Restriction map for
pFN21AE5924
(Circular) (Six-base) MAPSORT of: pFN21AE5924.seq Check: 3700 from: 1 to: 5638
pFN21AE5924 5638 bp
With 182 enzymes: SgfI *
December 8, 2014 13:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3448 278
Size: 53 83 186 936 1912 2468
Fragments arranged by size:
2468 1912 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2981 1937
Size: 1044 4594
Acc65I G'GTAC_C
Cuts at: 2959 2959
Size: 5638
AclI AA'CG_TT
Cuts at: 1563 3765 4138 1563
Size: 2202 373 3063
Fragments arranged by size:
3063 2202 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2576 3701 5085 5353 1632
Size: 944 1125 1384 268 1917
Fragments arranged by size:
1917 1384 1125 944 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5441
AflIII A'CryG_T
Cuts at: 1203 4537 5514 1203
Size: 3334 977 1327
Fragments arranged by size:
3334 1327 977
AgeI A'CCGG_T
Cuts at: 4469 4469
Size: 5638
AhdI GACnn_n'nnGTC
Cuts at: 4367 4367
Size: 5638
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5638
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5638
AlwNI CAG_nnn'CTG
Cuts at: 2248 2434 3381 4953 2248
Size: 186 947 1572 2933
Fragments arranged by size:
2933 1572 947 186
ApaLI G'TGCA_C
Cuts at: 2657 3695 4851 2657
Size: 1038 1156 3444
Fragments arranged by size:
3444 1156 1038
ApoI r'AATT_y
Cuts at: 1493 2816 2947 3173 3325 5434 1493
Size: 1323 131 226 152 2109 1697
Fragments arranged by size:
2109 1697 1323 226 152 131
AseI AT'TA_AT
Cuts at: 160 1032 4192 160
Size: 872 3160 1606
Fragments arranged by size:
3160 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5638
AvaI C'yCGr_G
Cuts at: 1870 1943 2607 2963 1870
Size: 73 664 356 4545
Fragments arranged by size:
4545 664 356 73
BamHI G'GATC_C
Cuts at: 2968 3335 2968
Size: 367 5271
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2959 4414 618
Size: 325 217 627 1172 1455 1842
Fragments arranged by size:
1842 1455 1172 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2079 2330 2902 2957 1391
Size: 688 251 572 55 4072
Fragments arranged by size:
4072 688 572 251 55
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4667
BbvCI CC'TCA_GC
Cuts at: 2678 2678
Size: 5638
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3829 3863 1903
Size: 34 1892 34 3678
Fragments arranged by size:
3678 1892 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3530 4740 3530
Size: 1210 4428
BclI T'GATC_A
Cuts at: 1548 2250 1548
Size: 702 4936
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4249 136
Size: 107 122 71 631 605 2577 1525
Fragments arranged by size:
2577 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5633 5633
Size: 5638
BlpI GC'TnA_GC
Cuts at: 2301 2761 3055 2301
Size: 460 294 4884
Fragments arranged by size:
4884 460 294
Bme1580I G_kGCm'C
Cuts at: 1790 2241 2661 3699 4855 1790
Size: 451 420 1038 1156 2573
Fragments arranged by size:
2573 1156 1038 451 420
BmgBI CAC'GTC
Cuts at: 1318 2890 1318
Size: 1572 4066
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3369 4327 442
Size: 416 53 83 399 24 1952 958 1753
Fragments arranged by size:
1952 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5638
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2093 4298 1239
Size: 854 2205 2579
Fragments arranged by size:
2579 2205 854
Bpu10I CC'TnA_GC
Cuts at: 2678 2678
Size: 5638
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3763 4628 4926 3763
Size: 865 298 4475
Fragments arranged by size:
4475 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2492 4301 882
Size: 617 993 1809 2219
Fragments arranged by size:
2219 1809 993 617
BsaAI yAC'GTr
Cuts at: 493 1204 3429 493
Size: 711 2225 2702
Fragments arranged by size:
2702 2225 711
BsaBI GATnn'nnATC
Cuts at: 5632 5632
Size: 5638
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3445 3827 4462 275
Size: 53 83 186 936 1912 382 635 1451
Fragments arranged by size:
1912 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4071 4469 4743 4890 5301 4071
Size: 398 274 147 411 4408
Fragments arranged by size:
4408 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2280 2310 1178
Size: 30 165 30 877 30 4506
Fragments arranged by size:
