Restriction map for
pFN21AE5816
(Circular) (Six-base) MAPSORT of: pFN21AE5816.seq Check: 6001 from: 1 to: 7291
pFN21AE5816 7291 bp
With 182 enzymes: SgfI *
December 8, 2014 13:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5101 278
Size: 53 83 186 936 3565 2468
Fragments arranged by size:
3565 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4634 1937
Size: 2697 4594
Acc65I G'GTAC_C
Cuts at: 4612 4612
Size: 7291
AclI AA'CG_TT
Cuts at: 1563 5418 5791 1563
Size: 3855 373 3063
Fragments arranged by size:
3855 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2318 2623 3274 3506 3588 3918 4451 5354
Size: 686 305 651 232 82 330 533 903
Cuts at: 5354 6738 7006 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 903 686 651 533 330 305
268 232 82
AfeI AGC'GCT
Cuts at: 2141 2141
Size: 7291
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7094
AflIII A'CryG_T
Cuts at: 1203 2097 6190 7167 1203
Size: 894 4093 977 1327
Fragments arranged by size:
4093 1327 977 894
AgeI A'CCGG_T
Cuts at: 6122 6122
Size: 7291
AhdI GACnn_n'nnGTC
Cuts at: 6020 6020
Size: 7291
AleI CACnn'nnGTG
Cuts at: 1166 3983 1166
Size: 2817 4474
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7291
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4052 4084 4052
Size: 32 7259
AlwNI CAG_nnn'CTG
Cuts at: 2413 2440 5034 6606 2413
Size: 27 2594 1572 3098
Fragments arranged by size:
3098 2594 1572 27
ApaI G_GGCC'C
Cuts at: 3705 3705
Size: 7291
ApaLI G'TGCA_C
Cuts at: 2543 2611 5348 6504 2543
Size: 68 2737 1156 3330
Fragments arranged by size:
3330 2737 1156 68
ApoI r'AATT_y
Cuts at: 1493 2777 3056 3932 4252 4259 4294 4600 4826
Size: 1284 279 876 320 7 35 306 226
Cuts at: 4826 4978 7087 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1284 876 320 306 279 226
152 35 7
AseI AT'TA_AT
Cuts at: 160 1032 5845 160
Size: 872 4813 1606
Fragments arranged by size:
4813 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7291
AvaI C'yCGr_G
Cuts at: 1870 1943 2129 4074 4616 1870
Size: 73 186 1945 542 4545
Fragments arranged by size:
4545 1945 542 186 73
BamHI G'GATC_C
Cuts at: 2086 4621 4988 2086
Size: 2535 367 4389
Fragments arranged by size:
4389 2535 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2114 2564 3821 4612 6067
Size: 325 217 627 327 450 1257 791 1455
Cuts at: 6067 618
Size: 1842
Fragments arranged by size:
1842 1455 1257 791 627 450 327 325
217
BanII G_rGCy'C
Cuts at: 1391 2739 3155 3633 3705 4610 1391
Size: 1348 416 478 72 905 4072
Fragments arranged by size:
4072 1348 905 478 416 72
BbeI G_GCGC'C
Cuts at: 2568 2568
Size: 7291
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2718 4274 928
Size: 971 819 1556 3945
Fragments arranged by size:
3945 1556 971 819
BbvCI CC'TCA_GC
Cuts at: 2054 2054
Size: 7291
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2441 2475 5482 5516 1903
Size: 34 504 34 3007 34 3678
Fragments arranged by size:
3678 3007 504 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5183 6393 5183
Size: 1210 6081
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7291
BfrBI ATG'CAT
Cuts at: 3221 3221
Size: 7291
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3497 5902 136
Size: 107 122 71 631 605 1825 2405 1525
Fragments arranged by size:
2405 1825 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3711 7286 3711
Size: 3575 3716
BlpI GC'TnA_GC
Cuts at: 2420 2738 3653 4708 2420
Size: 318 915 1055 5003
Fragments arranged by size:
5003 1055 915 318
Bme1580I G_kGCm'C
Cuts at: 1790 2117 2547 2615 3705 5352 6508 1790
Size: 327 430 68 1090 1647 1156 2573
Fragments arranged by size:
2573 1647 1156 1090 430 327 68
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7291
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5022 5980 442
Size: 416 53 83 399 24 3605 958 1753
Fragments arranged by size:
3605 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7291
