Restriction map for
pFN21AE5818
(Circular) (Six-base) MAPSORT of: pFN21AE5818.seq Check: 5814 from: 1 to: 7570
pFN21AE5818 7570 bp
With 182 enzymes: SgfI *
December 8, 2014 13:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5380 278
Size: 53 83 186 936 3844 2468
Fragments arranged by size:
3844 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4913 1937
Size: 2976 4594
Acc65I G'GTAC_C
Cuts at: 4891 4891
Size: 7570
AclI AA'CG_TT
Cuts at: 1563 2762 5697 6070 1563
Size: 1199 2935 373 3063
Fragments arranged by size:
3063 2935 1199 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2250 4091 4142 5633 7017 7285 1632
Size: 618 1841 51 1491 1384 268 1917
Fragments arranged by size:
1917 1841 1491 1384 618 268 51
AflII C'TTAA_G
Cuts at: 820 1017 4562 820
Size: 197 3545 3828
Fragments arranged by size:
3828 3545 197
AflIII A'CryG_T
Cuts at: 1203 6469 7446 1203
Size: 5266 977 1327
Fragments arranged by size:
5266 1327 977
AgeI A'CCGG_T
Cuts at: 6401 6401
Size: 7570
AhdI GACnn_n'nnGTC
Cuts at: 6299 6299
Size: 7570
AleI CACnn'nnGTG
Cuts at: 1166 3870 3921 1166
Size: 2704 51 4815
Fragments arranged by size:
4815 2704 51
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7570
AlwNI CAG_nnn'CTG
Cuts at: 3662 3761 5313 6885 3662
Size: 99 1552 1572 4347
Fragments arranged by size:
4347 1572 1552 99
ApaLI G'TGCA_C
Cuts at: 5627 6783 5627
Size: 1156 6414
ApoI r'AATT_y
Cuts at: 1493 3736 4236 4256 4308 4320 4338 4497 4879
Size: 2243 500 20 52 12 18 159 382
Cuts at: 4879 5105 5257 7366 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2243 2109 1697 500 382 226 159 152
52 20 18 12
AseI AT'TA_AT
Cuts at: 160 1032 6124 160
Size: 872 5092 1606
Fragments arranged by size:
5092 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7570
AvaI C'yCGr_G
Cuts at: 1870 1943 4549 4895 1870
Size: 73 2606 346 4545
Fragments arranged by size:
4545 2606 346 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3667 3700 3667
Size: 33 7537
BamHI G'GATC_C
Cuts at: 2730 4900 5267 2730
Size: 2170 367 5033
Fragments arranged by size:
5033 2170 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3872 4609 4891 6346 618
Size: 325 217 627 2085 737 282 1455 1842
Fragments arranged by size:
2085 1842 1455 737 627 325 282 217
BanII G_rGCy'C
Cuts at: 1391 4889 1391
Size: 3498 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2041 3280 928
Size: 971 142 1239 5218
Fragments arranged by size:
5218 1239 971 142
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3493 3527 5761 5795 1903
Size: 34 1556 34 2234 34 3678
Fragments arranged by size:
3678 2234 1556 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5462 6672 5462
Size: 1210 6360
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7570
BfrBI ATG'CAT
Cuts at: 4361 4361
Size: 7570
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2659 6181
Size: 107 122 71 631 605 337 650 3522
Cuts at: 6181 136
Size: 1525
Fragments arranged by size:
3522 1525 650 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 7565 7565
Size: 7570
BlpI GC'TnA_GC
Cuts at: 4987 4987
Size: 7570
Bme1580I G_kGCm'C
Cuts at: 1790 4614 5631 6787 1790
Size: 2824 1017 1156 2573
Fragments arranged by size:
2824 2573 1156 1017
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7570
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3379 3394 3409
Size: 416 53 83 399 24 1962 15 15
Cuts at: 3409 5301 6259 442
Size: 1892 958 1753
Fragments arranged by size:
1962 1892 1753 958 416 399 83 53
24 15 15
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7570
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2032 2032
Size: 7570
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2708 3350 4796 6230 1239
Size: 1469 642 1446 1434 2579
Fragments arranged by size:
2579 1469 1446 1434 642
