Restriction map for
pFN21AE5824
(Circular) (Six-base) MAPSORT of: pFN21AE5824.seq Check: 2992 from: 1 to: 7981
pFN21AE5824 7981 bp
With 182 enzymes: SgfI *
December 8, 2014 13:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5791 278
Size: 53 83 186 936 4255 2468
Fragments arranged by size:
4255 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5324 1937
Size: 3387 4594
Acc65I G'GTAC_C
Cuts at: 5302 5302
Size: 7981
AclI AA'CG_TT
Cuts at: 1563 6108 6481 1563
Size: 4545 373 3063
Fragments arranged by size:
4545 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2945 3456 3834 3891 4353 4530 4608 6044
Size: 1313 511 378 57 462 177 78 1436
Cuts at: 6044 7428 7696 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1436 1384 1313 511 462 378 268
177 78 57
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7784
AflIII A'CryG_T
Cuts at: 1203 4153 6880 7857 1203
Size: 2950 2727 977 1327
Fragments arranged by size:
2950 2727 1327 977
AgeI A'CCGG_T
Cuts at: 6812 6812
Size: 7981
AhdI GACnn_n'nnGTC
Cuts at: 6710 6710
Size: 7981
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7981
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3321 1184
Size: 2137 5844
AlwNI CAG_nnn'CTG
Cuts at: 2057 4243 5724 7296 2057
Size: 2186 1481 1572 2742
Fragments arranged by size:
2742 2186 1572 1481
ApaLI G'TGCA_C
Cuts at: 2741 6038 7194 2741
Size: 3297 1156 3528
Fragments arranged by size:
3528 3297 1156
ApoI r'AATT_y
Cuts at: 1493 2104 2365 2946 3455 3808 3858 4914 5033
Size: 611 261 581 509 353 50 1056 119
Cuts at: 5033 5290 5516 5668 7777 1493
Size: 257 226 152 2109 1697
Fragments arranged by size:
2109 1697 1056 611 581 509 353 261
257 226 152 119 50
AseI AT'TA_AT
Cuts at: 160 1032 6535 160
Size: 872 5503 1606
Fragments arranged by size:
5503 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7981
AvaI C'yCGr_G
Cuts at: 1870 1943 5306 1870
Size: 73 3363 4545
Fragments arranged by size:
4545 3363 73
BamHI G'GATC_C
Cuts at: 5311 5678 5311
Size: 367 7614
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3162 4771 5302 6757 618
Size: 325 217 627 1375 1609 531 1455 1842
Fragments arranged by size:
1842 1609 1455 1375 627 531 325 217
BanII G_rGCy'C
Cuts at: 1391 2252 4588 5300 1391
Size: 861 2336 712 4072
Fragments arranged by size:
4072 2336 861 712
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4644 4719 5183 928
Size: 971 2745 75 464 3726
Fragments arranged by size:
3726 2745 971 464 75
BbvCI CC'TCA_GC
Cuts at: 3625 5209 3625
Size: 1584 6397
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6172 6206 1903
Size: 34 4235 34 3678
Fragments arranged by size:
4235 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2735 4447 5873 7083 2735
Size: 1712 1426 1210 3633
Fragments arranged by size:
3633 1712 1426 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7981
BfrBI ATG'CAT
Cuts at: 3308 3308
Size: 7981
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6592 136
Size: 107 122 71 631 605 4920 1525
Fragments arranged by size:
4920 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7976 7976
Size: 7981
BlpI GC'TnA_GC
Cuts at: 5398 5398
Size: 7981
Bme1580I G_kGCm'C
Cuts at: 1790 2745 4776 6042 7198 1790
Size: 955 2031 1266 1156 2573
Fragments arranged by size:
2573 2031 1266 1156 955
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7981
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5712 6670 442
Size: 416 53 83 399 24 4295 958 1753
Fragments arranged by size:
4295 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7981
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4607 4607
Size: 7981
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2666 6641 1239
Size: 1427 3975 2579
Fragments arranged by size:
3975 2579 1427
Bpu10I CC'TnA_GC
Cuts at: 3625 5209 3625
Size: 1584 6397
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6106 6971 7269 6106
Size: 865 298 6818
Fragments arranged by size:
6818 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2912 4791 6644 882
Size: 617 1413 1879 1853 2219
Fragments arranged by size:
2219 1879 1853 1413 617
BsaAI yAC'GTr
Cuts at: 493 1204 4435 5772 493
Size: 711 3231 1337 2702
Fragments arranged by size:
3231 2702 1337 711
BsaBI GATnn'nnATC
Cuts at: 2623 5262 7975 2623
Size: 2639 2713 2629
Fragments arranged by size:
2713 2639 2629
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5788 6170 6805 275
Size: 53 83 186 936 4255 382 635 1451
Fragments arranged by size:
4255 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6414 6812 7086 7233 7644 6414
Size: 398 274 147 411 6751
Fragments arranged by size:
6751 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7756
Fragments arranged by size:
7756 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4461 5197 1186
Size: 3275 736 3970
Fragments arranged by size:
3970 3275 736
BseYI C'CCAG_C
Cuts at: 2967 7184 7680 2967
Size: 4217 496 3268
Fragments arranged by size:
4217 3268 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7408
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5431 6192 7220 1540
Size: 275 186 3430 761 1028 2301
Fragments arranged by size:
3430 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2745 2783 4588 6042 6127 7198 2745
Size: 38 1805 1454 85 1071 3528
Fragments arranged by size:
3528 1805 1454 1071 85 38
BsmI GAATG_Cn'
Cuts at: 5492 5585 5492
Size: 93 7888
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2252 2745 2783 4588 4776 5300 6042
Size: 399 462 493 38 1805 188 524 742
Cuts at: 6042 6127 7198 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1805 1071 742 524 493 462 399
188 85 38
BspEI T'CCGG_A
Cuts at: 7644 7644
Size: 7981
BspHI T'CATG_A
Cuts at: 4906 5870 7600 4906
Size: 964 1730 5287
Fragments arranged by size:
5287 1730 964
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5336 844
Size: 4492 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2515 5868 1394
Size: 429 692 3353 3507
Fragments arranged by size:
3507 3353 692 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6476 6650 65
Size: 1183 5228 174 1396
Fragments arranged by size:
5228 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6625 6812 1700
Size: 110 4815 187 2869
Fragments arranged by size:
4815 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2621 2657 4890 96
Size: 2525 36 2233 3187
Fragments arranged by size:
3187 2525 2233 36
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7981
BssSI C'ACGA_G
Cuts at: 6041 7053 6041
Size: 1012 6969
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5219 794
Size: 4425 3556
BstBI TT'CG_AA
Cuts at: 4750 6785 4750
Size: 2035 5946
BstEII G'GTnAC_C
Cuts at: 1184 3133 1184
Size: 1949 6032
BstYI r'GATC_y
Cuts at: 1972 2224 2563 5311 5678 6063 6080 7532 7618
Size: 252 339 2748 367 385 17 1452 86
Cuts at: 7618 7630 7976 1972
Size: 12 346 1977
Fragments arranged by size:
2748 1977 1452 385 367 346 339 252
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 7429
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7242
Fragments arranged by size:
7242 683 56
BtsI GCAGTG_nn'
Cuts at: 2237 3098 5497 6291 6311 2237
Size: 861 2399 794 20 3907
Fragments arranged by size:
3907 2399 861 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7981
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7946
DraI TTT'AAA
Cuts at: 4402 5285 4402
Size: 883 7098
DraIII CAC_nnn'GTG
Cuts at: 2086 4325 2086
Size: 2239 5742
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6988 809
Size: 507 5672 1802
Fragments arranged by size:
5672 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5428 6317 7946
Size: 54 1425 50 275 18 3598 889 1629
Cuts at: 7946 8
Size: 43
Fragments arranged by size:
3598 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5428 1537
Size: 275 3616 4090
Fragments arranged by size:
4090 3616 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2318 2745 3059 3119 5911 1353
Size: 965 427 314 60 2792 3423
Fragments arranged by size:
3423 2792 965 427 314 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6568 6942 7088 387
Size: 1064 138 4979 374 146 1280
Fragments arranged by size:
4979 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4586 4586
Size: 7981
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2666 2945 3456 3834 3891 4353 4530
Size: 393 1034 279 511 378 57 462 177
Cuts at: 4530 4608 6044 6641 7428 7696 1239
Size: 78 1436 597 787 268 1524
Fragments arranged by size:
1524 1436 1034 787 597 511 462 393
378 279 268 177 78 57
EcoO109I rG'GnC_Cy
Cuts at: 4496 4496
Size: 7981
EcoRI G'AATT_C
Cuts at: 3858 5033 5290 5668 3858
Size: 1175 257 378 6171
Fragments arranged by size:
6171 1175 378 257
EcoRV GAT'ATC
Cuts at: 2070 2070
Size: 7981
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3344 3344
Size: 7981
FspI TGC'GCA
Cuts at: 6487 6487
Size: 7981
HaeII r_GCGC'y
Cuts at: 1678 7128 1678
Size: 5450 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 1999 2031 2435 2467 3608
Size: 32 135 32 427 32 404 32 1141
Cuts at: 3608 3640 4787 4819 6622 6654 6696 6728 1373
Size: 32 1147 32 1803 32 42 32 2626
Fragments arranged by size:
2626 1803 1147 1141 427 404 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2480 2545 3745 5325 5571 669
Size: 988 281 542 65 1200 1580 246 3079
Fragments arranged by size:
3079 1580 1200 988 542 281 246 65
HindIII A'AGCT_T
Cuts at: 748 3816 3932 748