4506 877 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5638
BseYI C'CCAG_C
Cuts at: 2241 2696 4841 5337 2241
Size: 455 2145 496 2542
Fragments arranged by size:
2542 2145 496 455
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2153 1163
Size: 573 417 4648
Fragments arranged by size:
4648 573 417
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3088 3849 4877 1540
Size: 275 186 1087 761 1028 2301
Fragments arranged by size:
2301 1087 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2346 2661 3699 3784 4855 2346
Size: 315 1038 85 1071 3129
Fragments arranged by size:
3129 1071 1038 315 85
BsmI GAATG_Cn'
Cuts at: 3149 3242 3149
Size: 93 5545
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2079 2241 2330 2346 2661 2902 2957
Size: 399 289 162 89 16 315 241 55
Cuts at: 2957 3699 3784 4855 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 315 289 241 162
89 85 55 16
BspEI T'CCGG_A
Cuts at: 5301 5301
Size: 5638
BspHI T'CATG_A
Cuts at: 3527 5257 3527
Size: 1730 3908
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2993 844
Size: 2149 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3525 1394
Size: 429 1702 3507
Fragments arranged by size:
3507 1702 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2499 2661 4133 4307 65
Size: 1183 1251 162 1472 174 1396
Fragments arranged by size:
1472 1396 1251 1183 174 162
BsrFI r'CCGG_y
Cuts at: 1700 1810 4282 4469 1700
Size: 110 2472 187 2869
Fragments arranged by size:
2869 2472 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5638
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5638
BssSI C'ACGA_G
Cuts at: 3698 4710 3698
Size: 1012 4626
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5638
BstBI TT'CG_AA
Cuts at: 4442 4442
Size: 5638
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5638
BstYI r'GATC_y
Cuts at: 1972 2968 3335 3720 3737 5189 5275 5287 5633
Size: 996 367 385 17 1452 86 12 346
Cuts at: 5633 1972
Size: 1977
Fragments arranged by size:
1977 1452 996 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2284 513
Size: 552 939 280 3867
Fragments arranged by size:
3867 939 552 280
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2125 487
Size: 683 56 899 4000
Fragments arranged by size:
4000 899 683 56
BtsI GCAGTG_nn'
Cuts at: 2679 3154 3948 3968 2679
Size: 475 794 20 4349
Fragments arranged by size:
4349 794 475 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5638
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5603
DraI TTT'AAA
Cuts at: 2942 2942
Size: 5638
DraIII CAC_nnn'GTG
Cuts at: 2337 2337
Size: 5638
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2725 4645 809
Size: 507 1409 1920 1802
Fragments arranged by size:
1920 1802 1409 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2063 2403 2496
Size: 54 1425 50 275 18 233 340 93
Cuts at: 2496 2512 3085 3974 5603 8
Size: 16 573 889 1629 43
Fragments arranged by size:
1629 1425 889 573 340 275 233 93
54 50 43 18 16
EagI C'GGCC_G
Cuts at: 1537 1812 3085 1537
Size: 275 1273 4090
Fragments arranged by size:
4090 1273 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2312 3568 1353
Size: 959 1256 3423
Fragments arranged by size:
3423 1256 959
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4225 4599 4745 387
Size: 1064 138 2636 374 146 1280
Fragments arranged by size:
2636 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2093 2576 3701 4298 5085 5353 1239
Size: 393 461 483 1125 597 787 268 1524
Fragments arranged by size:
1524 1125 787 597 483 461 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2727 2727
Size: 5638
EcoRI G'AATT_C
Cuts at: 2947 3325 2947
Size: 378 5260
FspI TGC'GCA
Cuts at: 4144 4144
Size: 5638
HaeII r_GCGC'y
Cuts at: 1678 4785 1678
Size: 3107 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2601 2633 4279 4311 4353
Size: 32 135 32 1029 32 1646 32 42
Cuts at: 4353 4385 1373
Size: 32 2626
Fragments arranged by size:
2626 1646 1029 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2691 2982 3228 669
Size: 988 281 753 291 246 3079
Fragments arranged by size:
3079 988 753 291 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5638
HpaI GTT'AAC
Cuts at: 3228 3228
Size: 5638
KpnI G_GTAC'C
Cuts at: 2963 2963
Size: 5638
MfeI C'AATT_G
Cuts at: 3237 3237
Size: 5638
MluI A'CGCG_T
Cuts at: 5514 5514
Size: 5638
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4752 4936 1305
Size: 3447 184 2007
Fragments arranged by size:
3447 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2405 2498 2514 10
Size: 54 1425 343 573 93 16 3134
Fragments arranged by size:
3134 1425 573 343 93 54 16
MslI CAynn'nnrTG
Cuts at: 518 1166 2045 2177 2618 3596 3955 4114 518
Size: 648 879 132 441 978 359 159 2042
Fragments arranged by size:
2042 978 879 648 441 359 159 132
MspA1I CmG'CkG
Cuts at: 1606 3054 3731 4879 5124 1606
Size: 1448 677 1148 245 2120
Fragments arranged by size:
2120 1448 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4147
Fragments arranged by size:
4147 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5638
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5638
NotI GC'GGCC_GC
Cuts at: 3085 3085
Size: 5638
NspI r_CATG'y
Cuts at: 1132 1229 2996 4541 1132
Size: 97 1767 1545 2229
Fragments arranged by size:
2229 1767 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2323 1348
Size: 975 4663
PciI A'CATG_T
Cuts at: 4537 4537
Size: 5638
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2401 2434 1108
Size: 1293 33 4312
Fragments arranged by size:
4312 1293 33
PfoI T'CCnGG_A
Cuts at: 2208 2208
Size: 5638
PmeI GTTT'AAAC
Cuts at: 2942 2942
Size: 5638
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3707 3739 5220 5252 3707
Size: 32 1481 32 4093
Fragments arranged by size:
4093 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2727 2727
Size: 5638
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5638
PsiI TTA'TAA
Cuts at: 3208 3208
Size: 5638
PstI C_TGCA'G
Cuts at: 830 2635 2990 830
Size: 1805 355 3478
Fragments arranged by size:
3478 1805 355
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5638
SalI G'TCGA_C
Cuts at: 1936 2980 1936
Size: 1044 4594
SanDI GG'GwC_CC
Cuts at: 2727 2727
Size: 5638
SapI GCTCTTCn'nnn_
Cuts at: 2312 2312
Size: 5638
SbfI CC_TGCA'GG
Cuts at: 2990 2990
Size: 5638
ScaI AGT'ACT
Cuts at: 2913 3886 5598 2913
Size: 973 1712 2953
Fragments arranged by size:
2953 1712 973
SfcI C'TryA_G
Cuts at: 826 1045 2631 2986 4121 4802 4993 826
Size: 219 1586 355 1135 681 191 1471
Fragments arranged by size:
1586 1471 1135 681 355 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5638
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5638
SmaI CCC'GGG
Cuts at: 1872 2965 1872
Size: 1093 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3742 4643 4905 820
Size: 197 926 1799 901 262 1553
Fragments arranged by size:
1799 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3429 493
Size: 2936 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5638
SphI G_CATG'C
Cuts at: 1132 2996 1132
Size: 1864 3774
SspI AAT'ATT
Cuts at: 5 52 3562 5
Size: 47 3510 2081
Fragments arranged by size:
3510 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5638
StyI C'CwwG_G
Cuts at: 513 1065 2004 3077 513
Size: 552 939 1073 3074
Fragments arranged by size:
3074 1073 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3665 3682 3835 4020 4465 325
Size: 1255 2085 17 153 185 445 1498
Fragments arranged by size:
2085 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 2911 3884
Size: 274 80 33 51 1443 204 730 973
Cuts at: 3884 5596 96
Size: 1712 138
Fragments arranged by size:
1712 1443 973 730 274 204 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2280 2312 5598 5630 2280
Size: 32 3286 32 2288
Fragments arranged by size:
3286 2288 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5638
XbaI T'CTAG_A
Cuts at: 2974 2974
Size: 5638
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2599 2599
Size: 5638
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5638
XmaI C'CCGG_G
Cuts at: 1870 2963 1870
Size: 1093 4545
XmnI GAAnn'nnTTC
Cuts at: 2528 3767 2528
Size: 1239 4399
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3446 276
Size: 53 83 186 936 1912 2468
Fragments arranged by size:
2468 1912 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SalI SanDI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SexAI SfiI SfoI SrfI
SwaI