Restriction map for
pFN21AE5805
(Circular) (Six-base) MAPSORT of: pFN21AE5805.seq Check: 3384 from: 1 to: 7702
pFN21AE5805 7702 bp
With 182 enzymes: SgfI *
July 25, 2014 13:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2563 2709 2563
Size: 146 7556
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5512 278
Size: 53 83 186 936 3976 2468
Fragments arranged by size:
3976 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2289 5045 1937
Size: 352 2756 4594
Fragments arranged by size:
4594 2756 352
Acc65I G'GTAC_C
Cuts at: 5023 5023
Size: 7702
AclI AA'CG_TT
Cuts at: 1563 5829 6202 1563
Size: 4266 373 3063
Fragments arranged by size:
4266 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2031 2870 4592 4929 5765 7149 7417 1632
Size: 399 839 1722 337 836 1384 268 1917
Fragments arranged by size:
1917 1722 1384 839 836 399 337 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7505
AflIII A'CryG_T
Cuts at: 1203 6601 7578 1203
Size: 5398 977 1327
Fragments arranged by size:
5398 1327 977
AgeI A'CCGG_T
Cuts at: 6533 6533
Size: 7702
AhdI GACnn_n'nnGTC
Cuts at: 6431 6431
Size: 7702
AleI CACnn'nnGTG
Cuts at: 1166 4997 1166
Size: 3831 3871
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7702
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3596 3628 4604 4636 3596
Size: 32 976 32 6662
Fragments arranged by size:
6662 976 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2578 2689 5445 7017 2578
Size: 111 2756 1572 3263
Fragments arranged by size:
3263 2756 1572 111
ApaI G_GGCC'C
Cuts at: 2084 2128 2411 4660 2084
Size: 44 283 2249 5126
Fragments arranged by size:
5126 2249 283 44
ApaLI G'TGCA_C
Cuts at: 2327 2837 5759 6915 2327
Size: 510 2922 1156 3114
Fragments arranged by size:
3114 2922 1156 510
ApoI r'AATT_y
Cuts at: 1493 2474 2594 5011 5237 5389 7498 1493
Size: 981 120 2417 226 152 2109 1697
Fragments arranged by size:
2417 2109 1697 981 226 152 120
AseI AT'TA_AT
Cuts at: 160 1032 6256 160
Size: 872 5224 1606
Fragments arranged by size:
5224 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7702
AvaI C'yCGr_G
Cuts at: 1870 1943 2121 2709 2868 5027 1870
Size: 73 178 588 159 2159 4545
Fragments arranged by size:
4545 2159 588 178 159 73
BamHI G'GATC_C
Cuts at: 2540 5032 5399 2540
Size: 2492 367 4843
Fragments arranged by size:
4843 2492 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4288 5023 6478 618
Size: 325 217 627 2501 735 1455 1842
Fragments arranged by size:
2501 1842 1455 735 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2054 2084 2128 2411 2696 4023 4660 4898
Size: 663 30 44 283 285 1327 637 238
Cuts at: 4898 5021 1391
Size: 123 4072
Fragments arranged by size:
4072 1327 663 637 285 283 238 123
44 30
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3310 928
Size: 971 1411 5320
Fragments arranged by size:
5320 1411 971
BbvCI CC'TCA_GC
Cuts at: 3260 3260
Size: 7702
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5893 5927 1903
Size: 34 50 34 3872 34 3678
Fragments arranged by size:
3872 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2550 5594 6804 2550
Size: 3044 1210 3448
Fragments arranged by size:
3448 3044 1210
BclI T'GATC_A
Cuts at: 1548 4239 1548
Size: 2691 5011
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2588 4712 4962
Size: 107 122 71 631 605 916 2124 250
Cuts at: 4962 6313 136
Size: 1351 1525
Fragments arranged by size:
2124 1525 1351 916 631 605 250 122
107 71
BglII A'GATC_T
Cuts at: 3786 7697 3786
Size: 3911 3791
BlpI GC'TnA_GC
Cuts at: 2048 2690 3265 4034 4936 5119 2048
Size: 642 575 769 902 183 4631
Fragments arranged by size:
4631 902 769 642 575 183
Bme1580I G_kGCm'C
Cuts at: 1790 2084 2128 2331 2411 2841 3439 4660 5763
Size: 294 44 203 80 430 598 1221 1103
Cuts at: 5763 6919 1790
Size: 1156 2573
Fragments arranged by size:
2573 1221 1156 1103 598 430 294 203
80 44
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7702
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3398 5433 6391
Size: 416 53 83 399 24 1981 2035 958
Cuts at: 6391 442
Size: 1753
Fragments arranged by size:
2035 1981 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7702
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2465 2525 2746 3984 4161 6362 1239
Size: 1226 60 221 1238 177 2201 2579
Fragments arranged by size:
2579 2201 1238 1226 221 177 60
Bpu10I CC'TnA_GC
Cuts at: 3260 4751 3260
Size: 1491 6211
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2090 2531 3866 4850 5827 6692 6990 2090
Size: 441 1335 984 977 865 298 2802
Fragments arranged by size:
2802 1335 984 977 865 441 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4124 6365 882
Size: 617 2625 2241 2219
Fragments arranged by size:
2625 2241 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5493 493
Size: 711 4289 2702
Fragments arranged by size:
4289 2702 711
BsaBI GATnn'nnATC
Cuts at: 7696 7696
Size: 7702
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5509 5891 6526 275
Size: 53 83 186 936 3976 382 635 1451
Fragments arranged by size:
3976 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2313 2553 4222 6135 6533 6807 6954 7365 2313
Size: 240 1669 1913 398 274 147 411 2650
Fragments arranged by size:
2650 1913 1669 411 398 274 240 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2010 2040 2744 2759 2774
Size: 30 165 30 607 30 704 15 15
Cuts at: 2774 2789 3126 3156 3596 3626 4484 4514 4532
Size: 15 337 30 440 30 858 30 18
Cuts at: 4532 4562 4604 4634 1178
Size: 30 42 30 4246
Fragments arranged by size:
4246 858 704 607 440 337 165 42
30 30 30 30 30 30 30 30
18 15 15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2462 2465 4295 4865 4906 1186
Size: 1276 3 1830 570 41 3982
Fragments arranged by size:
3982 1830 1276 570 41 3
BseYI C'CCAG_C
Cuts at: 2402 2425 3091 3182 4960 6905 7401 2402
Size: 23 666 91 1778 1945 496 2703
Fragments arranged by size:
2703 1945 1778 666 496 91 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4508 1163
Size: 573 2772 4357
Fragments arranged by size:
4357 2772 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4367 5152 5913 6941 1540
Size: 275 186 2366 785 761 1028 2301
Fragments arranged by size:
2366 2301 1028 785 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2186 2331 2484 2696 2841 4000 4023 5763 5848
Size: 145 153 212 145 1159 23 1740 85
Cuts at: 5848 6919 2186
Size: 1071 2969
Fragments arranged by size:
2969 1740 1159 1071 212 153 145 145
85 23
BsmI GAATG_Cn'
Cuts at: 3214 4026 5213 5306 3214
Size: 812 1187 93 5610
Fragments arranged by size:
5610 1187 812 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2374 4354 2374
Size: 1980 5722
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2054 2084 2128 2186 2331 2411 2484
Size: 399 264 30 44 58 145 80 73
Cuts at: 2484 2696 2841 3439 4000 4023 4660 4898 5021
Size: 212 145 598 561 23 637 238 123
Cuts at: 5021 5763 5848 6919 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 637 598 561 399 264
238 212 145 145 123 85 80 73
58 44 30 23
BspEI T'CCGG_A
Cuts at: 2553 4222 7365 2553
Size: 1669 3143 2890
Fragments arranged by size:
3143 2890 1669
BspHI T'CATG_A
Cuts at: 2938 3624 5591 7321 2938
Size: 686 1967 1730 3319
Fragments arranged by size:
3319 1967 1730 686
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2563 2709 4617 5057 844
Size: 1719 146 1908 440 3489
Fragments arranged by size:
3489 1908 1719 440 146
BsrBI CCG'CTC
Cuts at: 1394 1823 2434 3689 5589 1394
Size: 429 611 1255 1900 3507
Fragments arranged by size:
3507 1900 1255 611 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2655 4455 6197 6371 65
Size: 1183 1407 1800 1742 174 1396
Fragments arranged by size:
1800 1742 1407 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2043 4882 6346 6533 1700
Size: 110 233 2839 1464 187 2869
Fragments arranged by size:
2869 2839 1464 233 187 110
BsrGI T'GTAC_A
Cuts at: 96 2781 96
Size: 2685 5017
BssHII G'CGCG_C
Cuts at: 1925 3005 1925
Size: 1080 6622
BssSI C'ACGA_G
Cuts at: 2840 3761 4739 4760 5762 6774 2840
Size: 921 978 21 1002 1012 3768
Fragments arranged by size:
3768 1012 1002 978 921 21
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2862 794
Size: 2068 5634
BstBI TT'CG_AA
Cuts at: 4114 4163 4679 6506 4114
Size: 49 516 1827 5310
Fragments arranged by size:
5310 1827 516 49
BstEII G'GTnAC_C
Cuts at: 1184 3593 3977 1184
Size: 2409 384 4909
Fragments arranged by size:
4909 2409 384
BstXI CCAn_nnnn'nTGG
Cuts at: 3358 4179 3358
Size: 821 6881
BstYI r'GATC_y
Cuts at: 1972 2155 2540 2844 3786 5032 5399 5784 5801
Size: 183 385 304 942 1246 367 385 17
Cuts at: 5801 7253 7339 7351 7697 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1246 942 385 385 367 346
304 183 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2055 2055
Size: 7702
BtgI C'CryG_G
Cuts at: 513 1065 2567 4652 4788 513
Size: 552 1502 2085 136 3427
Fragments arranged by size:
3427 2085 1502 552 136
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3561 487
Size: 683 56 2335 4628
Fragments arranged by size:
4628 2335 683 56
BtsI GCAGTG_nn'
Cuts at: 2105 4184 5218 6012 6032 2105
Size: 2079 1034 794 20 3775
Fragments arranged by size:
3775 2079 1034 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7702
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7667
DraI TTT'AAA
Cuts at: 5006 5006
Size: 7702
DraIII CAC_nnn'GTG
Cuts at: 3222 3222
Size: 7702
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2100 6709 809
Size: 507 784 4609 1802
Fragments arranged by size:
4609 1802 784 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2161 2350 2580
Size: 54 1425 50 275 18 331 189 230
Cuts at: 2580 3801 4364 5149 6038 7667 8
Size: 1221 563 785 889 1629 43
Fragments arranged by size:
1629 1425 1221 889 785 563 331 275
230 189 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4364 5149 1537
Size: 275 2552 785 4090
Fragments arranged by size:
4090 2552 785 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2940 3365 3414 3846 3984 4271 4814 5632
Size: 1587 425 49 432 138 287 543 818
Cuts at: 5632 1353
Size: 3423
Fragments arranged by size:
3423 1587 818 543 432 425 287 138
49
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6289 6663 6809 387
Size: 1064 138 4700 374 146 1280
Fragments arranged by size:
4700 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2694 4021 2694
Size: 1327 6375
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2031 2465 2525 2746 2870 3984 4161
Size: 393 399 434 60 221 124 1114 177
Cuts at: 4161 4592 4929 5765 6362 7149 7417 1239
Size: 431 337 836 597 787 268 1524
Fragments arranged by size:
1524 1114 836 787 597 434 431 399
393 337 268 221 177 124 60
EcoNI CCTnn'n_nnAGG
Cuts at: 2058 2447 4847 2058
Size: 389 2400 4913
Fragments arranged by size:
4913 2400 389
EcoO109I rG'GnC_Cy
Cuts at: 2080 2081 2117 2125 2407 3403 4183 4657 2080
Size: 1 36 8 282 996 780 474 5125
Fragments arranged by size:
5125 996 780 474 282 36 8 1
EcoRI G'AATT_C
Cuts at: 2594 5011 5389 2594
Size: 2417 378 4907
Fragments arranged by size:
4907 2417 378
EcoRV GAT'ATC
Cuts at: 4390 4390
Size: 7702
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2821 2821
Size: 7702
FspI TGC'GCA
Cuts at: 6208 6208
Size: 7702
HaeII r_GCGC'y
Cuts at: 1678 3798 3859 6849 1678
Size: 2120 61 2990 2531
Fragments arranged by size:
2990 2531 2120 61
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2101 2133 2219 2251 3652
Size: 32 135 32 529 32 86 32 1401
Cuts at: 3652 3684 4397 4429 6343 6375 6417 6449 1373
Size: 32 713 32 1914 32 42 32 2626
Fragments arranged by size:
2626 1914 1401 713 529 135 86 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2290 4537 5046 5292 669
Size: 988 281 352 2247 509 246 3079
Fragments arranged by size:
3079 2247 988 509 352 281 246
HindIII A'AGCT_T
Cuts at: 748 3870 748
Size: 3122 4580
HpaI GTT'AAC
Cuts at: 5292 5292
Size: 7702
KpnI G_GTAC'C
Cuts at: 5027 5027
Size: 7702
MfeI C'AATT_G
Cuts at: 3165 5301 3165
Size: 2136 5566
MluI A'CGCG_T
Cuts at: 7578 7578
Size: 7702
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2172 3055 4494 6816 7000 1305
Size: 867 883 1439 2322 184 2007
Fragments arranged by size:
2322 2007 1439 883 867 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2163 2352 2582 3803 10
Size: 54 1425 343 331 189 230 1221 3909
Fragments arranged by size:
3909 1425 1221 343 331 230 189 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2268 2943 4043 4235 4322 4995 4997
Size: 648 1102 675 1100 192 87 673 2
Cuts at: 4997 5660 6019 6178 518
Size: 663 359 159 2042
Fragments arranged by size:
2042 1102 1100 675 673 663 648 359
192 159 87 2
MspA1I CmG'CkG
Cuts at: 1606 2467 4570 4960 5118 5795 6943 7188 1606
Size: 861 2103 390 158 677 1148 245 2120
Fragments arranged by size:
2120 2103 1148 861 677 390 245 158
NaeI GCC'GGC
Cuts at: 2045 4884 2045
Size: 2839 4863
NcoI C'CATG_G
Cuts at: 513 1065 4652 513
Size: 552 3587 3563
Fragments arranged by size:
3587 3563 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7702
NgoMIV G'CCGG_C
Cuts at: 2043 4882 2043
Size: 2839 4863
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7702
NotI GC'GGCC_GC
Cuts at: 5149 5149
Size: 7702
NspI r_CATG'y
Cuts at: 1132 1229 2263 5060 6605 1132
Size: 97 1034 2797 1545 2229
Fragments arranged by size:
2797 2229 1545 1034 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7702
PciI A'CATG_T
Cuts at: 6601 6601
Size: 7702
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2359 3520 4904 1108
Size: 1251 1161 1384 3906
Fragments arranged by size:
3906 1384 1251 1161
PfoI T'CCnGG_A
Cuts at: 2790 3357 3777 2790
Size: 567 420 6715
Fragments arranged by size:
6715 567 420
PmeI GTTT'AAAC
Cuts at: 5006 5006
Size: 7702
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3248 3280 3596 3628 4604 4636 5771 5803 7284
Size: 32 316 32 976 32 1135 32 1481
Cuts at: 7284 7316 3248
Size: 32 3634
Fragments arranged by size:
3634 1481 1135 976 316 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 2117 3403 4183 2117
Size: 1286 780 5636
Fragments arranged by size:
5636 1286 780
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7702
PsiI TTA'TAA
Cuts at: 5272 5272
Size: 7702
PspOMI G'GGCC_C
Cuts at: 2080 2124 2407 4656 2080
Size: 44 283 2249 5126
Fragments arranged by size:
5126 2249 283 44
PstI C_TGCA'G
Cuts at: 830 2139 2685 2706 3685 4628 5054 830
Size: 1309 546 21 979 943 426 3478
Fragments arranged by size:
3478 1309 979 943 546 426 21
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7702
PvuII CAG'CTG
Cuts at: 4570 4960 4570
Size: 390 7312
SacI G_AGCT'C
Cuts at: 2696 4023 2696
Size: 1327 6375
SalI G'TCGA_C
Cuts at: 1936 2288 5044 1936
Size: 352 2756 4594
Fragments arranged by size:
4594 2756 352
SapI GCTCTTCn'nnn_
Cuts at: 3846 4814 3846
Size: 968 6734
SbfI CC_TGCA'GG
Cuts at: 5054 5054
Size: 7702
ScaI AGT'ACT
Cuts at: 4499 4688 5950 7662 4499
Size: 189 1262 1712 4539
Fragments arranged by size:
4539 1712 1262 189
SfcI C'TryA_G
Cuts at: 826 1045 2135 2681 2702 3377 3681 4192 4504
Size: 219 1090 546 21 675 304 511 312
Cuts at: 4504 4624 5050 6185 6866 7057 826
Size: 120 426 1135 681 191 1471
Fragments arranged by size:
1471 1135 1090 681 675 546 511 426
312 304 219 191 120 21
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7702
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7702
SmaI CCC'GGG
Cuts at: 1872 2123 5029 1872
Size: 251 2906 4545
Fragments arranged by size:
4545 2906 251
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2105 2510 3881 4865 5806 6707
Size: 197 926 162 405 1371 984 941 901
Cuts at: 6707 6969 820
Size: 262 1553
Fragments arranged by size:
1553 1371 984 941 926 901 405 262
197 162
SnaBI TAC'GTA
Cuts at: 493 5493 493
Size: 5000 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7702
SphI G_CATG'C
Cuts at: 1132 2263 5060 1132
Size: 1131 2797 3774
Fragments arranged by size:
3774 2797 1131
SspI AAT'ATT
Cuts at: 5 52 5626 5
Size: 47 5574 2081
Fragments arranged by size:
5574 2081 47
StuI AGG'CCT
Cuts at: 1517 3454 3949 1517
Size: 1937 495 5270
Fragments arranged by size:
5270 1937 495
StyI C'CwwG_G
Cuts at: 513 1065 2238 2338 4652 5141 513
Size: 552 1173 100 2314 489 3074
Fragments arranged by size:
3074 2314 1173 552 489 100
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4148 5729 5746 5899 6084 6529 325
Size: 1255 2568 1581 17 153 185 445 1498
Fragments arranged by size:
2568 1581 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2296 2781 4227
Size: 274 80 33 51 1443 319 485 1446
Cuts at: 4227 4497 4686 5948 7660 96
Size: 270 189 1262 1712 138
Fragments arranged by size:
1712 1446 1443 1262 485 319 274 270
189 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3126 3158 3208 3240 4532 4564 7662 7694 3126
Size: 32 50 32 1292 32 3098 32 3134
Fragments arranged by size:
3134 3098 1292 50 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7702
XbaI T'CTAG_A
Cuts at: 5038 5038
Size: 7702
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2171 2345 2881 3913 2171
Size: 174 536 1032 5960
Fragments arranged by size:
5960 1032 536 174
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7702
XmaI C'CCGG_G
Cuts at: 1870 2121 5027 1870
Size: 251 2906 4545
Fragments arranged by size:
4545 2906 251
XmnI GAAnn'nnTTC
Cuts at: 3177 3212 5831 3177
Size: 35 2619 5048
Fragments arranged by size:
5048 2619 35
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5510 276
Size: 53 83 186 936 3976 2468
Fragments arranged by size:
3976 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BstZ17I FseI FspAI KasI NarI NruI NsiI PacI
PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI
SfoI SrfI SwaI