Restriction map for
pFN21AE5814
(Circular) (Six-base) MAPSORT of: pFN21AE5814.seq Check: 6566 from: 1 to: 7444
pFN21AE5814 7444 bp
With 182 enzymes: SgfI *
December 8, 2014 13:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3646 3656 3646
Size: 10 7434
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4296 5254 278
Size: 53 83 186 936 2760 958 2468
Fragments arranged by size:
2760 2468 958 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4787 1937
Size: 2850 4594
Acc65I G'GTAC_C
Cuts at: 4765 4765
Size: 7444
AclI AA'CG_TT
Cuts at: 1563 5571 5944 1563
Size: 4008 373 3063
Fragments arranged by size:
4008 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2414 3306 3846 5507 6891 7159 1632
Size: 782 892 540 1661 1384 268 1917
Fragments arranged by size:
1917 1661 1384 892 782 540 268
AflII C'TTAA_G
Cuts at: 820 1017 3578 820
Size: 197 2561 4686
Fragments arranged by size:
4686 2561 197
AflIII A'CryG_T
Cuts at: 1203 3246 6343 7320 1203
Size: 2043 3097 977 1327
Fragments arranged by size:
3097 2043 1327 977
AgeI A'CCGG_T
Cuts at: 4550 6275 4550
Size: 1725 5719
AhdI GACnn_n'nnGTC
Cuts at: 3820 4263 6173 3820
Size: 443 1910 5091
Fragments arranged by size:
5091 1910 443
AleI CACnn'nnGTG
Cuts at: 1166 2190 4530 1166
Size: 1024 2340 4080
Fragments arranged by size:
4080 2340 1024
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2635 4194 1184
Size: 1451 1559 4434
Fragments arranged by size:
4434 1559 1451
AlwNI CAG_nnn'CTG
Cuts at: 5187 6759 5187
Size: 1572 5872
ApaI G_GGCC'C
Cuts at: 4164 4164
Size: 7444
ApaLI G'TGCA_C
Cuts at: 2177 4179 5501 6657 2177
Size: 2002 1322 1156 2964
Fragments arranged by size:
2964 2002 1322 1156
ApoI r'AATT_y
Cuts at: 1493 2784 3563 4012 4116 4472 4753 4979 5131
Size: 1291 779 449 104 356 281 226 152
Cuts at: 5131 7240 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1291 779 449 356 281 226
152 104
AseI AT'TA_AT
Cuts at: 160 1032 2040 4092 5998 160
Size: 872 1008 2052 1906 1606
Fragments arranged by size:
2052 1906 1606 1008 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7444
AvaI C'yCGr_G
Cuts at: 1870 1943 4059 4359 4769 1870
Size: 73 2116 300 410 4545
Fragments arranged by size:
4545 2116 410 300 73
BamHI G'GATC_C
Cuts at: 3899 4735 4774 5141 3899
Size: 836 39 367 6202
Fragments arranged by size:
6202 836 367 39
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4765 6220 618
Size: 325 217 627 2978 1455 1842
Fragments arranged by size:
2978 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2990 3260 3470 4164 4515 4763 1391
Size: 1599 270 210 694 351 248 4072
Fragments arranged by size:
4072 1599 694 351 270 248 210
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6473
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5635 5669 1903
Size: 34 3698 34 3678
Fragments arranged by size:
3698 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5336 6546 5336
Size: 1210 6234
BclI T'GATC_A
Cuts at: 1548 3424 3783 1548
Size: 1876 359 5209
Fragments arranged by size:
5209 1876 359
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6055 136
Size: 107 122 71 631 605 4383 1525
Fragments arranged by size:
4383 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7439 7439
Size: 7444
BlpI GC'TnA_GC
Cuts at: 4861 4861
Size: 7444
Bme1580I G_kGCm'C
Cuts at: 1790 2181 4164 4183 4449 5505 6661 1790
Size: 391 1983 19 266 1056 1156 2573
Fragments arranged by size:
2573 1983 1156 1056 391 266 19
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7444
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5175 6133 442
Size: 416 53 83 399 24 3758 958 1753
Fragments arranged by size:
3758 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7444