Size: 3068 116 4797
Fragments arranged by size:
4797 3068 116
HpaI GTT'AAC
Cuts at: 5571 5571
Size: 7981
KpnI G_GTAC'C
Cuts at: 5306 5306
Size: 7981
MfeI C'AATT_G
Cuts at: 2855 3199 4839 5580 2855
Size: 344 1640 741 5256
Fragments arranged by size:
5256 1640 741 344
MluI A'CGCG_T
Cuts at: 7857 7857
Size: 7981
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 7095 7279 1305
Size: 5790 184 2007
Fragments arranged by size:
5790 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6159
Fragments arranged by size:
6159 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3257 4530 4536 5256 5939 6298 6457
Size: 648 2091 1273 6 720 683 359 159
Cuts at: 6457 518
Size: 2042
Fragments arranged by size:
2091 2042 1273 720 683 648 359 159
6
MspA1I CmG'CkG
Cuts at: 1606 2501 3629 3698 4006 4520 5397 6074 7222
Size: 895 1128 69 308 514 877 677 1148
Cuts at: 7222 7467 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 1128 895 877 677 514 308
245 69
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7429
NdeI CA'TA_TG
Cuts at: 387 3120 387
Size: 2733 5248
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7981
NotI GC'GGCC_GC
Cuts at: 5428 5428
Size: 7981
NsiI A_TGCA'T
Cuts at: 3310 3310
Size: 7981
NspI r_CATG'y
Cuts at: 1132 1229 3994 4535 4866 5339 6884 1132
Size: 97 2765 541 331 473 1545 2229
Fragments arranged by size:
2765 2229 1545 541 473 331 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7981
PciI A'CATG_T
Cuts at: 6880 6880
Size: 7981
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2045 2086 3952 1108
Size: 937 41 1866 5137
Fragments arranged by size:
5137 1866 937 41
PmeI GTTT'AAAC
Cuts at: 5285 5285
Size: 7981
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6050 6082 7563 7595 6050
Size: 32 1481 32 6436
Fragments arranged by size:
6436 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7981
PsiI TTA'TAA
Cuts at: 3377 5551 3377
Size: 2174 5807
PstI C_TGCA'G
Cuts at: 830 2232 5333 830
Size: 1402 3101 3478
Fragments arranged by size:
3478 3101 1402
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7981
PvuII CAG'CTG
Cuts at: 2501 3629 3698 4006 4520 2501
Size: 1128 69 308 514 5962
Fragments arranged by size:
5962 1128 514 308 69
SacI G_AGCT'C
Cuts at: 4588 4588
Size: 7981
SalI G'TCGA_C
Cuts at: 1936 5323 1936
Size: 3387 4594
SapI GCTCTTCn'nnn_
Cuts at: 2318 3059 2318
Size: 741 7240
SbfI CC_TGCA'GG
Cuts at: 5333 5333
Size: 7981
ScaI AGT'ACT
Cuts at: 6229 7941 6229
Size: 1712 6269
SfcI C'TryA_G
Cuts at: 826 1045 2228 2571 3840 4194 5329 6464 7145
Size: 219 1183 343 1269 354 1135 1135 681
Cuts at: 7145 7336 826
Size: 191 1471
Fragments arranged by size:
1471 1269 1183 1135 1135 681 354 343
219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7981
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7981
SmaI CCC'GGG
Cuts at: 1872 5308 1872
Size: 3436 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 6085 6986 7248 820
Size: 197 926 4142 901 262 1553
Fragments arranged by size:
4142 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5772 493
Size: 5279 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7981
SphI G_CATG'C
Cuts at: 1132 4866 5339 1132
Size: 3734 473 3774
Fragments arranged by size:
3774 3734 473
SspI AAT'ATT
Cuts at: 5 52 2904 3751 5905 5
Size: 47 2852 847 2154 2081
Fragments arranged by size:
2852 2154 2081 847 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7981
StyI C'CwwG_G
Cuts at: 513 1065 2508 5420 513
Size: 552 1443 2912 3074
Fragments arranged by size:
3074 2912 1443 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3180 3602 4542 6008 6025 6178 6363
Size: 1255 1600 422 940 1466 17 153 185
Cuts at: 6363 6808 325
Size: 445 1498
Fragments arranged by size:
1600 1498 1466 1255 940 445 422 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2621 2657 4000
Size: 274 80 33 51 1443 644 36 1343
Cuts at: 4000 4890 6227 7939 96
Size: 890 1337 1712 138
Fragments arranged by size:
1712 1443 1343 1337 890 644 274 138
80 51 36 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2216 2248 4858 4890 7941 7973 2216
Size: 32 2610 32 3051 32 2224
Fragments arranged by size:
3051 2610 2224 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7981
XbaI T'CTAG_A
Cuts at: 4762 5317 4762
Size: 555 7426
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4604 4604
Size: 7981
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7981
XmaI C'CCGG_G
Cuts at: 1870 5306 1870
Size: 3436 4545
XmnI GAAnn'nnTTC
Cuts at: 6110 6110
Size: 7981
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5789 276
Size: 53 83 186 936 4255 2468
Fragments arranged by size:
4255 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI