Restriction map for
pFN21AE5916
(Circular) (Six-base) MAPSORT of: pFN21AE5916.seq Check: 9560 from: 1 to: 5635
pFN21AE5916 5635 bp
With 182 enzymes: SgfI *
December 8, 2014 13:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3445 278
Size: 53 83 186 936 1909 2468
Fragments arranged by size:
2468 1909 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2978 1937
Size: 1041 4594
Acc65I G'GTAC_C
Cuts at: 2956 2956
Size: 5635
AclI AA'CG_TT
Cuts at: 1563 3762 4135 1563
Size: 2199 373 3063
Fragments arranged by size:
3063 2199 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2282 3698 5082 5350 1632
Size: 650 1416 1384 268 1917
Fragments arranged by size:
1917 1416 1384 650 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5438
AflIII A'CryG_T
Cuts at: 1203 2211 4534 5511 1203
Size: 1008 2323 977 1327
Fragments arranged by size:
2323 1327 1008 977
AgeI A'CCGG_T
Cuts at: 4466 4466
Size: 5635
AhdI GACnn_n'nnGTC
Cuts at: 4364 4364
Size: 5635
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5635
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5635
AlwNI CAG_nnn'CTG
Cuts at: 3378 4950 3378
Size: 1572 4063
ApaLI G'TGCA_C
Cuts at: 2422 3692 4848 2422
Size: 1270 1156 3209
Fragments arranged by size:
3209 1270 1156
ApoI r'AATT_y
Cuts at: 1493 2944 3170 3322 5431 1493
Size: 1451 226 152 2109 1697
Fragments arranged by size:
2109 1697 1451 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4189 160
Size: 872 3157 1606
Fragments arranged by size:
3157 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5635
AvaI C'yCGr_G
Cuts at: 1870 1943 2224 2960 1870
Size: 73 281 736 4545
Fragments arranged by size:
4545 736 281 73
BamHI G'GATC_C
Cuts at: 2682 2965 3332 2682
Size: 283 367 4985
Fragments arranged by size:
4985 367 283
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2956 4411 618
Size: 325 217 627 1169 1455 1842
Fragments arranged by size:
1842 1455 1169 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2145 2902 2954 1391
Size: 754 757 52 4072
Fragments arranged by size:
4072 757 754 52
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2176 928
Size: 971 277 4387
Fragments arranged by size:
4387 971 277
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3826 3860 1903
Size: 34 1889 34 3678
Fragments arranged by size:
3678 1889 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3527 4737 3527
Size: 1210 4425
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5635
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4246 136
Size: 107 122 71 631 605 2574 1525
Fragments arranged by size:
2574 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5630 5630
Size: 5635
BlpI GC'TnA_GC
Cuts at: 3052 3052
Size: 5635
Bme1580I G_kGCm'C
Cuts at: 1790 2426 3696 4852 1790
Size: 636 1270 1156 2573
Fragments arranged by size:
2573 1270 1156 636
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5635
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3366 4324 442
Size: 416 53 83 399 24 1949 958 1753
Fragments arranged by size:
1949 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2249 1056
Size: 1193 4442
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2090 2434 4295 1239
Size: 851 344 1861 2579
Fragments arranged by size:
2579 1861 851 344
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2698 2787 3760 4625 4923 2698
Size: 89 973 865 298 3410
Fragments arranged by size:
3410 973 865 298 89
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2653 4298 882
Size: 617 1154 1645 2219
Fragments arranged by size:
2219 1645 1154 617
BsaAI yAC'GTr
Cuts at: 493 1204 3426 493
Size: 711 2222 2702
Fragments arranged by size:
2702 2222 711
BsaBI GATnn'nnATC
Cuts at: 5629 5629
Size: 5635
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3442 3824 4459 275
Size: 53 83 186 936 1909 382 635 1451
Fragments arranged by size:
1909 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4068 4466 4740 4887 5298 4068
Size: 398 274 147 411 4405
Fragments arranged by size:
4405 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5410
Fragments arranged by size:
5410 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2716 1186
Size: 1530 4105
BseYI C'CCAG_C
Cuts at: 2062 4838 5334 2062
Size: 2776 496 2363
Fragments arranged by size:
2776 2363 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2150 1163
Size: 573 414 4648
Fragments arranged by size:
4648 573 414
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3085 3846 4874 1540
Size: 275 186 1084 761 1028 2301
Fragments arranged by size:
2301 1084 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2426 3696 3781 4852 2426
Size: 1270 85 1071 3209
Fragments arranged by size:
3209 1270 1071 85
BsmI GAATG_Cn'
Cuts at: 2887 3146 3239 2887
Size: 259 93 5283
Fragments arranged by size:
5283 259 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2145 2426 2902 2954 3696 3781 4852
Size: 399 355 281 476 52 742 85 1071
Cuts at: 4852 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 476 399 355 281 85
52
BspEI T'CCGG_A
Cuts at: 5298 5298
Size: 5635
BspHI T'CATG_A
Cuts at: 3524 5254 3524
Size: 1730 3905
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2729 2990 844
Size: 1885 261 3489
Fragments arranged by size:
3489 1885 261
BsrBI CCG'CTC
Cuts at: 1394 1823 2632 3522 1394
Size: 429 809 890 3507
Fragments arranged by size:
3507 890 809 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4130 4304 65
Size: 1183 2882 174 1396
Fragments arranged by size:
2882 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4279 4466 1700
Size: 110 2469 187 2869
Fragments arranged by size:
2869 2469 187 110
BsrGI T'GTAC_A
Cuts at: 96 2775 96
Size: 2679 2956
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5635
BssSI C'ACGA_G
Cuts at: 3695 4707 3695
Size: 1012 4623
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5635
BstBI TT'CG_AA
Cuts at: 4439 4439
Size: 5635
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5635
BstYI r'GATC_y
Cuts at: 1972 2682 2817 2965 3332 3717 3734 5186 5272
Size: 710 135 148 367 385 17 1452 86
Cuts at: 5272 5284 5630 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 710 385 367 346 148 135
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2876 2876
Size: 5635
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4144
Fragments arranged by size:
4144 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4896
Fragments arranged by size:
4896 683 56
BtsI GCAGTG_nn'
Cuts at: 3151 3945 3965 3151
Size: 794 20 4821
Fragments arranged by size:
4821 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5635
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5600
DraI TTT'AAA
Cuts at: 2939 2939
Size: 5635
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4642 809
Size: 507 3326 1802
Fragments arranged by size:
3326 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2199 2670 3082
Size: 54 1425 50 275 18 369 471 412
Cuts at: 3082 3971 5600 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 471 412 369 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3082 1537
Size: 275 1270 4090
Fragments arranged by size:
4090 1270 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3565 1353
Size: 2212 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4222 4596 4742 387
Size: 1064 138 2633 374 146 1280
Fragments arranged by size:
2633 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2090 2282 2434 3698 4295 5082 5350
Size: 393 458 192 152 1264 597 787 268
Cuts at: 5350 1239
Size: 1524
Fragments arranged by size:
1524 1264 787 597 458 393 268 192
152
EcoRI G'AATT_C
Cuts at: 2944 3322 2944
Size: 378 5257
FspI TGC'GCA
Cuts at: 4141 4141
Size: 5635
HaeII r_GCGC'y
Cuts at: 1678 2766 4782 1678
Size: 1088 2016 2531
Fragments arranged by size:
2531 2016 1088
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4276 4308 4350 4382 1373
Size: 32 135 32 2704 32 42 32 2626
Fragments arranged by size:
2704 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2979 3225 669
Size: 988 281 1041 246 3079
Fragments arranged by size:
3079 1041 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5635
HpaI GTT'AAC
Cuts at: 3225 3225
Size: 5635