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3778 3778
Size: 7444
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3381 4400 6104 1239
Size: 2142 1019 1704 2579
Fragments arranged by size:
2579 2142 1704 1019
Bpu10I CC'TnA_GC
Cuts at: 3657 3657
Size: 7444
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3255 5569 6434 6732 3255
Size: 2314 865 298 3967
Fragments arranged by size:
3967 2314 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4497 4632 6107 882
Size: 617 2998 135 1475 2219
Fragments arranged by size:
2998 2219 1475 617 135
BsaAI yAC'GTr
Cuts at: 493 1204 2021 3249 4105 5235 493
Size: 711 817 1228 856 1130 2702
Fragments arranged by size:
2702 1228 1130 856 817 711
BsaBI GATnn'nnATC
Cuts at: 3076 3539 3737 7438 3076
Size: 463 198 3701 3082
Fragments arranged by size:
3701 3082 463 198
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3480 4293 5251 5633
Size: 53 83 186 936 1947 813 958 382
Cuts at: 5633 6268 275
Size: 635 1451
Fragments arranged by size:
1947 1451 958 936 813 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3874 4550 5877 6275 6549 6696 7107 3874
Size: 676 1327 398 274 147 411 4211
Fragments arranged by size:
4211 1327 676 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7219
Fragments arranged by size:
7219 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3600 3805 4447 1186
Size: 2414 205 642 4183
Fragments arranged by size:
4183 2414 642 205
BseYI C'CCAG_C
Cuts at: 3058 6647 7143 3058
Size: 3589 496 3359
Fragments arranged by size:
3589 3359 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2651 2669 4324 4679 1163
Size: 573 915 18 1655 355 3928
Fragments arranged by size:
3928 1655 915 573 355 18
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4894 5655 6683 1540
Size: 275 186 2893 761 1028 2301
Fragments arranged by size:
2893 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2181 2916 3090 4183 5505 5590 6661 2181
Size: 735 174 1093 1322 85 1071 2964
Fragments arranged by size:
2964 1322 1093 1071 735 174 85
BsmI GAATG_Cn'
Cuts at: 3651 4955 5048 3651
Size: 1304 93 6047
Fragments arranged by size:
6047 1304 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2181 2916 2990 3090 3260 3470 4164
Size: 399 391 735 74 100 170 210 694
Cuts at: 4164 4183 4449 4515 4763 5505 5590 6661 1391
Size: 19 266 66 248 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 735 694 399 391 266
248 210 170 100 85 74 66 19
BspEI T'CCGG_A
Cuts at: 3874 7107 3874
Size: 3233 4211
BspHI T'CATG_A
Cuts at: 4296 5333 7063 4296
Size: 1037 1730 4677
Fragments arranged by size:
4677 1730 1037
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2432 3646 3656 4183 4608 4799 844
Size: 1588 1214 10 527 425 191 3489
Fragments arranged by size:
3489 1588 1214 527 425 191 10
BsrBI CCG'CTC
Cuts at: 1394 1823 3804 5331 1394
Size: 429 1981 1527 3507
Fragments arranged by size:
3507 1981 1527 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5939 6113 65
Size: 1183 4691 174 1396
Fragments arranged by size:
4691 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4550 6088 6275 1700
Size: 110 2740 1538 187 2869
Fragments arranged by size:
2869 2740 1538 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7444
BssHII G'CGCG_C
Cuts at: 1925 4645 1925
Size: 2720 4724
BssSI C'ACGA_G
Cuts at: 5504 6516 5504
Size: 1012 6432
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7444
BstBI TT'CG_AA
Cuts at: 6248 6248
Size: 7444
BstEII G'GTnAC_C
Cuts at: 1184 3454 1184
Size: 2270 5174
BstYI r'GATC_y
Cuts at: 1972 2680 3071 3899 4282 4735 4774 5141 5526
Size: 708 391 828 383 453 39 367 385
Cuts at: 5526 5543 6995 7081 7093 7439 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 828 708 453 391 385 383
367 346 86 39 17 12
