Restriction map for
pFN21AE5837
(Circular) (Six-base) MAPSORT of: pFN21AE5837.seq Check: 6167 from: 1 to: 9751
pFN21AE5837 9751 bp
With 182 enzymes: SgfI *
December 8, 2014 13:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3028 3946 4667 4858 6517 3028
Size: 918 721 191 1659 6262
Fragments arranged by size:
6262 1659 918 721 191
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7561 278
Size: 53 83 186 936 6025 2468
Fragments arranged by size:
6025 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2223 6030 7094 1937
Size: 286 3807 1064 4594
Fragments arranged by size:
4594 3807 1064 286
Acc65I G'GTAC_C
Cuts at: 2056 7072 2056
Size: 5016 4735
AclI AA'CG_TT
Cuts at: 1563 3074 3321 7878 8251 1563
Size: 1511 247 4557 373 3063
Fragments arranged by size:
4557 3063 1511 373 247
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2594 2637 2967 4605 5496 5823 6290 6415
Size: 962 43 330 1638 891 327 467 125
Cuts at: 6415 6640 6947 7021 7049 7814 9198 9466 1632
Size: 225 307 74 28 765 1384 268 1917
Fragments arranged by size:
1917 1638 1384 962 891 765 467 330
327 307 268 225 125 74 43 28
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9554
AflIII A'CryG_T
Cuts at: 1203 2065 2226 3612 4625 8650 9627 1203
Size: 862 161 1386 1013 4025 977 1327
Fragments arranged by size:
4025 1386 1327 1013 977 862 161
AgeI A'CCGG_T
Cuts at: 6909 8582 6909
Size: 1673 8078
AhdI GACnn_n'nnGTC
Cuts at: 8480 8480
Size: 9751
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9751
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4263 1184
Size: 3079 6672
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4423 4455 4423
Size: 32 9719
AlwNI CAG_nnn'CTG
Cuts at: 3971 4130 4474 4778 6977 7027 7494 9066 3971
Size: 159 344 304 2199 50 467 1572 4656
Fragments arranged by size:
4656 2199 1572 467 344 304 159 50
ApaLI G'TGCA_C
Cuts at: 5659 7808 8964 5659
Size: 2149 1156 6446
Fragments arranged by size:
6446 2149 1156
ApoI r'AATT_y
Cuts at: 1493 2168 2248 2338 2911 3379 3409 3723 4595
Size: 675 80 90 573 468 30 314 872
Cuts at: 4595 5498 5600 5959 7060 7286 7438 9547 1493
Size: 903 102 359 1101 226 152 2109 1697
Fragments arranged by size:
2109 1697 1101 903 872 675 573 468
359 314 226 152 102 90 80 30
AseI AT'TA_AT
Cuts at: 160 1032 8305 160
Size: 872 7273 1606
Fragments arranged by size:
7273 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9751
AvaI C'yCGr_G
Cuts at: 1870 1943 5141 7076 1870
Size: 73 3198 1935 4545
Fragments arranged by size:
4545 3198 1935 73
BamHI G'GATC_C
Cuts at: 7081 7448 7081
Size: 367 9384
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2056 2707 3234 7072 8527
Size: 325 217 627 269 651 527 3838 1455
Cuts at: 8527 618
Size: 1842
Fragments arranged by size:
3838 1842 1455 651 627 527 325 269
217
BanII G_rGCy'C
Cuts at: 1391 2412 2498 3576 3935 4394 4742 4796 5121
Size: 1021 86 1078 359 459 348 54 325
Cuts at: 5121 5352 6231 6613 7070 1391
Size: 231 879 382 457 4072
Fragments arranged by size:
4072 1078 1021 879 459 457 382 359
348 325 231 86 54
BbeI G_GCGC'C
Cuts at: 3238 3238
Size: 9751
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2439 2582 2643 2880 3006 3995 928
Size: 971 540 143 61 237 126 989 6684
