Restriction map for
pFN21AE5809
(Circular) (Six-base) MAPSORT of: pFN21AE5809.seq Check: 8754 from: 1 to: 7444
pFN21AE5809 7444 bp
With 182 enzymes: SgfI *
December 8, 2014 13:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5254 278
Size: 53 83 186 936 3718 2468
Fragments arranged by size:
3718 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4787 1937
Size: 2850 4594
Acc65I G'GTAC_C
Cuts at: 4765 4765
Size: 7444
AclI AA'CG_TT
Cuts at: 1563 5571 5944 1563
Size: 4008 373 3063
Fragments arranged by size:
4008 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2457 3260 5507 6891 7159 1632
Size: 825 803 2247 1384 268 1917
Fragments arranged by size:
2247 1917 1384 825 803 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7247
AflIII A'CryG_T
Cuts at: 1203 3107 3365 4525 6343 7320 1203
Size: 1904 258 1160 1818 977 1327
Fragments arranged by size:
1904 1818 1327 1160 977 258
AgeI A'CCGG_T
Cuts at: 6275 6275
Size: 7444
AhdI GACnn_n'nnGTC
Cuts at: 6173 6173
Size: 7444
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7444
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4506 1184
Size: 3322 4122
AlwNI CAG_nnn'CTG
Cuts at: 2098 5187 6759 2098
Size: 3089 1572 2783
Fragments arranged by size:
3089 2783 1572
ApaLI G'TGCA_C
Cuts at: 5501 6657 5501
Size: 1156 6288
ApoI r'AATT_y
Cuts at: 1493 3177 3290 3592 3636 3703 4544 4685 4753
Size: 1684 113 302 44 67 841 141 68
Cuts at: 4753 4979 5131 7240 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1684 841 302 226 152 141
113 68 67 44
AseI AT'TA_AT
Cuts at: 160 1032 4674 5998 160
Size: 872 3642 1324 1606
Fragments arranged by size:
3642 1606 1324 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7444
AvaI C'yCGr_G
Cuts at: 1870 1943 4769 1870
Size: 73 2826 4545
Fragments arranged by size:
4545 2826 73
BamHI G'GATC_C
Cuts at: 4774 5141 4774
Size: 367 7077
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4765 6220 618
Size: 325 217 627 2978 1455 1842
Fragments arranged by size:
2978 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2598 2839 4647 4763 1391
Size: 1207 241 1808 116 4072
Fragments arranged by size:
4072 1808 1207 241 116
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2364 928
Size: 971 465 6008
Fragments arranged by size:
6008 971 465
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5635 5669 1903
Size: 34 3698 34 3678
Fragments arranged by size:
3698 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2556 5336 6546 2556
Size: 2780 1210 3454
Fragments arranged by size:
3454 2780 1210
BclI T'GATC_A
Cuts at: 1548 3054 3073 1548
Size: 1506 19 5919
Fragments arranged by size:
5919 1506 19
BfrBI ATG'CAT
Cuts at: 3500 3500
Size: 7444
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6055 136
Size: 107 122 71 631 605 4383 1525
Fragments arranged by size:
4383 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7439 7439
Size: 7444
BlpI GC'TnA_GC
Cuts at: 2968 4861 2968
Size: 1893 5551
Bme1580I G_kGCm'C
Cuts at: 1790 3065 5505 6661 1790
Size: 1275 2440 1156 2573
Fragments arranged by size:
2573 2440 1275 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7444
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5175 6133 442
Size: 416 53 83 399 24 3758 958 1753
Fragments arranged by size:
3758 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7444
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3834 6104 1239
Size: 2595 2270 2579
Fragments arranged by size:
2595 2579 2270
Bpu10I CC'TnA_GC
Cuts at: 2099 2487 2099
Size: 388 7056
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2363 2653 5569 6434 6732 2363
Size: 290 2916 865 298 3075
Fragments arranged by size:
3075 2916 865 298 290
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2105 3277 6107 882
Size: 617 606 1172 2830 2219
Fragments arranged by size:
2830 2219 1172 617 606
BsaAI yAC'GTr
Cuts at: 493 1204 3108 3727 5235 493
Size: 711 1904 619 1508 2702
Fragments arranged by size:
2702 1904 1508 711 619
BsaBI GATnn'nnATC
Cuts at: 7438 7438
Size: 7444
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3925 5251 5633 6268
Size: 53 83 186 936 2392 1326 382 635
Cuts at: 6268 275
Size: 1451
Fragments arranged by size:
2392 1451 1326 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5877 6275 6549 6696 7107 5877
Size: 398 274 147 411 6214
Fragments arranged by size:
6214 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3384 3414 1178
Size: 30 165 30 1981 30 5208
Fragments arranged by size:
5208 1981 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3522 4074 1186
Size: 2336 552 4556
Fragments arranged by size:
4556 2336 552
BseYI C'CCAG_C
Cuts at: 6647 7143 6647
Size: 496 6948
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2553 4368 1163
Size: 573 817 1815 4239
Fragments arranged by size:
4239 1815 817 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4894 5655 6683 1540
Size: 275 186 2893 761 1028 2301
Fragments arranged by size:
2893 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2598 4647 5505 5590 6661 2598
Size: 2049 858 85 1071 3381
Fragments arranged by size:
3381 2049 1071 858 85
BsmI GAATG_Cn'
Cuts at: 3328 4955 5048 3328
Size: 1627 93 5724
Fragments arranged by size:
5724 1627 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3916 3916
Size: 7444
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2598 2839 3065 4647 4763 5505 5590
Size: 399 808 241 226 1582 116 742 85
Cuts at: 5590 6661 1391
Size: 1071 2174
Fragments arranged by size:
2174 1582 1071 808 742 399 241 226
116 85
BspEI T'CCGG_A
Cuts at: 7107 7107
Size: 7444
BspHI T'CATG_A
Cuts at: 3979 4701 5333 7063 3979
Size: 722 632 1730 4360
Fragments arranged by size:
4360 1730 722 632
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3502 4407 4799 844
Size: 2658 905 392 3489
Fragments arranged by size:
3489 2658 905 392
BsrBI CCG'CTC
Cuts at: 1394 1823 5331 1394
Size: 429 3508 3507
Fragments arranged by size:
3508 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5939 6113 65
Size: 1183 4691 174 1396
Fragments arranged by size:
4691 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6088 6275 1700
Size: 110 4278 187 2869
Fragments arranged by size:
4278 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7444
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7444
BssSI C'ACGA_G
Cuts at: 5504 6516 5504
Size: 1012 6432
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4238 794
Size: 3444 4000
BstBI TT'CG_AA
Cuts at: 6248 6248
Size: 7444
BstEII G'GTnAC_C
Cuts at: 1184 3184 1184
Size: 2000 5444
BstYI r'GATC_y
Cuts at: 1972 2017 2400 4774 5141 5526 5543 6995 7081
Size: 45 383 2374 367 385 17 1452 86
Cuts at: 7081 7093 7439 1972
Size: 12 346 1977
Fragments arranged by size:
2374 1977 1452 385 383 367 346 86
45 17 12
Bsu36I CC'TnA_GG
Cuts at: 2168 2168
Size: 7444
BtgI C'CryG_G
Cuts at: 513 1065 2004 3374 513
Size: 552 939 1370 4583
Fragments arranged by size:
4583 1370 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6705
Fragments arranged by size:
6705 683 56
BtsI GCAGTG_nn'
Cuts at: 3333 4380 4960 5754 5774 3333
Size: 1047 580 794 20 5003
Fragments arranged by size:
5003 1047 794 580 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7444
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7409
DraI TTT'AAA
Cuts at: 3739 4748 3739
Size: 1009 6435
DraIII CAC_nnn'GTG
Cuts at: 2537 3071 2537
Size: 534 6910
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6451 809
Size: 507 5135 1802
Fragments arranged by size:
5135 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2767 4891 5780
Size: 54 1425 50 275 18 937 2124 889
Cuts at: 5780 7409 8
Size: 1629 43
Fragments arranged by size:
2124 1629 1425 937 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4891 1537
Size: 275 3079 4090
Fragments arranged by size:
4090 3079 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2603 3252 3795 4092 5374 1353
Size: 1250 649 543 297 1282 3423
Fragments arranged by size:
3423 1282 1250 649 543 297
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2051 2312 6031 6405 6551 387
Size: 1064 138 462 261 3719 374 146 1280
Fragments arranged by size:
3719 1280 1064 462 374 261 146 138
EcoICRI GAG'CTC
Cuts at: 2596 4645 2596
Size: 2049 5395
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2457 3260 3834 5507 6104 6891 7159
Size: 393 825 803 574 1673 597 787 268
Cuts at: 7159 1239
Size: 1524
Fragments arranged by size:
1673 1524 825 803 787 597 574 393
268
EcoNI CCTnn'n_nnAGG
Cuts at: 4323 4323
Size: 7444
EcoO109I rG'GnC_Cy
Cuts at: 3241 3241
Size: 7444
EcoRI G'AATT_C
Cuts at: 4753 5131 4753
Size: 378 7066
EcoRV GAT'ATC
Cuts at: 3612 4436 3612
Size: 824 6620
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2694 4220 4522 2694
Size: 1526 302 5616
Fragments arranged by size:
5616 1526 302
FspI TGC'GCA
Cuts at: 5950 5950
Size: 7444
HaeII r_GCGC'y
Cuts at: 1678 6591 1678
Size: 4913 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3031 3063 3131 3163 4392
Size: 32 135 32 1459 32 68 32 1229
Cuts at: 4392 4424 6085 6117 6159 6191 1373
Size: 32 1661 32 42 32 2626
Fragments arranged by size:
2626 1661 1459 1229 135 68 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3150 4788 5034 669
Size: 988 281 1212 1638 246 3079
Fragments arranged by size:
3079 1638 1212 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3520 748
Size: 2772 4672
HpaI GTT'AAC
Cuts at: 5034 5034
Size: 7444
KpnI G_GTAC'C
Cuts at: 4769 4769
Size: 7444
MfeI C'AATT_G
Cuts at: 3842 5043 3842
Size: 1201 6243
MluI A'CGCG_T
Cuts at: 3365 7320 3365
Size: 3955 3489
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2328 6558 6742 1305
Size: 1023 4230 184 2007
Fragments arranged by size:
4230 2007 1023 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2769 10
Size: 54 1425 343 937 4685
Fragments arranged by size:
4685 1425 937 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2939 3431 3629 3854 4517 5402 5761
Size: 648 1773 492 198 225 663 885 359
Cuts at: 5761 5920 518
Size: 159 2042
Fragments arranged by size:
2042 1773 885 663 648 492 359 225
198 159
MspA1I CmG'CkG
Cuts at: 1606 2957 3280 4726 4860 5537 6685 6930 1606
Size: 1351 323 1446 134 677 1148 245 2120
Fragments arranged by size:
2120 1446 1351 1148 677 323 245 134
NcoI C'CATG_G
Cuts at: 513 1065 2004 3374 513
Size: 552 939 1370 4583
Fragments arranged by size:
4583 1370 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7444
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7444
NotI GC'GGCC_GC
Cuts at: 4891 4891
Size: 7444
NsiI A_TGCA'T
Cuts at: 3502 3502
Size: 7444
NspI r_CATG'y
Cuts at: 1132 1229 3355 4487 4529 4700 4802 6347 1132
Size: 97 2126 1132 42 171 102 1545 2229
Fragments arranged by size:
2229 2126 1545 1132 171 102 97 42
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7444
PciI A'CATG_T
Cuts at: 4525 6343 4525
Size: 1818 5626
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7444
PfoI T'CCnGG_A
Cuts at: 2022 3760 3811 2022
Size: 1738 51 5655
Fragments arranged by size:
5655 1738 51
PmeI GTTT'AAAC
Cuts at: 4748 4748
Size: 7444
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5513 5545 7026 7058 5513
Size: 32 1481 32 5899
Fragments arranged by size:
5899 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3241 3241
Size: 7444
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7444
PsiI TTA'TAA
Cuts at: 3854 5014 3854
Size: 1160 6284
PstI C_TGCA'G
Cuts at: 830 4796 830
Size: 3966 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7444
PvuII CAG'CTG
Cuts at: 2957 3280 4726 2957
Size: 323 1446 5675
Fragments arranged by size:
5675 1446 323
SacI G_AGCT'C
Cuts at: 2598 4647 2598
Size: 2049 5395
SalI G'TCGA_C
Cuts at: 1936 4786 1936
Size: 2850 4594
SapI GCTCTTCn'nnn_
Cuts at: 2603 2603
Size: 7444
SbfI CC_TGCA'GG
Cuts at: 4796 4796
Size: 7444
ScaI AGT'ACT
Cuts at: 4022 5692 7404 4022
Size: 1670 1712 4062
Fragments arranged by size:
4062 1712 1670
SexAI A'CCwGG_T
Cuts at: 3243 3243
Size: 7444
SfcI C'TryA_G
Cuts at: 826 1045 2958 4161 4792 5927 6608 6799 826
Size: 219 1913 1203 631 1135 681 191 1471
Fragments arranged by size:
1913 1471 1203 1135 681 631 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7444
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7444
SmaI CCC'GGG
Cuts at: 1872 4771 1872
Size: 2899 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2342 2668 5548 6449 6711 820
Size: 197 926 399 326 2880 901 262 1553
Fragments arranged by size:
2880 1553 926 901 399 326 262 197
SnaBI TAC'GTA
Cuts at: 493 3727 5235 493
Size: 3234 1508 2702
Fragments arranged by size:
3234 2702 1508
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7444
SphI G_CATG'C
Cuts at: 1132 4802 1132
Size: 3670 3774
SspI AAT'ATT
Cuts at: 5 52 2561 2774 3168 4108 4460 5368 5
Size: 47 2509 213 394 940 352 908 2081
Fragments arranged by size:
2509 2081 940 908 394 352 213 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7444
StyI C'CwwG_G
Cuts at: 513 1065 2004 2411 3374 3903 4003 4883 513
Size: 552 939 407 963 529 100 880 3074
Fragments arranged by size:
3074 963 939 880 552 529 407 100
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5471 5488 5641 5826 6271 325
Size: 1255 3891 17 153 185 445 1498
Fragments arranged by size:
3891 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2655 4020 5690
Size: 274 80 33 51 1443 678 1365 1670
Cuts at: 5690 7402 96
Size: 1712 138
Fragments arranged by size:
1712 1670 1443 1365 678 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3355 3384 3387 3416 7404 7436 3355
Size: 29 3 29 3988 32 3363
Fragments arranged by size:
3988 3363 32 29 29 3
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7444
XbaI T'CTAG_A
Cuts at: 4780 4780
Size: 7444
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3901 3901
Size: 7444
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7444
XmaI C'CCGG_G
Cuts at: 1870 4769 1870
Size: 2899 4545
XmnI GAAnn'nnTTC
Cuts at: 3683 4675 5573 3683
Size: 992 898 5554
Fragments arranged by size:
5554 992 898
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5252 276
Size: 53 83 186 936 3718 2468
Fragments arranged by size:
3718 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI BsiWI BstXI BstZ17I FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI
PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI