Restriction map for
pFN21AE5827
(Circular) (Six-base) MAPSORT of: pFN21AE5827.seq Check: 7875 from: 1 to: 7456
pFN21AE5827 7456 bp
With 182 enzymes: SgfI *
December 8, 2014 13:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3709 3709
Size: 7456
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2988 5266 278
Size: 53 83 186 936 1452 2278 2468
Fragments arranged by size:
2468 2278 1452 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2345 4799 1937
Size: 408 2454 4594
Fragments arranged by size:
4594 2454 408
Acc65I G'GTAC_C
Cuts at: 4777 4777
Size: 7456
AclI AA'CG_TT
Cuts at: 1563 5583 5956 1563
Size: 4020 373 3063
Fragments arranged by size:
4020 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3021 3429 4258 5519 6903 7171 1632
Size: 1389 408 829 1261 1384 268 1917
Fragments arranged by size:
1917 1389 1384 1261 829 408 268
AfeI AGC'GCT
Cuts at: 2483 2483
Size: 7456
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7259
AflIII A'CryG_T
Cuts at: 1203 6355 7332 1203
Size: 5152 977 1327
Fragments arranged by size:
5152 1327 977
AgeI A'CCGG_T
Cuts at: 6287 6287
Size: 7456
AhdI GACnn_n'nnGTC
Cuts at: 2359 6185 2359
Size: 3826 3630
AleI CACnn'nnGTG
Cuts at: 1166 2492 1166
Size: 1326 6130
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3467 4019 1184
Size: 2283 552 4621
Fragments arranged by size:
4621 2283 552
AlwNI CAG_nnn'CTG
Cuts at: 2173 3689 5199 6771 2173
Size: 1516 1510 1572 2858
Fragments arranged by size:
2858 1572 1516 1510
ApaI G_GGCC'C
Cuts at: 4023 4023
Size: 7456
ApaLI G'TGCA_C
Cuts at: 5513 6669 5513
Size: 1156 6300
ApoI r'AATT_y
Cuts at: 1493 3050 4765 4991 5143 7252 1493
Size: 1557 1715 226 152 2109 1697
Fragments arranged by size:
2109 1715 1697 1557 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6010 160
Size: 872 4978 1606
Fragments arranged by size:
4978 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7456
AvaI C'yCGr_G
Cuts at: 1870 1943 2068 3585 3787 4014 4781 1870
Size: 73 125 1517 202 227 767 4545
Fragments arranged by size:
4545 1517 767 227 202 125 73
BamHI G'GATC_C
Cuts at: 3413 4786 5153 3413
Size: 1373 367 5716
Fragments arranged by size:
5716 1373 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2783 3138 3260 3975 4067
Size: 325 217 627 996 355 122 715 92
Cuts at: 4067 4777 6232 618
Size: 710 1455 1842
Fragments arranged by size:
1842 1455 996 715 710 627 355 325
217 122 92
BanII G_rGCy'C
Cuts at: 1391 2025 3291 3668 4014 4023 4303 4671 4775
Size: 634 1266 377 346 9 280 368 104
Cuts at: 4775 1391
Size: 4072
Fragments arranged by size:
4072 1266 634 377 368 346 280 104
9
BbeI G_GCGC'C
Cuts at: 3979 4071 3979
Size: 92 7364
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2708 3048 928
Size: 971 809 340 5336
Fragments arranged by size:
5336 971 809 340
BbvCI CC'TCA_GC
Cuts at: 3936 3936
Size: 7456
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3898 3932 4005 4039 5647 5681 1903
Size: 34 1961 34 73 34 1608 34 3678
Fragments arranged by size:
3678 1961 1608 73 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2617 5348 6558 2617
Size: 2731 1210 3515
Fragments arranged by size:
3515 2731 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7456
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4018 6067 136
Size: 107 122 71 631 605 2346 2049 1525
Fragments arranged by size:
2346 2049 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2842 3607 4533 7451 2842
Size: 765 926 2918 2847
Fragments arranged by size:
2918 2847 926 765
BlpI GC'TnA_GC
Cuts at: 4873 4873
Size: 7456
Bme1580I G_kGCm'C
Cuts at: 1790 4023 5517 6673 1790
Size: 2233 1494 1156 2573
Fragments arranged by size:
2573 2233 1494 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7456
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2250 2646 3699
Size: 416 53 83 399 24 833 396 1053
Cuts at: 3699 5187 6145 442
Size: 1488 958 1753
Fragments arranged by size:
1753 1488 1053 958 833 416 399 396
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7456
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2097 2342 2993 3297 3405 3459 4317 4481
Size: 858 245 651 304 108 54 858 164
Cuts at: 4481 6116 1239
Size: 1635 2579
Fragments arranged by size:
2579 1635 858 858 651 304 245 164
108 54
Bpu10I CC'TnA_GC
Cuts at: 2415 3936 2415
Size: 1521 5935
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2758 4091 4349 5581 6446 6744 2758
Size: 1333 258 1232 865 298 3470
Fragments arranged by size:
3470 1333 1232 865 298 258
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3631 6119 882
Size: 617 2132 2488 2219
Fragments arranged by size:
2488 2219 2132 617
BsaAI yAC'GTr
Cuts at: 493 1204 5247 493
Size: 711 4043 2702
Fragments arranged by size:
4043 2702 711
BsaBI GATnn'nnATC
Cuts at: 2063 7450 2063
Size: 5387 2069
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2985 3976 4068 5263
Size: 53 83 186 936 1452 991 92 1195
Cuts at: 5263 5645 6280 275
Size: 382 635 1451
Fragments arranged by size:
1452 1451 1195 991 936 635 382 186
92 83 53
BsaWI w'CCGG_w
Cuts at: 2244 5889 6287 6561 6708 7119 2244
Size: 3645 398 274 147 411 2581
Fragments arranged by size:
3645 2581 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2340 2370 2638 2668 4199
Size: 30 165 30 937 30 268 30 1531
Cuts at: 4199 4229 4454 4484 4568 4598 1178
Size: 30 225 30 84 30 4036
Fragments arranged by size:
4036 1531 937 268 225 165 84 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2894 3812 3833 3842 3860 4466 1186
Size: 1708 918 21 9 18 606 4176
Fragments arranged by size:
4176 1708 918 606 21 18 9
BseYI C'CCAG_C
Cuts at: 2475 3278 3332 3368 3440 4097 4117 4264 6659
Size: 803 54 36 72 657 20 147 2395
Cuts at: 6659 7155 2475
Size: 496 2776
Fragments arranged by size:
2776 2395 803 657 496 147 72 54
36 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2374 2511 2729 3980 1163
Size: 573 638 137 218 1251 4639
Fragments arranged by size:
4639 1251 638 573 218 137
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4906 5667 6695 1540
Size: 275 186 2905 761 1028 2301
Fragments arranged by size:
2905 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2039 2214 3063 3668 4602 5517 5602 6673 2039
Size: 175 849 605 934 915 85 1071 2822
Fragments arranged by size:
2822 1071 934 915 849 605 175 85
BsmI GAATG_Cn'
Cuts at: 4967 5060 4967
Size: 93 7363
BsmBI CGTCTCn'nnnn_
Cuts at: 3200 3914 3200
Size: 714 6742
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2025 2039 2214 3063 3291 3668 4014
Size: 399 235 14 175 849 228 377 346
Cuts at: 4014 4023 4303 4602 4671 4775 5517 5602 6673
Size: 9 280 299 69 104 742 85 1071
Cuts at: 6673 1391
Size: 2174
Fragments arranged by size:
2174 1071 849 742 399 377 346 299
280 235 228 175 104 85 69 14
9
BspEI T'CCGG_A
Cuts at: 2244 7119 2244
Size: 4875 2581
BspHI T'CATG_A
Cuts at: 5345 7075 5345
Size: 1730 5726
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2952 3517 3709 4623 4811 844
Size: 2108 565 192 914 188 3489
Fragments arranged by size:
3489 2108 914 565 192 188
BsrBI CCG'CTC
Cuts at: 1394 1823 3365 4182 5343 1394
Size: 429 1542 817 1161 3507
Fragments arranged by size:
3507 1542 1161 817 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4132 5951 6125 65
Size: 1183 2884 1819 174 1396
Fragments arranged by size:
2884 1819 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6100 6287 1700
Size: 110 4290 187 2869
Fragments arranged by size:
4290 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7456
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7456
BssSI C'ACGA_G
Cuts at: 2237 2273 5516 6528 2237
Size: 36 3243 1012 3165
Fragments arranged by size:
3243 3165 1012 36
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2034 2521 794
Size: 1240 487 5729
Fragments arranged by size:
5729 1240 487
BstBI TT'CG_AA
Cuts at: 6260 6260
Size: 7456
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7456
BstXI CCAn_nnnn'nTGG
Cuts at: 2812 2812
Size: 7456
BstYI r'GATC_y
Cuts at: 1972 2241 2842 3413 3607 4269 4533 4786 5153
Size: 269 601 571 194 662 264 253 367
Cuts at: 5153 5538 5555 7007 7093 7105 7451 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 662 601 571 385 367 346
269 264 253 194 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2990 3732 2990
Size: 742 6714
BtgI C'CryG_G
Cuts at: 513 1065 2130 2395 2725 4308 513
Size: 552 1065 265 330 1583 3661
Fragments arranged by size:
3661 1583 1065 552 330 265
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3139 3885 4534 487
Size: 683 56 1913 746 649 3409
Fragments arranged by size:
3409 1913 746 683 649 56
BtsI GCAGTG_nn'
Cuts at: 2541 3898 3950 4048 4422 4972 5766 5786 2541
Size: 1357 52 98 374 550 794 20 4211
Fragments arranged by size:
4211 1357 794 550 374 98 52 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7456
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7421
DraI TTT'AAA
Cuts at: 4760 4760
Size: 7456
DraIII CAC_nnn'GTG
Cuts at: 3956 3956
Size: 7456
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6463 809
Size: 507 5147 1802
Fragments arranged by size:
5147 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2496 2974 3298
Size: 54 1425 50 275 18 666 478 324
Cuts at: 3298 4903 5792 7421 8
Size: 1605 889 1629 43
Fragments arranged by size:
1629 1605 1425 889 666 478 324 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4903 1537
Size: 275 3091 4090
Fragments arranged by size:
4090 3091 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2957 3119 3218 3804 4160 4217 5386 1353
Size: 1604 162 99 586 356 57 1169 3423
Fragments arranged by size:
3423 1604 1169 586 356 162 99 57
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2165 3426 4297 6043 6417 6563
Size: 1064 138 576 1261 871 1746 374 146
Cuts at: 6563 387
Size: 1280
Fragments arranged by size:
1746 1280 1261 1064 871 576 374 146
138
EcoICRI GAG'CTC
Cuts at: 3666 3666
Size: 7456
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2097 2342 2993 3021 3297 3405 3429
Size: 393 465 245 651 28 276 108 24
Cuts at: 3429 3459 4258 4317 4481 5519 6116 6903 7171
Size: 30 799 59 164 1038 597 787 268
Cuts at: 7171 1239
Size: 1524
Fragments arranged by size:
1524 1038 799 787 651 597 465 393
276 268 245 164 108 59 30 28
24
EcoNI CCTnn'n_nnAGG
Cuts at: 3650 4004 4244 3650
Size: 354 240 6862
Fragments arranged by size:
6862 354 240
EcoO109I rG'GnC_Cy
Cuts at: 2320 3254 3502 3538 3589 3643 3673 3694 4019
Size: 934 248 36 51 54 30 21 325
Cuts at: 4019 4020 2320
Size: 1 5756
Fragments arranged by size:
5756 934 325 248 54 51 36 30
21 1
EcoRI G'AATT_C
Cuts at: 4765 5143 4765
Size: 378 7078
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2101 4448 4552 4678 2101
Size: 2347 104 126 4879
Fragments arranged by size:
4879 2347 126 104
FspI TGC'GCA
Cuts at: 5962 5962
Size: 7456
HaeII r_GCGC'y
Cuts at: 1678 2047 2425 2485 3979 4071 6603 1678
Size: 369 378 60 1494 92 2532 2531
Fragments arranged by size:
2532 2531 1494 378 369 92 60
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2345 2377 4199 4231 6097
Size: 32 135 32 773 32 1822 32 1866
Cuts at: 6097 6129 6171 6203 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1866 1822 773 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2346 4576 4800 5046 669
Size: 988 281 408 2230 224 246 3079
Fragments arranged by size:
3079 2230 988 408 281 246 224
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7456
HpaI GTT'AAC
Cuts at: 5046 5046
Size: 7456
KasI G'GCGC_C
Cuts at: 3975 4067 3975
Size: 92 7364
KpnI G_GTAC'C
Cuts at: 4781 4781
Size: 7456
MfeI C'AATT_G
Cuts at: 5055 5055
Size: 7456
MluI A'CGCG_T
Cuts at: 7332 7332
Size: 7456
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3126 4059 6570 6754 1305
Size: 1821 933 2511 184 2007
Fragments arranged by size:
2511 2007 1821 933 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2498 3300 10
Size: 54 1425 343 666 802 4166
Fragments arranged by size:
4166 1425 802 666 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2492 5414 5773 5932 518
Size: 648 1326 2922 359 159 2042
Fragments arranged by size:
2922 2042 1326 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2173 2191 2430 2581 2599 2979 3172 4310
Size: 567 18 239 151 18 380 193 1138
Cuts at: 4310 4872 5549 6697 6942 1606
Size: 562 677 1148 245 2120
Fragments arranged by size:
2120 1148 1138 677 567 562 380 245
239 193 151 18 18
NarI GG'CG_CC
Cuts at: 3976 4068 3976
Size: 92 7364
NcoI C'CATG_G
Cuts at: 513 1065 2395 513
Size: 552 1330 5574
Fragments arranged by size:
5574 1330 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7456
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7456
NotI GC'GGCC_GC
Cuts at: 4903 4903
Size: 7456
NruI TCG'CGA
Cuts at: 4553 4553
Size: 7456
NspI r_CATG'y
Cuts at: 1132 1229 2765 4090 4566 4814 6359 1132
Size: 97 1536 1325 476 248 1545 2229
Fragments arranged by size:
2229 1545 1536 1325 476 248 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7456
PciI A'CATG_T
Cuts at: 6355 6355
Size: 7456
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2131 1108
Size: 1023 6433
PfoI T'CCnGG_A
Cuts at: 3313 3570 4217 3313
Size: 257 647 6552
Fragments arranged by size:
6552 647 257
PmeI GTTT'AAAC
Cuts at: 4760 4760
Size: 7456
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5525 5557 7038 7070 5525
Size: 32 1481 32 5911
Fragments arranged by size:
5911 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2320 3502 3538 3589 3673 3694 2320
Size: 1182 36 51 84 21 6082
Fragments arranged by size:
6082 1182 84 51 36 21
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7456
PsiI TTA'TAA
Cuts at: 5026 5026
Size: 7456
PspOMI G'GGCC_C
Cuts at: 4019 4019
Size: 7456
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2987 3019 2987
Size: 32 7424
PstI C_TGCA'G
Cuts at: 830 2169 2190 3027 4008 4620 4808 830