Fragments arranged by size:
6684 989 971 540 237 143 126 61
BbvCI CC'TCA_GC
Cuts at: 2052 2659 4506 2052
Size: 607 1847 7297
Fragments arranged by size:
7297 1847 607
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7942 7976 1903
Size: 34 6005 34 3678
Fragments arranged by size:
6005 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7643 8853 7643
Size: 1210 8541
BclI T'GATC_A
Cuts at: 1548 3372 1548
Size: 1824 7927
BfrBI ATG'CAT
Cuts at: 3142 6266 3142
Size: 3124 6627
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6208 8362 136
Size: 107 122 71 631 605 4536 2154 1525
Fragments arranged by size:
4536 2154 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2201 3871 4549 5446 5638 9746 2201
Size: 1670 678 897 192 4108 2206
Fragments arranged by size:
4108 2206 1670 897 678 192
BlpI GC'TnA_GC
Cuts at: 4767 6230 6807 7168 4767
Size: 1463 577 361 7350
Fragments arranged by size:
7350 1463 577 361
Bme1580I G_kGCm'C
Cuts at: 1790 5663 7812 8968 1790
Size: 3873 2149 1156 2573
Fragments arranged by size:
3873 2573 2149 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9751
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3345 4221 5347
Size: 416 53 83 399 24 1928 876 1126
Cuts at: 5347 6107 7482 8440 442
Size: 760 1375 958 1753
Fragments arranged by size:
1928 1753 1375 1126 958 876 760 416
399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9751
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6379 6379
Size: 9751
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2669 2697 3333 3567 3798 4509 5297 6715
Size: 1430 28 636 234 231 711 788 1418
Cuts at: 6715 8411 1239
Size: 1696 2579
Fragments arranged by size:
2579 1696 1430 1418 788 711 636 234
231 28
Bpu10I CC'TnA_GC
Cuts at: 2052 2323 2659 4506 6619 2052
Size: 271 336 1847 2113 5184
Fragments arranged by size:
5184 2113 1847 336 271
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2687 3149 4177 7006 7876 8741 9039 2687
Size: 462 1028 2829 870 865 298 3399
Fragments arranged by size:
3399 2829 1028 870 865 462 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2036 8414 882
Size: 617 537 6378 2219
Fragments arranged by size:
6378 2219 617 537
BsaAI yAC'GTr
Cuts at: 493 1204 4626 6841 7542 493
Size: 711 3422 2215 701 2702
Fragments arranged by size:
3422 2702 2215 711 701
BsaBI GATnn'nnATC
Cuts at: 9745 9745
Size: 9751
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3235 7558 7940 8575
Size: 53 83 186 936 1702 4323 382 635
Cuts at: 8575 275
Size: 1451
Fragments arranged by size:
4323 1702 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2083 4877 6909 8184 8582 8856 9003 9414 2083
Size: 2794 2032 1275 398 274 147 411 2420
Fragments arranged by size:
2794 2420 2032 1275 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3306 3336 4204 4234 4729
Size: 30 165 30 1903 30 868 30 495
Cuts at: 4729 4759 1178
Size: 30 6170
Fragments arranged by size:
6170 1903 868 495 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2420 2825 2828 2831 2840 2843 3653 4212
Size: 1234 405 3 3 9 3 810 559
Cuts at: 4212 4881 5158 5173 5176 5203 5223 5798 6447
Size: 669 277 15 3 27 20 575 649
Cuts at: 6447 6637 6703 6706 1186
Size: 190 66 3 4231
Fragments arranged by size:
4231 1234 810 669 649 575 559 405
277 190 66 27 20 15 9 3
3 3 3 3
BseYI C'CCAG_C
Cuts at: 3263 3576 5714 8954 9450 3263
Size: 313 2138 3240 496 3564
Fragments arranged by size:
3564 3240 2138 496 313
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3924 4637 5528 5992 6821 1163
Size: 573 2188 713 891 464 829 4093
Fragments arranged by size:
4093 2188 891 829 713 573 464
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7201 7962 8990 1540
Size: 275 186 5200 761 1028 2301
Fragments arranged by size:
5200 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2412 2498 5663 6231 7812 7897 8968 2412
Size: 86 3165 568 1581 85 1071 3195
Fragments arranged by size:
3195 3165 1581 1071 568 86 85
BsmI GAATG_Cn'
Cuts at: 7262 7355 7262
Size: 93 9658
BsmBI CGTCTCn'nnnn_
Cuts at: 3493 3493
Size: 9751
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2412 2498 3576 3935 4394 4742 4796
Size: 399 622 86 1078 359 459 348 54
Cuts at: 4796 5121 5352 5663 6231 6613 7070 7812 7897
Size: 325 231 311 568 382 457 742 85
Cuts at: 7897 8968 1391
Size: 1071 2174
Fragments arranged by size:
2174 1078 1071 742 622 568 459 457
399 382 359 348 325 311 231 86
85 54
BspEI T'CCGG_A
Cuts at: 2083 9414 2083
Size: 7331 2420
BspHI T'CATG_A
Cuts at: 3375 7640 9370 3375
Size: 4265 1730 3756
Fragments arranged by size:
4265 3756 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3028 3946 4667 4858 6517 7106 844
Size: 2184 918 721 191 1659 589 3489
Fragments arranged by size:
3489 2184 1659 918 721 589 191
BsrBI CCG'CTC
Cuts at: 1394 1823 3232 6934 7638 1394
Size: 429 1409 3702 704 3507
Fragments arranged by size:
3702 3507 1409 704 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 8246 8420 65
Size: 1183 6998 174 1396
Fragments arranged by size:
6998 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2674 3216 6909 8395 8582 1700
Size: 110 864 542 3693 1486 187 2869
Fragments arranged by size:
3693 2869 1486 864 542 187 110
BsrGI T'GTAC_A
Cuts at: 96 3663 96
Size: 3567 6184
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9751
BssSI C'ACGA_G
Cuts at: 5669 7811 8823 5669
Size: 2142 1012 6597
Fragments arranged by size:
6597 2142 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2729 4458 5746 6583 6977 794
Size: 1935 1729 1288 837 394 3568
Fragments arranged by size:
3568 1935 1729 1288 837 394
BstBI TT'CG_AA
Cuts at: 8555 8555
Size: 9751
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9751
BstXI CCAn_nnnn'nTGG
Cuts at: 4533 4533
Size: 9751
BstYI r'GATC_y
Cuts at: 1972 2201 3289 3871 4549 4774 4909 5446 5638
Size: 229 1088 582 678 225 135 537 192
Cuts at: 5638 5710 5755 5854 7081 7448 7833 7850 9302
Size: 72 45 99 1227 367 385 17 1452
Cuts at: 9302 9388 9400 9746 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1227 1088 678 582 537 385
367 346 229 225 192 135 99 86
72 45 17 12
BstZ17I GTA'TAC
Cuts at: 2224 2224
Size: 9751
BtgI C'CryG_G
Cuts at: 513 1065 3978 5112 5934 513
Size: 552 2913 1134 822 4330
Fragments arranged by size:
4330 2913 1134 822 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5528 487
Size: 683 56 4302 4710
Fragments arranged by size:
4710 4302 683 56
BtsI GCAGTG_nn'
Cuts at: 3941 4038 4459 7267 8061 8081 3941
Size: 97 421 2808 794 20 5611
