Restriction map for
pFN21AE5919
(Circular) (Six-base) MAPSORT of: pFN21AE5919.seq Check: 9337 from: 1 to: 5635
pFN21AE5919 5635 bp
With 182 enzymes: SgfI *
December 8, 2014 13:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2729 2729
Size: 5635
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3445 278
Size: 53 83 186 936 1909 2468
Fragments arranged by size:
2468 1909 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2208 2978 1937
Size: 271 770 4594
Fragments arranged by size:
4594 770 271
Acc65I G'GTAC_C
Cuts at: 2956 2956
Size: 5635
AclI AA'CG_TT
Cuts at: 1563 3762 4135 1563
Size: 2199 373 3063
Fragments arranged by size:
3063 2199 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2663 3698 5082 5350 1632
Size: 1031 1035 1384 268 1917
Fragments arranged by size:
1917 1384 1035 1031 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5438
AflIII A'CryG_T
Cuts at: 1203 4534 5511 1203
Size: 3331 977 1327
Fragments arranged by size:
3331 1327 977
AgeI A'CCGG_T
Cuts at: 4466 4466
Size: 5635
AhdI GACnn_n'nnGTC
Cuts at: 4364 4364
Size: 5635
AleI CACnn'nnGTG
Cuts at: 1166 2736 1166
Size: 1570 4065
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2249 1184
Size: 1065 4570
AlwNI CAG_nnn'CTG
Cuts at: 3378 4950 3378
Size: 1572 4063
ApaLI G'TGCA_C
Cuts at: 3692 4848 3692
Size: 1156 4479
ApoI r'AATT_y
Cuts at: 1493 2520 2944 3170 3322 5431 1493
Size: 1027 424 226 152 2109 1697
Fragments arranged by size:
2109 1697 1027 424 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4189 160
Size: 872 3157 1606
Fragments arranged by size:
3157 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5635
AvaI C'yCGr_G
Cuts at: 1870 1943 2960 1870
Size: 73 1017 4545
Fragments arranged by size:
4545 1017 73
BamHI G'GATC_C
Cuts at: 2965 3332 2965
Size: 367 5268
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2233 2956 4411 618
Size: 325 217 627 446 723 1455 1842
Fragments arranged by size:
1842 1455 723 627 446 325 217
BanII G_rGCy'C
Cuts at: 1391 2091 2145 2954 1391
Size: 700 54 809 4072
Fragments arranged by size:
4072 809 700 54
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2101 928
Size: 971 202 4462
Fragments arranged by size:
4462 971 202
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2413 2447 3826 3860 1903
Size: 34 476 34 1379 34 3678
Fragments arranged by size:
3678 1379 476 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3527 4737 3527
Size: 1210 4425
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5635
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4246 136
Size: 107 122 71 631 605 2574 1525
Fragments arranged by size:
2574 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5630 5630
Size: 5635
BlpI GC'TnA_GC
Cuts at: 3052 3052
Size: 5635
Bme1580I G_kGCm'C
Cuts at: 1790 2238 3696 4852 1790
Size: 448 1458 1156 2573
Fragments arranged by size:
2573 1458 1156 448
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5635
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2054 2147 3366
Size: 416 53 83 399 24 637 93 1219
Cuts at: 3366 4324 442
Size: 958 1753
Fragments arranged by size:
1753 1219 958 637 416 399 93 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5635
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2143 2434 2475 4295 1239
Size: 904 291 41 1820 2579
Fragments arranged by size:
2579 1820 904 291 41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3760 4625 4923 3760
Size: 865 298 4472
Fragments arranged by size:
4472 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4298 882
Size: 617 2799 2219
Fragments arranged by size:
2799 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2371 3426 493
Size: 711 1167 1055 2702
Fragments arranged by size:
2702 1167 1055 711
BsaBI GATnn'nnATC
Cuts at: 5629 5629
Size: 5635
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3442 3824 4459 275
Size: 53 83 186 936 1909 382 635 1451
Fragments arranged by size:
1909 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4068 4466 4740 4887 5298 4068
Size: 398 274 147 411 4405
Fragments arranged by size:
4405 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5410
