fragments arranged by size:
2577 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5633 5633
Size: 5638
BlpI GC'TnA_GC
Cuts at: 3055 3055
Size: 5638
Bme1580I G_kGCm'C
Cuts at: 1790 3699 4855 1790
Size: 1909 1156 2573
Fragments arranged by size:
2573 1909 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5638
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2465 3369 4327
Size: 416 53 83 399 24 1048 904 958
Cuts at: 4327 442
Size: 1753
Fragments arranged by size:
1753 1048 958 904 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5638
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2160 2557 4298 1239
Size: 921 397 1741 2579
Fragments arranged by size:
2579 1741 921 397
Bpu10I CC'TnA_GC
Cuts at: 2626 2626
Size: 5638
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3763 4628 4926 3763
Size: 865 298 4475
Fragments arranged by size:
4475 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2520 4301 882
Size: 617 1021 1781 2219
Fragments arranged by size:
2219 1781 1021 617
BsaAI yAC'GTr
Cuts at: 493 1204 3429 493
Size: 711 2225 2702
Fragments arranged by size:
2702 2225 711
BsaBI GATnn'nnATC
Cuts at: 5632 5632
Size: 5638
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3445 3827 4462 275
Size: 53 83 186 936 1912 382 635 1451
Fragments arranged by size:
1912 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4071 4469 4743 4890 5301 4071
Size: 398 274 147 411 4408
Fragments arranged by size:
4408 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2073 2103 2264 2294 1178
Size: 30 165 30 670 30 161 30 4522
Fragments arranged by size:
4522 670 165 161 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2215 2282 2628 1186
Size: 1029 67 346 4196
Fragments arranged by size:
4196 1029 346 67
BseYI C'CCAG_C
Cuts at: 4841 5337 4841
Size: 496 5142
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5065
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3088 3849 4877 1540
Size: 275 186 1087 761 1028 2301
Fragments arranged by size:
2301 1087 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3699 3784 4855 3699
Size: 85 1071 4482
Fragments arranged by size:
4482 1071 85
BsmI GAATG_Cn'
Cuts at: 2297 3149 3242 2297
Size: 852 93 4693
Fragments arranged by size:
4693 852 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2957 3699 3784 4855 1391
Size: 399 1167 742 85 1071 2174
Fragments arranged by size:
2174 1167 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5301 5301
Size: 5638
BspHI T'CATG_A
Cuts at: 3527 5257 3527
Size: 1730 3908
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2993 844
Size: 2149 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3525 1394
Size: 429 1702 3507
Fragments arranged by size:
3507 1702 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4133 4307 65
Size: 1183 2885 174 1396
Fragments arranged by size:
2885 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4282 4469 1700
Size: 110 2472 187 2869
Fragments arranged by size:
2869 2472 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5638
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5638
BssSI C'ACGA_G
Cuts at: 3698 4710 3698
Size: 1012 4626
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5638
BstBI TT'CG_AA
Cuts at: 4442 4442
Size: 5638
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5638
BstXI CCAn_nnnn'nTGG
Cuts at: 2437 2437
Size: 5638
BstYI r'GATC_y
Cuts at: 1972 2968 3335 3720 3737 5189 5275 5287 5633
Size: 996 367 385 17 1452 86 12 346
Cuts at: 5633 1972
Size: 1977
Fragments arranged by size:
1977 1452 996 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2449 2449
Size: 5638
Bsu36I CC'TnA_GG
Cuts at: 2879 2879
Size: 5638
BtgI C'CryG_G
Cuts at: 513 1065 2004 2355 513
Size: 552 939 351 3796
Fragments arranged by size:
3796 939 552 351
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4899
Fragments arranged by size:
4899 683 56
BtsI GCAGTG_nn'
Cuts at: 2390 3154 3948 3968 2390
Size: 764 794 20 4060
Fragments arranged by size:
4060 794 764 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5638
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5603
DraI TTT'AAA
Cuts at: 2942 2942
Size: 5638
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4645 809
Size: 507 3329 1802
Fragments arranged by size:
3329 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3085 3974 5603
Size: 54 1425 50 275 18 1255 889 1629
Cuts at: 5603 8
Size: 43
Fragments arranged by size:
1629 1425 1255 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3085 1537
Size: 275 1273 4090
Fragments arranged by size:
4090 1273 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2090 3568 1353
Size: 737 1478 3423
Fragments arranged by size:
3423 1478 737
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4225 4599 4745 387
Size: 1064 138 2636 374 146 1280
Fragments arranged by size:
2636 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2010 2160 2557 2927 3701 4298 5085