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3678 4100 3678
Size: 422 6869
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2213 2753 4438 5951 1239
Size: 974 540 1685 1513 2579
Fragments arranged by size:
2579 1685 1513 974 540
Bpu10I CC'TnA_GC
Cuts at: 2054 2054
Size: 7291
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2543 4473 4496 5416 6281 6579 2543
Size: 1930 23 920 865 298 3255
Fragments arranged by size:
3255 1930 920 865 298 23
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5954 882
Size: 617 4455 2219
Fragments arranged by size:
4455 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3403 3511 3523 5082 493
Size: 711 2199 108 12 1559 2702
Fragments arranged by size:
2702 2199 1559 711 108 12
BsaBI GATnn'nnATC
Cuts at: 7285 7285
Size: 7291
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2126 2565 5098 5480
Size: 53 83 186 936 593 439 2533 382
Cuts at: 5480 6115 275
Size: 635 1451
Fragments arranged by size:
2533 1451 936 635 593 439 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2181 5724 6122 6396 6543 6954 2181
Size: 3543 398 274 147 411 2518
Fragments arranged by size:
3543 2518 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7066
Fragments arranged by size:
7066 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2147 2150 2210 3163 3398 4058 1186
Size: 961 3 60 953 235 660 4419
Fragments arranged by size:
4419 961 953 660 235 60 3
BseYI C'CCAG_C
Cuts at: 3835 6494 6990 3835
Size: 2659 496 4136
Fragments arranged by size:
4136 2659 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3145 3392 3524 3545 1163
Size: 573 1409 247 132 21 4909
Fragments arranged by size:
4909 1409 573 247 132 21
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4741 5502 6530 1540
Size: 275 186 2740 761 1028 2301
Fragments arranged by size:
2740 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2274 2547 2615 2739 5352 5437 6508 2274
Size: 273 68 124 2613 85 1071 3057
Fragments arranged by size:
3057 2613 1071 273 124 85 68
BsmI GAATG_Cn'
Cuts at: 3223 3371 4802 4895 3223
Size: 148 1431 93 5619
Fragments arranged by size:
5619 1431 148 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2117 2274 2547 2615 2739 3155 3633
Size: 399 327 157 273 68 124 416 478
Cuts at: 3633 3705 4610 5352 5437 6508 1391
Size: 72 905 742 85 1071 2174
Fragments arranged by size:
2174 1071 905 742 478 416 399 327
273 157 124 85 72 68
BspEI T'CCGG_A
Cuts at: 2181 6954 2181
Size: 4773 2518
BspHI T'CATG_A
Cuts at: 3159 3783 4353 5180 6910 3159
Size: 624 570 827 1730 3540
Fragments arranged by size:
3540 1730 827 624 570
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3429 4129 4646 844
Size: 2585 700 517 3489
Fragments arranged by size:
3489 2585 700 517
BsrBI CCG'CTC
Cuts at: 1394 1823 2578 5178 1394
Size: 429 755 2600 3507
Fragments arranged by size:
3507 2600 755 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2611 2816 3666 5786 5960 65
Size: 1183 1363 205 850 2120 174 1396
Fragments arranged by size:
2120 1396 1363 1183 850 205 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2040 5935 6122 1700
Size: 110 230 3895 187 2869
Fragments arranged by size:
3895 2869 230 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7291
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7291
BssSI C'ACGA_G
Cuts at: 5351 6363 5351
Size: 1012 6279
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2551 2610 2998 794
Size: 1757 59 388 5087
Fragments arranged by size:
5087 1757 388 59
BstBI TT'CG_AA
Cuts at: 6095 6095
Size: 7291
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7291
BstXI CCAn_nnnn'nTGG
Cuts at: 3451 3451
Size: 7291
BstYI r'GATC_y
Cuts at: 1972 2086 3711 4621 4988 5373 5390 6842 6928
Size: 114 1625 910 367 385 17 1452 86
Cuts at: 6928 6940 7286 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1625 1452 910 385 367 346 114