Bsu36I CC'TnA_GG
Cuts at: 2949 2949
Size: 7444
BtgI C'CryG_G
Cuts at: 513 1065 2238 2718 3903 4303 4438 513
Size: 552 1173 480 1185 400 135 3519
Fragments arranged by size:
3519 1185 1173 552 480 400 135
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4132 4330 487
Size: 683 56 2906 198 3601
Fragments arranged by size:
3601 2906 683 198 56
BtsI GCAGTG_nn'
Cuts at: 2351 2448 4525 4537 4960 5754 5774 2351
Size: 97 2077 12 423 794 20 4021
Fragments arranged by size:
4021 2077 794 423 97 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7444
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7409
DraI TTT'AAA
Cuts at: 2134 4748 2134
Size: 2614 4830
DraIII CAC_nnn'GTG
Cuts at: 3454 4405 4444 3454
Size: 951 39 6454
Fragments arranged by size:
6454 951 39
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3361 6451 809
Size: 507 2045 3090 1802
Fragments arranged by size:
3090 2045 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2155 3694 3984
Size: 54 1425 50 275 18 325 1539 290
Cuts at: 3984 4277 4891 5780 7409 8
Size: 293 614 889 1629 43
Fragments arranged by size:
1629 1539 1425 889 614 325 293 290
275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4891 1537
Size: 275 3079 4090
Fragments arranged by size:
4090 3079 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3190 5374 1353
Size: 1837 2184 3423
Fragments arranged by size:
3423 2184 1837
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4270 6031 6405 6551 387
Size: 1064 138 2681 1761 374 146 1280
Fragments arranged by size:
2681 1761 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2414 3306 3381 3846 4400 5507 6104
Size: 393 782 892 75 465 554 1107 597
Cuts at: 6104 6891 7159 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1107 892 787 782 597 554 465
393 268 75
EcoO109I rG'GnC_Cy
Cuts at: 3922 4160 4161 3922
Size: 238 1 7205
Fragments arranged by size:
7205 238 1
EcoRI G'AATT_C
Cuts at: 4753 5131 4753
Size: 378 7066
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4715 4715
Size: 7444
FspI TGC'GCA
Cuts at: 5950 5950
Size: 7444
HaeII r_GCGC'y
Cuts at: 1678 6591 1678
Size: 4913 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2073 2105 2219 2251 3308
Size: 32 135 32 501 32 114 32 1057
Cuts at: 3308 3340 3806 3838 4249 4281 4442 4474 6085
Size: 32 466 32 411 32 161 32 1611
Cuts at: 6085 6117 6159 6191 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1611 1057 501 466 411 161 135
114 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2421 4233 4788 5034 669
Size: 988 281 483 1812 555 246 3079
Fragments arranged by size:
3079 1812 988 555 483 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7444
HpaI GTT'AAC
Cuts at: 2421 5034 2421
Size: 2613 4831
KpnI G_GTAC'C
Cuts at: 4769 4769
Size: 7444
MfeI C'AATT_G
Cuts at: 5043 5043
Size: 7444
MluI A'CGCG_T
Cuts at: 7320 7320
Size: 7444
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4485 6558 6742 1305
Size: 3180 2073 184 2007
Fragments arranged by size:
3180 2073 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2157 3986 4279 10
Size: 54 1425 343 325 1829 293 3175
Fragments arranged by size:
3175 1829 1425 343 325 293 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2190 3717 4176 4226 4530 4655 5402
Size: 648 1024 1527 459 50 304 125 747
Cuts at: 5402 5761 5920 518
Size: 359 159 2042
Fragments arranged by size:
2042 1527 1024 747 648 459 359 304
159 125 50
MspA1I CmG'CkG
Cuts at: 1606 2720 4860 5537 6685 6930 1606
Size: 1114 2140 677 1148 245 2120
Fragments arranged by size:
2140 2120 1148 1114 677 245
NcoI C'CATG_G
Cuts at: 513 1065 3903 513
Size: 552 2838 4054
Fragments arranged by size:
4054 2838 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7444