Fragments arranged by size:
5410 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2069 2330 1186
Size: 883 261 4491
Fragments arranged by size:
4491 883 261
BseYI C'CCAG_C
Cuts at: 2119 2914 4838 5334 2119
Size: 795 1924 496 2420
Fragments arranged by size:
2420 1924 795 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2150 2589 2701 1163
Size: 573 414 439 112 4097
Fragments arranged by size:
4097 573 439 414 112
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2205 3085 3846 4874 1540
Size: 275 186 204 880 761 1028 2301
Fragments arranged by size:
2301 1028 880 761 275 204 186
BsiHKAI G_wGCw'C
Cuts at: 3696 3781 4852 3696
Size: 85 1071 4479
Fragments arranged by size:
4479 1071 85
BsmI GAATG_Cn'
Cuts at: 3146 3239 3146
Size: 93 5542
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2091 2145 2238 2954 3696 3781 4852
Size: 399 301 54 93 716 742 85 1071
Cuts at: 4852 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 716 399 301 93 85
54
BspEI T'CCGG_A
Cuts at: 5298 5298
Size: 5635
BspHI T'CATG_A
Cuts at: 2406 3524 5254 2406
Size: 1118 1730 2787
Fragments arranged by size:
2787 1730 1118
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2729 2990 844
Size: 1885 261 3489
Fragments arranged by size:
3489 1885 261
BsrBI CCG'CTC
Cuts at: 1394 1823 3522 1394
Size: 429 1699 3507
Fragments arranged by size:
3507 1699 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4130 4304 65
Size: 1183 2882 174 1396
Fragments arranged by size:
2882 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4279 4466 1700
Size: 110 2469 187 2869
Fragments arranged by size:
2869 2469 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5635
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5635
BssSI C'ACGA_G
Cuts at: 3695 4707 3695
Size: 1012 4623
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5635
BstBI TT'CG_AA
Cuts at: 4439 4439
Size: 5635
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5635
BstYI r'GATC_y
Cuts at: 1972 2682 2965 3332 3717 3734 5186 5272 5284
Size: 710 283 367 385 17 1452 86 12
Cuts at: 5284 5630 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 710 385 367 346 283 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4144
Fragments arranged by size:
4144 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2197 487
Size: 683 56 971 3925
Fragments arranged by size:
3925 971 683 56
BtsI GCAGTG_nn'
Cuts at: 3151 3945 3965 3151
Size: 794 20 4821
Fragments arranged by size:
4821 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5635
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5600
DraI TTT'AAA
Cuts at: 2939 2939
Size: 5635
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4642 809
Size: 507 3326 1802
Fragments arranged by size:
3326 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2373 2400 2601
Size: 54 1425 50 275 18 543 27 201
Cuts at: 2601 3082 3971 5600 8
Size: 481 889 1629 43
Fragments arranged by size:
1629 1425 889 543 481 275 201 54
50 43 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 3082 1537
Size: 275 1270 4090
Fragments arranged by size:
4090 1270 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2096 2900 3565 1353
Size: 743 804 665 3423
Fragments arranged by size:
3423 804 743 665
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4222 4596 4742 387
Size: 1064 138 2633 374 146 1280
Fragments arranged by size:
2633 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2143 2434 2475 2663 3698 4295 5082
Size: 393 511 291 41 188 1035 597 787
Cuts at: 5082 5350 1239
Size: 268 1524
Fragments arranged by size:
1524 1035 787 597 511 393 291 268
188 41
EcoNI CCTnn'n_nnAGG
Cuts at: 2506 2506
Size: 5635
EcoO109I rG'GnC_Cy
Cuts at: 2511 2627 2511
Size: 116 5519
EcoRI G'AATT_C
Cuts at: 2944 3322 2944
Size: 378 5257
FspI TGC'GCA
Cuts at: 4141 4141
Size: 5635
HaeII r_GCGC'y
Cuts at: 1678 4782 1678
Size: 3104 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2409 2441 4276 4308 4350
Size: 32 135 32 837 32 1835 32 42
Cuts at: 4350 4382 1373
Size: 32 2626
Fragments arranged by size:
2626 1835 837 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2209 2979 3225 669
Size: 988 281 271 770 246 3079
Fragments arranged by size:
3079 988 770 281 271 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5635
HpaI GTT'AAC
Cuts at: 3225 3225
Size: 5635
KpnI G_GTAC'C
Cuts at: 2960 2960
Size: 5635
MfeI C'AATT_G
Cuts at: 2640 3234 2640
Size: 594 5041
MluI A'CGCG_T
Cuts at: 5511 5511
Size: 5635
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2761 4749 4933 1305
Size: 1456 1988 184 2007
Fragments arranged by size:
2007 1988 1456 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2375 2402 10
Size: 54 1425 343 543 27 3243
Fragments arranged by size:
3243 1425 543 343 54 27
MslI CAynn'nnrTG
Cuts at: 518 1166 2583 2656 2736 2771 3593 3952 4111
Size: 648 1417 73 80 35 822 359 159
Cuts at: 4111 518
Size: 2042
Fragments arranged by size:
2042 1417 822 648 359 159 80 73
35
MspA1I CmG'CkG
Cuts at: 1606 3051 3728 4876 5121 1606
Size: 1445 677 1148 245 2120
Fragments arranged by size:
2120 1445 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4144
Fragments arranged by size:
4144 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5635
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5635
NotI GC'GGCC_GC
Cuts at: 3082 3082
Size: 5635
NspI r_CATG'y
Cuts at: 1132 1229 2993 4538 1132
Size: 97 1764 1545 2229
Fragments arranged by size:
2229 1764 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5635
PciI A'CATG_T
Cuts at: 4534 4534
Size: 5635
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5635
PfoI T'CCnGG_A
Cuts at: 2444 2444
Size: 5635
PmeI GTTT'AAAC
Cuts at: 2939 2939
Size: 5635
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3704 3736 5217 5249 3704
Size: 32 1481 32 4090
Fragments arranged by size:
4090 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2627 2627
Size: 5635
PshAI GACnn'nnGTC
Cuts at: 1613 2632 1613
Size: 1019 4616
PsiI TTA'TAA
Cuts at: 3205 3205
Size: 5635
PstI C_TGCA'G
Cuts at: 830 2987 830
Size: 2157 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5635
SalI G'TCGA_C
Cuts at: 1936 2207 2977 1936
Size: 271 770 4594
Fragments arranged by size:
4594 770 271
SanDI GG'GwC_CC
Cuts at: 2627 2627
Size: 5635
SapI GCTCTTCn'nnn_
Cuts at: 2096 2096
Size: 5635
SbfI CC_TGCA'GG
Cuts at: 2987 2987
Size: 5635
ScaI AGT'ACT
Cuts at: 3883 5595 3883
Size: 1712 3923
SfcI C'TryA_G
Cuts at: 826 1045 2506 2869 2983 4118 4799 4990 826
Size: 219 1461 363 114 1135 681 191 1471
Fragments arranged by size:
1471 1461 1135 681 363 219 191 114
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5635
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5635
SmaI CCC'GGG
Cuts at: 1872 2962 1872
Size: 1090 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3739 4640 4902 820
Size: 197 926 1796 901 262 1553
Fragments arranged by size:
1796 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3426 493
Size: 2933 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5635
SphI G_CATG'C
Cuts at: 1132 2993 1132
Size: 1861 3774
SspI AAT'ATT
Cuts at: 5 52 3559 5
Size: 47 3507 2081
Fragments arranged by size:
3507 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5635
StyI C'CwwG_G
Cuts at: 513 1065 2004 2063 2802 3074 513
Size: 552 939 59 739 272 3074
Fragments arranged by size:
3074 939 739 552 272 59
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3662 3679 3832 4017 4462 325
Size: 1255 2082 17 153 185 445 1498
Fragments arranged by size:
2082 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2178 3881 5593
Size: 274 80 33 51 1443 201 1703 1712
Cuts at: 5593 96
Size: 138
Fragments arranged by size:
1712 1703 1443 274 201 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5595 5627 5595
Size: 32 5603
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5635
XbaI T'CTAG_A
Cuts at: 2971 2971
Size: 5635
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5635
XmaI C'CCGG_G
Cuts at: 1870 2960 1870
Size: 1090 4545
XmnI GAAnn'nnTTC
Cuts at: 2711 3764 2711
Size: 1053 4582
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3443 276
Size: 53 83 186 936 1909 2468
Fragments arranged by size:
2468 1909 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SexAI SfiI SfoI
SrfI SwaI XcmI