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3901 5695 6560 6858 3901
Size: 1794 865 298 4613
Fragments arranged by size:
4613 1794 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4270 6233 882
Size: 617 2771 1963 2219
Fragments arranged by size:
2771 2219 1963 617
BsaAI yAC'GTr
Cuts at: 493 1204 5361 493
Size: 711 4157 2702
Fragments arranged by size:
4157 2702 711
BsaBI GATnn'nnATC
Cuts at: 7564 7564
Size: 7570
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3129 5377 5759 6394
Size: 53 83 186 936 1596 2248 382 635
Cuts at: 6394 275
Size: 1451
Fragments arranged by size:
2248 1596 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6003 6401 6675 6822 7233 6003
Size: 398 274 147 411 6340
Fragments arranged by size:
6340 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2289 2319 2444 2474 3270
Size: 30 165 30 886 30 125 30 796
Cuts at: 3270 3300 4772 4802 1178
Size: 30 1472 30 3946
Fragments arranged by size:
3946 1472 886 796 165 125 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2705 3410 4288 4548 1186
Size: 1519 705 878 260 4208
Fragments arranged by size:
4208 1519 878 705 260
BseYI C'CCAG_C
Cuts at: 2973 3091 4445 6773 7269 2973
Size: 118 1354 2328 496 3274
Fragments arranged by size:
3274 2328 1354 496 118
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4449 1163
Size: 573 2713 4284
Fragments arranged by size:
4284 2713 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5020 5781 6809 1540
Size: 275 186 3019 761 1028 2301
Fragments arranged by size:
3019 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3708 4683 5631 5716 6787 3708
Size: 975 948 85 1071 4491
Fragments arranged by size:
4491 1071 975 948 85
BsmI GAATG_Cn'
Cuts at: 2556 3839 4123 4480 5081 5174 2556
Size: 1283 284 357 601 93 4952
Fragments arranged by size:
4952 1283 601 357 284 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4814 4814
Size: 7570
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3708 4614 4683 4889 5631 5716 6787
Size: 399 1918 906 69 206 742 85 1071
Cuts at: 6787 1391
Size: 2174
Fragments arranged by size:
2174 1918 1071 906 742 399 206 85
69
BspEI T'CCGG_A
Cuts at: 7233 7233
Size: 7570
BspHI T'CATG_A
Cuts at: 2598 3222 5459 7189 2598
Size: 624 2237 1730 2979
Fragments arranged by size:
2979 2237 1730 624
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2586 2616 4925 844
Size: 1742 30 2309 3489
Fragments arranged by size:
3489 2309 1742 30
BsrBI CCG'CTC
Cuts at: 1394 1823 3445 5457 1394
Size: 429 1622 2012 3507
Fragments arranged by size:
3507 2012 1622 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6065 6239 65
Size: 1183 4817 174 1396
Fragments arranged by size:
4817 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3077 4867 6214 6401 1700
Size: 110 1267 1790 1347 187 2869
Fragments arranged by size:
2869 1790 1347 1267 187 110
BsrGI T'GTAC_A
Cuts at: 96 2376 96
Size: 2280 5290
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7570
BssSI C'ACGA_G
Cuts at: 4685 5630 6642 4685
Size: 945 1012 5613
Fragments arranged by size:
5613 1012 945
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2217 3359 794
Size: 1423 1142 5005
Fragments arranged by size:
5005 1423 1142
BstBI TT'CG_AA
Cuts at: 2929 4495 6374 2929
Size: 1566 1879 4125
Fragments arranged by size:
4125 1879 1566
BstEII G'GTnAC_C
Cuts at: 1184 2640 3256 1184
Size: 1456 616 5498
Fragments arranged by size:
5498 1456 616
BstXI CCAn_nnnn'nTGG
Cuts at: 2284 3923 2284
Size: 1639 5931
BstYI r'GATC_y
Cuts at: 1972 2730 3013 4900 5267 5652 5669 7121 7207
Size: 758 283 1887 367 385 17 1452 86
Cuts at: 7207 7219 7565 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1887 1452 758 385 367 346 283
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4742 4742
Size: 7570
BtgI C'CryG_G
Cuts at: 513 1065 2004 2507 2935 513