Size: 393 378 150 397 370 774 597 787
Cuts at: 5085 5353 1239
Size: 268 1524
Fragments arranged by size:
1524 787 774 597 397 393 378 370
268 150
EcoRI G'AATT_C
Cuts at: 2947 3325 2947
Size: 378 5260
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2360 2360
Size: 5638
FspI TGC'GCA
Cuts at: 4144 4144
Size: 5638
HaeII r_GCGC'y
Cuts at: 1678 4785 1678
Size: 3107 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2690 2722 2807 2839 4279
Size: 32 135 32 1118 32 85 32 1440
Cuts at: 4279 4311 4353 4385 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1440 1118 135 85 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2982 3228 669
Size: 988 281 1044 246 3079
Fragments arranged by size:
3079 1044 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5638
HpaI GTT'AAC
Cuts at: 3228 3228
Size: 5638
KpnI G_GTAC'C
Cuts at: 2963 2963
Size: 5638
MfeI C'AATT_G
Cuts at: 3237 3237
Size: 5638
MluI A'CGCG_T
Cuts at: 5514 5514
Size: 5638
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4752 4936 1305
Size: 3447 184 2007
Fragments arranged by size:
3447 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3816
Fragments arranged by size:
3816 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2120 2435 2495 2586 2804 3596 3955
Size: 648 954 315 60 91 218 792 359
Cuts at: 3955 4114 518
Size: 159 2042
Fragments arranged by size:
2042 954 792 648 359 315 218 159
91 60
MspA1I CmG'CkG
Cuts at: 1606 3054 3731 4879 5124 1606
Size: 1448 677 1148 245 2120
Fragments arranged by size:
2120 1448 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2355 513
Size: 552 939 351 3796
Fragments arranged by size:
3796 939 552 351
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5638
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5638
NotI GC'GGCC_GC
Cuts at: 3085 3085
Size: 5638
NsiI A_TGCA'T
Cuts at: 2297 2297
Size: 5638
NspI r_CATG'y
Cuts at: 1132 1229 2494 2996 4541 1132
Size: 97 1265 502 1545 2229
Fragments arranged by size:
2229 1545 1265 502 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5638
PciI A'CATG_T
Cuts at: 4537 4537
Size: 5638
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2740 1108
Size: 1632 4006
PmeI GTTT'AAAC
Cuts at: 2942 2942
Size: 5638
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2264 2296 3707 3739 5220 5252 2264
Size: 32 1411 32 1481 32 2650
Fragments arranged by size:
2650 1481 1411 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5638
PsiI TTA'TAA
Cuts at: 3208 3208
Size: 5638
PstI C_TGCA'G
Cuts at: 830 2990 830
Size: 2160 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5638
SalI G'TCGA_C
Cuts at: 1936 2980 1936
Size: 1044 4594
SbfI CC_TGCA'GG
Cuts at: 2990 2990
Size: 5638
ScaI AGT'ACT
Cuts at: 3886 5598 3886
Size: 1712 3926
SfcI C'TryA_G
Cuts at: 826 1045 2509 2872 2986 4121 4802 4993 826
Size: 219 1464 363 114 1135 681 191 1471
Fragments arranged by size:
1471 1464 1135 681 363 219 191 114
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5638
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5638
SmaI CCC'GGG
Cuts at: 1872 2965 1872
Size: 1093 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3742 4643 4905 820
Size: 197 926 1799 901 262 1553
Fragments arranged by size:
1799 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3429 493
Size: 2936 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5638
SphI G_CATG'C
Cuts at: 1132 2996 1132
Size: 1864 3774
SspI AAT'ATT
Cuts at: 5 52 2404 3562 5
Size: 47 2352 1158 2081
Fragments arranged by size:
2352 2081 1158 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5638
StyI C'CwwG_G
Cuts at: 513 1065 2004 2098 2355 2680 3077 513
Size: 552 939 94 257 325 397 3074
Fragments arranged by size:
3074 939 552 397 325 257 94
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2594 3665 3682 3835 4020 4465 325
Size: 1255 1014 1071 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1071 1014 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3884 5596 96
Size: 274 80 33 51 1443 1907 1712 138
Fragments arranged by size:
1907 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2073 2094 2105 2126 2628 2660 5598 5630 2073
Size: 21 11 21 502 32 2938 32 2081
Fragments arranged by size:
2938 2081 502 32 32 21 21 11
Tth111I GACn'n_nGTC
Cuts at: 1316 2563 1316
Size: 1247 4391
XbaI T'CTAG_A
Cuts at: 2974 2974
Size: 5638
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2803 2803
Size: 5638
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5638
XmaI C'CCGG_G
Cuts at: 1870 2963 1870
Size: 1093 4545
XmnI GAAnn'nnTTC
Cuts at: 3767 3767
Size: 5638
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3446 276
Size: 53 83 186 936 1912 2468
Fragments arranged by size:
2468 1912 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BplI BsiWI BsmBI DraIII EcoICRI EcoNI EcoO109I EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI