Restriction map for
pFN21AE5921
(Circular) (Six-base) MAPSORT of: pFN21AE5921.seq Check: 2592 from: 1 to: 5638
pFN21AE5921 5638 bp
With 182 enzymes: SgfI *
December 8, 2014 13:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3448 278
Size: 53 83 186 936 1912 2468
Fragments arranged by size:
2468 1912 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2981 1937
Size: 1044 4594
Acc65I G'GTAC_C
Cuts at: 2959 2959
Size: 5638
AclI AA'CG_TT
Cuts at: 1563 3765 4138 1563
Size: 2202 373 3063
Fragments arranged by size:
3063 2202 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3701 5085 5353 1632
Size: 2069 1384 268 1917
Fragments arranged by size:
2069 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5441
AflIII A'CryG_T
Cuts at: 1203 4537 5514 1203
Size: 3334 977 1327
Fragments arranged by size:
3334 1327 977
AgeI A'CCGG_T
Cuts at: 4469 4469
Size: 5638
AhdI GACnn_n'nnGTC
Cuts at: 4367 4367
Size: 5638
AleI CACnn'nnGTG
Cuts at: 1166 2123 2747 2846 1166
Size: 957 624 99 3958
Fragments arranged by size:
3958 957 624 99
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5638
AlwNI CAG_nnn'CTG
Cuts at: 3381 4953 3381
Size: 1572 4066
ApaLI G'TGCA_C
Cuts at: 3695 4851 3695
Size: 1156 4482
ApoI r'AATT_y
Cuts at: 1493 2257 2928 2947 3173 3325 5434 1493
Size: 764 671 19 226 152 2109 1697
Fragments arranged by size:
2109 1697 764 671 226 152 19
AseI AT'TA_AT
Cuts at: 160 1032 4192 160
Size: 872 3160 1606
Fragments arranged by size:
3160 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5638
AvaI C'yCGr_G
Cuts at: 1870 1943 2963 1870
Size: 73 1020 4545
Fragments arranged by size:
4545 1020 73
BamHI G'GATC_C
Cuts at: 2968 3335 2968
Size: 367 5271
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2959 4414 618
Size: 325 217 627 1172 1455 1842
Fragments arranged by size:
1842 1455 1172 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2957 1391
Size: 1566 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4667
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3829 3863 1903
Size: 34 1892 34 3678
Fragments arranged by size:
3678 1892 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3530 4740 3530
Size: 1210 4428
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5638
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2360 4249 136
Size: 107 122 71 631 605 688 1889 1525
Fragments arranged by size:
1889 1525 688 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5633 5633
Size: 5638
BlpI GC'TnA_GC
Cuts at: 3055 3055
Size: 5638
Bme1580I G_kGCm'C
Cuts at: 1790 3699 4855 1790
Size: 1909 1156 2573
Fragments arranged by size:
2573 1909 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5638
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3369 4327 442
Size: 416 53 83 399 24 1952 958 1753
Fragments arranged by size:
1952 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5638
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2572 4298 1239
Size: 1333 1726 2579
Fragments arranged by size:
2579 1726 1333
Bpu10I CC'TnA_GC
Cuts at: 2070 2617 2070
Size: 547 5091
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3763 4628 4926 3763
Size: 865 298 4475
Fragments arranged by size:
4475 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2606 4301 882
Size: 617 1107 1695 2219
Fragments arranged by size:
2219 1695 1107 617
BsaAI yAC'GTr
Cuts at: 493 1204 3429 493
Size: 711 2225 2702
Fragments arranged by size:
2702 2225 711
BsaBI GATnn'nnATC
Cuts at: 5632 5632
Size: 5638
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3445 3827 4462 275
Size: 53 83 186 936 1912 382 635 1451
Fragments arranged by size:
1912 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4071 4469 4743 4890 5301 4071
Size: 398 274 147 411 4408
Fragments arranged by size:
4408 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2570 2600 1178
Size: 30 165 30 1167 30 4216
Fragments arranged by size:
4216 1167 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5638
BseYI C'CCAG_C
Cuts at: 2550 4841 5337 2550
Size: 2291 496 2851
Fragments arranged by size:
2851 2291 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5065