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7444
NotI GC'GGCC_GC
Cuts at: 4891 4891
Size: 7444
NspI r_CATG'y
Cuts at: 1132 1229 2869 4802 6347 1132
Size: 97 1640 1933 1545 2229
Fragments arranged by size:
2229 1933 1640 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7444
PciI A'CATG_T
Cuts at: 6343 6343
Size: 7444
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4732 1108
Size: 3624 3820
PmeI GTTT'AAAC
Cuts at: 4748 4748
Size: 7444
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5513 5545 7026 7058 5513
Size: 32 1481 32 5899
Fragments arranged by size:
5899 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7444
PsiI TTA'TAA
Cuts at: 5014 5014
Size: 7444
PspOMI G'GGCC_C
Cuts at: 4160 4160
Size: 7444
PstI C_TGCA'G
Cuts at: 830 2358 3193 4532 4796 830
Size: 1528 835 1339 264 3478
Fragments arranged by size:
3478 1528 1339 835 264
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7444
SacII CC_GC'GG
Cuts at: 2721 2721
Size: 7444
SalI G'TCGA_C
Cuts at: 1936 4786 1936
Size: 2850 4594
SbfI CC_TGCA'GG
Cuts at: 4796 4796
Size: 7444
ScaI AGT'ACT
Cuts at: 2975 3491 4274 5692 7404 2975
Size: 516 783 1418 1712 3015
Fragments arranged by size:
3015 1712 1418 783 516
SfcI C'TryA_G
Cuts at: 826 1045 2354 3189 3964 4528 4792 5927 6608
Size: 219 1309 835 775 564 264 1135 681
Cuts at: 6608 6799 826
Size: 191 1471
Fragments arranged by size:
1471 1309 1135 835 775 681 564 264
219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7444
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7444
SmaI CCC'GGG
Cuts at: 1872 4061 4771 1872
Size: 2189 710 4545
Fragments arranged by size:
4545 2189 710
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3270 3578 5548 6449 6711 820
Size: 197 926 1327 308 1970 901 262 1553
Fragments arranged by size:
1970 1553 1327 926 901 308 262 197
SnaBI TAC'GTA
Cuts at: 493 5235 493
Size: 4742 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7444
SphI G_CATG'C
Cuts at: 1132 4802 1132
Size: 3670 3774
SspI AAT'ATT
Cuts at: 5 52 2210 3023 5368 5
Size: 47 2158 813 2345 2081
Fragments arranged by size:
2345 2158 2081 813 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7444
StyI C'CwwG_G
Cuts at: 513 1065 2765 3903 4883 513
Size: 552 1700 1138 980 3074
Fragments arranged by size:
3074 1700 1138 980 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2018 2180 2758 4397 5471 5488 5641
Size: 1255 438 162 578 1639 1074 17 153
Cuts at: 5641 5826 6271 325
Size: 185 445 1498
Fragments arranged by size:
1639 1498 1255 1074 578 445 438 185
162 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2973 3489 4143
Size: 274 80 33 51 1443 996 516 654
Cuts at: 4143 4272 4674 5690 7402 96
Size: 129 402 1016 1712 138
Fragments arranged by size:
1712 1443 1016 996 654 516 402 274
138 129 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2890 2922 3637 3669 7404 7436 2890
Size: 32 715 32 3735 32 2898
Fragments arranged by size:
3735 2898 715 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7444
XbaI T'CTAG_A
Cuts at: 4780 4780
Size: 7444
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2111 4612 2111
Size: 2501 4943
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7444
XmaI C'CCGG_G
Cuts at: 1870 4059 4769 1870
Size: 2189 710 4545
Fragments arranged by size:
4545 2189 710
XmnI GAAnn'nnTTC
Cuts at: 2713 2804 4695 5573 2713
Size: 91 1891 878 4584
Fragments arranged by size:
4584 1891 878 91
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4294 5252 276
Size: 53 83 186 936 2760 958 2468
Fragments arranged by size:
2760 2468 958 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI SacII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI
SanDI SapI SexAI SfiI SfoI SrfI SwaI