Restriction map for
pFN21AE5807
(Circular) (Six-base) MAPSORT of: pFN21AE5807.seq Check: 779 from: 1 to: 8923
pFN21AE5807 8923 bp
With 182 enzymes: SgfI *
December 8, 2014 13:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6733 278
Size: 53 83 186 936 5197 2468
Fragments arranged by size:
5197 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6266 1937
Size: 4329 4594
Acc65I G'GTAC_C
Cuts at: 3346 6244 3346
Size: 2898 6025
AclI AA'CG_TT
Cuts at: 1563 3380 3538 5604 7050 7423 1563
Size: 1817 158 2066 1446 373 3063
Fragments arranged by size:
3063 2066 1817 1446 373 158
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2112 2490 3053 3212 3266 3528 4650 4982
Size: 480 378 563 159 54 262 1122 332
Cuts at: 4982 5571 6986 8370 8638 1632
Size: 589 1415 1384 268 1917
Fragments arranged by size:
1917 1415 1384 1122 589 563 480 378
332 268 262 159 54
AflII C'TTAA_G
Cuts at: 820 1017 2614 820
Size: 197 1597 7129
Fragments arranged by size:
7129 1597 197
AflIII A'CryG_T
Cuts at: 1203 2074 7822 8799 1203
Size: 871 5748 977 1327
Fragments arranged by size:
5748 1327 977 871
AgeI A'CCGG_T
Cuts at: 7754 7754
Size: 8923
AhdI GACnn_n'nnGTC
Cuts at: 7652 7652
Size: 8923
AleI CACnn'nnGTG
Cuts at: 1166 2429 5852 1166
Size: 1263 3423 4237
Fragments arranged by size:
4237 3423 1263
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2789 3276 1184
Size: 1605 487 6831
Fragments arranged by size:
6831 1605 487
AlwNI CAG_nnn'CTG
Cuts at: 2317 3004 3595 6666 8238 2317
Size: 687 591 3071 1572 3002
Fragments arranged by size:
3071 3002 1572 687 591
ApaI G_GGCC'C
Cuts at: 2063 2063
Size: 8923
ApaLI G'TGCA_C
Cuts at: 5799 6980 8136 5799
Size: 1181 1156 6586
Fragments arranged by size:
6586 1181 1156
ApoI r'AATT_y
Cuts at: 1493 2375 2496 2528 2624 2645 4490 5125 5215
Size: 882 121 32 96 21 1845 635 90
Cuts at: 5215 5360 5465 5482 6232 6458 6610 8719 1493
Size: 145 105 17 750 226 152 2109 1697
Fragments arranged by size:
2109 1845 1697 882 750 635 226 152
145 121 105 96 90 32 21 17
AseI AT'TA_AT
Cuts at: 160 1032 4157 7477 160
Size: 872 3125 3320 1606
Fragments arranged by size:
3320 3125 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8923
AvaI C'yCGr_G
Cuts at: 1870 1943 2119 6248 1870
Size: 73 176 4129 4545
Fragments arranged by size:
4545 4129 176 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5624 5657 5624
Size: 33 8890
BamHI G'GATC_C
Cuts at: 6253 6620 6253
Size: 367 8556
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3346 6244 7699 618
Size: 325 217 627 1559 2898 1455 1842
Fragments arranged by size:
2898 1842 1559 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2063 3566 6242 1391
Size: 672 1503 2676 4072
Fragments arranged by size:
4072 2676 1503 672
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2868 4740 5357 5813 928
Size: 971 969 1872 617 456 4038
Fragments arranged by size:
4038 1872 971 969 617 456
BbvCI CC'TCA_GC
Cuts at: 2243 4583 2243
Size: 2340 6583
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7114 7148 1903
Size: 34 5177 34 3678
Fragments arranged by size:
5177 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5089 5232 6815 8025 5089
Size: 143 1583 1210 5987
Fragments arranged by size:
5987 1583 1210 143
BclI T'GATC_A
Cuts at: 1548 4377 1548
Size: 2829 6094
BfrBI ATG'CAT
Cuts at: 4352 5401 4352
Size: 1049 7874
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 7534 136
Size: 107 122 71 631 605 337 5525 1525
Fragments arranged by size:
5525 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 2419 4004 4448 8918 2419
Size: 1585 444 4470 2424
Fragments arranged by size:
4470 2424 1585 444
BlpI GC'TnA_GC
Cuts at: 2330 6340 2330
Size: 4010 4913
Bme1580I G_kGCm'C
Cuts at: 1790 2063 5803 6984 8140 1790
Size: 273 3740 1181 1156 2573
Fragments arranged by size:
3740 2573 1181 1156 273
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8923
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 6654 7612 442
Size: 416 53 83 399 24 5237 958 1753
Fragments arranged by size:
5237 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8923
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2296 4053 2296
Size: 1757 7166
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2339 2387 2753 2807 4023 4325 7583 1239
Size: 1100 48 366 54 1216 302 3258 2579
Fragments arranged by size:
3258 2579 1216 1100 366 302 54 48
Bpu10I CC'TnA_GC
Cuts at: 2243 4583 2243
Size: 2340 6583
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3300 5663 7048 7913 8211 3300
Size: 2363 1385 865 298 4012
Fragments arranged by size:
4012 2363 1385 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4208 7586 882
Size: 617 2709 3378 2219
Fragments arranged by size:
3378 2709 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2075 6714 493
Size: 711 871 4639 2702
Fragments arranged by size:
4639 2702 871 711
BsaBI GATnn'nnATC
Cuts at: 8917 8917
Size: 8923
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5943 6730 7112 7747
Size: 53 83 186 936 4410 787 382 635
Cuts at: 7747 275
Size: 1451
Fragments arranged by size:
4410 1451 936 787 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 7356 7754 8028 8175 8586 7356
Size: 398 274 147 411 7693
Fragments arranged by size:
7693 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2079 2109 1178
Size: 30 165 30 676 30 7992
Fragments arranged by size:
7992 676 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2231 2303 2360 2750 4045 4342 5051 5672
Size: 1045 72 57 390 1295 297 709 621
Cuts at: 5672 1186
Size: 4437
Fragments arranged by size:
4437 1295 1045 709 621 390 297 72
57
BseYI C'CCAG_C
Cuts at: 2044 2056 3042 4596 5138 8126 8622 2044
Size: 12 986 1554 542 2988 496 2345
Fragments arranged by size:
2988 2345 1554 986 542 496 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2749 1163
Size: 573 1013 7337
Fragments arranged by size:
7337 1013 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6373 7134 8162 1540
Size: 275 186 4372 761 1028 2301
Fragments arranged by size:
4372 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3301 3566 5803 6984 7069 8140 3301
Size: 265 2237 1181 85 1071 4084
Fragments arranged by size:
4084 2237 1181 1071 265 85
BsmI GAATG_Cn'
Cuts at: 4825 5008 5317 6434 6527 4825
Size: 183 309 1117 93 7221
Fragments arranged by size:
7221 1117 309 183 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2405 2405
Size: 8923
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2063 3301 3566 5803 6242 6984 7069
Size: 399 273 1238 265 2237 439 742 85
Cuts at: 7069 8140 1391
Size: 1071 2174
Fragments arranged by size:
2237 2174 1238 1071 742 439 399 273
265 85
BspEI T'CCGG_A
Cuts at: 8586 8586
Size: 8923
BspHI T'CATG_A
Cuts at: 3495 4810 6812 8542 3495
Size: 1315 2002 1730 3876
Fragments arranged by size:
3876 2002 1730 1315
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2781 6278 844
Size: 1937 3497 3489
Fragments arranged by size:
3497 3489 1937
BsrBI CCG'CTC
Cuts at: 1394 1823 6810 1394
Size: 429 4987 3507
Fragments arranged by size:
4987 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2109 4573 5730 5771 5810 7418 7592
Size: 1183 861 2464 1157 41 39 1608 174
Cuts at: 7592 65
Size: 1396
Fragments arranged by size:
2464 1608 1396 1183 1157 861 174 41
39
BsrFI r'CCGG_y
Cuts at: 1700 1810 2129 2199 7567 7754 1700
Size: 110 319 70 5368 187 2869
Fragments arranged by size:
5368 2869 319 187 110 70
BsrGI T'GTAC_A
Cuts at: 96 2689 6096 96
Size: 2593 3407 2923
Fragments arranged by size:
3407 2923 2593
BssHII G'CGCG_C
Cuts at: 1925 2247 1925
Size: 322 8601
BssSI C'ACGA_G
Cuts at: 6983 7995 6983
Size: 1012 7911
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3004 794
Size: 2210 6713
BstBI TT'CG_AA
Cuts at: 7727 7727
Size: 8923
BstEII G'GTnAC_C
Cuts at: 1184 2299 1184
Size: 1115 7808
BstYI r'GATC_y
Cuts at: 1972 2419 3799 4004 4448 5674 6253 6620 7005
Size: 447 1380 205 444 1226 579 367 385
Cuts at: 7005 7022 8474 8560 8572 8918 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1380 1226 579 447 444 385
367 346 205 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4325 4325
Size: 8923
BtgI C'CryG_G
Cuts at: 513 1065 2004 2253 5234 513
Size: 552 939 249 2981 4202
Fragments arranged by size:
4202 2981 939 552 249
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2309 3338 487
Size: 683 56 1083 1029 6072
Fragments arranged by size:
6072 1083 1029 683 56
BtsI GCAGTG_nn'
Cuts at: 3769 4627 6439 7233 7253 3769
Size: 858 1812 794 20 5439
Fragments arranged by size:
5439 1812 858 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8923
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8888
DraI TTT'AAA
Cuts at: 2650 4495 4776 6227 2650
Size: 1845 281 1451 5346
Fragments arranged by size:
5346 1845 1451 281
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7930 809
Size: 507 6614 1802
Fragments arranged by size:
6614 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4431 5865 6370
Size: 54 1425 50 275 18 2601 1434 505
Cuts at: 6370 7259 8888 8
Size: 889 1629 43
Fragments arranged by size:
2601 1629 1434 1425 889 505 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6370 1537
Size: 275 4558 4090
Fragments arranged by size:
4558 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2319 2697 3309 3576 3979 4365 5832 6853
Size: 966 378 612 267 403 386 1467 1021
Cuts at: 6853 1353
Size: 3423
Fragments arranged by size:
3423 1467 1021 966 612 403 386 378
267
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7510 7884 8030 387
Size: 1064 138 5921 374 146 1280
Fragments arranged by size:
5921 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3564 3564
Size: 8923
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2112 2339 2387 2490 2753 2807 3053
Size: 393 480 227 48 103 263 54 246
Cuts at: 3053 3212 3266 3528 4023 4325 4650 4982 5571
Size: 159 54 262 495 302 325 332 589
Cuts at: 5571 6986 7583 8370 8638 1239
Size: 1415 597 787 268 1524
Fragments arranged by size:
1524 1415 787 597 589 495 480 393
332 325 302 268 263 262 246 227
159 103 54 54 48
EcoNI CCTnn'n_nnAGG
Cuts at: 2342 2360 4586 2342
Size: 18 2226 6679
Fragments arranged by size:
6679 2226 18
EcoO109I rG'GnC_Cy
Cuts at: 2060 2067 2207 5788 2060
Size: 7 140 3581 5195
Fragments arranged by size:
5195 3581 140 7
EcoRI G'AATT_C
Cuts at: 2624 6232 6610 2624
Size: 3608 378 4937
Fragments arranged by size:
4937 3608 378
EcoRV GAT'ATC
Cuts at: 5412 5412
Size: 8923
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3296 6140 3296
Size: 2844 6079
FspI TGC'GCA
Cuts at: 2108 7429 2108
Size: 5321 3602
HaeII r_GCGC'y
Cuts at: 1678 8070 1678
Size: 6392 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3784 3816 4109 4141 5171
Size: 32 135 32 2212 32 293 32 1030
Cuts at: 5171 5203 5702 5734 7564 7596 7638 7670 1373
Size: 32 499 32 1830 32 42 32 2626
Fragments arranged by size:
2626 2212 1830 1030 499 293 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4897 6267 6513 669
Size: 988 281 2959 1370 246 3079
Fragments arranged by size:
3079 2959 1370 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3370 5681 748
Size: 2622 2311 3990
Fragments arranged by size:
3990 2622 2311
HpaI GTT'AAC
Cuts at: 6513 6513
Size: 8923
KpnI G_GTAC'C
Cuts at: 3350 6248 3350
Size: 2898 6025
MfeI C'AATT_G
Cuts at: 5291 5577 6522 5291
Size: 286 945 7692
Fragments arranged by size:
7692 945 286
MluI A'CGCG_T
Cuts at: 8799 8799
Size: 8923
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4044 4074 4751 5586 8037 8221 1305
Size: 2739 30 677 835 2451 184 2007
Fragments arranged by size:
2739 2451 2007 835 677 184 30
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4433 5867 10
Size: 54 1425 343 2601 1434 3066
Fragments arranged by size:
3066 2601 1434 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2429 4442 5852 6206 6881 7240 7399
Size: 648 1263 2013 1410 354 675 359 159
Cuts at: 7399 518
Size: 2042
Fragments arranged by size:
2042 2013 1410 1263 675 648 359 354
159
MspA1I CmG'CkG
Cuts at: 1606 2056 2314 2763 3128 6339 7016 8164 8409
Size: 450 258 449 365 3211 677 1148 245
Cuts at: 8409 1606
Size: 2120
Fragments arranged by size:
3211 2120 1148 677 450 449 365 258
245
NaeI GCC'GGC
Cuts at: 2131 2201 2131
Size: 70 8853
NcoI C'CATG_G
Cuts at: 513 1065 2004 2253 513
Size: 552 939 249 7183
Fragments arranged by size:
7183 939 552 249
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8923
NgoMIV G'CCGG_C
Cuts at: 2129 2199 2129
Size: 70 8853
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8923
NotI GC'GGCC_GC
Cuts at: 6370 6370
Size: 8923
NsiI A_TGCA'T
Cuts at: 4354 5403 4354
Size: 1049 7874
NspI r_CATG'y
Cuts at: 1132 1229 6281 7826 1132
Size: 97 5052 1545 2229
Fragments arranged by size:
5052 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8923
PciI A'CATG_T
Cuts at: 7822 7822
Size: 8923
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2078 5977 1108
Size: 970 3899 4054
Fragments arranged by size:
4054 3899 970
PmeI GTTT'AAAC
Cuts at: 6227 6227
Size: 8923
PmlI CAC'GTG
Cuts at: 2075 2075
Size: 8923
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6992 7024 8505 8537 6992
Size: 32 1481 32 7378
Fragments arranged by size:
7378 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2067 5788 2067
Size: 3721 5202
PshAI GACnn'nnGTC
Cuts at: 1613 4313 1613
Size: 2700 6223
PsiI TTA'TAA
Cuts at: 2723 6493 2723
Size: 3770 5153
PspOMI G'GGCC_C
Cuts at: 2059 2059
Size: 8923
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3909 3941 3909
Size: 32 8891
PstI C_TGCA'G
Cuts at: 830 3000 3009 6275 830
Size: 2170 9 3266 3478
Fragments arranged by size:
3478 3266 2170 9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8923
PvuII CAG'CTG
Cuts at: 2056 2314 3128 2056
Size: 258 814 7851
Fragments arranged by size:
7851 814 258
SacI G_AGCT'C
Cuts at: 3566 3566
Size: 8923
SalI G'TCGA_C
Cuts at: 1936 6265 1936
Size: 4329 4594
SapI GCTCTTCn'nnn_
Cuts at: 2319 2697 3576 4365 2319
Size: 378 879 789 6877
Fragments arranged by size:
6877 879 789 378
SbfI CC_TGCA'GG
Cuts at: 6275 6275
Size: 8923
ScaI AGT'ACT
Cuts at: 2550 3099 4036 6156 7171 8883 2550
Size: 549 937 2120 1015 1712 2590
Fragments arranged by size:
2590 2120 1712 1015 937 549
SfcI C'TryA_G
Cuts at: 826 1045 2996 3005 3587 3701 3721 6271 7406
Size: 219 1951 9 582 114 20 2550 1135
Cuts at: 7406 8087 8278 826
Size: 681 191 1471
Fragments arranged by size:
2550 1951 1471 1135 681 582 219 191
114 20 9
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8923
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8923
SmaI CCC'GGG
Cuts at: 1872 6250 1872
Size: 4378 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2614 3315 5678 7027 7928 8190
Size: 197 926 671 701 2363 1349 901 262
Cuts at: 8190 820
Size: 1553
Fragments arranged by size:
2363 1553 1349 926 901 701 671 262
197
SnaBI TAC'GTA
Cuts at: 493 6714 493
Size: 6221 2702
SpeI A'CTAG_T
Cuts at: 152 2716 6184 152
Size: 2564 3468 2891
Fragments arranged by size:
3468 2891 2564
SphI G_CATG'C
Cuts at: 1132 6281 1132
Size: 5149 3774
SspI AAT'ATT
Cuts at: 5 52 4945 6024 6847 5
Size: 47 4893 1079 823 2081
Fragments arranged by size:
4893 2081 1079 823 47
StuI AGG'CCT
Cuts at: 1517 2117 1517
Size: 600 8323
StyI C'CwwG_G
Cuts at: 513 1065 2004 2253 4482 5976 6362 513
Size: 552 939 249 2229 1494 386 3074
Fragments arranged by size:
3074 2229 1494 939 552 386 249
SwaI ATTT'AAAT
Cuts at: 4776 4776
Size: 8923
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2164 2168 6950 6967 7120 7305 7750
Size: 1255 584 4 4782 17 153 185 445
Cuts at: 7750 325
Size: 1498
Fragments arranged by size:
4782 1498 1255 584 445 185 153 17
4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2095 2548 2689
Size: 274 80 33 51 1443 118 453 141
Cuts at: 2689 3029 3097 3925 4034 4762 4908 4993 6096
Size: 340 68 828 109 728 146 85 1103
Cuts at: 6096 6154 7169 8881 96
Size: 58 1015 1712 138
Fragments arranged by size:
1712 1443 1103 1015 828 728 453 340
274 146 141 138 118 109 85 80
68 58 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8883 8915 8883
Size: 32 8891
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8923
XbaI T'CTAG_A
Cuts at: 2491 6259 2491
Size: 3768 5155
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8923
XmaI C'CCGG_G
Cuts at: 1870 6248 1870
Size: 4378 4545
XmnI GAAnn'nnTTC
Cuts at: 2624 3117 3279 7052 2624
Size: 493 162 3773 4495
Fragments arranged by size:
4495 3773 493 162
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6731 276
Size: 53 83 186 936 5197 2468
Fragments arranged by size:
5197 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NsiI NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BsiWI BstXI
BstZ17I DraIII FseI FspAI KasI NarI NruI PacI
PfoI PspXI RsrII SacII SanDI SexAI SfiI SfoI
SrfI XcmI