Restriction map for
pFN21AE5825
(Circular) (Six-base) MAPSORT of: pFN21AE5825.seq Check: 2115 from: 1 to: 8353
pFN21AE5825 8353 bp
With 182 enzymes: SgfI *
December 8, 2014 13:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2022 2022
Size: 8353
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2151 6163 278
Size: 53 83 186 936 615 4012 2468
Fragments arranged by size:
4012 2468 936 615 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5239 5696 1937
Size: 3302 457 4594
Fragments arranged by size:
4594 3302 457
Acc65I G'GTAC_C
Cuts at: 5674 5674
Size: 8353
AclI AA'CG_TT
Cuts at: 1563 2448 6480 6853 1563
Size: 885 4032 373 3063
Fragments arranged by size:
4032 3063 885 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2127 3188 3261 3549 4200 4289 4808 4915
Size: 495 1061 73 288 651 89 519 107
Cuts at: 4915 6416 7800 8068 1632
Size: 1501 1384 268 1917
Fragments arranged by size:
1917 1501 1384 1061 651 519 495 288
268 107 89 73
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8156
AflIII A'CryG_T
Cuts at: 1203 7252 8229 1203
Size: 6049 977 1327
Fragments arranged by size:
6049 1327 977
AgeI A'CCGG_T
Cuts at: 7184 7184
Size: 8353
AhdI GACnn_n'nnGTC
Cuts at: 7082 7082
Size: 8353
AleI CACnn'nnGTG
Cuts at: 1166 2459 3321 3395 3467 3920 5412 1166
Size: 1293 862 74 72 453 1492 4107
Fragments arranged by size:
4107 1492 1293 862 453 74 72
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2679 1184
Size: 1495 6858
AlwNI CAG_nnn'CTG
Cuts at: 2165 2924 3572 3761 4115 4319 6096 7668 2165
Size: 759 648 189 354 204 1777 1572 2850
Fragments arranged by size:
2850 1777 1572 759 648 354 204 189
ApaLI G'TGCA_C
Cuts at: 6410 7566 6410
Size: 1156 7197
ApoI r'AATT_y
Cuts at: 1493 2213 2222 2823 2939 3152 4814 5662 5888
Size: 720 9 601 116 213 1662 848 226
Cuts at: 5888 6040 8149 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1662 848 720 601 226 213
152 116 9
AseI AT'TA_AT
Cuts at: 160 1032 4707 6907 160
Size: 872 3675 2200 1606
Fragments arranged by size:
3675 2200 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8353
AvaI C'yCGr_G
Cuts at: 1870 1943 2830 5678 1870
Size: 73 887 2848 4545
Fragments arranged by size:
4545 2848 887 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4569 4602 4569
Size: 33 8320
BamHI G'GATC_C
Cuts at: 5683 6050 5683
Size: 367 7986
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2010 2033 3509 5674 7129
Size: 325 217 627 223 23 1476 2165 1455
Cuts at: 7129 618
Size: 1842
Fragments arranged by size:
2165 1842 1476 1455 627 325 223 217
23
BanII G_rGCy'C
Cuts at: 1391 5672 1391
Size: 4281 4072
BbeI G_GCGC'C
Cuts at: 2037 2037
Size: 8353
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 7382
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5453 5487 6544 6578 1903
Size: 34 3516 34 1057 34 3678
Fragments arranged by size:
3678 3516 1057 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2019 4997 5354 6245 7455 2019
Size: 2978 357 891 1210 2917
Fragments arranged by size:
2978 2917 1210 891 357
BclI T'GATC_A
Cuts at: 1548 5308 5400 1548
Size: 3760 92 4501
Fragments arranged by size:
4501 3760 92
BfrBI ATG'CAT
Cuts at: 5284 5284
Size: 8353
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3902 6964 136
Size: 107 122 71 631 605 2230 3062 1525
Fragments arranged by size:
3062 2230 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4876 8348 4876
Size: 3472 4881
BlpI GC'TnA_GC
Cuts at: 5770 5770
Size: 8353
Bme1580I G_kGCm'C
Cuts at: 1790 2013 3514 5322 6414 7570 1790
Size: 223 1501 1808 1092 1156 2573
Fragments arranged by size:
2573 1808 1501 1156 1092 223
BmgBI CAC'GTC
Cuts at: 1318 3840 1318
Size: 2522 5831
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2485 2731 3508
Size: 416 53 83 399 24 1068 246 777
Cuts at: 3508 3893 4401 5239 6084 7042 442
Size: 385 508 838 845 958 1753
Fragments arranged by size:
1753 1068 958 845 838 777 508 416
399 385 246 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8353
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4071 4270 4330 7013 1239
Size: 2832 199 60 2683 2579
Fragments arranged by size:
2832 2683 2579 199 60
Bpu10I CC'TnA_GC
Cuts at: 4490 4490
Size: 8353
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3460 6478 7343 7641 3460
Size: 3018 865 298 4172
Fragments arranged by size:
4172 3018 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 7016 882
Size: 617 5517 2219
Fragments arranged by size:
5517 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3319 3688 6144 493
Size: 711 2115 369 2456 2702
Fragments arranged by size:
2702 2456 2115 711 369
BsaBI GATnn'nnATC
Cuts at: 8347 8347
Size: 8353
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2034 2148 6160 6542
Size: 53 83 186 936 501 114 4012 382
Cuts at: 6542 7177 275
Size: 635 1451
Fragments arranged by size:
4012 1451 936 635 501 382 186 114
83 53
BsaWI w'CCGG_w
Cuts at: 2493 2846 3907 5247 5526 6786 7184 7458 7605
Size: 353 1061 1340 279 1260 398 274 147
Cuts at: 7605 8016 2493
Size: 411 2830
Fragments arranged by size:
2830 1340 1260 1061 411 398 353 279
274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2224 2254 1178
Size: 30 165 30 821 30 7277
Fragments arranged by size:
7277 821 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2232 4847 5564 1186
Size: 1046 2615 717 3975
Fragments arranged by size:
3975 2615 1046 717
BseYI C'CCAG_C
Cuts at: 7556 8052 7556
Size: 496 7857
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3445 4992 1163
Size: 573 1709 1547 4524
Fragments arranged by size:
4524 1709 1547 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3639 5803 6564 7592 1540
Size: 275 186 1638 2164 761 1028 2301
Fragments arranged by size:
2301 2164 1638 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 6414 6499 7570 6414
Size: 85 1071 7197
Fragments arranged by size:
7197 1071 85
BsmI GAATG_Cn'
Cuts at: 2086 4002 5864 5957 2086
Size: 1916 1862 93 4482
Fragments arranged by size:
4482 1916 1862 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2892 4435 2892
Size: 1543 6810
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 3514 5322 5672 6414 6499 7570
Size: 399 223 1501 1808 350 742 85 1071
Cuts at: 7570 1391
Size: 2174
Fragments arranged by size:
2174 1808 1501 1071 742 399 350 223
85
BspEI T'CCGG_A
Cuts at: 2493 3907 5247 8016 2493
Size: 1414 1340 2769 2830
Fragments arranged by size:
2830 2769 1414 1340
BspHI T'CATG_A
Cuts at: 2760 4062 5479 6242 7972 2760
Size: 1302 1417 763 1730 3141
Fragments arranged by size:
3141 1730 1417 1302 763
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2022 3425 5708 844
Size: 1178 1403 2283 3489
Fragments arranged by size:
3489 2283 1403 1178
BsrBI CCG'CTC
Cuts at: 1394 1823 6240 1394
Size: 429 4417 3507
Fragments arranged by size:
4417 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4063 5442 6848 7022 65
Size: 1183 2815 1379 1406 174 1396
Fragments arranged by size:
2815 1406 1396 1379 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6997 7184 1700
Size: 110 5187 187 2869
Fragments arranged by size:
5187 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 5356 96
Size: 5260 3093
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8353
BssSI C'ACGA_G
Cuts at: 6413 7425 6413
Size: 1012 7341
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5333 794
Size: 4539 3814
BstBI TT'CG_AA
Cuts at: 2211 7157 2211
Size: 4946 3407
BstEII G'GTnAC_C
Cuts at: 1184 2317 3051 3826 1184
Size: 1133 734 775 5711
Fragments arranged by size:
5711 1133 775 734
BstXI CCAn_nnnn'nTGG
Cuts at: 2901 4629 2901
Size: 1728 6625
BstYI r'GATC_y
Cuts at: 1972 3218 3568 3847 4876 5683 6050 6435 6452
Size: 1246 350 279 1029 807 367 385 17
Cuts at: 6452 7904 7990 8002 8348 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1246 1029 807 385 367 350
346 279 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3214 3536 3214
Size: 322 8031
BtgI C'CryG_G
Cuts at: 513 1065 2004 2019 2958 3639 3831 513
Size: 552 939 15 939 681 192 5035
Fragments arranged by size:
5035 939 939 681 552 192 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7614
Fragments arranged by size:
7614 683 56
BtsI GCAGTG_nn'
Cuts at: 2875 3069 3345 4203 4362 5042 5295 5407 5419