Fragments arranged by size:
5611 2808 794 421 97 20
ClaI AT'CG_AT
Cuts at: 1554 4701 1554
Size: 3147 6604
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3169 3204 529
Size: 35 2605 35 7076
Fragments arranged by size:
7076 2605 35 35
DraI TTT'AAA
Cuts at: 2157 2385 5899 7055 2157
Size: 228 3514 1156 4853
Fragments arranged by size:
4853 3514 1156 228
DraIII CAC_nnn'GTG
Cuts at: 6015 6015
Size: 9751
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3980 8758 809
Size: 507 2664 4778 1802
Fragments arranged by size:
4778 2664 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2034 2676 2694
Size: 54 1425 50 275 18 204 642 18
Cuts at: 2694 6747 6789 7198 8087 9716 8
Size: 4053 42 409 889 1629 43
Fragments arranged by size:
4053 1629 1425 889 642 409 275 204
54 50 43 42 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 7198 1537
Size: 275 5386 4090
Fragments arranged by size:
5386 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2325 2460 2637 2802 2817 3042 3420 3564
Size: 972 135 177 165 15 225 378 144
Cuts at: 3564 4921 4992 5005 5176 5194 5221 5340 5775
Size: 1357 71 13 171 18 27 119 435
Cuts at: 5775 5799 6083 7681 1353
Size: 24 284 1598 3423
Fragments arranged by size:
3423 1598 1357 972 435 378 284 225
177 171 165 144 135 119 71 27
24 18 15 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4225 4947 8338 8712 8858 387
Size: 1064 138 2636 722 3391 374 146 1280
Fragments arranged by size:
3391 2636 1280 1064 722 374 146 138
EcoICRI GAG'CTC
Cuts at: 2410 2496 6229 2410
Size: 86 3733 5932
Fragments arranged by size:
5932 3733 86
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2594 2637 2669 2697 2967 3333 3567
Size: 393 962 43 32 28 270 366 234
Cuts at: 3567 3798 4509 4605 5297 5496 5823 6290 6415
Size: 231 711 96 692 199 327 467 125
Cuts at: 6415 6640 6715 6947 7021 7049 7814 8411 9198
Size: 225 75 232 74 28 765 597 787
Cuts at: 9198 9466 1239
Size: 268 1524
Fragments arranged by size:
1524 962 787 765 711 692 597 467
393 366 327 270 268 234 232 231
225 199 125 96 75 74 43 32
28 28
EcoNI CCTnn'n_nnAGG
Cuts at: 2051 2687 4195 6721 2051
Size: 636 1508 2526 5081
Fragments arranged by size:
5081 2526 1508 636
EcoO109I rG'GnC_Cy
Cuts at: 2627 3856 4148 5694 6623 2627
Size: 1229 292 1546 929 5755
Fragments arranged by size:
5755 1546 1229 929 292
EcoRI G'AATT_C
Cuts at: 3379 4595 7060 7438 3379
Size: 1216 2465 378 5692
Fragments arranged by size:
5692 2465 1216 378
EcoRV GAT'ATC
Cuts at: 5298 5298
Size: 9751
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2114 3011 3581 6320 6355 6656 2114
Size: 897 570 2739 35 301 5209
Fragments arranged by size:
5209 2739 897 570 301 35
FspI TGC'GCA
Cuts at: 8257 8257
Size: 9751
HaeII r_GCGC'y
Cuts at: 1678 2481 3238 7017 8898 1678
Size: 803 757 3779 1881 2531
Fragments arranged by size:
3779 2531 1881 803 757
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2582 2614 3490 3522 3755
Size: 32 135 32 1010 32 876 32 233
Cuts at: 3755 3787 4019 4051 4204 4236 4729 4761 5339
Size: 32 232 32 153 32 493 32 578
Cuts at: 5339 5371 5839 5859 5871 5891 8392 8424 8466
Size: 32 468 20 12 20 2501 32 42
Cuts at: 8466 8498 1373
Size: 32 2626
Fragments arranged by size:
2626 2501 1010 876 578 493 468 233
232 153 135 42 32 32 32 32
32 32 32 32 32 32 32 20
20 12
HincII GTy'rAC
Cuts at: 669 1657 1938 2116 3092 6031 7095 7341 669
Size: 988 281 178 976 2939 1064 246 3079
Fragments arranged by size:
3079 2939 1064 988 976 281 246 178
HindIII A'AGCT_T
Cuts at: 748 5893 748
Size: 5145 4606
HpaI GTT'AAC
Cuts at: 7341 7341
Size: 9751
KasI G'GCGC_C
Cuts at: 3234 3234
Size: 9751
KpnI G_GTAC'C
Cuts at: 2060 7076 2060
Size: 5016 4735
MfeI C'AATT_G
Cuts at: 7350 7350
Size: 9751
MluI A'CGCG_T
Cuts at: 9627 9627
Size: 9751
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2850 5174 6211 8865 9049 1305
Size: 1545 2324 1037 2654 184 2007
Fragments arranged by size:
2654 2324 2007 1545 1037 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2696 6749 6791 10
Size: 54 1425 343 864 4053 42 2970
Fragments arranged by size:
4053 2970 1425 864 343 54 42
MslI CAynn'nnrTG
Cuts at: 518 1166 4351 4575 4661 5020 7709 8068 8227
Size: 648 3185 224 86 359 2689 359 159
Cuts at: 8227 518
Size: 2042
Fragments arranged by size:
3185 2689 2042 648 359 359 224 159
86
MspA1I CmG'CkG
Cuts at: 1606 2453 4488 4885 6435 6557 6967 6974 7167
Size: 847 2035 397 1550 122 410 7 193
Cuts at: 7167 7844 8992 9237 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 2035 1550 1148 847 677 410 397
245 193 122 7
NaeI GCC'GGC
Cuts at: 2676 2676
Size: 9751
NarI GG'CG_CC
Cuts at: 3235 3235
Size: 9751
NcoI C'CATG_G
Cuts at: 513 1065 3978 5934 513
Size: 552 2913 1956 4330
Fragments arranged by size:
4330 2913 1956 552
NdeI CA'TA_TG
Cuts at: 387 5736 387
Size: 5349 4402
NgoMIV G'CCGG_C
Cuts at: 2674 2674
Size: 9751
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9751
NotI GC'GGCC_GC
Cuts at: 7198 7198
Size: 9751
NsiI A_TGCA'T
Cuts at: 3144 6268 3144
Size: 3124 6627
NspI r_CATG'y
Cuts at: 1132 1229 2069 2230 2902 3142 4666 6586 7109
Size: 97 840 161 672 240 1524 1920 523
Cuts at: 7109 8654 1132
Size: 1545 2229
Fragments arranged by size:
2229 1920 1545 1524 840 672 523 240
161 97
PasI CC'CwG_GG
Cuts at: 1348 5340 1348
Size: 3992 5759
PciI A'CATG_T
Cuts at: 2065 2226 8650 2065
Size: 161 6424 3166
Fragments arranged by size:
6424 3166 161
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2183 2692 4277 1108
Size: 1075 509 1585 6582
Fragments arranged by size:
6582 1585 1075 509
PfoI T'CCnGG_A
Cuts at: 2685 2685
Size: 9751
PmeI GTTT'AAAC
Cuts at: 7055 7055
Size: 9751
PmlI CAC'GTG
Cuts at: 6841 6841
Size: 9751
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7820 7852 9333 9365 7820
Size: 32 1481 32 8206
Fragments arranged by size:
8206 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2627 3856 4148 2627
Size: 1229 292 8230
Fragments arranged by size:
8230 1229 292
PshAI GACnn'nnGTC
Cuts at: 1613 4526 4785 1613
Size: 2913 259 6579
Fragments arranged by size:
6579 2913 259
PsiI TTA'TAA
Cuts at: 7321 7321
Size: 9751
PstI C_TGCA'G
Cuts at: 830 2623 4117 4671 4726 4860 7103 830
Size: 1793 1494 554 55 134 2243 3478
Fragments arranged by size:
3478 2243 1793 1494 554 134 55
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9751
PvuII CAG'CTG
Cuts at: 2453 4885 6435 6967 6974 2453
Size: 2432 1550 532 7 5230
Fragments arranged by size:
5230 2432 1550 532 7
RsrII CG'GwC_CG
Cuts at: 4787 4787
Size: 9751
SacI G_AGCT'C
Cuts at: 2412 2498 6231 2412
Size: 86 3733 5932
Fragments arranged by size:
5932 3733 86
SalI G'TCGA_C
Cuts at: 1936 6029 7093 1936
Size: 4093 1064 4594
Fragments arranged by size:
4594 4093 1064
SapI GCTCTTCn'nnn_
Cuts at: 2325 2460 3564 5340 5799 2325
Size: 135 1104 1776 459 6277
Fragments arranged by size:
6277 1776 1104 459 135
SbfI CC_TGCA'GG
Cuts at: 4117 7103 4117
Size: 2986 6765
ScaI AGT'ACT
Cuts at: 3185 7999 9711 3185
Size: 4814 1712 3225
Fragments arranged by size:
4814 3225 1712
SfcI C'TryA_G
Cuts at: 826 1045 2619 3275 4113 4667 4710 4722 4856
Size: 219 1574 656 838 554 43 12 134
Cuts at: 4856 7099 8234 8915 9106 826
Size: 2243 1135 681 191 1471
Fragments arranged by size:
2243 1574 1471 1135 838 681 656 554
219 191 134 43 12
SfoI GGC'GCC
Cuts at: 3236 3236
Size: 9751
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9751
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9751
SmaI CCC'GGG
Cuts at: 1872 5143 7078 1872
Size: 3271 1935 4545
Fragments arranged by size:
4545 3271 1935
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2666 3128 4192 6985 7855 8756
Size: 197 926 723 462 1064 2793 870 901
Cuts at: 8756 9018 820
Size: 262 1553
Fragments arranged by size:
2793 1553 1064 926 901 870 723 462
262 197
SnaBI TAC'GTA
Cuts at: 493 7542 493
Size: 7049 2702
SpeI A'CTAG_T
Cuts at: 152 3743 152
Size: 3591 6160
SphI G_CATG'C
Cuts at: 1132 2902 4666 6586 7109 1132
Size: 1770 1764 1920 523 3774
Fragments arranged by size:
3774 1920 1770 1764 523
SspI AAT'ATT
Cuts at: 5 52 3464 5977 7675 5
Size: 47 3412 2513 1698 2081
Fragments arranged by size:
3412 2513 2081 1698 47
StuI AGG'CCT
Cuts at: 1517 3564 1517
Size: 2047 7704
StyI C'CwwG_G
Cuts at: 513 1065 3978 5934 6885 7190 513
Size: 552 2913 1956 951 305 3074
Fragments arranged by size:
3074 2913 1956 951 552 305
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3620 4310 4775 5034 5661 6644 7778
Size: 1255 2040 690 465 259 627 983 1134
Cuts at: 7778 7795 7948 8133 8578 325
Size: 17 153 185 445 1498
Fragments arranged by size:
2040 1498 1255 1134 983 690 627 465
445 259 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3183 3663 6289
Size: 274 80 33 51 1443 1206 480 2626
Cuts at: 6289 7997 9709 96
Size: 1708 1712 138
Fragments arranged by size:
2626 1712 1708 1443 1206 480 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 9711 9743 9711
Size: 32 9719
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9751
XbaI T'CTAG_A
Cuts at: 6926 7087 6926
Size: 161 9590
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2044 2253 5110 6148 2044
Size: 209 2857 1038 5647
Fragments arranged by size:
5647 2857 1038 209
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9751
XmaI C'CCGG_G
Cuts at: 1870 5141 7076 1870
Size: 3271 1935 4545
Fragments arranged by size:
4545 3271 1935
XmnI GAAnn'nnTTC
Cuts at: 3873 7034 7880 3873
Size: 3161 846 5744
Fragments arranged by size:
5744 3161 846
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7559 276
Size: 53 83 186 936 6025 2468
Fragments arranged by size:
6025 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BsiWI Bsu36I FseI
FspAI NruI PacI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SrfI SwaI