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2371 3088 3849 4877 1540
Size: 275 186 370 717 761 1028 2301
Fragments arranged by size:
2301 1028 761 717 370 275 186
BsiHKAI G_wGCw'C
Cuts at: 2139 2685 3699 3784 4855 2139
Size: 546 1014 85 1071 2922
Fragments arranged by size:
2922 1071 1014 546 85
BsmI GAATG_Cn'
Cuts at: 3149 3242 3149
Size: 93 5545
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2139 2685 2957 3699 3784 4855 1391
Size: 399 349 546 272 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 546 399 349 272 85
BspEI T'CCGG_A
Cuts at: 5301 5301
Size: 5638
BspHI T'CATG_A
Cuts at: 3527 5257 3527
Size: 1730 3908
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2355 2670 2993 844
Size: 1511 315 323 3489
Fragments arranged by size:
3489 1511 323 315
BsrBI CCG'CTC
Cuts at: 1394 1823 3525 1394
Size: 429 1702 3507
Fragments arranged by size:
3507 1702 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2425 4133 4307 65
Size: 1183 1177 1708 174 1396
Fragments arranged by size:
1708 1396 1183 1177 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2370 4282 4469 1700
Size: 110 560 1912 187 2869
Fragments arranged by size:
2869 1912 560 187 110
BsrGI T'GTAC_A
Cuts at: 96 2838 96
Size: 2742 2896
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5638
BssSI C'ACGA_G
Cuts at: 3698 4710 3698
Size: 1012 4626
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5638
BstBI TT'CG_AA
Cuts at: 4442 4442
Size: 5638
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5638
BstXI CCAn_nnnn'nTGG
Cuts at: 2596 2596
Size: 5638
BstYI r'GATC_y
Cuts at: 1972 2968 3335 3720 3737 5189 5275 5287 5633
Size: 996 367 385 17 1452 86 12 346
Cuts at: 5633 1972
Size: 1977
Fragments arranged by size:
1977 1452 996 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2035 2882 2035
Size: 847 4791
BtgI C'CryG_G
Cuts at: 513 1065 2004 2420 513
Size: 552 939 416 3731
Fragments arranged by size:
3731 939 552 416
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4899
Fragments arranged by size:
4899 683 56
BtsI GCAGTG_nn'
Cuts at: 2118 3154 3948 3968 2118
Size: 1036 794 20 3788
Fragments arranged by size:
3788 1036 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5638
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5603
DraI TTT'AAA
Cuts at: 2942 2942
Size: 5638
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4645 809
Size: 507 3329 1802
Fragments arranged by size:
3329 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2148 2361 2379
Size: 54 1425 50 275 18 318 213 18
Cuts at: 2379 3085 3974 5603 8
Size: 706 889 1629 43
Fragments arranged by size:
1629 1425 889 706 318 275 213 54
50 43 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 3085 1537
Size: 275 1273 4090
Fragments arranged by size:
4090 1273 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2065 3568 1353
Size: 712 1503 3423
Fragments arranged by size:
3423 1503 712
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4225 4599 4745 387
Size: 1064 138 2636 374 146 1280
Fragments arranged by size:
2636 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2572 3701 4298 5085 5353 1239
Size: 393 940 1129 597 787 268 1524
Fragments arranged by size:
1524 1129 940 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2947 3325 2947
Size: 378 5260
EcoRV GAT'ATC
Cuts at: 2218 2218
Size: 5638
FspI TGC'GCA
Cuts at: 4144 4144
Size: 5638
HaeII r_GCGC'y
Cuts at: 1678 4785 1678
Size: 3107 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2570 2602 4279 4311 4353
Size: 32 135 32 998 32 1677 32 42
Cuts at: 4353 4385 1373
Size: 32 2626
Fragments arranged by size:
2626 1677 998 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2982 3228 669
Size: 988 281 1044 246 3079
Fragments arranged by size:
3079 1044 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5638
HpaI GTT'AAC
Cuts at: 3228 3228
Size: 5638
KpnI G_GTAC'C
Cuts at: 2963 2963
Size: 5638
MfeI C'AATT_G
Cuts at: 3237 3237
Size: 5638
MluI A'CGCG_T
Cuts at: 5514 5514
Size: 5638
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2088 4752 4936 1305
Size: 783 2664 184 2007
Fragments arranged by size:
2664 2007 783 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2150 2363 2381 10
Size: 54 1425 343 318 213 18 3267
Fragments arranged by size:
3267 1425 343 318 213 54 18
MslI CAynn'nnrTG
Cuts at: 518 1166 2123 2180 2403 2747 2834 2846 3596
Size: 648 957 57 223 344 87 12 750
Cuts at: 3596 3955 4114 518
Size: 359 159 2042
Fragments arranged by size:
2042 957 750 648 359 344 223 159
87 57 12
MspA1I CmG'CkG
Cuts at: 1606 3054 3731 4879 5124 1606
Size: 1448 677 1148 245 2120
Fragments arranged by size:
2120 1448 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2420 513
Size: 552 939 416 3731
Fragments arranged by size:
3731 939 552 416
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5638
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5638
NotI GC'GGCC_GC
Cuts at: 3085 3085
Size: 5638
NspI r_CATG'y
Cuts at: 1132 1229 2996 4541 1132
Size: 97 1767 1545 2229
Fragments arranged by size:
2229 1767 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5638
PciI A'CATG_T
Cuts at: 4537 4537
Size: 5638
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2599 2709 1108
Size: 1491 110 4037
Fragments arranged by size:
4037 1491 110
PmeI GTTT'AAAC
Cuts at: 2942 2942
Size: 5638
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3707 3739 5220 5252 3707
Size: 32 1481 32 4093
Fragments arranged by size:
4093 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5638
PsiI TTA'TAA
Cuts at: 3208 3208
Size: 5638
PstI C_TGCA'G
Cuts at: 830 2079 2701 2990 830
Size: 1249 622 289 3478
Fragments arranged by size:
3478 1249 622 289
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5638
SalI G'TCGA_C
Cuts at: 1936 2980 1936
Size: 1044 4594
SbfI CC_TGCA'GG
Cuts at: 2990 2990
Size: 5638
ScaI AGT'ACT
Cuts at: 3886 5598 3886
Size: 1712 3926
SfcI C'TryA_G
Cuts at: 826 1045 2075 2697 2745 2875 2986 4121 4802
Size: 219 1030 622 48 130 111 1135 681
Cuts at: 4802 4993 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1030 681 622 219 191 130
111 48
SfiI GGCCn_nnn'nGGCC
Cuts at: 2360 2360
Size: 5638
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5638
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5638
SmaI CCC'GGG
Cuts at: 1872 2965 1872
Size: 1093 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3742 4643 4905 820
Size: 197 926 1799 901 262 1553
Fragments arranged by size:
1799 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3429 493
Size: 2936 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5638
SphI G_CATG'C
Cuts at: 1132 2996 1132
Size: 1864 3774
SspI AAT'ATT
Cuts at: 5 52 3562 5
Size: 47 3510 2081
Fragments arranged by size:
3510 2081 47
StuI AGG'CCT
Cuts at: 1517 2717 2791 1517
Size: 1200 74 4364
Fragments arranged by size:
4364 1200 74
StyI C'CwwG_G
Cuts at: 513 1065 2004 2420 3077 513
Size: 552 939 416 657 3074
Fragments arranged by size:
3074 939 657 552 416
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2564 2869 3665 3682 3835 4020 4465
Size: 1255 984 305 796 17 153 185 445
Cuts at: 4465 325
Size: 1498
Fragments arranged by size:
1498 1255 984 796 445 305 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2184 2310 2802
Size: 274 80 33 51 1443 207 126 492
Cuts at: 2802 2838 3884 5596 96
Size: 36 1046 1712 138
Fragments arranged by size:
1712 1443 1046 492 274 207 138 126
80 51 36 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2456 2488 5598 5630 2456
Size: 32 3110 32 2464
Fragments arranged by size:
3110 2464 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5638
XbaI T'CTAG_A
Cuts at: 2974 2974
Size: 5638
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2371 2596 2371
Size: 225 5413
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5638
XmaI C'CCGG_G
Cuts at: 1870 2963 1870
Size: 1093 4545
XmnI GAAnn'nnTTC
Cuts at: 2717 2867 3767 2717
Size: 150 900 4588
Fragments arranged by size:
4588 900 150
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3446 276
Size: 53 83 186 936 1912 2468
Fragments arranged by size:
2468 1912 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI
SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI
EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfoI SrfI SwaI