Restriction map for
pFN21AE5915
(Circular) (Six-base) MAPSORT of: pFN21AE5915.seq Check: 4205 from: 1 to: 5632
pFN21AE5915 5632 bp
With 182 enzymes: SgfI *
December 8, 2014 13:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2726 2726
Size: 5632
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3442 278
Size: 53 83 186 936 1906 2468
Fragments arranged by size:
2468 1906 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2975 1937
Size: 1038 4594
Acc65I G'GTAC_C
Cuts at: 2953 2953
Size: 5632
AclI AA'CG_TT
Cuts at: 1563 3759 4132 1563
Size: 2196 373 3063
Fragments arranged by size:
3063 2196 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3695 5079 5347 1632
Size: 2063 1384 268 1917
Fragments arranged by size:
2063 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5435
AflIII A'CryG_T
Cuts at: 1203 4531 5508 1203
Size: 3328 977 1327
Fragments arranged by size:
3328 1327 977
AgeI A'CCGG_T
Cuts at: 4463 4463
Size: 5632
AhdI GACnn_n'nnGTC
Cuts at: 4361 4361
Size: 5632
AleI CACnn'nnGTG
Cuts at: 1166 2532 1166
Size: 1366 4266
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5632
AlwNI CAG_nnn'CTG
Cuts at: 3375 4947 3375
Size: 1572 4060
ApaLI G'TGCA_C
Cuts at: 2422 3689 4845 2422
Size: 1267 1156 3209
Fragments arranged by size:
3209 1267 1156
ApoI r'AATT_y
Cuts at: 1493 2941 3167 3319 5428 1493
Size: 1448 226 152 2109 1697
Fragments arranged by size:
2109 1697 1448 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4186 160
Size: 872 3154 1606
Fragments arranged by size:
3154 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5632
AvaI C'yCGr_G
Cuts at: 1870 1943 2957 1870
Size: 73 1014 4545
Fragments arranged by size:
4545 1014 73
BamHI G'GATC_C
Cuts at: 2679 2962 3329 2679
Size: 283 367 4982
Fragments arranged by size:
4982 367 283
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2894 2953 4408 618
Size: 325 217 627 1107 59 1455 1842
Fragments arranged by size:
1842 1455 1107 627 325 217 59
BanII G_rGCy'C
Cuts at: 1391 2951 1391
Size: 1560 4072
BbeI G_GCGC'C
Cuts at: 2898 2898
Size: 5632
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4661
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3823 3857 1903
Size: 34 1886 34 3678
Fragments arranged by size:
3678 1886 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3524 4734 3524
Size: 1210 4422
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5632
BfrBI ATG'CAT
Cuts at: 2292 2292
Size: 5632
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4243 136
Size: 107 122 71 631 605 2571 1525
Fragments arranged by size:
2571 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5627 5627
Size: 5632
BlpI GC'TnA_GC
Cuts at: 3049 3049
Size: 5632
Bme1580I G_kGCm'C
Cuts at: 1790 2426 2911 3693 4849 1790
Size: 636 485 782 1156 2573
Fragments arranged by size:
2573 1156 782 636 485
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5632
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3363 4321 442
Size: 416 53 83 399 24 1946 958 1753
Fragments arranged by size:
1946 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5632
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2090 2434 4292 1239
Size: 851 344 1858 2579
Fragments arranged by size:
2579 1858 851 344
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2537 2695 3757 4622 4920 2537
Size: 158 1062 865 298 3249
Fragments arranged by size:
3249 1062 865 298 158
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2650 4295 882
Size: 617 1151 1645 2219
Fragments arranged by size:
2219 1645 1151 617
BsaAI yAC'GTr
Cuts at: 493 1204 2329 2772 3423 493
Size: 711 1125 443 651 2702
Fragments arranged by size:
2702 1125 711 651 443
BsaBI GATnn'nnATC
Cuts at: 5626 5626
Size: 5632
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2895 3439 3821 4456
Size: 53 83 186 936 1362 544 382 635
Cuts at: 4456 275
Size: 1451
Fragments arranged by size:
1451 1362 936 635 544 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4065 4463 4737 4884 5295 4065
Size: 398 274 147 411 4402
Fragments arranged by size:
4402 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5407
