Restriction map for
pFN21AE5813
(Circular) (Six-base) MAPSORT of: pFN21AE5813.seq Check: 4326 from: 1 to: 7483
pFN21AE5813 7483 bp
With 182 enzymes: SgfI *
December 8, 2014 13:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5293 278
Size: 53 83 186 936 3757 2468
Fragments arranged by size:
3757 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4826 1937
Size: 2889 4594
Acc65I G'GTAC_C
Cuts at: 2763 4804 2763
Size: 2041 5442
AclI AA'CG_TT
Cuts at: 1563 5610 5983 1563
Size: 4047 373 3063
Fragments arranged by size:
4047 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2118 2133 2339 2595 2769 3269 4208 5546
Size: 486 15 206 256 174 500 939 1338
Cuts at: 5546 6930 7198 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1338 939 500 486 268 256
206 174 15
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7286
AflIII A'CryG_T
Cuts at: 1203 2341 6382 7359 1203
Size: 1138 4041 977 1327
Fragments arranged by size:
4041 1327 1138 977
AgeI A'CCGG_T
Cuts at: 6314 6314
Size: 7483
AhdI GACnn_n'nnGTC
Cuts at: 6212 6212
Size: 7483
AleI CACnn'nnGTG
Cuts at: 1166 3777 1166
Size: 2611 4872
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4730 1184
Size: 3546 3937
AlwNI CAG_nnn'CTG
Cuts at: 3244 3967 4112 4246 5226 6798 3244
Size: 723 145 134 980 1572 3929
Fragments arranged by size:
3929 1572 980 723 145 134
ApaI G_GGCC'C
Cuts at: 2830 2830
Size: 7483
ApaLI G'TGCA_C
Cuts at: 2201 2519 5540 6696 2201
Size: 318 3021 1156 2988
Fragments arranged by size:
3021 2988 1156 318
ApoI r'AATT_y
Cuts at: 1493 2426 2650 2724 3389 3956 4792 5018 5170
Size: 933 224 74 665 567 836 226 152
Cuts at: 5170 7279 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 933 836 665 567 226 224
152 74
AseI AT'TA_AT
Cuts at: 160 1032 6037 160
Size: 872 5005 1606
Fragments arranged by size:
5005 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7483
AvaI C'yCGr_G
Cuts at: 1870 1943 2816 4250 4808 1870
Size: 73 873 1434 558 4545
Fragments arranged by size:
4545 1434 873 558 73
AvrII C'CTAG_G
Cuts at: 4273 4273
Size: 7483
BamHI G'GATC_C
Cuts at: 4090 4813 5180 4090
Size: 723 367 6393
Fragments arranged by size:
6393 723 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2470 2763 3133 4181 4804
Size: 325 217 627 683 293 370 1048 623
Cuts at: 4804 6259 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1048 683 627 623 370 325
293 217
BanII G_rGCy'C
Cuts at: 1391 2484 2816 2830 3051 3827 4802 1391
Size: 1093 332 14 221 776 975 4072
Fragments arranged by size:
4072 1093 975 776 332 221 14
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2583 3113 3630 928
Size: 971 684 530 517 4781
Fragments arranged by size:
4781 971 684 530 517
BbvCI CC'TCA_GC
Cuts at: 4051 4051
Size: 7483
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3689 3723 5674 5708 1903
Size: 34 1752 34 1951 34 3678
Fragments arranged by size:
3678 1951 1752 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3264 5375 6585 3264
Size: 2111 1210 4162
Fragments arranged by size:
4162 2111 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7483
BfrBI ATG'CAT
Cuts at: 4707 4707
Size: 7483
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3746 6094 136
Size: 107 122 71 631 605 2074 2348 1525
Fragments arranged by size:
2348 2074 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7478 7478
Size: 7483
BlpI GC'TnA_GC
Cuts at: 3696 4900 3696
Size: 1204 6279
Bme1580I G_kGCm'C
Cuts at: 1790 2205 2523 2830 5544 6700 1790
Size: 415 318 307 2714 1156 2573
Fragments arranged by size:
2714 2573 1156 415 318 307
BmgBI CAC'GTC
Cuts at: 1318 2986 1318
Size: 1668 5815
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3814 5214 6172
Size: 416 53 83 399 24 2397 1400 958
Cuts at: 6172 442
Size: 1753
Fragments arranged by size:
2397 1753 1400 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7483
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2397 4452 2397
Size: 2055 5428
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2221 2864 3137 4134 4140 4216 6143 1239
Size: 982 643 273 997 6 76 1927 2579
Fragments arranged by size:
2579 1927 997 982 643 273 76 6
Bpu10I CC'TnA_GC
Cuts at: 2810 3657 4051 4236 2810
Size: 847 394 185 6057
Fragments arranged by size:
6057 847 394 185
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5608 6473 6771 5608
Size: 865 298 6320
Fragments arranged by size:
6320 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3020 4552 6146 882
Size: 617 1521 1532 1594 2219
Fragments arranged by size:
2219 1594 1532 1521 617
BsaAI yAC'GTr
Cuts at: 493 1204 2200 5274 493
Size: 711 996 3074 2702
Fragments arranged by size:
3074 2702 996 711
BsaBI GATnn'nnATC
Cuts at: 7477 7477
Size: 7483
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2970 5290 5672 6307
Size: 53 83 186 936 1437 2320 382 635
Cuts at: 6307 275
Size: 1451
Fragments arranged by size:
2320 1451 1437 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2680 2766 5916 6314 6588 6735 7146 2680
Size: 86 3150 398 274 147 411 3017
Fragments arranged by size:
3150 3017 411 398 274 147 86
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2788 2818 4640 4670 1178
Size: 30 165 30 1385 30 1822 30 3991
Fragments arranged by size:
3991 1822 1385 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2024 2528 4010 1186
Size: 838 504 1482 4659
Fragments arranged by size:
4659 1482 838 504
BseYI C'CCAG_C
Cuts at: 2443 2561 2908 2998 4038 4043 4404 4662 6686
Size: 118 347 90 1040 5 361 258 2024
Cuts at: 6686 7182 2443
Size: 496 2744
Fragments arranged by size:
2744 2024 1040 496 361 347 258 118
90 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2265 2647 2724 4559 1163
Size: 573 529 382 77 1835 4087
Fragments arranged by size:
4087 1835 573 529 382 77
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4933 5694 6722 1540
Size: 275 186 2932 761 1028 2301
Fragments arranged by size:
2932 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2205 2523 2933 3051 3150 5544 5629 6700 2205
Size: 318 410 118 99 2394 85 1071 2988
Fragments arranged by size:
2988 2394 1071 410 318 118 99 85
BsiWI C'GTAC_G
Cuts at: 2344 2344
Size: 7483
BsmI GAATG_Cn'
Cuts at: 4994 5087 4994
Size: 93 7390
BsmBI CGTCTCn'nnnn_
Cuts at: 2594 2594
Size: 7483
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2205 2484 2523 2816 2830 2933 3051
Size: 399 415 279 39 293 14 103 118
Cuts at: 3051 3150 3827 4802 5544 5629 6700 1391
Size: 99 677 975 742 85 1071 2174
Fragments arranged by size:
2174 1071 975 742 677 415 399 293
279 118 103 99 85 39 14
BspEI T'CCGG_A
Cuts at: 7146 7146
Size: 7483
BspHI T'CATG_A
Cuts at: 2026 5372 7102 2026
Size: 3346 1730 2407
Fragments arranged by size:
3346 2407 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2592 4722 4838 844
Size: 1748 2130 116 3489
Fragments arranged by size:
3489 2130 1748 116
BsrBI CCG'CTC
Cuts at: 1394 1823 2860 5370 1394
Size: 429 1037 2510 3507
Fragments arranged by size:
3507 2510 1037 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5978 6152 65
Size: 1183 4730 174 1396
Fragments arranged by size:
4730 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3009 4619 4635 6127 6314 1700
Size: 110 1199 1610 16 1492 187 2869
Fragments arranged by size:
2869 1610 1492 1199 187 110 16
BsrGI T'GTAC_A
Cuts at: 96 2952 4656 96
Size: 2856 1704 2923
Fragments arranged by size:
2923 2856 1704
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7483
BssSI C'ACGA_G
Cuts at: 5543 6555 5543
Size: 1012 6471
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2290 3244 4246 794
Size: 1496 954 1002 4031
Fragments arranged by size:
4031 1496 1002 954
BstBI TT'CG_AA
Cuts at: 3621 6287 3621
Size: 2666 4817
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7483
BstXI CCAn_nnnn'nTGG
Cuts at: 3326 3685 4264 4366 3326
Size: 359 579 102 6443
Fragments arranged by size:
6443 579 359 102
BstYI r'GATC_y
Cuts at: 1972 4090 4813 5180 5565 5582 7034 7120 7132
Size: 2118 723 367 385 17 1452 86 12
Cuts at: 7132 7478 1972
Size: 346 1977
Fragments arranged by size:
2118 1977 1452 723 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 4376 4376
Size: 7483
BtgI C'CryG_G
Cuts at: 513 1065 2004 3138 3319 3644 3778 4305 4359
Size: 552 939 1134 181 325 134 527 54
Cuts at: 4359 513
Size: 3637
Fragments arranged by size:
3637 1134 939 552 527 325 181 134
54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4332 487
Size: 683 56 3106 3638
Fragments arranged by size:
3638 3106 683 56
BtsI GCAGTG_nn'
Cuts at: 3242 3637 3772 4474 4676 4999 5793 5813 3242
Size: 395 135 702 202 323 794 20 4912
Fragments arranged by size:
4912 794 702 395 323 202 135 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7483
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7448
DraI TTT'AAA
Cuts at: 4787 4787
Size: 7483
DraIII CAC_nnn'GTG
Cuts at: 3467 3467
Size: 7483
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6490 809
Size: 507 5174 1802
Fragments arranged by size:
5174 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2550 3328 4554
Size: 54 1425 50 275 18 720 778 1226
Cuts at: 4554 4611 4699 4930 5819 7448 8
Size: 57 88 231 889 1629 43
Fragments arranged by size:
1629 1425 1226 889 778 720 275 231
88 57 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4930 1537
Size: 275 3118 4090
Fragments arranged by size:
4090 3118 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2152 2505 3413 4443 5413 1353
Size: 799 353 908 1030 970 3423
Fragments arranged by size:
3423 1030 970 908 799 353
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6070 6444 6590 387
Size: 1064 138 4481 374 146 1280
Fragments arranged by size:
4481 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3049 3049
Size: 7483
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2118 2133 2221 2339 2595 2769 2864
Size: 393 486 15 88 118 256 174 95
Cuts at: 2864 3137 3269 4134 4140 4208 4216 5546 6143
Size: 273 132 865 6 68 8 1330 597
Cuts at: 6143 6930 7198 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1330 865 787 597 486 393 273
268 256 174 132 118 95 88 68
15 8 6
EcoNI CCTnn'n_nnAGG
Cuts at: 4687 4775 4687
Size: 88 7395
EcoO109I rG'GnC_Cy
Cuts at: 2826 4519 4729 2826
Size: 1693 210 5580
Fragments arranged by size:
5580 1693 210
EcoRI G'AATT_C
Cuts at: 3956 4792 5170 3956
Size: 836 378 6269
Fragments arranged by size:
6269 836 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4177 4177
Size: 7483
FspI TGC'GCA
Cuts at: 5989 5989
Size: 7483
HaeII r_GCGC'y
Cuts at: 1678 4654 6630 1678
Size: 2976 1976 2531
Fragments arranged by size:
2976 2531 1976
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2363 2395 2675 2707 2940
Size: 32 135 32 791 32 280 32 233
Cuts at: 2940 2972 3333 3365 3485 3517 6124 6156 6198
Size: 32 361 32 120 32 2607 32 42
Cuts at: 6198 6230 1373
Size: 32 2626
Fragments arranged by size:
2626 2607 791 361 280 233 135 120
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4827 5073 669
Size: 988 281 2889 246 3079
Fragments arranged by size:
3079 2889 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3516 748
Size: 2768 4715
HpaI GTT'AAC
Cuts at: 5073 5073
Size: 7483
KpnI G_GTAC'C
Cuts at: 2767 4808 2767
Size: 2041 5442
MfeI C'AATT_G
Cuts at: 3940 5082 3940
Size: 1142 6341
MluI A'CGCG_T
Cuts at: 2341 7359 2341
Size: 5018 2465
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2718 3190 3294 3364 3702 6597 6781 1305
Size: 1413 472 104 70 338 2895 184 2007
Fragments arranged by size:
2895 2007 1413 472 338 184 104 70
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2552 3330 4556 4613 10
Size: 54 1425 343 720 778 1226 57 2880
Fragments arranged by size:
2880 1425 1226 778 720 343 57 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2404 3143 3200 3324 3611 3719 3777
Size: 648 1238 739 57 124 287 108 58
Cuts at: 3777 3896 4364 4468 4496 5441 5800 5959 518
Size: 119 468 104 28 945 359 159 2042
Fragments arranged by size:
2042 1238 945 739 648 468 359 287
159 124 119 108 104 58 57 28
MspA1I CmG'CkG
Cuts at: 1606 2287 4055 4662 4899 5576 6724 6969 1606
Size: 681 1768 607 237 677 1148 245 2120
Fragments arranged by size:
2120 1768 1148 681 677 607 245 237
NaeI GCC'GGC
Cuts at: 3011 3011
Size: 7483
NcoI C'CATG_G
Cuts at: 513 1065 2004 3138 3644 513
Size: 552 939 1134 506 4352
Fragments arranged by size:
4352 1134 939 552 506
NdeI CA'TA_TG
Cuts at: 387 3298 387
Size: 2911 4572
NgoMIV G'CCGG_C
