Restriction map for
pFN21AE5842
(Circular) (Six-base) MAPSORT of: pFN21AE5842.seq Check: 1890 from: 1 to: 10321
pFN21AE5842 10321 bp
With 182 enzymes: SgfI *
November 21, 2014 12:14 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 8131 278
Size: 53 83 186 936 6595 2468
Fragments arranged by size:
6595 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6770 7664 1937
Size: 4833 894 4594
Fragments arranged by size:
4833 4594 894
Acc65I G'GTAC_C
Cuts at: 7642 7642
Size: 10321
AclI AA'CG_TT
Cuts at: 1563 8448 8821 1563
Size: 6885 373 3063
Fragments arranged by size:
6885 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2533 2631 3093 3629 3957 4718 5265 5301
Size: 901 98 462 536 328 761 547 36
Cuts at: 5301 7182 7283 7438 8384 9768 10036 1632
Size: 1881 101 155 946 1384 268 1917
Fragments arranged by size:
1917 1881 1384 946 901 761 547 536
462 328 268 155 101 98 36
AfeI AGC'GCT
Cuts at: 7517 7517
Size: 10321
AflII C'TTAA_G
Cuts at: 820 1017 4595 5705 5732 820
Size: 197 3578 1110 27 5409
Fragments arranged by size:
5409 3578 1110 197 27
AflIII A'CryG_T
Cuts at: 1203 3669 5322 9220 10197 1203
Size: 2466 1653 3898 977 1327
Fragments arranged by size:
3898 2466 1653 1327 977
AgeI A'CCGG_T
Cuts at: 9152 9152
Size: 10321
AhdI GACnn_n'nnGTC
Cuts at: 9050 9050
Size: 10321
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 10321
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2486 2673 3381 4677 4859 1184
Size: 1302 187 708 1296 182 6646
Fragments arranged by size:
6646 1302 1296 708 187 182
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6072 6104 6072
Size: 32 10289
AlwNI CAG_nnn'CTG
Cuts at: 2573 2609 4283 8064 9636 2573
Size: 36 1674 3781 1572 3258
Fragments arranged by size:
3781 3258 1674 1572 36
ApaI G_GGCC'C
Cuts at: 3831 5133 6927 3831
Size: 1302 1794 7225
Fragments arranged by size:
7225 1794 1302
ApaLI G'TGCA_C
Cuts at: 3882 8378 9534 3882
Size: 4496 1156 4669
Fragments arranged by size:
4669 4496 1156
ApoI r'AATT_y
Cuts at: 1493 2699 2963 3155 3540 3621 5030 5141 5615
Size: 1206 264 192 385 81 1409 111 474
Cuts at: 5615 5627 5834 6608 7630 7856 8008 10117 1493
Size: 12 207 774 1022 226 152 2109 1697
Fragments arranged by size:
2109 1697 1409 1206 1022 774 474 385
264 226 207 192 152 111 81 12
AseI AT'TA_AT
Cuts at: 160 1032 8875 160
Size: 872 7843 1606
Fragments arranged by size:
7843 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10321
AvaI C'yCGr_G
Cuts at: 1870 1943 2491 6327 6981 7646 1870
Size: 73 548 3836 654 665 4545
Fragments arranged by size:
4545 3836 665 654 548 73
BamHI G'GATC_C
Cuts at: 7651 8018 7651
Size: 367 9954
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2279 3749 5807 6132 7642
Size: 325 217 627 492 1470 2058 325 1510
Cuts at: 7642 9097 618
Size: 1455 1842
Fragments arranged by size:
2058 1842 1510 1470 1455 627 492 325
325 217
BanII G_rGCy'C
Cuts at: 1391 3831 5133 6927 7640 1391
Size: 2440 1302 1794 713 4072
Fragments arranged by size:
4072 2440 1794 1302 713
BbeI G_GCGC'C
Cuts at: 6136 6136
Size: 10321
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2457 3734 4967 6465 928
Size: 971 558 1277 1233 1498 4784
Fragments arranged by size:
4784 1498 1277 1233 971 558
BbvCI CC'TCA_GC
Cuts at: 3288 6936 3288
Size: 3648 6673
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3833 3867 8512 8546 1903
Size: 34 1896 34 4645 34 3678
Fragments arranged by size:
4645 3678 1896 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4470 8213 9423 4470
Size: 3743 1210 5368
Fragments arranged by size:
5368 3743 1210
BclI T'GATC_A
Cuts at: 1548 2235 4293 1548
Size: 687 2058 7576
Fragments arranged by size:
7576 2058 687
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3306 8932 136
Size: 107 122 71 631 605 1634 5626 1525
Fragments arranged by size:
5626 1634 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6196 6408 10316 6196
Size: 212 3908 6201
Fragments arranged by size:
6201 3908 212
BlpI GC'TnA_GC
Cuts at: 5172 5275 5416 7738 5172
Size: 103 141 2322 7755
Fragments arranged by size:
7755 2322 141 103
Bme1580I G_kGCm'C
Cuts at: 1790 3831 3886 5133 6927 8382 9538 1790
Size: 2041 55 1247 1794 1455 1156 2573
Fragments arranged by size:
2573 2041 1794 1455 1247 1156 55
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 10321
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4866 6590 7029
Size: 416 53 83 399 24 3449 1724 439
Cuts at: 7029 8052 9010 442
Size: 1023 958 1753
Fragments arranged by size:
3449 1753 1724 1023 958 439 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10321
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3583 7534 3583
Size: 3951 6370
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2118 2180 2892 3000 4161 4719 5142 6210
Size: 879 62 712 108 1161 558 423 1068
Cuts at: 6210 6371 7029 8981 1239
Size: 161 658 1952 2579
Fragments arranged by size:
2579 1952 1161 1068 879 712 658 558
423 161 108 62
Bpu10I CC'TnA_GC
Cuts at: 3288 3468 6936 3288
Size: 180 3468 6673
Fragments arranged by size:
6673 3468 180
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3188 5483 6017 6866 7110 8446 9311 9609 3188
Size: 2295 534 849 244 1336 865 298 3900
Fragments arranged by size:
3900 2295 1336 865 849 534 298 244
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6650 8984 882
Size: 617 5151 2334 2219
Fragments arranged by size:
5151 2334 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5027 5360 6665 8112 493
Size: 711 3823 333 1305 1447 2702
Fragments arranged by size:
3823 2702 1447 1305 711 333
BsaBI GATnn'nnATC
Cuts at: 4032 7086 10315 4032
Size: 3054 3229 4038
Fragments arranged by size:
4038 3229 3054
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6133 8128 8510 9145
Size: 53 83 186 936 4600 1995 382 635
Cuts at: 9145 275
Size: 1451
Fragments arranged by size:
4600 1995 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4530 5426 8754 9152 9426 9573 9984 4530
Size: 896 3328 398 274 147 411 4867
Fragments arranged by size:
4867 3328 896 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2825 2855 3563 3593 5894
Size: 30 165 30 1422 30 708 30 2301
Cuts at: 5894 5924 6276 6306 1178
Size: 30 352 30 5193
Fragments arranged by size:
5193 2301 1422 708 352 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2466 5918 5921 7430 7499 7514 7517 7577
Size: 1280 3452 3 1509 69 15 3 60
Cuts at: 7577 1186
Size: 3930
Fragments arranged by size:
3930 3452 1509 1280 69 60 15 3
3
BseYI C'CCAG_C
Cuts at: 2224 2639 3061 3441 5070 7187 7592 9524 10020
Size: 415 422 380 1629 2117 405 1932 496
Cuts at: 10020 2224
Size: 2525
Fragments arranged by size:
2525 2117 1932 1629 496 422 415 405
380
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 9748
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7771 8532 9560 1540
Size: 275 186 5770 761 1028 2301
Fragments arranged by size:
5770 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2779 2846 3886 4147 4255 8382 8467 9538 2779
Size: 67 1040 261 108 4127 85 1071 3562
Fragments arranged by size:
4127 3562 1071 1040 261 108 85 67
BsmI GAATG_Cn'
Cuts at: 3340 3775 5403 7832 7925 3340
Size: 435 1628 2429 93 5736
Fragments arranged by size:
5736 2429 1628 435 93
BsmBI CGTCTCn'nnnn_
Cuts at: 7350 7350
Size: 10321
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2779 2846 3831 3886 4147 4255 5133
Size: 399 989 67 985 55 261 108 878
Cuts at: 5133 6927 7640 8382 8467 9538 1391
Size: 1794 713 742 85 1071 2174
Fragments arranged by size:
2174 1794 1071 989 985 878 742 713
399 261 108 85 67 55
BspEI T'CCGG_A
Cuts at: 4530 5426 9984 4530
Size: 896 4558 4867
Fragments arranged by size:
4867 4558 896
BspHI T'CATG_A
Cuts at: 8210 9940 8210
Size: 1730 8591
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4072 7045 7676 844
Size: 3228 2973 631 3489
Fragments arranged by size:
3489 3228 2973 631
BsrBI CCG'CTC
Cuts at: 1394 1823 5380 8208 1394
Size: 429 3557 2828 3507
Fragments arranged by size:
3557 3507 2828 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4818 7587 8816 8990 65
Size: 1183 3570 2769 1229 174 1396
Fragments arranged by size:
3570 2769 1396 1229 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6129 8965 9152 1700
Size: 110 4319 2836 187 2869
Fragments arranged by size:
4319 2869 2836 187 110
BsrGI T'GTAC_A
Cuts at: 96 7114 96
Size: 7018 3303
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10321
BssSI C'ACGA_G
Cuts at: 8381 9393 8381
Size: 1012 9309
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5807 794
Size: 5013 5308
BstBI TT'CG_AA
Cuts at: 5623 9125 5623
Size: 3502 6819
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 10321
BstXI CCAn_nnnn'nTGG
Cuts at: 2766 2978 3823 6579 2766
Size: 212 845 2756 6508
Fragments arranged by size:
6508 2756 845 212
BstYI r'GATC_y
Cuts at: 1972 2826 3424 3691 4135 4867 6196 6408 6427
Size: 854 598 267 444 732 1329 212 19
Cuts at: 6427 7262 7651 8018 8403 8420 9872 9958 9970
Size: 835 389 367 385 17 1452 86 12
Cuts at: 9970 10316 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1329 854 835 732 598 444
389 385 367 346 267 212 86 19
17 12
BstZ17I GTA'TAC
Cuts at: 6771 6771
Size: 10321
Bsu36I CC'TnA_GG
Cuts at: 2586 4008 5043 2586
Size: 1422 1035 7864
Fragments arranged by size:
7864 1422 1035
BtgI C'CryG_G
Cuts at: 513 1065 2004 3695 4469 4872 5149 7375 513
Size: 552 939 1691 774 403 277 2226 3459
Fragments arranged by size:
3459 2226 1691 939 774 552 403 277
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3353 3836 487
Size: 683 56 2127 483 6972
Fragments arranged by size:
6972 2127 683 483 56
BtsI GCAGTG_nn'
Cuts at: 2049 2921 3088 4364 4661 4788 7837 8631 8651
Size: 872 167 1276 297 127 3049 794 20
Cuts at: 8651 2049
Size: 3719
Fragments arranged by size:
3719 3049 1276 872 794 297 167 127
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10321
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 10286
DraI TTT'AAA
Cuts at: 7625 7625
Size: 10321
DraIII CAC_nnn'GTG
Cuts at: 4878 4878
Size: 10321
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6160 6545 6762 9328 809
Size: 507 4844 385 217 2566 1802
Fragments arranged by size:
4844 2566 1802 507 385 217
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4213 4351 4466
Size: 54 1425 50 275 18 2383 138 115
Cuts at: 4466 6272 6581 6732 7768 8657 10286 8
Size: 1806 309 151 1036 889 1629 43
Fragments arranged by size:
2383 1806 1629 1425 1036 889 309 275
151 138 115 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 7768 1537
Size: 275 5956 4090
Fragments arranged by size:
5956 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2814 3552 3608 4529 4620 5043 5482 5520
Size: 1461 738 56 921 91 423 439 38
Cuts at: 5520 5652 6327 7095 7503 8251 1353
Size: 132 675 768 408 748 3423
Fragments arranged by size:
3423 1461 921 768 748 738 675 439
423 408 132 91 56 38
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2785 5692 8908 9282 9428 387
Size: 1064 138 1196 2907 3216 374 146 1280
Fragments arranged by size:
3216 2907 1280 1196 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2118 2180 2533 2631 2892 3000 3093
Size: 393 486 62 353 98 261 108 93
Cuts at: 3093 3629 3957 4161 4718 4719 5142 5265 5301
Size: 536 328 204 557 1 423 123 36
Cuts at: 5301 6210 6371 7029 7182 7283 7438 8384 8981
Size: 909 161 658 153 101 155 946 597
Cuts at: 8981 9768 10036 1239
Size: 787 268 1524
Fragments arranged by size:
1524 946 909 787 658 597 557 536
486 423 393 353 328 268 261 204
161 155 153 123 108 101 98 93
62 36 1
EcoNI CCTnn'n_nnAGG
Cuts at: 7142 7553 7142
Size: 411 9910
EcoO109I rG'GnC_Cy
Cuts at: 2009 2582 3827 3899 4012 4812 5129 5738 6923
Size: 573 1245 72 113 800 317 609 1185
Cuts at: 6923 6924 2009
Size: 1 5406
Fragments arranged by size:
5406 1245 1185 800 609 573 317 113
72 1
EcoRI G'AATT_C
Cuts at: 3621 5615 7630 8008 3621
Size: 1994 2015 378 5934
Fragments arranged by size:
5934 2015 1994 378
EcoRV GAT'ATC
Cuts at: 3244 3244
Size: 10321
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2215 2215
Size: 10321
FspI TGC'GCA
Cuts at: 4001 5093 5591 8827 4001
Size: 1092 498 3236 5495
Fragments arranged by size:
5495 3236 1092 498
FspAI rTGC'GCAy
Cuts at: 4001 5093 5591 4001
Size: 1092 498 8731
Fragments arranged by size:
8731 1092 498
HaeII r_GCGC'y
Cuts at: 1678 6136 7519 9468 1678
Size: 4458 1383 1949 2531
Fragments arranged by size:
4458 2531 1949 1383
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2461 2493 2555 2569 2587
Size: 32 135 32 889 32 62 14 18
Cuts at: 2587 2601 2932 2964 3271 3303 3496 3528 5258
Size: 14 331 32 307 32 193 32 1730
Cuts at: 5258 5290 5481 5513 6625 6657 7247 7279 7415
Size: 32 191 32 1112 32 590 32 136
Cuts at: 7415 7447 7568 7595 7600 7627 8962 8994 9036
Size: 32 121 27 5 27 1335 32 42
Cuts at: 9036 9068 1373
Size: 32 2626
Fragments arranged by size:
2626 1730 1335 1112 889 590 331 307
193 191 136 135 121 62 42 32
32 32 32 32 32 32 32 32
32 32 32 32 27 27 18 14
14 5
HincII GTy'rAC
Cuts at: 669 1657 1938 5103 7665 7911 669
Size: 988 281 3165 2562 246 3079
Fragments arranged by size:
3165 3079 2562 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3969 5729 7450 748
Size: 3221 1760 1721 3619
Fragments arranged by size:
3619 3221 1760 1721
HpaI GTT'AAC
Cuts at: 7911 7911
Size: 10321
KasI G'GCGC_C
Cuts at: 6132 6132
Size: 10321
KpnI G_GTAC'C
Cuts at: 7646 7646
Size: 10321
MfeI C'AATT_G
Cuts at: 4217 7920 4217
Size: 3703 6618
MluI A'CGCG_T
Cuts at: 10197 10197
Size: 10321
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3468 3937 5892 5898 6058 9435 9619 1305
Size: 2163 469 1955 6 160 3377 184 2007
Fragments arranged by size:
3377 2163 2007 1955 469 184 160 6
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4215 4353 6583 6734 10
Size: 54 1425 343 2383 138 2230 151 3597
Fragments arranged by size:
3597 2383 2230 1425 343 151 138 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2942 3821 4571 5442 7313 8279 8638
Size: 648 1776 879 750 871 1871 966 359
Cuts at: 8638 8797 518
Size: 159 2042
Fragments arranged by size:
2042 1871 1776 966 879 871 750 648
359 159
MspA1I CmG'CkG
Cuts at: 1606 2096 2158 3226 5070 5279 5570 7231 7737
Size: 490 62 1068 1844 209 291 1661 506
Cuts at: 7737 8414 9562 9807 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1844 1661 1148 1068 677 506 490
291 245 209 62
NarI GG'CG_CC
Cuts at: 6133 6133
Size: 10321
NcoI C'CATG_G
Cuts at: 513 1065 2004 3695 7375 513
Size: 552 939 1691 3680 3459
Fragments arranged by size:
3680 3459 1691 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 10321
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10321
NotI GC'GGCC_GC
Cuts at: 7768 7768
Size: 10321
NspI r_CATG'y
Cuts at: 1132 1229 3673 5326 5805 7297 7679 9224 1132
Size: 97 2444 1653 479 1492 382 1545 2229
Fragments arranged by size:
2444 2229 1653 1545 1492 479 382 97
PasI CC'CwG_GG
Cuts at: 1348 6920 1348
Size: 5572 4749
PciI A'CATG_T
Cuts at: 3669 5322 9220 3669
Size: 1653 3898 4770
Fragments arranged by size:
4770 3898 1653
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2639 2836 7381 1108
Size: 1531 197 4545 4048
Fragments arranged by size:
4545 4048 1531 197
PfoI T'CCnGG_A
Cuts at: 2829 4138 2829
Size: 1309 9012
PmeI GTTT'AAAC
Cuts at: 7625 7625
Size: 10321
PmlI CAC'GTG
Cuts at: 5360 5360
Size: 10321
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8390 8422 9903 9935 8390
Size: 32 1481 32 8776
Fragments arranged by size:
8776 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2009 2582 3899 4012 5738 2009
Size: 573 1317 113 1726 6592
Fragments arranged by size:
6592 1726 1317 573 113
PshAI GACnn'nnGTC
Cuts at: 1613 4989 1613
Size: 3376 6945
PsiI TTA'TAA
Cuts at: 4116 7891 4116
Size: 3775 6546
PspOMI G'GGCC_C
Cuts at: 3827 5129 6923 3827
Size: 1302 1794 7225
Fragments arranged by size:
7225 1794 1302
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2873 2905 6788 6820 2873
Size: 32 3883 32 6374
Fragments arranged by size:
6374 3883 32 32
PstI C_TGCA'G
Cuts at: 830 4687 7673 830
Size: 3857 2986 3478
Fragments arranged by size:
3857 3478 2986
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10321
PvuII CAG'CTG
Cuts at: 2158 3226 5279 5570 7231 2158
Size: 1068 2053 291 1661 5248
Fragments arranged by size:
5248 2053 1661 1068 291
SalI G'TCGA_C
Cuts at: 1936 7663 1936
Size: 5727 4594
SanDI GG'GwC_CC
Cuts at: 2009 2009
Size: 10321
SapI GCTCTTCn'nnn_
Cuts at: 2814 7503 2814
Size: 4689 5632
SbfI CC_TGCA'GG
Cuts at: 7673 7673
Size: 10321
ScaI AGT'ACT
Cuts at: 3872 8569 10281 3872
Size: 4697 1712 3912
Fragments arranged by size:
4697 3912 1712
SfcI C'TryA_G
Cuts at: 826 1045 4424 4683 5219 5390 7669 8804 9485
Size: 219 3379 259 536 171 2279 1135 681
Cuts at: 9485 9676 826
Size: 191 1471
Fragments arranged by size:
3379 2279 1471 1135 681 536 259 219
191 171
SfoI GGC'GCC
Cuts at: 6134 6134
Size: 10321
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10321
SgrAI Cr'CCGG_yG
Cuts at: 1700 6129 1700
Size: 4429 5892
SmaI CCC'GGG
Cuts at: 1872 2493 7648 1872
Size: 621 5155 4545
Fragments arranged by size:
5155 4545 621
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3167 4595 5498 5705 5732 6032
Size: 197 926 1224 1428 903 207 27 300
Cuts at: 6032 6845 6981 7125 8425 9326 9588 820
Size: 813 136 144 1300 901 262 1553
Fragments arranged by size:
1553 1428 1300 1224 926 903 901 813
300 262 207 197 144 136 27
SnaBI TAC'GTA
Cuts at: 493 5027 6665 8112 493
Size: 4534 1638 1447 2702
Fragments arranged by size:
4534 2702 1638 1447
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10321
SphI G_CATG'C
Cuts at: 1132 7297 7679 1132
Size: 6165 382 3774
Fragments arranged by size:
6165 3774 382
SspI AAT'ATT
Cuts at: 5 52 3766 8245 5
Size: 47 3714 4479 2081
Fragments arranged by size:
4479 3714 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 10321
StyI C'CwwG_G
Cuts at: 513 1065 2004 2283 3392 3582 3695 4796 7375
Size: 552 939 279 1109 190 113 1101 2579
Cuts at: 7375 7760 513
Size: 385 3074
Fragments arranged by size:
3074 2579 1109 1101 939 552 385 279
190 113
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3053 3833 4579 6566 6609 7454 8348
Size: 1255 1473 780 746 1987 43 845 894
Cuts at: 8348 8365 8518 8703 9148 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1987 1498 1473 1255 894 845 780 746
445 185 153 43 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2312 3870 6860
Size: 274 80 33 51 1443 335 1558 2990
Cuts at: 6860 7114 8567 10279 96
Size: 254 1453 1712 138
Fragments arranged by size:
2990 1712 1558 1453 1443 335 274 254
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2263 2295 2825 2857 10281 10313 2263
Size: 32 530 32 7424 32 2271
Fragments arranged by size:
7424 2271 530 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5493 6157 1316
Size: 4177 664 5480
Fragments arranged by size:
5480 4177 664
XbaI T'CTAG_A
Cuts at: 3140 7657 3140
Size: 4517 5804
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3013 5974 7589 3013
Size: 2961 1615 5745
Fragments arranged by size:
5745 2961 1615
XhoI C'TCGA_G
Cuts at: 1943 6981 1943
Size: 5038 5283
XmaI C'CCGG_G
Cuts at: 1870 2491 7646 1870
Size: 621 5155 4545
Fragments arranged by size:
5155 4545 621
XmnI GAAnn'nnTTC
Cuts at: 2691 5619 8450 2691
Size: 2928 2831 4562
Fragments arranged by size:
4562 2928 2831
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 8129 276
Size: 53 83 186 936 6595 2468
Fragments arranged by size:
6595 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PsrI PstI PvuI PvuII SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BfrBI BsiWI EcoICRI FseI
NaeI NgoMIV NruI NsiI PacI PspXI RsrII SacI
SacII SexAI SfiI SrfI SwaI