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3146 4565 3146
Size: 1419 5872
BtgI C'CryG_G
Cuts at: 513 1065 2259 3392 3492 3981 513
Size: 552 1194 1133 100 489 3823
Fragments arranged by size:
3823 1194 1133 552 489 100
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6552
Fragments arranged by size:
6552 683 56
BtsI GCAGTG_nn'
Cuts at: 2521 4807 5601 5621 2521
Size: 2286 794 20 4191
Fragments arranged by size:
4191 2286 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7291
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7256
DraI TTT'AAA
Cuts at: 2877 3865 4595 2877
Size: 988 730 5573
Fragments arranged by size:
5573 988 730
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6298 809
Size: 507 4982 1802
Fragments arranged by size:
4982 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2256 2340 4738
Size: 54 1425 50 275 18 426 84 2398
Cuts at: 4738 5627 7256 8
Size: 889 1629 43
Fragments arranged by size:
2398 1629 1425 889 426 275 84 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4738 1537
Size: 275 2926 4090
Fragments arranged by size:
4090 2926 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2853 5221 1353
Size: 1500 2368 3423
Fragments arranged by size:
3423 2368 1500
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3546 5878 6252 6398 387
Size: 1064 138 1957 2332 374 146 1280
Fragments arranged by size:
2332 1957 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2737 2737
Size: 7291
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2213 2318 2623 2753 3274 3506 3588
Size: 393 581 105 305 130 521 232 82
Cuts at: 3588 3918 4438 4451 5354 5951 6738 7006 1239
Size: 330 520 13 903 597 787 268 1524
Fragments arranged by size:
1524 903 787 597 581 521 520 393
330 305 268 232 130 105 82 13
EcoNI CCTnn'n_nnAGG
Cuts at: 3583 3583
Size: 7291
EcoO109I rG'GnC_Cy
Cuts at: 3702 3702
Size: 7291
EcoRI G'AATT_C
Cuts at: 4252 4294 4600 4978 4252
Size: 42 306 378 6565
Fragments arranged by size:
6565 378 306 42
EcoRV GAT'ATC
Cuts at: 3739 4450 3739
Size: 711 6580
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3361 3361
Size: 7291
FspI TGC'GCA
Cuts at: 2363 5797 2363
Size: 3434 3857
FspAI rTGC'GCAy
Cuts at: 2363 2363
Size: 7291
HaeII r_GCGC'y
Cuts at: 1678 2041 2143 2413 2568 6438 1678
Size: 363 102 270 155 3870 2531
Fragments arranged by size:
3870 2531 363 270 155 102
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4469 4501 5932 5964 6006
Size: 32 135 32 2897 32 1431 32 42
Cuts at: 6006 6038 1373
Size: 32 2626
Fragments arranged by size:
2897 2626 1431 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3038 4635 4881 669
Size: 988 281 1100 1597 246 3079
Fragments arranged by size:
3079 1597 1100 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7291
HpaI GTT'AAC
Cuts at: 4881 4881
Size: 7291
KasI G'GCGC_C
Cuts at: 2564 2564
Size: 7291
KpnI G_GTAC'C
Cuts at: 4616 4616
Size: 7291
MfeI C'AATT_G
Cuts at: 2996 4890 2996
Size: 1894 5397
MluI A'CGCG_T
Cuts at: 7167 7167
Size: 7291
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2931 6405 6589 1305
Size: 1626 3474 184 2007
Fragments arranged by size:
3474 2007 1626 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2342 10
Size: 54 1425 343 510 4959
Fragments arranged by size:
4959 1425 510 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2174 3449 3983 5249 5608 5767 518
Size: 648 1008 1275 534 1266 359 159 2042
Fragments arranged by size:
2042 1275 1266 1008 648 534 359 159
MspA1I CmG'CkG
Cuts at: 1606 2254 2428 2626 3505 4707 5384 6532 6777
Size: 648 174 198 879 1202 677 1148 245
Cuts at: 6777 1606
Size: 2120
Fragments arranged by size:
2120 1202 1148 879 677 648 245 198
174
NaeI GCC'GGC
Cuts at: 2042 2042
Size: 7291
NarI GG'CG_CC
Cuts at: 2565 2565
Size: 7291
NcoI C'CATG_G
Cuts at: 513 1065 3981 513
Size: 552 2916 3823
Fragments arranged by size:
3823 2916 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7291
NgoMIV G'CCGG_C
Cuts at: 2040 2040
Size: 7291
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7291
NotI GC'GGCC_GC
Cuts at: 4738 4738
Size: 7291
NsiI A_TGCA'T
Cuts at: 3223 3223
Size: 7291
NspI r_CATG'y
Cuts at: 1132 1229 2035 2101 4006 4249 4649 6194 1132
Size: 97 806 66 1905 243 400 1545 2229
Fragments arranged by size:
2229 1905 1545 806 400 243 97 66
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7291
PciI A'CATG_T
Cuts at: 2097 6190 2097
Size: 4093 3198
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7291
PfoI T'CCnGG_A
Cuts at: 2082 2145 3314 2082
Size: 63 1169 6059
Fragments arranged by size:
6059 1169 63
PmeI GTTT'AAAC
Cuts at: 4595 4595
Size: 7291
PmlI CAC'GTG
Cuts at: 3523 3523
Size: 7291
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5360 5392 6873 6905 5360
Size: 32 1481 32 5746
Fragments arranged by size:
5746 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7291
PsiI TTA'TAA
Cuts at: 3972 4861 3972
Size: 889 6402
PspOMI G'GGCC_C
Cuts at: 3701 3701
Size: 7291
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3935 3967 3935
Size: 32 7259
PstI C_TGCA'G
Cuts at: 830 2253 3711 4643 830
Size: 1423 1458 932 3478
Fragments arranged by size:
3478 1458 1423 932
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7291
PvuII CAG'CTG
Cuts at: 2254 3505 2254
Size: 1251 6040
SacI G_AGCT'C
Cuts at: 2739 2739
Size: 7291
SalI G'TCGA_C
Cuts at: 1936 4633 1936
Size: 2697 4594
SbfI CC_TGCA'GG
Cuts at: 4643 4643
Size: 7291
ScaI AGT'ACT
Cuts at: 5539 7251 5539
Size: 1712 5579
SfcI C'TryA_G
Cuts at: 826 1045 2249 3707 4639 5774 6455 6646 826
Size: 219 1204 1458 932 1135 681 191 1471
Fragments arranged by size:
1471 1458 1204 1135 932 681 219 191
SfoI GGC'GCC
Cuts at: 2566 2566
Size: 7291
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7291
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7291
SmaI CCC'GGG
Cuts at: 1872 4618 1872
Size: 2746 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2558 4475 4488 5395 6296 6558
Size: 197 926 615 1917 13 907 901 262
Cuts at: 6558 820
Size: 1553
Fragments arranged by size:
1917 1553 926 907 901 615 262 197
13
SnaBI TAC'GTA
Cuts at: 493 5082 493
Size: 4589 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7291
SphI G_CATG'C
Cuts at: 1132 4249 4649 1132
Size: 3117 400 3774
Fragments arranged by size:
3774 3117 400
SspI AAT'ATT
Cuts at: 5 52 5215 5
Size: 47 5163 2081
Fragments arranged by size:
5163 2081 47
StuI AGG'CCT
Cuts at: 1517 3087 1517
Size: 1570 5721
StyI C'CwwG_G
Cuts at: 513 1065 3981 4730 513
Size: 552 2916 749 3074
Fragments arranged by size:
3074 2916 749 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3243 5318 5335 5488 5673 6118 325
Size: 1255 1663 2075 17 153 185 445 1498
Fragments arranged by size:
2075 1663 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3804 5537 7249
Size: 274 80 33 51 1443 1827 1733 1712
Cuts at: 7249 96
Size: 138
Fragments arranged by size:
1827 1733 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3581 3613 7251 7283 3581
Size: 32 3638 32 3589
Fragments arranged by size:
3638 3589 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2717 4412 1316
Size: 1401 1695 4195
Fragments arranged by size:
4195 1695 1401
XbaI T'CTAG_A
Cuts at: 4627 4627
Size: 7291
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7291
XmaI C'CCGG_G
Cuts at: 1870 4616 1870
Size: 2746 4545
XmnI GAAnn'nnTTC
Cuts at: 5420 5420
Size: 7291
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5099 276
Size: 53 83 186 936 3565 2468
Fragments arranged by size:
3565 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PsrI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BsiWI BsmBI BstZ17I DraIII
FseI NruI PacI PpuMI PspXI RsrII SacII SanDI
SapI SexAI SfiI SrfI SwaI XcmI