Restriction map for
pFN21AE5810
(Circular) (Six-base) MAPSORT of: pFN21AE5810.seq Check: 7628 from: 1 to: 9070
pFN21AE5810 9070 bp
With 182 enzymes: SgfI *
December 8, 2014 13:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3463 3463
Size: 9070
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2694 4178 6880 278
Size: 53 83 186 936 1158 1484 2702 2468
Fragments arranged by size:
2702 2468 1484 1158 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5460 6413 1937
Size: 3523 953 4594
Fragments arranged by size:
4594 3523 953
Acc65I G'GTAC_C
Cuts at: 3465 6391 3465
Size: 2926 6144
AclI AA'CG_TT
Cuts at: 1563 7197 7570 1563
Size: 5634 373 3063
Fragments arranged by size:
5634 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2620 2947 2972 3632 3845 3909 4848 4896
Size: 988 327 25 660 213 64 939 48
Cuts at: 4896 5195 5392 7133 8517 8785 1632
Size: 299 197 1741 1384 268 1917
Fragments arranged by size:
1917 1741 1384 988 939 660 327 299
268 213 197 64 48 25
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8873
AflIII A'CryG_T
Cuts at: 1203 3741 4270 4342 6081 6253 7969 8946 1203
Size: 2538 529 72 1739 172 1716 977 1327
Fragments arranged by size:
2538 1739 1716 1327 977 529 172 72
AgeI A'CCGG_T
Cuts at: 7901 7901
Size: 9070
AhdI GACnn_n'nnGTC
Cuts at: 7799 7799
Size: 9070
AleI CACnn'nnGTG
Cuts at: 1166 4377 5373 5865 1166
Size: 3211 996 492 4371
Fragments arranged by size:
4371 3211 996 492
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4737 1184
Size: 3553 5517
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4478 4510 4478
Size: 32 9038
AlwNI CAG_nnn'CTG
Cuts at: 2173 3220 4192 4850 5179 5687 6813 8385 2173
Size: 1047 972 658 329 508 1126 1572 2858
Fragments arranged by size:
2858 1572 1126 1047 972 658 508 329
ApaI G_GGCC'C
Cuts at: 3329 4325 3329
Size: 996 8074
ApaLI G'TGCA_C
Cuts at: 3476 4370 7127 8283 3476
Size: 894 2757 1156 4263
Fragments arranged by size:
4263 2757 1156 894
ApoI r'AATT_y
Cuts at: 1493 4508 4818 5011 6379 6605 6757 8866 1493
Size: 3015 310 193 1368 226 152 2109 1697
Fragments arranged by size:
3015 2109 1697 1368 310 226 193 152
AseI AT'TA_AT
Cuts at: 160 1032 7624 160
Size: 872 6592 1606
Fragments arranged by size:
6592 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9070
AvaI C'yCGr_G
Cuts at: 1870 1943 2035 3774 4324 5586 5879 6395 1870
Size: 73 92 1739 550 1262 293 516 4545
Fragments arranged by size:
4545 1739 1262 550 516 293 92 73
BamHI G'GATC_C
Cuts at: 5244 6400 6767 5244
Size: 1156 367 7547
Fragments arranged by size:
7547 1156 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3465 5375 6231 6391 7846
Size: 325 217 627 1678 1910 856 160 1455
Cuts at: 7846 618
Size: 1842
Fragments arranged by size:
1910 1842 1678 1455 856 627 325 217
160
BanII G_rGCy'C
Cuts at: 1391 3329 3633 3951 4325 5579 6162 6389 1391
Size: 1938 304 318 374 1254 583 227 4072
Fragments arranged by size:
4072 1938 1254 583 374 318 304 227
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2433 3234 3409 6008 6042 928
Size: 971 534 801 175 2599 34 3956
Fragments arranged by size:
3956 2599 971 801 534 175 34
BbvCI CC'TCA_GC
Cuts at: 3942 3942
Size: 9070
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2597 2631 7261 7295 1903
Size: 34 660 34 4630 34 3678
Fragments arranged by size:
4630 3678 660 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6112 6962 8172 6112
Size: 850 1210 7010
Fragments arranged by size:
7010 1210 850
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9070
BfrBI ATG'CAT
Cuts at: 2614 2614
Size: 9070
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7681 136
Size: 107 122 71 631 605 6009 1525
Fragments arranged by size:
6009 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2373 2758 9065 2373
Size: 385 6307 2378
Fragments arranged by size:
6307 2378 385
BlpI GC'TnA_GC
Cuts at: 4115 6487 4115
Size: 2372 6698
Bme1580I G_kGCm'C
Cuts at: 1790 2125 3329 3480 4325 4339 4374 5380 6236
Size: 335 1204 151 845 14 35 1006 856
Cuts at: 6236 7131 8287 1790
Size: 895 1156 2573
Fragments arranged by size:
2573 1204 1156 1006 895 856 845 335
151 35 14
BmgBI CAC'GTC
Cuts at: 1318 4271 1318
Size: 2953 6117
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3241 3692 3983
Size: 416 53 83 399 24 1824 451 291
Cuts at: 3983 4549 6801 7759 442
Size: 566 2252 958 1753
Fragments arranged by size:
2252 1824 1753 958 566 451 416 399
291 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9070
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2887 3346 4742 5668 2887
Size: 459 1396 926 6289
Fragments arranged by size:
6289 1396 926 459
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2370 2874 3341 4157 4189 4572 4702 4829
Size: 1131 504 467 816 32 383 130 127
Cuts at: 4829 5108 5156 5555 6123 6176 6296 7730 1239
Size: 279 48 399 568 53 120 1434 2579
Fragments arranged by size:
2579 1434 1131 816 568 504 467 399
383 279 130 127 120 53 48 32
Bpu10I CC'TnA_GC
Cuts at: 3942 3942
Size: 9070
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2210 2296 3551 4972 6146 7195 8060 8358 2210
Size: 86 1255 1421 1174 1049 865 298 2922
Fragments arranged by size:
2922 1421 1255 1174 1049 865 298 86
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2387 4678 4923 5436 7733 882
Size: 617 888 2291 245 513 2297 2219
Fragments arranged by size:
2297 2291 2219 888 617 513 245
BsaAI yAC'GTr
Cuts at: 493 1204 2563 3742 6256 6861 493
Size: 711 1359 1179 2514 605 2702
Fragments arranged by size:
2702 2514 1359 1179 711 605
BsaBI GATnn'nnATC
Cuts at: 9064 9064
Size: 9070
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2691 4175 6877 7259
Size: 53 83 186 936 1158 1484 2702 382
Cuts at: 7259 7894 275
Size: 635 1451
Fragments arranged by size:
2702 1484 1451 1158 936 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 7503 7901 8175 8322 8733 7503
Size: 398 274 147 411 7840
Fragments arranged by size:
7840 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4260 4290 5232 5262 5348
Size: 30 165 30 2857 30 942 30 86
Cuts at: 5348 5378 1178
Size: 30 4870
Fragments arranged by size:
4870 2857 942 165 86 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2879 3125 3512 3959 4673 5240 5575 5780
Size: 1693 246 387 447 714 567 335 205
Cuts at: 5780 5948 5951 1186
Size: 168 3 4305
Fragments arranged by size:
4305 1693 714 567 447 387 335 246
205 168 3
BseYI C'CCAG_C
Cuts at: 2317 3614 4004 4348 5656 5947 6113 8273 8769
Size: 1297 390 344 1308 291 166 2160 496
Cuts at: 8769 2317
Size: 2618
Fragments arranged by size:
2618 2160 1308 1297 496 390 344 291
166
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2362 3126 3954 4407 4594 5673 5678
Size: 573 626 764 828 453 187 1079 5
Cuts at: 5678 5889 1163
Size: 211 4344
Fragments arranged by size:
4344 1079 828 764 626 573 453 211
187 5
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6520 7281 8309 1540
Size: 275 186 4519 761 1028 2301
Fragments arranged by size:
4519 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3174 3380 3480 3783 4278 4290 4374 4890 5142
Size: 206 100 303 495 12 84 516 252
Cuts at: 5142 6162 7131 7216 8287 3174
Size: 1020 969 85 1071 3957
Fragments arranged by size:
3957 1071 1020 969 516 495 303 252
206 100 85 84 12
BsmI GAATG_Cn'
Cuts at: 2182 2826 3257 6581 6674 2182
Size: 644 431 3324 93 4578
Fragments arranged by size:
4578 3324 644 431 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2125 3174 3329 3380 3480 3633 3783
Size: 399 335 1049 155 51 100 153 150
Cuts at: 3783 3951 4278 4290 4325 4339 4374 4890 5142
Size: 168 327 12 35 14 35 516 252
Cuts at: 5142 5380 5579 6162 6236 6389 7131 7216 8287
Size: 238 199 583 74 153 742 85 1071
Cuts at: 8287 1391
Size: 2174
Fragments arranged by size:
2174 1071 1049 742 583 516 399 335
327 252 238 199 168 155 153 153
150 100 85 74 51 35 35 14
12
BspEI T'CCGG_A
Cuts at: 8733 8733
Size: 9070
BspHI T'CATG_A
Cuts at: 6959 8689 6959
Size: 1730 7340
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2785 3463 3477 4941 5853 6425 844
Size: 1941 678 14 1464 912 572 3489
Fragments arranged by size:
3489 1941 1464 912 678 572 14
BsrBI CCG'CTC
Cuts at: 1394 1823 3516 6957 1394
Size: 429 1693 3441 3507
Fragments arranged by size:
3507 3441 1693 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 7565 7739 65
Size: 1183 6317 174 1396
Fragments arranged by size:
6317 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5553 5613 7714 7901 1700
Size: 110 3743 60 2101 187 2869
Fragments arranged by size:
3743 2869 2101 187 110 60
BsrGI T'GTAC_A
Cuts at: 96 4514 5235 96
Size: 4418 721 3931
Fragments arranged by size:
4418 3931 721
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9070
BssSI C'ACGA_G
Cuts at: 2014 3381 3443 4560 7130 8142 2014
Size: 1367 62 1117 2570 1012 2942
Fragments arranged by size:
2942 2570 1367 1117 1012 62
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2590 2821 3017 3169 3220 4430 794
Size: 1796 231 196 152 51 1210 5434
Fragments arranged by size:
5434 1796 1210 231 196 152 51
BstBI TT'CG_AA
Cuts at: 7874 7874
Size: 9070
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9070
BstYI r'GATC_y
Cuts at: 1972 2373 2758 2944 5244 6400 6767 7152 7169
Size: 401 385 186 2300 1156 367 385 17
Cuts at: 7169 8621 8707 8719 9065 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2300 1977 1452 1156 401 385 385 367
346 186 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 5643 5942 5643
Size: 299 8771
BtgI C'CryG_G
Cuts at: 513 1065 2328 3391 4142 5368 6180 513
Size: 552 1263 1063 751 1226 812 3403
Fragments arranged by size:
3403 1263 1226 1063 812 751 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8331
Fragments arranged by size:
8331 683 56
BtsI GCAGTG_nn'
Cuts at: 2935 3288 4044 4290 5307 5394 5566 5690 6586
Size: 353 756 246 1017 87 172 124 896
Cuts at: 6586 7380 7400 2935
Size: 794 20 4605
Fragments arranged by size:
4605 1017 896 794 756 353 246 172
124 87 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9070
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9035
DraI TTT'AAA
Cuts at: 6374 6374
Size: 9070
DraIII CAC_nnn'GTG
Cuts at: 2407 4430 2407
Size: 2023 7047
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2018 3284 8077 809
Size: 507 702 1266 4793 1802
Fragments arranged by size:
4793 1802 1266 702 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3121 3543 3558
Size: 54 1425 50 275 18 1291 422 15
Cuts at: 3558 4365 5032 5119 5472 5525 6042 6517 7406
Size: 807 667 87 353 53 517 475 889
Cuts at: 7406 9035 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1291 889 807 667 517 475
422 353 275 87 54 53 50 43
18 15
EagI C'GGCC_G
Cuts at: 1537 1812 6517 1537
Size: 275 4705 4090
Fragments arranged by size:
4705 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2639 2948 3651 3821 3885 4253 5888 6048
Size: 1286 309 703 170 64 368 1635 160
Cuts at: 6048 6110 6371 7000 1353
Size: 62 261 629 3423
Fragments arranged by size:
3423 1635 1286 703 629 368 309 261
170 160 64 62
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2020 5243 7657 8031 8177 387
Size: 1064 138 431 3223 2414 374 146 1280
Fragments arranged by size:
3223 2414 1280 1064 431 374 146 138
EcoICRI GAG'CTC
Cuts at: 6160 6160
Size: 9070
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2370 2620 2874 2947 2972 3341 3632
Size: 393 738 250 254 73 25 369 291
Cuts at: 3632 3845 3909 4157 4189 4572 4702 4829 4848
Size: 213 64 248 32 383 130 127 19
Cuts at: 4848 4896 5108 5156 5195 5392 5555 6123 6176
Size: 48 212 48 39 197 163 568 53
Cuts at: 6176 6296 7133 7730 8517 8785 1239
Size: 120 837 597 787 268 1524
Fragments arranged by size:
1524 837 787 738 597 568 393 383
369 291 268 254 250 248 213 212
197 163 130 127 120 73 64 53
48 48 39 32 25 19
EcoNI CCTnn'n_nnAGG
Cuts at: 5384 5849 6350 5384
Size: 465 501 8104
Fragments arranged by size:
8104 501 465
EcoO109I rG'GnC_Cy
Cuts at: 2020 2044 2064 2170 3325 3326 3409 4232 4321
Size: 24 20 106 1155 1 83 823 89
Cuts at: 4321 4415 5413 2020
Size: 94 998 5677
Fragments arranged by size:
5677 1155 998 823 106 94 89 83
24 20 1
EcoRI G'AATT_C
Cuts at: 6379 6757 6379
Size: 378 8692
EcoRV GAT'ATC
Cuts at: 4101 5904 4101
Size: 1803 7267
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2977 3385 6221 2977
Size: 408 2836 5826
Fragments arranged by size:
5826 2836 408
FspI TGC'GCA
Cuts at: 7576 7576
Size: 9070
HaeII r_GCGC'y
Cuts at: 1678 6306 8217 1678
Size: 4628 1911 2531
Fragments arranged by size:
4628 2531 1911
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2294 2326 2616 2648 2974
Size: 32 135 32 722 32 290 32 326
Cuts at: 2974 3006 3046 3078 3521 3553 3590 3593 3622
Size: 32 40 32 443 32 37 3 29
Cuts at: 3622 3625 4007 4039 4216 4248 4260 4292 4373
Size: 3 382 32 177 32 12 32 81
Cuts at: 4373 4405 5497 5529 5621 5653 5663 5695 7711
Size: 32 1092 32 92 32 10 32 2016
Cuts at: 7711 7743 7785 7817 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2016 1092 722 443 382 326 290
177 135 92 81 42 40 37 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 29
12 10 3 3
HincII GTy'rAC
Cuts at: 669 1657 1938 4960 5341 5461 5928 6414 6660
Size: 988 281 3022 381 120 467 486 246
Cuts at: 6660 669
Size: 3079
Fragments arranged by size:
3079 3022 988 486 467 381 281 246
120
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9070
HpaI GTT'AAC
Cuts at: 6660 6660
Size: 9070
KpnI G_GTAC'C
Cuts at: 3469 6395 3469
Size: 2926 6144
MfeI C'AATT_G
Cuts at: 6669 6669
Size: 9070
MluI A'CGCG_T
Cuts at: 8946 8946
Size: 9070
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2034 8184 8368 1305
Size: 729 6150 184 2007
Fragments arranged by size:
6150 2007 729 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3123 3545 5474 10
Size: 54 1425 343 1291 422 1929 3606
Fragments arranged by size:
3606 1929 1425 1291 422 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3671 3707 4157 4377 5373 5865 7028
Size: 648 2505 36 450 220 996 492 1163
Cuts at: 7028 7387 7546 518
Size: 359 159 2042
Fragments arranged by size:
2505 2042 1163 996 648 492 450 359
220 159 36
MspA1I CmG'CkG
Cuts at: 1606 2144 2390 3220 3274 3614 3968 4306 4578
Size: 538 246 830 54 340 354 338 272
Cuts at: 4578 5581 5684 6143 6486 7163 8311 8556 1606
Size: 1003 103 459 343 677 1148 245 2120
Fragments arranged by size:
2120 1148 1003 830 677 538 459 354
343 340 338 272 246 245 103 54
NaeI GCC'GGC
Cuts at: 5615 5615
Size: 9070
NcoI C'CATG_G
Cuts at: 513 1065 3391 513
Size: 552 2326 6192
Fragments arranged by size:
6192 2326 552
NdeI CA'TA_TG
Cuts at: 387 3346 387
Size: 2959 6111
NgoMIV G'CCGG_C
Cuts at: 5613 5613
Size: 9070
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9070
NotI GC'GGCC_GC
Cuts at: 6517 6517
Size: 9070
NsiI A_TGCA'T
Cuts at: 2616 2616
Size: 9070
NspI r_CATG'y
Cuts at: 1132 1229 2614 2618 2687 4346 6071 6085 6428
Size: 97 1385 4 69 1659 1725 14 343
Cuts at: 6428 7973 1132
Size: 1545 2229
Fragments arranged by size:
2229 1725 1659 1545 1385 343 97 69
14 4
PasI CC'CwG_GG
Cuts at: 1348 2048 3913 3952 4236 1348
Size: 700 1865 39 284 6182
Fragments arranged by size:
6182 1865 700 284 39
PciI A'CATG_T
Cuts at: 4342 6081 7969 4342
Size: 1739 1888 5443
Fragments arranged by size:
5443 1888 1739
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 9070
PfoI T'CCnGG_A
Cuts at: 4679 4679
Size: 9070
PmeI GTTT'AAAC
Cuts at: 6374 6374
Size: 9070
PmlI CAC'GTG
Cuts at: 6256 6256
Size: 9070
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2018 2050 7139 7171 8652 8684 2018
Size: 32 5089 32 1481 32 2404
Fragments arranged by size:
5089 2404 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2020 2044 2170 4232 4415 2020
Size: 24 126 2062 183 6675
Fragments arranged by size:
6675 2062 183 126 24
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9070
PsiI TTA'TAA
Cuts at: 6640 6640
Size: 9070
PspOMI G'GGCC_C
Cuts at: 3325 4321 3325
Size: 996 8074
PspXI vC'TCGA_Gb
Cuts at: 3774 5879 3774
Size: 2105 6965
PstI C_TGCA'G
Cuts at: 830 2817 3401 3474 4285 4708 4938 5097 5598
Size: 1987 584 73 811 423 230 159 501
Cuts at: 5598 6219 6422 830
Size: 621 203 3478
Fragments arranged by size:
3478 1987 811 621 584 501 423 230
203 159 73
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9070
PvuII CAG'CTG
Cuts at: 2144 3220 3274 3614 3968 4306 4578 5684 6143
Size: 1076 54 340 354 338 272 1106 459
Cuts at: 6143 2144
Size: 5071
Fragments arranged by size:
5071 1106 1076 459 354 340 338 272
54
SacI G_AGCT'C
Cuts at: 6162 6162
Size: 9070
SalI G'TCGA_C
Cuts at: 1936 5459 6412 1936
Size: 3523 953 4594
Fragments arranged by size:
4594 3523 953
SanDI GG'GwC_CC
Cuts at: 2020 4232 2020
Size: 2212 6858
SapI GCTCTTCn'nnn_
Cuts at: 2639 3651 6110 2639
Size: 1012 2459 5599
Fragments arranged by size:
5599 2459 1012
SbfI CC_TGCA'GG
Cuts at: 3474 4938 6422 3474
Size: 1464 1484 6122
Fragments arranged by size:
6122 1484 1464
ScaI AGT'ACT
Cuts at: 7318 9030 7318
Size: 1712 7358
SexAI A'CCwGG_T
Cuts at: 4426 4426
Size: 9070
SfcI C'TryA_G
Cuts at: 826 1045 2813 3397 3470 3576 4281 4474 4704
Size: 219 1768 584 73 106 705 193 230
Cuts at: 4704 4934 5093 5594 6215 6418 7553 8234 8425
Size: 230 159 501 621 203 1135 681 191
Cuts at: 8425 826
Size: 1471
Fragments arranged by size:
1768 1471 1135 705 681 621 584 501
230 230 219 203 193 191 159 106
73
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9070
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9070
SmaI CCC'GGG
Cuts at: 1872 5588 6397 1872
Size: 3716 809 4545
Fragments arranged by size:
4545 3716 809
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2189 2311 3566 3774 4987 5879
Size: 197 926 246 122 1255 208 1213 892
Cuts at: 5879 6161 7174 8075 8337 820
Size: 282 1013 901 262 1553
Fragments arranged by size:
1553 1255 1213 1013 926 901 892 282
262 246 208 197 122
SnaBI TAC'GTA
Cuts at: 493 6861 493
Size: 6368 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9070
SphI G_CATG'C
Cuts at: 1132 2618 2687 6428 1132
Size: 1486 69 3741 3774
Fragments arranged by size:
3774 3741 1486 69
SspI AAT'ATT
Cuts at: 5 52 6994 5
Size: 47 6942 2081
Fragments arranged by size:
6942 2081 47
StuI AGG'CCT
Cuts at: 1517 2258 3365 3733 3875 4593 6059 1517
Size: 741 1107 368 142 718 1466 4528
Fragments arranged by size:
4528 1466 1107 741 718 368 142
StyI C'CwwG_G
Cuts at: 513 1065 3391 6509 513
Size: 552 2326 3118 3074
Fragments arranged by size:
3118 3074 2326 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3223 3504 4367 5677 7097 7114 7267
Size: 1255 1643 281 863 1310 1420 17 153
Cuts at: 7267 7452 7897 325
Size: 185 445 1498
Fragments arranged by size:
1643 1498 1420 1310 1255 863 445 281
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2809 2857 3855
Size: 274 80 33 51 1443 832 48 998
Cuts at: 3855 4514 5235 6084 7316 9028 96
Size: 659 721 849 1232 1712 138
Fragments arranged by size:
1712 1443 1232 998 849 832 721 659
274 138 80 51 48 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4249 4281 5114 5146 9030 9062 4249
Size: 32 833 32 3884 32 4257
Fragments arranged by size:
4257 3884 833 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9070
XbaI T'CTAG_A
Cuts at: 6406 6406
Size: 9070
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3553 3553
Size: 9070
XhoI C'TCGA_G
Cuts at: 1943 3774 5879 1943
Size: 1831 2105 5134
Fragments arranged by size:
5134 2105 1831
XmaI C'CCGG_G
Cuts at: 1870 5586 6395 1870
Size: 3716 809 4545
Fragments arranged by size:
4545 3716 809
XmnI GAAnn'nnTTC
Cuts at: 2957 4776 7199 2957
Size: 1819 2423 4828
Fragments arranged by size:
4828 2423 1819
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2692 4176 6878 276
Size: 53 83 186 936 1158 1484 2702 2468
Fragments arranged by size:
2702 2468 1484 1158 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BsiWI BsmBI BstXI
BstZ17I FseI FspAI KasI NarI NruI PacI PsrI
RsrII SacII SfiI SfoI SrfI SwaI