Size: 552 939 503 428 5148
Fragments arranged by size:
5148 939 552 503 428
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3820 487
Size: 683 56 2594 4237
Fragments arranged by size:
4237 2594 683 56
BtsI GCAGTG_nn'
Cuts at: 2675 2706 4546 4625 5086 5880 5900 2675
Size: 31 1840 79 461 794 20 4345
Fragments arranged by size:
4345 1840 794 461 79 31 20
ClaI AT'CG_AT
Cuts at: 1554 2610 1554
Size: 1056 6514
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7535
DraI TTT'AAA
Cuts at: 4874 4874
Size: 7570
DraIII CAC_nnn'GTG
Cuts at: 4269 4687 4269
Size: 418 7152
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6577 809
Size: 507 5261 1802
Fragments arranged by size:
5261 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2166 4865 5017
Size: 54 1425 50 275 18 336 2699 152
Cuts at: 5017 5906 7535 8
Size: 889 1629 43
Fragments arranged by size:
2699 1629 1425 889 336 275 152 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5017 1537
Size: 275 3205 4090
Fragments arranged by size:
4090 3205 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2023 2241 3092 3387 3903 4136 4753 5500
Size: 670 218 851 295 516 233 617 747
Cuts at: 5500 1353
Size: 3423
Fragments arranged by size:
3423 851 747 670 617 516 295 233
218
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6157 6531 6677 387
Size: 1064 138 4568 374 146 1280
Fragments arranged by size:
4568 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2250 2708 3350 4091 4142 4796 5633
Size: 393 618 458 642 741 51 654 837
Cuts at: 5633 6230 7017 7285 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 837 787 741 654 642 618 597
458 393 268 51
EcoNI CCTnn'n_nnAGG
Cuts at: 2897 3880 4428 2897
Size: 983 548 6039
Fragments arranged by size:
6039 983 548
EcoRI G'AATT_C
Cuts at: 4320 4338 4879 5257 4320
Size: 18 541 378 6633
Fragments arranged by size:
6633 541 378 18
EcoRV GAT'ATC
Cuts at: 3184 3184
Size: 7570
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2571 2571
Size: 7570
FspI TGC'GCA
Cuts at: 6076 6076
Size: 7570
HaeII r_GCGC'y
Cuts at: 1678 6717 1678
Size: 5039 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2444 2476 2946 2978 3099
Size: 32 135 32 872 32 470 32 121
Cuts at: 3099 3131 3270 3302 4502 4514 4534 4546 6211
Size: 32 139 32 1200 12 20 12 1665
Cuts at: 6211 6243 6285 6317 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1665 1200 872 470 139 135 121
42 32 32 32 32 32 32 32
32 20 12 12
HincII GTy'rAC
Cuts at: 669 1657 1938 4914 5160 669
Size: 988 281 2976 246 3079
Fragments arranged by size:
3079 2976 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7570
HpaI GTT'AAC
Cuts at: 5160 5160
Size: 7570
KpnI G_GTAC'C
Cuts at: 4895 4895
Size: 7570
MfeI C'AATT_G
Cuts at: 5169 5169
Size: 7570
MluI A'CGCG_T
Cuts at: 7446 7446
Size: 7570
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2412 6684 6868 1305
Size: 1107 4272 184 2007
Fragments arranged by size:
4272 2007 1107 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2168 10
Size: 54 1425 343 336 5412
Fragments arranged by size:
5412 1425 343 336 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2372 3080 3870 3921 5528 5887 6046
Size: 648 1206 708 790 51 1607 359 159
Cuts at: 6046 518
Size: 2042
Fragments arranged by size:
2042 1607 1206 790 708 648 359 159
51
MspA1I CmG'CkG
Cuts at: 1606 2089 3095 3240 4986 5663 6811 7056 1606
Size: 483 1006 145 1746 677 1148 245 2120
Fragments arranged by size:
2120 1746 1148 1006 677 483 245 145
NcoI C'CATG_G
Cuts at: 513 1065 2004 2507 2935 513
Size: 552 939 503 428 5148
Fragments arranged by size:
5148 939 552 503 428
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7570
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7570
NotI GC'GGCC_GC
Cuts at: 5017 5017
Size: 7570
NsiI A_TGCA'T
Cuts at: 4363 4363
Size: 7570
NspI r_CATG'y
Cuts at: 1132 1229 2504 3085 4643 4928 6473 1132
Size: 97 1275 581 1558 285 1545 2229
Fragments arranged by size:
2229 1558 1545 1275 581 285 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7570
PciI A'CATG_T
Cuts at: 6469 6469
Size: 7570
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3010 3461 1108
Size: 1902 451 5217
Fragments arranged by size:
5217 1902 451
PfoI T'CCnGG_A
Cuts at: 3327 3327
Size: 7570
PmeI GTTT'AAAC
Cuts at: 4874 4874
Size: 7570
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5639 5671 7152 7184 5639
Size: 32 1481 32 6025
Fragments arranged by size:
6025 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7570
PsiI TTA'TAA
Cuts at: 3962 4413 5140 3962
Size: 451 727 6392
Fragments arranged by size:
6392 727 451
PstI C_TGCA'G
Cuts at: 830 4574 4632 4922 830
Size: 3744 58 290 3478
Fragments arranged by size:
3744 3478 290 58
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7570
PvuII CAG'CTG
Cuts at: 2089 3095 3240 2089
Size: 1006 145 6419
Fragments arranged by size:
6419 1006 145
SalI G'TCGA_C
Cuts at: 1936 4912 1936
Size: 2976 4594
SapI GCTCTTCn'nnn_
Cuts at: 2023 3092 2023
Size: 1069 6501
SbfI CC_TGCA'GG
Cuts at: 4922 4922
Size: 7570
ScaI AGT'ACT
Cuts at: 2263 4568 4769 5818 7530 2263
Size: 2305 201 1049 1712 2303
Fragments arranged by size:
2305 2303 1712 1049 201
SfcI C'TryA_G
Cuts at: 826 1045 3177 4570 4628 4918 6053 6734 6925
Size: 219 2132 1393 58 290 1135 681 191
Cuts at: 6925 826
Size: 1471
Fragments arranged by size:
2132 1471 1393 1135 681 290 219 191
58
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7570
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7570
SmaI CCC'GGG
Cuts at: 1872 4897 1872
Size: 3025 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3880 4562 5674 6575 6837 820
Size: 197 926 1937 682 1112 901 262 1553
Fragments arranged by size:
1937 1553 1112 926 901 682 262 197
SnaBI TAC'GTA
Cuts at: 493 5361 493
Size: 4868 2702
SpeI A'CTAG_T
Cuts at: 152 2258 152
Size: 2106 5464
SphI G_CATG'C
Cuts at: 1132 2504 3085 4928 1132
Size: 1372 581 1843 3774
Fragments arranged by size:
3774 1843 1372 581
SspI AAT'ATT
Cuts at: 5 52 2182 3531 5494 5
Size: 47 2130 1349 1963 2081
Fragments arranged by size:
2130 2081 1963 1349 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7570
StyI C'CwwG_G
Cuts at: 513 1065 2004 2169 2507 2518 2935 3252 4435
Size: 552 939 165 338 11 417 317 1183
Cuts at: 4435 5009 513
Size: 574 3074
Fragments arranged by size:
3074 1183 939 574 552 417 338 317
165 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2070 3882 5597 5614 5767 5952 6397
Size: 1255 490 1812 1715 17 153 185 445
Cuts at: 6397 325
Size: 1498
Fragments arranged by size:
1812 1715 1498 1255 490 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2261 2376 4566
Size: 274 80 33 51 1443 284 115 2190
Cuts at: 4566 4767 5816 7528 96
Size: 201 1049 1712 138
Fragments arranged by size:
2190 1712 1443 1049 284 274 201 138
115 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2289 2321 7530 7562 2289
Size: 32 5209 32 2297
Fragments arranged by size:
5209 2297 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7570
XbaI T'CTAG_A
Cuts at: 4906 4906
Size: 7570
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4442 4442
Size: 7570
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7570
XmaI C'CCGG_G
Cuts at: 1870 4895 1870
Size: 3025 4545
XmnI GAAnn'nnTTC
Cuts at: 3776 3989 4152 5699 3776
Size: 213 163 1547 5647
Fragments arranged by size:
5647 1547 213 163
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5378 276
Size: 53 83 186 936 3844 2468
Fragments arranged by size:
3844 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
Bpu10I BsiWI BstZ17I EcoICRI EcoO109I FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI