Restriction map for
pFN21AE5838
(Circular) (Six-base) MAPSORT of: pFN21AE5838.seq Check: 9887 from: 1 to: 9826
pFN21AE5838 9826 bp
With 182 enzymes: SgfI *
August 16, 2021 15:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5948 5948
Size: 9826
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7636 278
Size: 53 83 186 936 6100 2468
Fragments arranged by size:
6100 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3405 7169 1937
Size: 1468 3764 4594
Fragments arranged by size:
4594 3764 1468
Acc65I G'GTAC_C
Cuts at: 7147 7147
Size: 9826
AclI AA'CG_TT
Cuts at: 1563 7125 7953 8326 1563
Size: 5562 828 373 3063
Fragments arranged by size:
5562 3063 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2198 2306 4205 4882 5440 5596 5652 6408
Size: 566 108 1899 677 558 156 56 756
Cuts at: 6408 7889 9273 9541 1632
Size: 1481 1384 268 1917
Fragments arranged by size:
1917 1899 1481 1384 756 677 566 558
268 156 108 56
AfeI AGC'GCT
Cuts at: 3737 3737
Size: 9826
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9629
AflIII A'CryG_T
Cuts at: 1203 4254 4281 5685 8725 9702 1203
Size: 3051 27 1404 3040 977 1327
Fragments arranged by size:
3051 3040 1404 1327 977 27
AgeI A'CCGG_T
Cuts at: 4563 8657 4563
Size: 4094 5732
AhdI GACnn_n'nnGTC
Cuts at: 2365 7066 8555 2365
Size: 4701 1489 3636
Fragments arranged by size:
4701 3636 1489
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9826
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4283 1184
Size: 3099 6727
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5615 5647 5615
Size: 32 9794
AlwNI CAG_nnn'CTG
Cuts at: 3235 4081 4270 5485 6152 7569 9141 3235
Size: 846 189 1215 667 1417 1572 3920
Fragments arranged by size:
3920 1572 1417 1215 846 667 189
ApaI G_GGCC'C
Cuts at: 3597 4991 3597
Size: 1394 8432
ApaLI G'TGCA_C
Cuts at: 7883 9039 7883
Size: 1156 8670
ApoI r'AATT_y
Cuts at: 1493 2077 4338 4595 7135 7361 7513 9622 1493
Size: 584 2261 257 2540 226 152 2109 1697
Fragments arranged by size:
2540 2261 2109 1697 584 257 226 152
AseI AT'TA_AT
Cuts at: 160 1032 8380 160
Size: 872 7348 1606
Fragments arranged by size:
7348 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9826
AvaI C'yCGr_G
Cuts at: 1870 1943 2730 5214 5851 7151 1870
Size: 73 787 2484 637 1300 4545
Fragments arranged by size:
4545 2484 1300 787 637 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 6291 6324 6291
Size: 33 9793
BamHI G'GATC_C
Cuts at: 7156 7523 7156
Size: 367 9459
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 6613 7147 8602 618
Size: 325 217 627 4826 534 1455 1842
Fragments arranged by size:
4826 1842 1455 627 534 325 217
BanII G_rGCy'C
Cuts at: 1391 2625 3597 3921 4125 4491 4991 5568 6234
Size: 1234 972 324 204 366 500 577 666
Cuts at: 6234 7145 1391
Size: 911 4072
Fragments arranged by size:
4072 1234 972 911 666 577 500 366
324 204
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2941 3206 5781 6193 928
Size: 971 1042 265 2575 412 4561
Fragments arranged by size:
4561 2575 1042 971 412 265
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 8017 8051 1903
Size: 34 50 34 5996 34 3678
Fragments arranged by size:
5996 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4643 5649 7718 8928 4643
Size: 1006 2069 1210 5541
Fragments arranged by size:
5541 2069 1210 1006
BclI T'GATC_A
Cuts at: 1548 5448 1548
Size: 3900 5926
BfrBI ATG'CAT
Cuts at: 2869 5598 2869
Size: 2729 7097
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4007 5282 8437
Size: 107 122 71 631 605 2335 1275 3155
Cuts at: 8437 136
Size: 1525
Fragments arranged by size:
3155 2335 1525 1275 631 605 122 107
71
BglII A'GATC_T
Cuts at: 3423 6413 9821 3423
Size: 2990 3408 3428
Fragments arranged by size:
3428 3408 2990
BlpI GC'TnA_GC
Cuts at: 3228 3382 7243 3228
Size: 154 3861 5811
Fragments arranged by size:
5811 3861 154
Bme1580I G_kGCm'C
Cuts at: 1790 3597 4991 7887 9043 1790
Size: 1807 1394 2896 1156 2573
Fragments arranged by size:
2896 2573 1807 1394 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9826
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2301 7557 8515
Size: 416 53 83 399 24 884 5256 958
Cuts at: 8515 442
Size: 1753
Fragments arranged by size:
5256 1753 958 884 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9826
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2573 2667 2685 2945 3482 3544 4213 4510
Size: 1334 94 18 260 537 62 669 297
Cuts at: 4510 5720 5726 5825 5843 6288 6732 8486 1239
Size: 1210 6 99 18 445 444 1754 2579
Fragments arranged by size:
2579 1754 1334 1210 669 537 445 444
297 260 99 94 62 18 18 6
Bpu10I CC'TnA_GC
Cuts at: 2046 6089 2046
Size: 4043 5783
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2585 3841 4307 5552 6571 6666 7951 8816 9114
Size: 1256 466 1245 1019 95 1285 865 298
Cuts at: 9114 2585
Size: 3297
Fragments arranged by size:
3297 1285 1256 1245 1019 865 466 298
95
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5204 5960 8489 882
Size: 617 3705 756 2529 2219
Fragments arranged by size:
3705 2529 2219 756 617
BsaAI yAC'GTr
Cuts at: 493 1204 4206 4257 7617 493
Size: 711 3002 51 3360 2702
Fragments arranged by size:
3360 3002 2702 711 51
BsaBI GATnn'nnATC
Cuts at: 3674 4721 9820 3674
Size: 1047 5099 3680
Fragments arranged by size:
5099 3680 1047
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2275 5877 7633 8015
Size: 53 83 186 936 742 3602 1756 382
Cuts at: 8015 8650 275
Size: 635 1451
Fragments arranged by size:
3602 1756 1451 936 742 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2133 3741 4563 8259 8657 8931 9078 9489 2133
Size: 1608 822 3696 398 274 147 411 2470
Fragments arranged by size:
3696 2470 1608 822 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2484 2514 2907 2937 5672
Size: 30 165 30 1081 30 393 30 2735
Cuts at: 5672 5702 5957 5987 6383 6413 6714 6744 1178
Size: 30 255 30 396 30 301 30 4260
Fragments arranged by size:
4260 2735 1081 396 393 301 255 165
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2100 2492 3998 5120 5315 5978 6812 6941
Size: 914 392 1506 1122 195 663 834 129
Cuts at: 6941 7090 1186
Size: 149 3922
Fragments arranged by size:
3922 1506 1122 914 834 663 392 195
149 129
BseYI C'CCAG_C
Cuts at: 2240 3796 6280 6782 9029 9525 2240
Size: 1556 2484 502 2247 496 2541
Fragments arranged by size:
2541 2484 2247 1556 502 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4279 5854 1163
Size: 573 2543 1575 5135
Fragments arranged by size:
5135 2543 1575 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7276 8037 9065 1540
Size: 275 186 5275 761 1028 2301
Fragments arranged by size:
5275 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3921 4125 4491 5568 5724 6234 7887 7972 9043
Size: 204 366 1077 156 510 1653 85 1071
Cuts at: 9043 3921
Size: 4704
Fragments arranged by size:
4704 1653 1077 1071 510 366 204 156
85
BsmI GAATG_Cn'
Cuts at: 2869 6452 7337 7430 2869
Size: 3583 885 93 5265
Fragments arranged by size:
5265 3583 885 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4886 4958 4886
Size: 72 9754
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2625 3597 3921 4125 4491 4991 5568
Size: 399 835 972 324 204 366 500 577
Cuts at: 5568 5724 6234 7145 7887 7972 9043 1391
Size: 156 510 911 742 85 1071 2174
Fragments arranged by size:
2174 1071 972 911 835 742 577 510
500 399 366 324 204 156 85
BspEI T'CCGG_A
Cuts at: 2133 3741 9489 2133
Size: 1608 5748 2470
Fragments arranged by size:
5748 2470 1608
BspHI T'CATG_A
Cuts at: 2091 3939 4036 5409 7715 9445 2091
Size: 1848 97 1373 2306 1730 2472
Fragments arranged by size:
2472 2306 1848 1730 1373 97
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3651 5948 7181 844
Size: 2807 2297 1233 3489
Fragments arranged by size:
3489 2807 2297 1233
BsrBI CCG'CTC
Cuts at: 1394 1823 5827 5850 7713 1394
Size: 429 4004 23 1863 3507
Fragments arranged by size:
4004 3507 1863 429 23
BsrDI GCAATG_nn'
Cuts at: 65 1248 2040 2643 4503 5328 5600 6828 8321
Size: 1183 792 603 1860 825 272 1228 1493
Cuts at: 8321 8495 65
Size: 174 1396
Fragments arranged by size:
1860 1493 1396 1228 1183 825 792 603
272 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3448 4293 4563 5272 8470 8657 1700
Size: 110 1638 845 270 709 3198 187 2869
Fragments arranged by size:
3198 2869 1638 845 709 270 187 110
BsrGI T'GTAC_A
Cuts at: 96 3576 96
Size: 3480 6346
BssHII G'CGCG_C
Cuts at: 1925 6904 1925
Size: 4979 4847
BssSI C'ACGA_G
Cuts at: 5507 7886 8898 5507
Size: 2379 1012 6435
Fragments arranged by size:
6435 2379 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4020 6152 6347 794
Size: 3226 2132 195 4273
Fragments arranged by size:
4273 3226 2132 195
BstBI TT'CG_AA
Cuts at: 2932 8630 2932
Size: 5698 4128
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9826
BstXI CCAn_nnnn'nTGG
Cuts at: 2016 2294 2444 4003 5803 6163 2016
Size: 278 150 1559 1800 360 5679
Fragments arranged by size:
5679 1800 1559 360 278 150
BstYI r'GATC_y
Cuts at: 1972 3423 3455 6413 7156 7523 7908 7925 9377
Size: 1451 32 2958 743 367 385 17 1452
Cuts at: 9377 9463 9475 9821 1972
Size: 86 12 346 1977
Fragments arranged by size:
2958 1977 1452 1451 743 385 367 346
86 32 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2015 2563 3990 4029 4221 5748 5796
Size: 552 950 548 1427 39 192 1527 48
Cuts at: 5796 6402 513
Size: 606 3937
Fragments arranged by size:
3937 1527 1427 950 606 552 548 192
48 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2715 3394 4231 4828 487
Size: 683 56 1489 679 837 597 5485
Fragments arranged by size:
5485 1489 837 683 679 597 56
BtsI GCAGTG_nn'
Cuts at: 7342 8136 8156 7342
Size: 794 20 9012
Fragments arranged by size:
9012 794 20
ClaI AT'CG_AT
Cuts at: 1554 5366 1554
Size: 3812 6014
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9791
DraI TTT'AAA
Cuts at: 6076 7130 6076
Size: 1054 8772
DraIII CAC_nnn'GTG
Cuts at: 6666 6666
Size: 9826
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4913 6212 8833 809
Size: 507 3597 1299 2621 1802
Fragments arranged by size:
3597 2621 1802 1299 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2446 2813 3446
Size: 54 1425 50 275 18 616 367 633
Cuts at: 3446 3993 4105 4158 4697 5124 5274 5793 6165
Size: 547 112 53 539 427 150 519 372
Cuts at: 6165 7273 8162 9791 8
Size: 1108 889 1629 43
Fragments arranged by size:
1629 1425 1108 889 633 616 547 539
519 427 372 367 275 150 112 54
53 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 7273 1537
Size: 275 5461 4090
Fragments arranged by size:
5461 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2794 3767 3852 4470 4536 4667 5552 5583
Size: 1441 973 85 618 66 131 885 31
Cuts at: 5583 6537 6953 7756 1353
Size: 954 416 803 3423
Fragments arranged by size:
3423 1441 973 954 885 803 618 416
131 85 66 31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3165 8413 8787 8933 387
Size: 1064 138 1576 5248 374 146 1280
Fragments arranged by size:
5248 1576 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3919 4123 4489 5566 6232 3919
Size: 204 366 1077 666 7513
Fragments arranged by size:
7513 1077 666 366 204
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2198 2306 2573 2667 2685 2945 3482
Size: 393 566 108 267 94 18 260 537
Cuts at: 3482 3544 4205 4213 4510 4882 5440 5596 5652
Size: 62 661 8 297 372 558 156 56
Cuts at: 5652 5720 5726 5825 5843 6288 6408 6732 7889
Size: 68 6 99 18 445 120 324 1157
Cuts at: 7889 8486 9273 9541 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1157 787 661 597 566 558 537
445 393 372 324 297 268 267 260
156 120 108 99 94 68 62 56
18 18 8 6
EcoO109I rG'GnC_Cy
Cuts at: 2569 2788 3023 3594 4000 5856 6084 2569
Size: 219 235 571 406 1856 228 6311
Fragments arranged by size:
6311 1856 571 406 235 228 219
EcoRI G'AATT_C
Cuts at: 4338 4595 7135 7513 4338
Size: 257 2540 378 6651
Fragments arranged by size:
6651 2540 378 257
EcoRV GAT'ATC
Cuts at: 5370 5370
Size: 9826
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2626 5108 5152 6033 2626
Size: 2482 44 881 6419
Fragments arranged by size:
6419 2482 881 44
FspI TGC'GCA
Cuts at: 4307 8332 4307
Size: 4025 5801
HaeII r_GCGC'y
Cuts at: 1678 3739 5812 8973 1678
Size: 2061 2073 3161 2531
Fragments arranged by size:
3161 2531 2073 2061
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2211 2243 2351 2383 2705
Size: 32 135 32 639 32 108 32 322
Cuts at: 2705 2737 2793 2825 3393 3425 4864 4896 5096
Size: 32 56 32 568 32 1439 32 200
Cuts at: 5096 5128 5176 5208 5250 5282 5672 5704 5957
Size: 32 48 32 42 32 390 32 253
Cuts at: 5957 5989 6383 6398 6415 6430 7052 7084 8467
Size: 32 394 15 17 15 622 32 1383
Cuts at: 8467 8499 8541 8573 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1439 1383 639 622 568 394 390
322 253 200 135 108 56 48 42
42 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 17 15 15
HincII GTy'rAC
Cuts at: 669 1657 1938 2674 3406 4063 5320 5710 7170
Size: 988 281 736 732 657 1257 390 1460
Cuts at: 7170 7416 669
Size: 246 3079
Fragments arranged by size:
3079 1460 1257 988 736 732 657 390
281 246
HindIII A'AGCT_T
Cuts at: 748 2546 6292 7105 748
Size: 1798 3746 813 3469
Fragments arranged by size:
3746 3469 1798 813
HpaI GTT'AAC
Cuts at: 2674 7416 2674
Size: 4742 5084
KpnI G_GTAC'C
Cuts at: 7151 7151
Size: 9826
MfeI C'AATT_G
Cuts at: 6687 7425 6687
Size: 738 9088
MluI A'CGCG_T
Cuts at: 9702 9702
Size: 9826
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2478 2514 3456 4846 5397 5862 6011 6042
Size: 1173 36 942 1390 551 465 149 31
Cuts at: 6042 6900 8940 9124 1305
Size: 858 2040 184 2007
Fragments arranged by size:
2040 2007 1390 1173 942 858 551 465
184 149 36 31
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2448 3995 4107 4160 5126
Size: 54 1425 343 616 1547 112 53 966
Cuts at: 5126 5795 6167 10
Size: 669 372 3669
Fragments arranged by size:
3669 1547 1425 966 669 616 372 343
112 54 53
MslI CAynn'nnrTG
Cuts at: 518 1166 2468 3248 4280 4286 4496 5045 5468
Size: 648 1302 780 1032 6 210 549 423
Cuts at: 5468 5690 6196 6401 7784 8143 8302 518
Size: 222 506 205 1383 359 159 2042
Fragments arranged by size:
2042 1383 1302 1032 780 648 549 506
423 359 222 210 205 159 6
MspA1I CmG'CkG
Cuts at: 1606 3181 4111 4156 5239 5770 6994 7242 7919
Size: 1575 930 45 1083 531 1224 248 677
Cuts at: 7919 9067 9312 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1575 1224 1148 1083 930 677 531
248 245 45
NaeI GCC'GGC
Cuts at: 4295 5274 4295
Size: 979 8847
NcoI C'CATG_G
Cuts at: 513 1065 2563 3990 4029 6402 513
Size: 552 1498 1427 39 2373 3937
Fragments arranged by size:
3937 2373 1498 1427 552 39
NdeI CA'TA_TG
Cuts at: 387 6943 387
Size: 6556 3270
NgoMIV G'CCGG_C
Cuts at: 4293 5272 4293
Size: 979 8847
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9826
NotI GC'GGCC_GC
Cuts at: 7273 7273
Size: 9826
NsiI A_TGCA'T
Cuts at: 2871 5600 2871
Size: 2729 7097
NspI r_CATG'y
Cuts at: 1132 1229 2294 2869 3541 4285 4306 4393 5146
Size: 97 1065 575 672 744 21 87 753
Cuts at: 5146 5689 7184 8729 1132
Size: 543 1495 1545 2229
Fragments arranged by size:
2229 1545 1495 1065 753 744 672 575
543 97 87 21
PasI CC'CwG_GG
Cuts at: 1348 2763 4741 6459 1348
Size: 1415 1978 1718 4715
Fragments arranged by size:
4715 1978 1718 1415
PciI A'CATG_T
Cuts at: 4281 5685 8725 4281
Size: 1404 3040 5382
Fragments arranged by size:
5382 3040 1404
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2526 3184 5749 1108
Size: 1418 658 2565 5185
Fragments arranged by size:
5185 2565 1418 658
PfoI T'CCnGG_A
Cuts at: 2192 4125 4526 2192
Size: 1933 401 7492
Fragments arranged by size:
7492 1933 401
PmeI GTTT'AAAC
Cuts at: 7130 7130
Size: 9826
PmlI CAC'GTG
Cuts at: 4206 4257 4206
Size: 51 9775
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3211 3243 7895 7927 9408 9440 3211
Size: 32 4652 32 1481 32 3597
Fragments arranged by size:
4652 3597 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2788 3023 5856 6084 2788
Size: 235 2833 228 6530
Fragments arranged by size:
6530 2833 235 228
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9826
PsiI TTA'TAA
Cuts at: 2214 7396 2214
Size: 5182 4644
PspOMI G'GGCC_C
Cuts at: 3593 4987 3593
Size: 1394 8432
PspXI vC'TCGA_Gb
Cuts at: 5851 5851
Size: 9826
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4922 4954 5123 5155 4922
Size: 32 169 32 9593
Fragments arranged by size:
9593 169 32 32
PstI C_TGCA'G
Cuts at: 830 2577 4215 4818 5959 6148 7178 830
Size: 1747 1638 603 1141 189 1030 3478
Fragments arranged by size:
3478 1747 1638 1141 1030 603 189
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9826
PvuII CAG'CTG
Cuts at: 3181 4111 4156 5239 5770 3181
Size: 930 45 1083 531 7237
Fragments arranged by size:
7237 1083 930 531 45
SacI G_AGCT'C
Cuts at: 3921 4125 4491 5568 6234 3921
Size: 204 366 1077 666 7513
Fragments arranged by size:
7513 1077 666 366 204
SalI G'TCGA_C
Cuts at: 1936 3404 7168 1936
Size: 1468 3764 4594
Fragments arranged by size:
4594 3764 1468
SapI GCTCTTCn'nnn_
Cuts at: 4667 5583 6953 4667
Size: 916 1370 7540
Fragments arranged by size:
7540 1370 916
SbfI CC_TGCA'GG
Cuts at: 5959 7178 5959
Size: 1219 8607
ScaI AGT'ACT
Cuts at: 4823 8074 9786 4823
Size: 3251 1712 4863
Fragments arranged by size:
4863 3251 1712
SfcI C'TryA_G
Cuts at: 826 1045 2573 3655 4211 4793 4814 5955 6120
Size: 219 1528 1082 556 582 21 1141 165
Cuts at: 6120 6144 7174 8309 8990 9181 826
Size: 24 1030 1135 681 191 1471
Fragments arranged by size:
1528 1471 1141 1135 1082 1030 681 582
556 219 191 165 24 21
SfiI GGCCn_nnn'nGGCC
Cuts at: 4007 5282 4007
Size: 1275 8551
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9826
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9826
SmaI CCC'GGG
Cuts at: 1872 7153 1872
Size: 5281 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2600 2730 3820 4322 5567 5851
Size: 197 926 657 130 1090 502 1245 284
Cuts at: 5851 6586 6681 7930 8831 9093 820
Size: 735 95 1249 901 262 1553
Fragments arranged by size:
1553 1249 1245 1090 926 901 735 657
502 284 262 197 130 95
SnaBI TAC'GTA
Cuts at: 493 7617 493
Size: 7124 2702
SpeI A'CTAG_T
Cuts at: 152 6107 152
Size: 5955 3871
SphI G_CATG'C
Cuts at: 1132 2869 3541 4306 7184 1132
Size: 1737 672 765 2878 3774
Fragments arranged by size:
3774 2878 1737 765 672
SspI AAT'ATT
Cuts at: 5 52 7750 5
Size: 47 7698 2081
Fragments arranged by size:
7698 2081 47
StuI AGG'CCT
Cuts at: 1517 4043 4320 1517
Size: 2526 277 7023
Fragments arranged by size:
7023 2526 277
StyI C'CwwG_G
Cuts at: 513 1065 2437 2520 2563 3990 3996 4029 6162
Size: 552 1372 83 43 1427 6 33 2133
Cuts at: 6162 6274 6402 7265 513
Size: 112 128 863 3074
Fragments arranged by size:
3074 2133 1427 1372 863 552 128 112
83 43 33 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2972 4624 4721 6016 6367 6449 7853
Size: 1255 1392 1652 97 1295 351 82 1404
Cuts at: 7853 7870 8023 8208 8653 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1652 1498 1404 1392 1295 1255 445 351
185 153 97 82 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3576 4821 5031
Size: 274 80 33 51 1443 1599 1245 210
Cuts at: 5031 5575 8072 9784 96
Size: 544 2497 1712 138
Fragments arranged by size:
2497 1712 1599 1443 1245 544 274 210
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2907 2939 3015 3047 6714 6746 9786 9818 2907
Size: 32 76 32 3667 32 3040 32 2915
Fragments arranged by size:
3667 3040 2915 76 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2827 5860 5971 1316
Size: 1511 3033 111 5171
Fragments arranged by size:
5171 3033 1511 111
XbaI T'CTAG_A
Cuts at: 6538 7162 6538
Size: 624 9202
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4003 4003
Size: 9826
XhoI C'TCGA_G
Cuts at: 1943 2730 5851 1943
Size: 787 3121 5918
Fragments arranged by size:
5918 3121 787
XmaI C'CCGG_G
Cuts at: 1870 7151 1870
Size: 5281 4545
XmnI GAAnn'nnTTC
Cuts at: 3203 5135 5546 5927 6013 6962 7955 3203
Size: 1932 411 381 86 949 993 5074
Fragments arranged by size:
5074 1932 993 949 411 381 86
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7634 276
Size: 53 83 186 936 6100 2468
Fragments arranged by size:
6100 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbsI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BbeI BbvCI BplI BsiWI BstZ17I Bsu36I
EcoNI FseI FspAI KasI NarI NruI PacI RsrII
SacII SanDI SexAI SfoI SrfI SwaI