Size: 194 276 858 159 680 253 112 12
Cuts at: 5419 5869 6663 6683 2875
Size: 450 794 20 4545
Fragments arranged by size:
4545 858 794 680 450 276 253 194
159 112 20 12
ClaI AT'CG_AT
Cuts at: 1554 4891 1554
Size: 3337 5016
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3058 3093 5570 5605 529
Size: 35 2494 35 2477 35 3277
Fragments arranged by size:
3277 2494 2477 35 35 35
DraI TTT'AAA
Cuts at: 4731 5657 4731
Size: 926 7427
DraIII CAC_nnn'GTG
Cuts at: 2987 5118 2987
Size: 2131 6222
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2998 7360 809
Size: 507 1682 4362 1802
Fragments arranged by size:
4362 1802 1682 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3636 4482 4794
Size: 54 1425 50 275 18 1806 846 312
Cuts at: 4794 5156 5800 6689 8318 8
Size: 362 644 889 1629 43
Fragments arranged by size:
1806 1629 1425 889 846 644 362 312
275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3636 5800 1537
Size: 275 1824 2164 4090
Fragments arranged by size:
4090 2164 1824 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2801 3102 4196 4233 4730 4827 4956 5211
Size: 1448 301 1094 37 497 97 129 255
Cuts at: 5211 6283 1353
Size: 1072 3423
Fragments arranged by size:
3423 1448 1094 1072 497 301 255 129
97 37
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4142 6940 7314 7460 387
Size: 1064 138 2553 2798 374 146 1280
Fragments arranged by size:
2798 2553 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2127 3188 3261 3549 4071 4200 4270
Size: 393 495 1061 73 288 522 129 70
Cuts at: 4270 4289 4330 4808 4915 6416 7013 7800 8068
Size: 19 41 478 107 1501 597 787 268
Cuts at: 8068 1239
Size: 1524
Fragments arranged by size:
1524 1501 1061 787 597 522 495 478
393 288 268 129 107 73 70 41
19
EcoNI CCTnn'n_nnAGG
Cuts at: 2406 3666 4603 2406
Size: 1260 937 6156
Fragments arranged by size:
6156 1260 937
EcoO109I rG'GnC_Cy
Cuts at: 3545 3629 5393 3545
Size: 84 1764 6505
Fragments arranged by size:
6505 1764 84
EcoRI G'AATT_C
Cuts at: 2222 5662 6040 2222
Size: 3440 378 4535
Fragments arranged by size:
4535 3440 378
EcoRV GAT'ATC
Cuts at: 4858 4858
Size: 8353
FspI TGC'GCA
Cuts at: 6859 6859
Size: 8353
HaeII r_GCGC'y
Cuts at: 1678 2037 7500 1678
Size: 359 5463 2531
Fragments arranged by size:
5463 2531 359
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2897 2929 4672 4704 4842
Size: 32 135 32 1325 32 1743 32 138
Cuts at: 4842 4874 5377 5409 6994 7026 7068 7100 1373
Size: 32 503 32 1585 32 42 32 2626
Fragments arranged by size:
2626 1743 1585 1325 503 138 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2349 3003 4274 5491 5697 5943
Size: 988 281 411 654 1271 1217 206 246
Cuts at: 5943 669
Size: 3079
Fragments arranged by size:
3079 1271 1217 988 654 411 281 246
206
HindIII A'AGCT_T
Cuts at: 748 2139 4616 748
Size: 1391 2477 4485
Fragments arranged by size:
4485 2477 1391
HpaI GTT'AAC
Cuts at: 2349 5943 2349
Size: 3594 4759
KasI G'GCGC_C
Cuts at: 2033 2033
Size: 8353
KpnI G_GTAC'C
Cuts at: 5678 5678
Size: 8353
MfeI C'AATT_G
Cuts at: 4372 5952 4372
Size: 1580 6773
MluI A'CGCG_T
Cuts at: 8229 8229
Size: 8353
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3935 5392 7467 7651 1305
Size: 2630 1457 2075 184 2007
Fragments arranged by size:
2630 2075 2007 1457 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4484 4796 5158 10
Size: 54 1425 343 2652 312 362 3205
Fragments arranged by size:
3205 2652 1425 362 343 312 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2459 2899 3321 3395 3467 3730 3920
Size: 648 1293 440 422 74 72 263 190
Cuts at: 3920 5195 5412 5574 6311 6670 6829 518
Size: 1275 217 162 737 359 159 2042
Fragments arranged by size:
2042 1293 1275 737 648 440 422 359
263 217 190 162 159 74 72
MspA1I CmG'CkG
Cuts at: 1606 2584 5769 6446 7594 7839 1606
Size: 978 3185 677 1148 245 2120
Fragments arranged by size:
3185 2120 1148 978 677 245
NarI GG'CG_CC
Cuts at: 2034 2034
Size: 8353
NcoI C'CATG_G
Cuts at: 513 1065 2004 2958 3831 513
Size: 552 939 954 873 5035
Fragments arranged by size:
5035 954 939 873 552
NdeI CA'TA_TG
Cuts at: 387 3961 4924 387
Size: 3574 963 3816
Fragments arranged by size:
3816 3574 963
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8353
NotI GC'GGCC_GC
Cuts at: 5800 5800
Size: 8353
NsiI A_TGCA'T
Cuts at: 5286 5286
Size: 8353
NspI r_CATG'y
Cuts at: 1132 1229 5711 7256 1132
Size: 97 4482 1545 2229
Fragments arranged by size:
4482 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 5035 1348
Size: 3687 4666
PciI A'CATG_T
Cuts at: 7252 7252
Size: 8353
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8353
PfoI T'CCnGG_A
Cuts at: 3359 4999 3359
Size: 1640 6713
PmeI GTTT'AAAC
Cuts at: 5657 5657
Size: 8353
PmlI CAC'GTG
Cuts at: 3319 3319
Size: 8353
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6422 6454 7935 7967 6422
Size: 32 1481 32 6808
Fragments arranged by size:
6808 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3629 5393 3629
Size: 1764 6589
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8353
PsiI TTA'TAA
Cuts at: 5923 5923
Size: 8353
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2456 2488 2456
Size: 32 8321
PstI C_TGCA'G
Cuts at: 830 2920 4554 4876 5338 5352 5414 5705 830
Size: 2090 1634 322 462 14 62 291 3478
Fragments arranged by size:
3478 2090 1634 462 322 291 62 14
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8353
PvuII CAG'CTG
Cuts at: 2584 2584
Size: 8353
SalI G'TCGA_C
Cuts at: 1936 5695 1936
Size: 3759 4594
SapI GCTCTTCn'nnn_
Cuts at: 5211 5211
Size: 8353
SbfI CC_TGCA'GG
Cuts at: 5705 5705
Size: 8353
ScaI AGT'ACT
Cuts at: 3458 6601 8313 3458
Size: 3143 1712 3498
Fragments arranged by size:
3498 3143 1712
SexAI A'CCwGG_T
Cuts at: 3047 3519 3047
Size: 472 7881
SfcI C'TryA_G
Cuts at: 826 1045 2085 2916 4550 4872 5175 5236 5334
Size: 219 1040 831 1634 322 303 61 98
Cuts at: 5334 5348 5410 5701 6836 7517 7708 826
Size: 14 62 291 1135 681 191 1471
Fragments arranged by size:
1634 1471 1135 1040 831 681 322 303
291 219 191 98 62 61 14
SfoI GGC'GCC
Cuts at: 2035 2035
Size: 8353
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8353
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8353
SmaI CCC'GGG
Cuts at: 1872 5680 1872
Size: 3808 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3475 6457 7358 7620 820
Size: 197 926 1532 2982 901 262 1553
Fragments arranged by size:
2982 1553 1532 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6144 493
Size: 5651 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8353
SphI G_CATG'C
Cuts at: 1132 5711 1132
Size: 4579 3774
SspI AAT'ATT
Cuts at: 5 52 2686 3125 3929 6277 5
Size: 47 2634 439 804 2348 2081
Fragments arranged by size:
2634 2348 2081 804 439 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8353
StyI C'CwwG_G
Cuts at: 513 1065 2004 2958 3831 4712 5792 513
Size: 552 939 954 873 881 1080 3074
Fragments arranged by size:
3074 1080 954 939 881 873 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3073 3082 3317 4195 4222 5048 6380
Size: 1255 1493 9 235 878 27 826 1332
Cuts at: 6380 6397 6550 6735 7180 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1493 1332 1255 878 826 445 235
185 153 27 17 9
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2625 2978 3456
Size: 274 80 33 51 1443 648 353 478
Cuts at: 3456 4121 5356 5556 6599 8311 96
Size: 665 1235 200 1043 1712 138
Fragments arranged by size:
1712 1443 1235 1043 665 648 478 353
274 200 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2224 2256 2301 2333 8313 8345 2224
Size: 32 45 32 5980 32 2232
Fragments arranged by size:
5980 2232 45 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8353
XbaI T'CTAG_A
Cuts at: 3992 5689 3992
Size: 1697 6656
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3400 3400
Size: 8353
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8353
XmaI C'CCGG_G
Cuts at: 1870 5678 1870
Size: 3808 4545
XmnI GAAnn'nnTTC
Cuts at: 6482 6482
Size: 8353
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2149 6161 276
Size: 53 83 186 936 615 4012 2468
Fragments arranged by size:
4012 2468 936 615 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbvCI BplI BsiWI
BstZ17I EcoICRI FalI FseI FspAI NaeI NgoMIV NruI
PacI PspOMI PspXI RsrII SacI SacII SanDI SfiI
SrfI SwaI