Cuts at: 3009 3009
Size: 7483
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7483
NotI GC'GGCC_GC
Cuts at: 4930 4930
Size: 7483
NsiI A_TGCA'T
Cuts at: 4709 4709
Size: 7483
NspI r_CATG'y
Cuts at: 1132 1229 3542 3610 4333 4841 6386 1132
Size: 97 2313 68 723 508 1545 2229
Fragments arranged by size:
2313 2229 1545 723 508 97 68
PasI CC'CwG_GG
Cuts at: 1348 2875 1348
Size: 1527 5956
PciI A'CATG_T
Cuts at: 6382 6382
Size: 7483
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3421 1108
Size: 2313 5170
PfoI T'CCnGG_A
Cuts at: 3796 4093 3796
Size: 297 7186
PmeI GTTT'AAAC
Cuts at: 4787 4787
Size: 7483
PmlI CAC'GTG
Cuts at: 2200 2200
Size: 7483
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5552 5584 7065 7097 5552
Size: 32 1481 32 5938
Fragments arranged by size:
5938 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4519 4519
Size: 7483
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7483
PsiI TTA'TAA
Cuts at: 5053 5053
Size: 7483
PspOMI G'GGCC_C
Cuts at: 2826 2826
Size: 7483
PstI C_TGCA'G
Cuts at: 830 2304 2356 3240 3632 4011 4835 830
Size: 1474 52 884 392 379 824 3478
Fragments arranged by size:
3478 1474 884 824 392 379 52
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7483
PvuII CAG'CTG
Cuts at: 2287 4055 4662 2287
Size: 1768 607 5108
Fragments arranged by size:
5108 1768 607
SacI G_AGCT'C
Cuts at: 3051 3051
Size: 7483
SalI G'TCGA_C
Cuts at: 1936 4825 1936
Size: 2889 4594
SbfI CC_TGCA'GG
Cuts at: 4835 4835
Size: 7483
ScaI AGT'ACT
Cuts at: 5731 7443 5731
Size: 1712 5771
SexAI A'CCwGG_T
Cuts at: 2544 3666 2544
Size: 1122 6361
SfcI C'TryA_G
Cuts at: 826 1045 2300 2352 3236 3628 4007 4831 5966
Size: 219 1255 52 884 392 379 824 1135
Cuts at: 5966 6647 6838 826
Size: 681 191 1471
Fragments arranged by size:
1471 1255 1135 884 824 681 392 379
219 191 52
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7483
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7483
SmaI CCC'GGG
Cuts at: 1872 2818 4810 1872
Size: 946 1992 4545
Fragments arranged by size:
4545 1992 946
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5587 6488 6750 820
Size: 197 926 3644 901 262 1553
Fragments arranged by size:
3644 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5274 493
Size: 4781 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7483
SphI G_CATG'C
Cuts at: 1132 4841 1132
Size: 3709 3774
SspI AAT'ATT
Cuts at: 5 52 5407 5
Size: 47 5355 2081
Fragments arranged by size:
5355 2081 47
StuI AGG'CCT
Cuts at: 1517 2457 1517
Size: 940 6543
StyI C'CwwG_G
Cuts at: 513 1065 2004 3122 3138 3644 3990 4017 4212
Size: 552 939 1118 16 506 346 27 195
Cuts at: 4212 4273 4539 4922 513
Size: 61 266 383 3074
Fragments arranged by size:
3074 1118 939 552 506 383 346 266
195 61 27 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2768 3151 4405 4654 4658 5510 5527
Size: 1255 1188 383 1254 249 4 852 17
Cuts at: 5527 5680 5865 6310 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1254 1188 852 445 383 249
185 153 17 4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2952 3285 3702
Size: 274 80 33 51 1443 975 333 417
Cuts at: 3702 4656 5729 7441 96
Size: 954 1073 1712 138
Fragments arranged by size:
1712 1443 1073 975 954 417 333 274
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2788 2820 3425 3457 4384 4416 7443 7475 2788
Size: 32 605 32 927 32 3027 32 2796
Fragments arranged by size:
3027 2796 927 605 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3935 1316
Size: 2619 4864
XbaI T'CTAG_A
Cuts at: 2308 4819 2308
Size: 2511 4972
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4594 4594
Size: 7483
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7483
XmaI C'CCGG_G
Cuts at: 1870 2816 4808 1870
Size: 946 1992 4545
Fragments arranged by size:
4545 1992 946
XmnI GAAnn'nnTTC
Cuts at: 5612 5612
Size: 7483
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5291 276
Size: 53 83 186 936 3757 2468
Fragments arranged by size:
3757 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BstZ17I EcoRV
FseI FspAI KasI NarI NruI PacI PspXI PsrI
RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI