Restriction map for
pFN21AE5791
(Circular) (Six-base) MAPSORT of: pFN21AE5791.seq Check: 9501 from: 1 to: 6598
pFN21AE5791 6598 bp
With 182 enzymes: SgfI *
December 8, 2014 13:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2569 2569
Size: 6598
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4408 278
Size: 53 83 186 936 2872 2468
Fragments arranged by size:
2872 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3941 1937
Size: 2004 4594
Acc65I G'GTAC_C
Cuts at: 3919 3919
Size: 6598
AclI AA'CG_TT
Cuts at: 1563 4725 5098 1563
Size: 3162 373 3063
Fragments arranged by size:
3162 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2292 4661 6045 6313 1632
Size: 660 2369 1384 268 1917
Fragments arranged by size:
2369 1917 1384 660 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6401
AflIII A'CryG_T
Cuts at: 1203 2615 3393 3616 5497 6474 1203
Size: 1412 778 223 1881 977 1327
Fragments arranged by size:
1881 1412 1327 977 778 223
AgeI A'CCGG_T
Cuts at: 5429 5429
Size: 6598
AhdI GACnn_n'nnGTC
Cuts at: 5327 5327
Size: 6598
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6598
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2578 1184
Size: 1394 5204
AlwNI CAG_nnn'CTG
Cuts at: 2097 3242 4341 5913 2097
Size: 1145 1099 1572 2782
Fragments arranged by size:
2782 1572 1145 1099
ApaI G_GGCC'C
Cuts at: 2020 2020
Size: 6598
ApaLI G'TGCA_C
Cuts at: 4655 5811 4655
Size: 1156 5442
ApoI r'AATT_y
Cuts at: 1493 2286 2602 3158 3380 3756 3907 4133 4285
Size: 793 316 556 222 376 151 226 152
Cuts at: 4285 6394 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 793 556 376 316 226 222
152 151
AseI AT'TA_AT
Cuts at: 160 1032 5152 160
Size: 872 4120 1606
Fragments arranged by size:
4120 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6598
AvaI C'yCGr_G
Cuts at: 1870 1943 3588 3923 1870
Size: 73 1645 335 4545
Fragments arranged by size:
4545 1645 335 73
BamHI G'GATC_C
Cuts at: 3928 4295 3928
Size: 367 6231
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3919 5374 618
Size: 325 217 627 2132 1455 1842
Fragments arranged by size:
2132 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2020 3370 3917 1391
Size: 629 1350 547 4072
Fragments arranged by size:
4072 1350 629 547
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3030 928
Size: 971 1131 4496
Fragments arranged by size:
4496 1131 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4789 4823 1903
Size: 34 2852 34 3678
Fragments arranged by size:
3678 2852 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3595 4490 5700 3595
Size: 895 1210 4493
Fragments arranged by size:
4493 1210 895
BclI T'GATC_A
Cuts at: 1548 3517 1548
Size: 1969 4629
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2112 2324 5209
Size: 107 122 71 631 605 440 212 2885
Cuts at: 5209 136
Size: 1525
Fragments arranged by size:
2885 1525 631 605 440 212 122 107
71
BglII A'GATC_T
Cuts at: 2221 2512 2785 3790 6593 2221
Size: 291 273 1005 2803 2226
Fragments arranged by size:
2803 2226 1005 291 273
BlpI GC'TnA_GC
Cuts at: 2445 3807 4015 2445
Size: 1362 208 5028
Fragments arranged by size:
5028 1362 208
Bme1580I G_kGCm'C
Cuts at: 1790 2020 4659 5815 1790
Size: 230 2639 1156 2573
Fragments arranged by size:
2639 2573 1156 230
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6598
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2023 4329 5287
Size: 416 53 83 399 24 606 2306 958
Cuts at: 5287 442
Size: 1753
Fragments arranged by size:
2306 1753 958 606 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6598
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2119 2611 5258 1239
Size: 880 492 2647 2579
Fragments arranged by size:
2647 2579 880 492
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4723 5588 5886 4723
Size: 865 298 5435
Fragments arranged by size:
5435 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2046 2978 5261 882
Size: 617 547 932 2283 2219
Fragments arranged by size:
2283 2219 932 617 547
BsaAI yAC'GTr
Cuts at: 493 1204 2938 4389 493
Size: 711 1734 1451 2702
Fragments arranged by size:
2702 1734 1451 711
BsaBI GATnn'nnATC
Cuts at: 6592 6592
Size: 6598
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4405 4787 5422 275
Size: 53 83 186 936 2872 382 635 1451
Fragments arranged by size:
2872 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2807 3784 5031 5429 5703 5850 6261 2807
Size: 977 1247 398 274 147 411 3144
Fragments arranged by size:
3144 1247 977 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6373
Fragments arranged by size:
6373 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6598
BseYI C'CCAG_C
Cuts at: 2067 5801 6297 2067
Size: 3734 496 2368
Fragments arranged by size:
3734 2368 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6025
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4048 4809 5837 1540
Size: 275 186 2047 761 1028 2301
Fragments arranged by size:
2301 2047 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3370 4659 4744 5815 3370
Size: 1289 85 1071 4153
Fragments arranged by size:
4153 1289 1071 85
BsmI GAATG_Cn'
Cuts at: 4109 4202 4109
Size: 93 6505
BsmBI CGTCTCn'nnnn_
Cuts at: 2651 2651
Size: 6598
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2020 3370 3917 4659 4744 5815 1391
Size: 399 230 1350 547 742 85 1071 2174
Fragments arranged by size:
2174 1350 1071 742 547 399 230 85
BspEI T'CCGG_A
Cuts at: 3784 6261 3784
Size: 2477 4121
BspHI T'CATG_A
Cuts at: 4487 6217 4487
Size: 1730 4868
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2569 2658 3953 844
Size: 1725 89 1295 3489
Fragments arranged by size:
3489 1725 1295 89
BsrBI CCG'CTC
Cuts at: 1394 1823 4485 1394
Size: 429 2662 3507
Fragments arranged by size:
3507 2662 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5093 5267 65
Size: 1183 3845 174 1396
Fragments arranged by size:
3845 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3740 5242 5429 1700
Size: 110 1930 1502 187 2869
Fragments arranged by size:
2869 1930 1502 187 110
BsrGI T'GTAC_A
Cuts at: 96 3658 96
Size: 3562 3036
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6598
BssSI C'ACGA_G
Cuts at: 4658 5670 4658
Size: 1012 5586
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6598
BstBI TT'CG_AA
Cuts at: 3731 5402 3731
Size: 1671 4927
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6598
BstYI r'GATC_y
Cuts at: 1972 2221 2512 2785 3274 3790 3928 4295 4680
Size: 249 291 273 489 516 138 367 385
Cuts at: 4680 4697 6149 6235 6247 6593 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 516 489 385 367 346 291
273 249 138 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6046
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5859
Fragments arranged by size:
5859 683 56
BtsI GCAGTG_nn'
Cuts at: 2098 4114 4908 4928 2098
Size: 2016 794 20 3768
Fragments arranged by size:
3768 2016 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6598
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6563
DraI TTT'AAA
Cuts at: 2164 2674 3544 3902 2164
Size: 510 870 358 4860
Fragments arranged by size:
4860 870 510 358
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5605 809
Size: 507 4289 1802
Fragments arranged by size:
4289 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2113 2762 3233
Size: 54 1425 50 275 18 283 649 471
Cuts at: 3233 3738 4045 4934 6563 8
Size: 505 307 889 1629 43
Fragments arranged by size:
1629 1425 889 649 505 471 307 283
275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4045 1537
Size: 275 2233 4090
Fragments arranged by size:
4090 2233 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2024 2937 4528 1353
Size: 671 913 1591 3423
Fragments arranged by size:
3423 1591 913 671
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2051 5185 5559 5705 387
Size: 1064 138 462 3134 374 146 1280
Fragments arranged by size:
3134 1280 1064 462 374 146 138
EcoICRI GAG'CTC
Cuts at: 3368 3368
Size: 6598
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2119 2292 2611 4661 5258 6045 6313
Size: 393 487 173 319 2050 597 787 268
Cuts at: 6313 1239
Size: 1524
Fragments arranged by size:
2050 1524 787 597 487 393 319 268
173
EcoO109I rG'GnC_Cy
Cuts at: 2017 2017
Size: 6598
EcoRI G'AATT_C
Cuts at: 3380 3907 4285 3380
Size: 527 378 5693
Fragments arranged by size:
5693 527 378
EcoRV GAT'ATC
Cuts at: 3046 3046
Size: 6598
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2551 3246 2551
Size: 695 5903
FspI TGC'GCA
Cuts at: 5104 5104
Size: 6598
HaeII r_GCGC'y
Cuts at: 1678 5745 1678
Size: 4067 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2438 2470 3380 3412 3437
Size: 32 135 32 866 32 910 32 25
Cuts at: 3437 3469 3569 3601 5239 5271 5313 5345 1373
Size: 32 100 32 1638 32 42 32 2626
Fragments arranged by size:
2626 1638 910 866 135 100 42 32
32 32 32 32 32 32 32 25
HincII GTy'rAC
Cuts at: 669 1657 1938 3100 3251 3942 4188 669
Size: 988 281 1162 151 691 246 3079
Fragments arranged by size:
3079 1162 988 691 281 246 151
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6598
HpaI GTT'AAC
Cuts at: 4188 4188
Size: 6598
KpnI G_GTAC'C
Cuts at: 3923 3923
Size: 6598
MfeI C'AATT_G
Cuts at: 4197 4197
Size: 6598
MluI A'CGCG_T
Cuts at: 6474 6474
Size: 6598
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2416 5712 5896 1305
Size: 1111 3296 184 2007
Fragments arranged by size:
3296 2007 1111 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2764 3235 10
Size: 54 1425 343 932 471 3373
Fragments arranged by size:
3373 1425 932 471 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2758 4556 4915 5074 518
Size: 648 1592 1798 359 159 2042
Fragments arranged by size:
2042 1798 1592 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2067 4014 4691 5839 6084 1606
Size: 461 1947 677 1148 245 2120
Fragments arranged by size:
2120 1947 1148 677 461 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6046
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6598
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6598
NotI GC'GGCC_GC
Cuts at: 4045 4045
Size: 6598
NspI r_CATG'y
Cuts at: 1132 1229 2569 3397 3620 3956 5501 1132
Size: 97 1340 828 223 336 1545 2229
Fragments arranged by size:
2229 1545 1340 828 336 223 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6598
PciI A'CATG_T
Cuts at: 3393 3616 5497 3393
Size: 223 1881 4494
Fragments arranged by size:
4494 1881 223
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6598
PmeI GTTT'AAAC
Cuts at: 3902 3902
Size: 6598
PmlI CAC'GTG
Cuts at: 2938 2938
Size: 6598
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2139 2171 4667 4699 6180 6212 2139
Size: 32 2496 32 1481 32 2525
Fragments arranged by size:
2525 2496 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6598
PsiI TTA'TAA
Cuts at: 4168 4168
Size: 6598
PspOMI G'GGCC_C
Cuts at: 2016 2016
Size: 6598
PstI C_TGCA'G
Cuts at: 830 3487 3950 830
Size: 2657 463 3478
Fragments arranged by size:
3478 2657 463
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6598
PvuII CAG'CTG
Cuts at: 2067 2067
Size: 6598
SacI G_AGCT'C
Cuts at: 3370 3370
Size: 6598
SalI G'TCGA_C
Cuts at: 1936 3940 1936
Size: 2004 4594
SapI GCTCTTCn'nnn_
Cuts at: 2937 2937
Size: 6598
SbfI CC_TGCA'GG
Cuts at: 3950 3950
Size: 6598
ScaI AGT'ACT
Cuts at: 4846 6558 4846
Size: 1712 4886
SfcI C'TryA_G
Cuts at: 826 1045 2328 3483 3946 5081 5762 5953 826
Size: 219 1283 1155 463 1135 681 191 1471
Fragments arranged by size:
1471 1283 1155 1135 681 463 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2112 2112
Size: 6598
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6598
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6598
SmaI CCC'GGG
Cuts at: 1872 3925 1872
Size: 2053 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4702 5603 5865 820
Size: 197 926 2759 901 262 1553
Fragments arranged by size:
2759 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4389 493
Size: 3896 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6598
SphI G_CATG'C
Cuts at: 1132 3956 1132
Size: 2824 3774
SspI AAT'ATT
Cuts at: 5 52 3646 4522 5
Size: 47 3594 876 2081
Fragments arranged by size:
3594 2081 876 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6598
StyI C'CwwG_G
Cuts at: 513 1065 2056 3472 3638 4037 513
Size: 552 991 1416 166 399 3074
Fragments arranged by size:
3074 1416 991 552 399 166
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2140 2929 3219 3389 3415 4625 4642
Size: 1255 560 789 290 170 26 1210 17
Cuts at: 4642 4795 4980 5425 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1210 789 560 445 290 185
170 153 26 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3658 4844 6556
Size: 274 80 33 51 1443 1681 1186 1712
Cuts at: 6556 96
Size: 138
Fragments arranged by size:
1712 1681 1443 1186 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6558 6590 6558
Size: 32 6566
Tth111I GACn'n_nGTC
Cuts at: 1316 3410 1316
Size: 2094 4504
XbaI T'CTAG_A
Cuts at: 2873 3934 2873
Size: 1061 5537
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2402 2457 2923 2402
Size: 55 466 6077
Fragments arranged by size:
6077 466 55
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6598
XmaI C'CCGG_G
Cuts at: 1870 3923 1870
Size: 2053 4545
XmnI GAAnn'nnTTC
Cuts at: 4727 4727
Size: 6598
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4406 276
Size: 53 83 186 936 2872 2468
Fragments arranged by size:
2872 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PpuMI PspXI PsrI RsrII SacII SanDI
SexAI SfoI SrfI SwaI