KpnI G_GTAC'C
Cuts at: 2960 2960
Size: 5635
MfeI C'AATT_G
Cuts at: 3234 3234
Size: 5635
MluI A'CGCG_T
Cuts at: 5511 5511
Size: 5635
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4749 4933 1305
Size: 3444 184 2007
Fragments arranged by size:
3444 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2201 2672 10
Size: 54 1425 343 369 471 2973
Fragments arranged by size:
2973 1425 471 369 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2174 2621 2666 2771 2777 3593 3952
Size: 648 1008 447 45 105 6 816 359
Cuts at: 3952 4111 518
Size: 159 2042
Fragments arranged by size:
2042 1008 816 648 447 359 159 105
45 6
MspA1I CmG'CkG
Cuts at: 1606 3051 3728 4876 5121 1606
Size: 1445 677 1148 245 2120
Fragments arranged by size:
2120 1445 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4144
Fragments arranged by size:
4144 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5635
NheI G'CTAG_C
Cuts at: 1052 2245 1052
Size: 1193 4442
NotI GC'GGCC_GC
Cuts at: 3082 3082
Size: 5635
NspI r_CATG'y
Cuts at: 1132 1229 2215 2993 4538 1132
Size: 97 986 778 1545 2229
Fragments arranged by size:
2229 1545 986 778 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5635
PciI A'CATG_T
Cuts at: 2211 4534 2211
Size: 2323 3312
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5635
PmeI GTTT'AAAC
Cuts at: 2939 2939
Size: 5635
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3704 3736 5217 5249 3704
Size: 32 1481 32 4090
Fragments arranged by size:
4090 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5635
PsiI TTA'TAA
Cuts at: 3205 3205
Size: 5635
PspXI vC'TCGA_Gb
Cuts at: 2224 2224
Size: 5635
PstI C_TGCA'G
Cuts at: 830 2987 830
Size: 2157 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5635
SalI G'TCGA_C
Cuts at: 1936 2977 1936
Size: 1041 4594
SbfI CC_TGCA'GG
Cuts at: 2987 2987
Size: 5635
ScaI AGT'ACT
Cuts at: 2230 3883 5595 2230
Size: 1653 1712 2270
Fragments arranged by size:
2270 1712 1653
SexAI A'CCwGG_T
Cuts at: 2586 2586
Size: 5635
SfcI C'TryA_G
Cuts at: 826 1045 2104 2869 2983 4118 4799 4990 826
Size: 219 1059 765 114 1135 681 191 1471
Fragments arranged by size:
1471 1135 1059 765 681 219 191 114
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5635
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5635
SmaI CCC'GGG
Cuts at: 1872 2962 1872
Size: 1090 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2224 2677 2802 3739 4640 4902
Size: 197 926 281 453 125 937 901 262
Cuts at: 4902 820
Size: 1553
Fragments arranged by size:
1553 937 926 901 453 281 262 197
125
SnaBI TAC'GTA
Cuts at: 493 3426 493
Size: 2933 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5635
SphI G_CATG'C
Cuts at: 1132 2993 1132
Size: 1861 3774
SspI AAT'ATT
Cuts at: 5 52 3559 5
Size: 47 3507 2081
Fragments arranged by size:
3507 2081 47
StuI AGG'CCT
Cuts at: 1517 2711 1517
Size: 1194 4441
StyI C'CwwG_G
Cuts at: 513 1065 2004 3074 513
Size: 552 939 1070 3074
Fragments arranged by size:
3074 1070 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2188 3662 3679 3832 4017 4462 325
Size: 1255 608 1474 17 153 185 445 1498
Fragments arranged by size:
1498 1474 1255 608 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2228 2775 3881
Size: 274 80 33 51 1443 251 547 1106
Cuts at: 3881 5593 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1106 547 274 251 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2040 2072 5595 5627 2040
Size: 32 3523 32 2048
Fragments arranged by size:
3523 2048 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5635
XbaI T'CTAG_A
Cuts at: 2159 2971 2159
Size: 812 4823
XhoI C'TCGA_G
Cuts at: 1943 2224 1943
Size: 281 5354
XmaI C'CCGG_G
Cuts at: 1870 2960 1870
Size: 1090 4545
XmnI GAAnn'nnTTC
Cuts at: 3764 3764
Size: 5635
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3443 276
Size: 53 83 186 936 1909 2468
Fragments arranged by size:
2468 1909 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspXI PstI PvuI SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PsrI PvuII RsrII SacI SacII
SanDI SapI SfiI SfoI SrfI SwaI XcmI