Fragments arranged by size:
5407 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2069 2713 2816 2917 1186
Size: 883 644 103 101 3901
Fragments arranged by size:
3901 883 644 103 101
BseYI C'CCAG_C
Cuts at: 2119 4835 5331 2119
Size: 2716 496 2420
Fragments arranged by size:
2716 2420 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5059
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3082 3843 4871 1540
Size: 275 186 1081 761 1028 2301
Fragments arranged by size:
2301 1081 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2426 3693 3778 4849 2426
Size: 1267 85 1071 3209
Fragments arranged by size:
3209 1267 1071 85
BsmI GAATG_Cn'
Cuts at: 2884 3143 3236 2884
Size: 259 93 5280
Fragments arranged by size:
5280 259 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2426 2911 2951 3693 3778 4849 1391
Size: 399 636 485 40 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 636 485 399 85 40
BspEI T'CCGG_A
Cuts at: 5295 5295
Size: 5632
BspHI T'CATG_A
Cuts at: 2406 2515 3521 5251 2406
Size: 109 1006 1730 2787
Fragments arranged by size:
2787 1730 1006 109
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2726 2987 844
Size: 1882 261 3489
Fragments arranged by size:
3489 1882 261
BsrBI CCG'CTC
Cuts at: 1394 1823 3519 1394
Size: 429 1696 3507
Fragments arranged by size:
3507 1696 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4127 4301 65
Size: 1183 2879 174 1396
Fragments arranged by size:
2879 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4276 4463 1700
Size: 110 2466 187 2869
Fragments arranged by size:
2869 2466 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5632
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5632
BssSI C'ACGA_G
Cuts at: 3692 4704 3692
Size: 1012 4620
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5632
BstBI TT'CG_AA
Cuts at: 4436 4436
Size: 5632
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5632
BstYI r'GATC_y
Cuts at: 1972 2679 2962 3329 3714 3731 5183 5269 5281
Size: 707 283 367 385 17 1452 86 12
Cuts at: 5281 5627 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 707 385 367 346 283 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2492 2799 2873 2900 2492
Size: 307 74 27 5224
Fragments arranged by size:
5224 307 74 27
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4141
Fragments arranged by size:
4141 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4893
Fragments arranged by size:
4893 683 56
BtsI GCAGTG_nn'
Cuts at: 3148 3942 3962 3148
Size: 794 20 4818
Fragments arranged by size:
4818 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5632
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5597
DraI TTT'AAA
Cuts at: 2936 2936
Size: 5632
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4639 809
Size: 507 3323 1802
Fragments arranged by size:
3323 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2199 2664 3079
Size: 54 1425 50 275 18 369 465 415
Cuts at: 3079 3968 5597 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 465 415 369 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3079 1537
Size: 275 1267 4090
Fragments arranged by size:
4090 1267 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2753 3562 1353
Size: 1400 809 3423
Fragments arranged by size:
3423 1400 809
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4219 4593 4739 387
Size: 1064 138 2630 374 146 1280
Fragments arranged by size:
2630 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2090 2434 3695 4292 5079 5347 1239
Size: 393 458 344 1261 597 787 268 1524
Fragments arranged by size:
1524 1261 787 597 458 393 344 268
EcoRI G'AATT_C
Cuts at: 2941 3319 2941
Size: 378 5254
FspI TGC'GCA
Cuts at: 2657 4138 2657
Size: 1481 4151
HaeII r_GCGC'y
Cuts at: 1678 2763 2898 4779 1678
Size: 1085 135 1881 2531
Fragments arranged by size:
2531 1881 1085 135
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4273 4305 4347 4379 1373
Size: 32 135 32 2701 32 42 32 2626
Fragments arranged by size:
2701 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2209 2976 3222 669
Size: 988 281 271 767 246 3079
Fragments arranged by size:
3079 988 767 281 271 246
HindIII A'AGCT_T
Cuts at: 748 2162 748
Size: 1414 4218
HpaI GTT'AAC
Cuts at: 3222 3222
Size: 5632
KasI G'GCGC_C
Cuts at: 2894 2894
Size: 5632
KpnI G_GTAC'C
Cuts at: 2957 2957
Size: 5632
MfeI C'AATT_G
Cuts at: 3231 3231
Size: 5632
MluI A'CGCG_T
Cuts at: 5508 5508
Size: 5632
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2250 4746 4930 1305
Size: 945 2496 184 2007
Fragments arranged by size:
2496 2007 945 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2201 10
Size: 54 1425 343 369 3441
Fragments arranged by size:
3441 1425 369 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2174 2532 2618 2774 3590 3949 4108
Size: 648 1008 358 86 156 816 359 159
Cuts at: 4108 518
Size: 2042
Fragments arranged by size:
2042 1008 816 648 359 358 159 156
86
MspA1I CmG'CkG
Cuts at: 1606 3048 3725 4873 5118 1606
Size: 1442 677 1148 245 2120
Fragments arranged by size:
2120 1442 1148 677 245
NarI GG'CG_CC
Cuts at: 2895 2895
Size: 5632
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4141
Fragments arranged by size:
4141 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5632
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5632
NotI GC'GGCC_GC
Cuts at: 3079 3079
Size: 5632
NsiI A_TGCA'T
Cuts at: 2294 2294
Size: 5632
NspI r_CATG'y
Cuts at: 1132 1229 2990 4535 1132
Size: 97 1761 1545 2229
Fragments arranged by size:
2229 1761 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5632
PciI A'CATG_T
Cuts at: 4531 4531
Size: 5632
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5632
PmeI GTTT'AAAC
Cuts at: 2936 2936
Size: 5632
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3701 3733 5214 5246 3701
Size: 32 1481 32 4087
Fragments arranged by size:
4087 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5632
PsiI TTA'TAA
Cuts at: 3202 3202
Size: 5632
PstI C_TGCA'G
Cuts at: 830 2984 830
Size: 2154 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5632
SalI G'TCGA_C
Cuts at: 1936 2974 1936
Size: 1038 4594
SbfI CC_TGCA'GG
Cuts at: 2984 2984
Size: 5632
ScaI AGT'ACT
Cuts at: 3880 5592 3880
Size: 1712 3920
SexAI A'CCwGG_T
Cuts at: 2583 2583
Size: 5632
SfcI C'TryA_G
Cuts at: 826 1045 2980 4115 4796 4987 826
Size: 219 1935 1135 681 191 1471
Fragments arranged by size:
1935 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2896 2896
Size: 5632
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5632
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5632
SmaI CCC'GGG
Cuts at: 1872 2959 1872
Size: 1087 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2552 2674 3736 4637 4899 820
Size: 197 926 609 122 1062 901 262 1553
Fragments arranged by size:
1553 1062 926 901 609 262 197 122
SnaBI TAC'GTA
Cuts at: 493 3423 493
Size: 2930 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5632
SphI G_CATG'C
Cuts at: 1132 2990 1132
Size: 1858 3774
SspI AAT'ATT
Cuts at: 5 52 3556 5
Size: 47 3504 2081
Fragments arranged by size:
3504 2081 47
StuI AGG'CCT
Cuts at: 1517 2708 1517
Size: 1191 4441
StyI C'CwwG_G
Cuts at: 513 1065 2004 3071 513
Size: 552 939 1067 3074
Fragments arranged by size:
3074 1067 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2188 2437 3659 3676 3829 4014 4459
Size: 1255 608 249 1222 17 153 185 445
Cuts at: 4459 325
Size: 1498
Fragments arranged by size:
1498 1255 1222 608 445 249 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2178 3878 5590
Size: 274 80 33 51 1443 201 1700 1712
Cuts at: 5590 96
Size: 138
Fragments arranged by size:
1712 1700 1443 274 201 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5592 5624 5592
Size: 32 5600
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5632
XbaI T'CTAG_A
Cuts at: 2968 2968
Size: 5632
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5632
XmaI C'CCGG_G
Cuts at: 1870 2957 1870
Size: 1087 4545
XmnI GAAnn'nnTTC
Cuts at: 3761 3761
Size: 5632
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3440 276
Size: 53 83 186 936 1906 2468
Fragments arranged by size:
2468 1906 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI SalI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SfiI SrfI SwaI
XcmI