Restriction map for
pFN21AE5794
(Circular) (Six-base) MAPSORT of: pFN21AE5794.seq Check: 1417 from: 1 to: 6772
pFN21AE5794 6772 bp
With 182 enzymes: SgfI *
December 8, 2014 13:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2738 2738
Size: 6772
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4582 278
Size: 53 83 186 936 3046 2468
Fragments arranged by size:
3046 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2723 4115 1937
Size: 786 1392 4594
Fragments arranged by size:
4594 1392 786
Acc65I G'GTAC_C
Cuts at: 4093 4093
Size: 6772
AclI AA'CG_TT
Cuts at: 1563 2330 4899 5272 1563
Size: 767 2569 373 3063
Fragments arranged by size:
3063 2569 767 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2571 3470 3533 4835 6219 6487 1632
Size: 939 899 63 1302 1384 268 1917
Fragments arranged by size:
1917 1384 1302 939 899 268 63
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6575
AflIII A'CryG_T
Cuts at: 1203 5671 6648 1203
Size: 4468 977 1327
Fragments arranged by size:
4468 1327 977
AgeI A'CCGG_T
Cuts at: 2219 5603 2219
Size: 3384 3388
AhdI GACnn_n'nnGTC
Cuts at: 5501 5501
Size: 6772
AleI CACnn'nnGTG
Cuts at: 1166 3533 1166
Size: 2367 4405
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3281 3635 1184
Size: 2097 354 4321
Fragments arranged by size:
4321 2097 354
AlwNI CAG_nnn'CTG
Cuts at: 2662 2804 2899 4515 6087 2662
Size: 142 95 1616 1572 3347
Fragments arranged by size:
3347 1616 1572 142 95
ApaLI G'TGCA_C
Cuts at: 4829 5985 4829
Size: 1156 5616
ApoI r'AATT_y
Cuts at: 1493 2836 4081 4307 4459 6568 1493
Size: 1343 1245 226 152 2109 1697
Fragments arranged by size:
2109 1697 1343 1245 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5326 160
Size: 872 4294 1606
Fragments arranged by size:
4294 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6772
AvaI C'yCGr_G
Cuts at: 1870 1943 2142 3626 3816 3955 4097 1870
Size: 73 199 1484 190 139 142 4545
Fragments arranged by size:
4545 1484 199 190 142 139 73
BamHI G'GATC_C
Cuts at: 3845 4102 4469 3845
Size: 257 367 6148
Fragments arranged by size:
6148 367 257
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2750 2902 3265 3419 4093
Size: 325 217 627 963 152 363 154 674
Cuts at: 4093 5548 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 963 674 627 363 325 217
154 152
BanII G_rGCy'C
Cuts at: 1391 3305 3899 4091 1391
Size: 1914 594 192 4072
Fragments arranged by size:
4072 1914 594 192
BbeI G_GCGC'C
Cuts at: 3423 3423
Size: 6772
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2337 928
Size: 971 438 5363
Fragments arranged by size:
5363 971 438
BbvCI CC'TCA_GC
Cuts at: 2208 2208
Size: 6772
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4963 4997 1903
Size: 34 3026 34 3678
Fragments arranged by size:
3678 3026 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4664 5874 4664
Size: 1210 5562
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6772
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5383 136
Size: 107 122 71 631 605 3711 1525
Fragments arranged by size:
3711 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6767 6767
Size: 6772
BlpI GC'TnA_GC
Cuts at: 2394 4189 2394
Size: 1795 4977
Bme1580I G_kGCm'C
Cuts at: 1790 3270 3624 4833 5989 1790
Size: 1480 354 1209 1156 2573
Fragments arranged by size:
2573 1480 1209 1156 354
BmgBI CAC'GTC
Cuts at: 1318 3334 1318
Size: 2016 4756
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2753 4503 5461
Size: 416 53 83 399 24 1336 1750 958
Cuts at: 5461 442
Size: 1753
Fragments arranged by size:
1753 1750 1336 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6772
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2104 2658 2727 3452 5432 1239
Size: 865 554 69 725 1980 2579
Fragments arranged by size:
2579 1980 865 725 554 69
Bpu10I CC'TnA_GC
Cuts at: 2208 3936 2208
Size: 1728 5044
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3029 4897 5762 6060 3029
Size: 1868 865 298 3741
Fragments arranged by size:
3741 1868 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2049 2433 2815 5435 882
Size: 617 550 384 382 2620 2219
Fragments arranged by size:
2620 2219 617 550 384 382
BsaAI yAC'GTr
Cuts at: 493 1204 4563 493
Size: 711 3359 2702
Fragments arranged by size:
3359 2702 711
BsaBI GATnn'nnATC
Cuts at: 6766 6766
Size: 6772
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3420 4579 4961 5596
Size: 53 83 186 936 1887 1159 382 635
Cuts at: 5596 275
Size: 1451
Fragments arranged by size:
1887 1451 1159 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2219 3929 5205 5603 5877 6024 6435 2219
Size: 1710 1276 398 274 147 411 2556
Fragments arranged by size:
2556 1710 1276 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2043 2073 2102 2132 2169
Size: 30 165 30 640 30 29 30 37
Cuts at: 2169 2199 2768 2798 1178
Size: 30 569 30 5152
Fragments arranged by size:
5152 640 569 165 37 30 30 30
30 30 30 29
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2553 3379 4001 1186
Size: 1367 826 622 3957
Fragments arranged by size:
3957 1367 826 622
BseYI C'CCAG_C
Cuts at: 2159 2717 3083 3805 3918 5975 6471 2159
Size: 558 366 722 113 2057 496 2460
Fragments arranged by size:
2460 2057 722 558 496 366 113
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3356 1163
Size: 573 1620 4579
Fragments arranged by size:
4579 1620 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3534 4222 4983 6011 1540
Size: 275 186 1533 688 761 1028 2301
Fragments arranged by size:
2301 1533 1028 761 688 275 186
BsiHKAI G_wGCw'C
Cuts at: 3633 3793 3937 4833 4918 5989 3633
Size: 160 144 896 85 1071 4416
Fragments arranged by size:
4416 1071 896 160 144 85
BsmI GAATG_Cn'
Cuts at: 2039 4283 4376 2039
Size: 2244 93 4435
Fragments arranged by size:
4435 2244 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3270 3305 3624 3633 3793 3899 3937
Size: 399 1480 35 319 9 160 106 38
Cuts at: 3937 4091 4833 4918 5989 1391
Size: 154 742 85 1071 2174
Fragments arranged by size:
2174 1480 1071 742 399 319 160 154
106 85 38 35 9
BspEI T'CCGG_A
Cuts at: 6435 6435
Size: 6772
BspHI T'CATG_A
Cuts at: 2017 3411 4661 6391 2017
Size: 1394 1250 1730 2398
Fragments arranged by size:
2398 1730 1394 1250
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2722 2738 4127 844
Size: 1878 16 1389 3489
Fragments arranged by size:
3489 1878 1389 16
BsrBI CCG'CTC
Cuts at: 1394 1823 3504 4659 1394
Size: 429 1681 1155 3507
Fragments arranged by size:
3507 1681 1155 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3246 5267 5441 65
Size: 1183 1998 2021 174 1396
Fragments arranged by size:
2021 1998 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2219 3883 5416 5603 1700
Size: 110 409 1664 1533 187 2869
Fragments arranged by size:
2869 1664 1533 409 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6772
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6772
BssSI C'ACGA_G
Cuts at: 2826 4832 5844 2826
Size: 2006 1012 3754
Fragments arranged by size:
3754 2006 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2804 3998 794
Size: 2010 1194 3568
Fragments arranged by size:
3568 2010 1194
BstBI TT'CG_AA
Cuts at: 5576 5576
Size: 6772
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6772
BstXI CCAn_nnnn'nTGG
Cuts at: 3241 3560 3241
Size: 319 6453
BstYI r'GATC_y
Cuts at: 1972 3309 3845 4102 4469 4854 4871 6323 6409
Size: 1337 536 257 367 385 17 1452 86
Cuts at: 6409 6421 6767 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1337 536 385 367 346 257
86 17 12
BstZ17I GTA'TAC
Cuts at: 2724 2724
Size: 6772
Bsu36I CC'TnA_GG
Cuts at: 2772 2772
Size: 6772
BtgI C'CryG_G
Cuts at: 513 1065 2957 3181 513
Size: 552 1892 224 4104
Fragments arranged by size:
4104 1892 552 224
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6033
Fragments arranged by size:
6033 683 56
BtsI GCAGTG_nn'
Cuts at: 3496 4288 5082 5102 3496
Size: 792 794 20 5166
Fragments arranged by size:
5166 794 792 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6772
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6737
DraI TTT'AAA
Cuts at: 4076 4076
Size: 6772
DraIII CAC_nnn'GTG
Cuts at: 2527 2527
Size: 6772
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3011 5779 809
Size: 507 1695 2768 1802
Fragments arranged by size:
2768 1802 1695 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3649 3947 4219
Size: 54 1425 50 275 18 1819 298 272
Cuts at: 4219 5108 6737 8
Size: 889 1629 43
Fragments arranged by size:
1819 1629 1425 889 298 275 272 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4219 1537
Size: 275 2407 4090
Fragments arranged by size:
4090 2407 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3698 3869 4702 1353
Size: 2345 171 833 3423
Fragments arranged by size:
3423 2345 833 171
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3694 5359 5733 5879 387
Size: 1064 138 2105 1665 374 146 1280
Fragments arranged by size:
2105 1665 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2104 2571 2658 2727 3452 3470 3533
Size: 393 472 467 87 69 725 18 63
Cuts at: 3533 4835 5432 6219 6487 1239
Size: 1302 597 787 268 1524
Fragments arranged by size:
1524 1302 787 725 597 472 467 393
268 87 69 63 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2745 2745
Size: 6772
EcoO109I rG'GnC_Cy
Cuts at: 2991 3463 3809 2991
Size: 472 346 5954
Fragments arranged by size:
5954 472 346
EcoRI G'AATT_C
Cuts at: 4081 4459 4081
Size: 378 6394
FspI TGC'GCA
Cuts at: 3488 5278 3488
Size: 1790 4982
HaeII r_GCGC'y
Cuts at: 1678 3033 3423 5919 1678
Size: 1355 390 2496 2531
Fragments arranged by size:
2531 2496 1355 390
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2169 2201 2768 2800 5413
Size: 32 135 32 597 32 567 32 2613
Cuts at: 5413 5445 5487 5519 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2613 597 567 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4116 4362 669
Size: 988 281 2178 246 3079
Fragments arranged by size:
3079 2178 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6772
HpaI GTT'AAC
Cuts at: 4362 4362
Size: 6772
KasI G'GCGC_C
Cuts at: 3419 3419
Size: 6772
KpnI G_GTAC'C
Cuts at: 4097 4097
Size: 6772
MfeI C'AATT_G
Cuts at: 2633 4371 2633
Size: 1738 5034
MluI A'CGCG_T
Cuts at: 6648 6648
Size: 6772
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2660 5886 6070 1305
Size: 1355 3226 184 2007
Fragments arranged by size:
3226 2007 1355 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3651 3949 10
Size: 54 1425 343 1819 298 2833
Fragments arranged by size:
2833 1819 1425 343 298 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3123 3533 3617 4730 5089 5248 518
Size: 648 1957 410 84 1113 359 159 2042
Fragments arranged by size:
2042 1957 1113 648 410 359 159 84
MspA1I CmG'CkG
Cuts at: 1606 2097 2662 2801 3019 3433 3786 3805 4188
Size: 491 565 139 218 414 353 19 383
Cuts at: 4188 4865 6013 6258 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 565 491 414 383 353
245 218 139 19
NaeI GCC'GGC
Cuts at: 3885 3885
Size: 6772
NarI GG'CG_CC
Cuts at: 3420 3420
Size: 6772
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6220
NdeI CA'TA_TG
Cuts at: 387 2533 387
Size: 2146 4626
NgoMIV G'CCGG_C
Cuts at: 3883 3883
Size: 6772
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6772
NotI GC'GGCC_GC
Cuts at: 4219 4219
Size: 6772
NspI r_CATG'y
Cuts at: 1132 1229 2248 3979 4130 5675 1132
Size: 97 1019 1731 151 1545 2229
Fragments arranged by size:
2229 1731 1545 1019 151 97
PasI CC'CwG_GG
Cuts at: 1348 3674 1348
Size: 2326 4446
PciI A'CATG_T
Cuts at: 5671 5671
Size: 6772
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2899 3022 1108
Size: 1791 123 4858
Fragments arranged by size:
4858 1791 123
PmeI GTTT'AAAC
Cuts at: 4076 4076
Size: 6772
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3752 3784 4841 4873 6354 6386 3752
Size: 32 1057 32 1481 32 4138
Fragments arranged by size:
4138 1481 1057 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2991 3463 3809 2991
Size: 472 346 5954
Fragments arranged by size:
5954 472 346
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6772
PsiI TTA'TAA
Cuts at: 4342 4342
Size: 6772
PstI C_TGCA'G
Cuts at: 830 2418 3157 4124 830
Size: 1588 739 967 3478
Fragments arranged by size:
3478 1588 967 739
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6772
PvuII CAG'CTG
Cuts at: 2097 2801 3433 3786 3805 2097
Size: 704 632 353 19 5064
Fragments arranged by size:
5064 704 632 353 19
SalI G'TCGA_C
Cuts at: 1936 4114 1936
Size: 2178 4594
SanDI GG'GwC_CC
Cuts at: 2991 3809 2991
Size: 818 5954
SapI GCTCTTCn'nnn_
Cuts at: 3869 3869
Size: 6772
SbfI CC_TGCA'GG
Cuts at: 4124 4124
Size: 6772
ScaI AGT'ACT
Cuts at: 2653 5020 6732 2653
Size: 2367 1712 2693
Fragments arranged by size:
2693 2367 1712
SexAI A'CCwGG_T
Cuts at: 3261 3261
Size: 6772
SfcI C'TryA_G
Cuts at: 826 1045 2414 3153 3716 4120 5255 5936 6127
Size: 219 1369 739 563 404 1135 681 191
Cuts at: 6127 826
Size: 1471
Fragments arranged by size:
1471 1369 1135 739 681 563 404 219
191
SfoI GGC'GCC
Cuts at: 3421 3421
Size: 6772
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6772
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6772
SmaI CCC'GGG
Cuts at: 1872 4099 1872
Size: 2227 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3008 4876 5777 6039 820
Size: 197 926 1065 1868 901 262 1553
Fragments arranged by size:
1868 1553 1065 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4563 493
Size: 4070 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6772
SphI G_CATG'C
Cuts at: 1132 2248 3979 4130 1132
Size: 1116 1731 151 3774
Fragments arranged by size:
3774 1731 1116 151
SspI AAT'ATT
Cuts at: 5 52 4696 5
Size: 47 4644 2081
Fragments arranged by size:
4644 2081 47
StuI AGG'CCT
Cuts at: 1517 3288 3826 3941 1517
Size: 1771 538 115 4348
Fragments arranged by size:
4348 1771 538 115
StyI C'CwwG_G
Cuts at: 513 1065 4211 513
Size: 552 3146 3074
Fragments arranged by size:
3146 3074 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4799 4816 4969 5154 5599 325
Size: 1255 3219 17 153 185 445 1498
Fragments arranged by size:
3219 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2651 5018 6730
Size: 274 80 33 51 1443 674 2367 1712
Cuts at: 6730 96
Size: 138
Fragments arranged by size:
2367 1712 1443 674 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2102 2134 2448 2480 3386 3418 6732 6764 2102
Size: 32 314 32 906 32 3314 32 2110
Fragments arranged by size:
3314 2110 906 314 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6772
XbaI T'CTAG_A
Cuts at: 4108 4108
Size: 6772
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2077 2188 3023 3259 3430 2077
Size: 111 835 236 171 5419
Fragments arranged by size:
5419 835 236 171 111
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6772
XmaI C'CCGG_G
Cuts at: 1870 4097 1870
Size: 2227 4545
XmnI GAAnn'nnTTC
Cuts at: 2037 4901 2037
Size: 2864 3908
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4580 276
Size: 53 83 186 936 3046 2468
Fragments arranged by size:
3046 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI EcoICRI EcoRV FalI FseI FspAI NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII
SacI SacII SfiI SrfI SwaI