Size: 1339 21 837 981 612 188 3478
Fragments arranged by size:
3478 1339 981 837 612 188 21
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7456
PvuII CAG'CTG
Cuts at: 2173 2191 2430 2599 3172 2173
Size: 18 239 169 573 6457
Fragments arranged by size:
6457 573 239 169 18
SacI G_AGCT'C
Cuts at: 3668 3668
Size: 7456
SacII CC_GC'GG
Cuts at: 4311 4311
Size: 7456
SalI G'TCGA_C
Cuts at: 1936 2344 4798 1936
Size: 408 2454 4594
Fragments arranged by size:
4594 2454 408
SanDI GG'GwC_CC
Cuts at: 3694 3694
Size: 7456
SapI GCTCTTCn'nnn_
Cuts at: 2957 2957
Size: 7456
SbfI CC_TGCA'GG
Cuts at: 4808 4808
Size: 7456
ScaI AGT'ACT
Cuts at: 5704 7416 5704
Size: 1712 5744
SexAI A'CCwGG_T
Cuts at: 2436 2436
Size: 7456
SfcI C'TryA_G
Cuts at: 826 1045 2165 2186 3023 3754 4004 4616 4804
Size: 219 1120 21 837 731 250 612 188
Cuts at: 4804 5939 6620 6811 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1120 837 731 681 612 250
219 191 188 21
SfoI GGC'GCC
Cuts at: 3977 4069 3977
Size: 92 7364
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7456
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7456
SmaI CCC'GGG
Cuts at: 1872 3587 4783 1872
Size: 1715 1196 4545
Fragments arranged by size:
4545 1715 1196
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2773 4106 4364 5560 6461 6723
Size: 197 926 830 1333 258 1196 901 262
Cuts at: 6723 820
Size: 1553
Fragments arranged by size:
1553 1333 1196 926 901 830 262 258
197
SnaBI TAC'GTA
Cuts at: 493 5247 493
Size: 4754 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7456
SphI G_CATG'C
Cuts at: 1132 2765 4090 4566 4814 1132
Size: 1633 1325 476 248 3774
Fragments arranged by size:
3774 1633 1325 476 248
SspI AAT'ATT
Cuts at: 5 52 5380 5
Size: 47 5328 2081
Fragments arranged by size:
5328 2081 47
StuI AGG'CCT
Cuts at: 1517 2203 2676 1517
Size: 686 473 6297
Fragments arranged by size:
6297 686 473
StyI C'CwwG_G
Cuts at: 513 1065 2395 2958 3237 3639 4245 4380 4521
Size: 552 1330 563 279 402 606 135 141
Cuts at: 4521 4605 4895 513
Size: 84 290 3074
Fragments arranged by size:
3074 1330 606 563 552 402 290 279
141 135 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2408 3292 3346 5483 5500 5653 5838
Size: 1255 828 884 54 2137 17 153 185
Cuts at: 5838 6283 325
Size: 445 1498
Fragments arranged by size:
2137 1498 1255 884 828 445 185 153
54 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5702 7414 96
Size: 274 80 33 51 1443 3725 1712 138
Fragments arranged by size:
3725 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2340 2372 4133 4165 7416 7448 2340
Size: 32 1761 32 3251 32 2348
Fragments arranged by size:
3251 2348 1761 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7456
XbaI T'CTAG_A
Cuts at: 2839 4792 2839
Size: 1953 5503
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7456
XmaI C'CCGG_G
Cuts at: 1870 3585 4781 1870
Size: 1715 1196 4545
Fragments arranged by size:
4545 1715 1196
XmnI GAAnn'nnTTC
Cuts at: 2012 4337 5585 2012
Size: 2325 1248 3883
Fragments arranged by size:
3883 2325 1248
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2986 5264 276
Size: 53 83 186 936 1452 2278 2468
Fragments arranged by size:
2468 2278 1452 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NruI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI
PvuII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I
EcoRV FseI FspAI NaeI NgoMIV NsiI PacI PmlI
PspXI RsrII SfiI SrfI SwaI XcmI