Restriction map for
pFN21AE5788
(Circular) (Six-base) MAPSORT of: pFN21AE5788.seq Check: 7256 from: 1 to: 7045
pFN21AE5788 7045 bp
With 182 enzymes: SgfI *
October 25, 2019 12:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2015 3388 4855 278
Size: 53 83 186 936 479 1373 1467 2468
Fragments arranged by size:
2468 1467 1373 936 479 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4388 1937
Size: 2451 4594
Acc65I G'GTAC_C
Cuts at: 3992 4366 3992
Size: 374 6671
AclI AA'CG_TT
Cuts at: 1563 5172 5545 1563
Size: 3609 373 3063
Fragments arranged by size:
3609 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2027 3306 3534 5108 6492 6760 1632
Size: 395 1279 228 1574 1384 268 1917
Fragments arranged by size:
1917 1574 1384 1279 395 268 228
AflII C'TTAA_G
Cuts at: 820 1017 3326 3860 820
Size: 197 2309 534 4005
Fragments arranged by size:
4005 2309 534 197
AflIII A'CryG_T
Cuts at: 1203 3243 4236 5944 6921 1203
Size: 2040 993 1708 977 1327
Fragments arranged by size:
2040 1708 1327 993 977
AgeI A'CCGG_T
Cuts at: 5876 5876
Size: 7045
AhdI GACnn_n'nnGTC
Cuts at: 3384 5774 3384
Size: 2390 4655
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7045
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7045
AlwNI CAG_nnn'CTG
Cuts at: 2993 4788 6360 2993
Size: 1795 1572 3678
Fragments arranged by size:
3678 1795 1572
ApaLI G'TGCA_C
Cuts at: 5102 6258 5102
Size: 1156 5889
ApoI r'AATT_y
Cuts at: 1493 2184 2592 3591 3683 4224 4354 4580 4732
Size: 691 408 999 92 541 130 226 152
Cuts at: 4732 6841 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 999 691 541 408 226 152
130 92
AseI AT'TA_AT
Cuts at: 160 1032 5599 160
Size: 872 4567 1606
Fragments arranged by size:
4567 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7045
AvaI C'yCGr_G
Cuts at: 1870 1943 4370 1870
Size: 73 2427 4545
Fragments arranged by size:
4545 2427 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3314 3347 3314
Size: 33 7012
BamHI G'GATC_C
Cuts at: 4375 4742 4375
Size: 367 6678
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3605 3992 4366 5821 618
Size: 325 217 627 1818 387 374 1455 1842
Fragments arranged by size:
1842 1818 1455 627 387 374 325 217
BanII G_rGCy'C
Cuts at: 1391 4364 1391
Size: 2973 4072
BbeI G_GCGC'C
Cuts at: 3609 3609
Size: 7045
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2058 2403 928
Size: 971 159 345 5570
Fragments arranged by size:
5570 971 345 159
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5236 5270 1903
Size: 34 3299 34 3678
Fragments arranged by size:
3678 3299 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4937 6147 4937
Size: 1210 5835
BclI T'GATC_A
Cuts at: 1548 2856 1548
Size: 1308 5737
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5656 136
Size: 107 122 71 631 605 3984 1525
Fragments arranged by size:
3984 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7040 7040
Size: 7045
BlpI GC'TnA_GC
Cuts at: 4200 4462 4200
Size: 262 6783
Bme1580I G_kGCm'C
Cuts at: 1790 5106 6262 1790
Size: 3316 1156 2573
Fragments arranged by size:
3316 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7045
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4776 5734 442
Size: 416 53 83 399 24 3359 958 1753
Fragments arranged by size:
3359 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7045
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3677 5705 1239
Size: 2438 2028 2579
Fragments arranged by size:
2579 2438 2028
Bpu10I CC'TnA_GC
Cuts at: 3219 3219
Size: 7045
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2234 3945 5170 6035 6333 2234
Size: 1711 1225 865 298 2946
Fragments arranged by size:
2946 1711 1225 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5708 882
Size: 617 4209 2219
Fragments arranged by size:
4209 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4836 493
Size: 711 3632 2702
Fragments arranged by size:
3632 2702 711
BsaBI GATnn'nnATC
Cuts at: 7039 7039
Size: 7045
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2012 3385 3606 4852
Size: 53 83 186 936 479 1373 221 1246
Cuts at: 4852 5234 5869 275
Size: 382 635 1451
Fragments arranged by size:
1451 1373 1246 936 635 479 382 221
186 83 53
BsaWI w'CCGG_w
Cuts at: 3670 5478 5876 6150 6297 6708 3670
Size: 1808 398 274 147 411 4007
Fragments arranged by size:
4007 1808 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6820
Fragments arranged by size:
6820 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7045
BseYI C'CCAG_C
Cuts at: 2176 6248 6744 2176
Size: 4072 496 2477
Fragments arranged by size:
4072 2477 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6472
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4495 5256 6284 1540
Size: 275 186 2494 761 1028 2301
Fragments arranged by size:
2494 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2740 3318 4293 5106 5191 6262 2740
Size: 578 975 813 85 1071 3523
Fragments arranged by size:
3523 1071 975 813 578 85
BsmI GAATG_Cn'
Cuts at: 2798 3604 4556 4649 2798
Size: 806 952 93 5194
Fragments arranged by size:
5194 952 806 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2740 3318 4293 4364 5106 5191 6262
Size: 399 950 578 975 71 742 85 1071
Cuts at: 6262 1391
Size: 2174
Fragments arranged by size:
2174 1071 975 950 742 578 399 85
71
BspEI T'CCGG_A
Cuts at: 6708 6708
Size: 7045
BspHI T'CATG_A
Cuts at: 4934 6664 4934
Size: 1730 5315
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4400 844
Size: 3556 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4932 1394
Size: 429 3109 3507
Fragments arranged by size:
3507 3109 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2487 5540 5714 65
Size: 1183 1239 3053 174 1396
Fragments arranged by size:
3053 1396 1239 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5689 5876 1700
Size: 110 3879 187 2869
Fragments arranged by size:
3879 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2683 3334 96
Size: 2587 651 3807
Fragments arranged by size:
3807 2587 651
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7045
BssSI C'ACGA_G
Cuts at: 2238 5105 6117 2238
Size: 2867 1012 3166
Fragments arranged by size:
3166 2867 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7045
BstBI TT'CG_AA
Cuts at: 2410 5849 2410
Size: 3439 3606
BstEII G'GTnAC_C
Cuts at: 1184 3787 1184
Size: 2603 4442
BstYI r'GATC_y
Cuts at: 1972 4375 4742 5127 5144 6596 6682 6694 7040
Size: 2403 367 385 17 1452 86 12 346
Cuts at: 7040 1972
Size: 1977
Fragments arranged by size:
2403 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2577 2577
Size: 7045
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5554
Fragments arranged by size:
5554 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6306
Fragments arranged by size:
6306 683 56
BtsI GCAGTG_nn'
Cuts at: 2126 4561 5355 5375 2126
Size: 2435 794 20 3796
Fragments arranged by size:
3796 2435 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7045
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4259 4294 529
Size: 35 3695 35 3280
Fragments arranged by size:
3695 3280 35 35
DraI TTT'AAA
Cuts at: 2199 2824 3154 4349 2199
Size: 625 330 1195 4895
Fragments arranged by size:
4895 1195 625 330
DraIII CAC_nnn'GTG
Cuts at: 2315 2315
Size: 7045
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3907 6052 809
Size: 507 2591 2145 1802
Fragments arranged by size:
2591 2145 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4492 5381 7010
Size: 54 1425 50 275 18 2662 889 1629
Cuts at: 7010 8
Size: 43
Fragments arranged by size:
2662 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4492 1537
Size: 275 2680 4090
Fragments arranged by size:
4090 2680 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2046 3282 4004 4104 4300 4975 1353
Size: 693 1236 722 100 196 675 3423
Fragments arranged by size:
3423 1236 722 693 675 196 100
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5632 6006 6152 387
Size: 1064 138 4043 374 146 1280
Fragments arranged by size:
4043 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2027 3306 3534 3677 5108 5705 6492
Size: 393 395 1279 228 143 1431 597 787
Cuts at: 6492 6760 1239
Size: 268 1524
Fragments arranged by size:
1524 1431 1279 787 597 395 393 268
228 143
EcoRI G'AATT_C
Cuts at: 3683 4354 4732 3683
Size: 671 378 5996
Fragments arranged by size:
5996 671 378
EcoRV GAT'ATC
Cuts at: 4321 4321
Size: 7045
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2057 2228 2638 3320 2057
Size: 171 410 682 5782
Fragments arranged by size:
5782 682 410 171
FspI TGC'GCA
Cuts at: 5551 5551
Size: 7045
HaeII r_GCGC'y
Cuts at: 1678 3609 6192 1678
Size: 1931 2583 2531
Fragments arranged by size:
2583 2531 1931
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2002 2034 3370 3402 3828
Size: 32 135 32 430 32 1336 32 426
Cuts at: 3828 3860 5686 5718 5760 5792 1373
Size: 32 1826 32 42 32 2626
Fragments arranged by size:
2626 1826 1336 430 426 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2342 2560 3967 4389 4635 669
Size: 988 281 404 218 1407 422 246 3079
Fragments arranged by size:
3079 1407 988 422 404 281 246 218
HindIII A'AGCT_T
Cuts at: 748 2087 2414 2514 3075 3302 3479 3528 3863
Size: 1339 327 100 561 227 177 49 335
Cuts at: 3863 748
Size: 3930
Fragments arranged by size:
3930 1339 561 335 327 227 177 100
49
HpaI GTT'AAC
Cuts at: 2560 4635 2560
Size: 2075 4970
KasI G'GCGC_C
Cuts at: 3605 3605
Size: 7045
KpnI G_GTAC'C
Cuts at: 3996 4370 3996
Size: 374 6671
MfeI C'AATT_G
Cuts at: 4644 4644
Size: 7045
MluI A'CGCG_T
Cuts at: 6921 6921
Size: 7045
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6159 6343 1305
Size: 4854 184 2007
Fragments arranged by size:
4854 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5223
Fragments arranged by size:
5223 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2256 4337 5003 5362 5521 518
Size: 648 1090 2081 666 359 159 2042
Fragments arranged by size:
2081 2042 1090 666 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2530 2990 3464 3572 4204 4461 5138 6286
Size: 924 460 474 108 632 257 677 1148
Cuts at: 6286 6531 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 924 677 632 474 460 257
245 108
NarI GG'CG_CC
Cuts at: 3606 3606
Size: 7045
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5554
Fragments arranged by size:
5554 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7045
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7045
NotI GC'GGCC_GC
Cuts at: 4492 4492
Size: 7045
NspI r_CATG'y
Cuts at: 1132 1229 2150 2498 3247 4240 4403 5948 1132
Size: 97 921 348 749 993 163 1545 2229
Fragments arranged by size:
2229 1545 993 921 749 348 163 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7045
PciI A'CATG_T
Cuts at: 3243 4236 5944 3243
Size: 993 1708 4344
Fragments arranged by size:
4344 1708 993
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7045
PmeI GTTT'AAAC
Cuts at: 4349 4349
Size: 7045
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5114 5146 6627 6659 5114
Size: 32 1481 32 5500
Fragments arranged by size:
5500 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7045
PsiI TTA'TAA
Cuts at: 3749 4615 3749
Size: 866 6179
PstI C_TGCA'G
Cuts at: 830 4209 4397 830
Size: 3379 188 3478
Fragments arranged by size:
3478 3379 188
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7045
PvuII CAG'CTG
Cuts at: 2530 2990 3572 4204 2530
Size: 460 582 632 5371
Fragments arranged by size:
5371 632 582 460
SalI G'TCGA_C
Cuts at: 1936 4387 1936
Size: 2451 4594
SbfI CC_TGCA'GG
Cuts at: 4397 4397
Size: 7045
ScaI AGT'ACT
Cuts at: 2244 5293 7005 2244
Size: 3049 1712 2284
Fragments arranged by size:
3049 2284 1712
SfcI C'TryA_G
Cuts at: 826 1045 4089 4205 4393 5528 6209 6400 826
Size: 219 3044 116 188 1135 681 191 1471
Fragments arranged by size:
3044 1471 1135 681 219 191 188 116
SfoI GGC'GCC
Cuts at: 3607 3607
Size: 7045
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7045
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7045
SmaI CCC'GGG
Cuts at: 1872 4372 1872
Size: 2500 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2213 3326 3860 3960 5149 6050
Size: 197 926 270 1113 534 100 1189 901
Cuts at: 6050 6312 820
Size: 262 1553
Fragments arranged by size:
1553 1189 1113 926 901 534 270 262
197 100
SnaBI TAC'GTA
Cuts at: 493 4836 493
Size: 4343 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7045
SphI G_CATG'C
Cuts at: 1132 2150 4403 1132
Size: 1018 2253 3774
Fragments arranged by size:
3774 2253 1018
SspI AAT'ATT
Cuts at: 5 52 4969 5
Size: 47 4917 2081
Fragments arranged by size:
4917 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7045
StyI C'CwwG_G
Cuts at: 513 1065 2004 2848 4484 513
Size: 552 939 844 1636 3074
Fragments arranged by size:
3074 1636 939 844 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3988 5072 5089 5242 5427 5872 325
Size: 1255 2408 1084 17 153 185 445 1498
Fragments arranged by size:
2408 1498 1255 1084 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2242 2683 3334
Size: 274 80 33 51 1443 265 441 651
Cuts at: 3334 5291 7003 96
Size: 1957 1712 138
Fragments arranged by size:
1957 1712 1443 651 441 274 265 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7005 7037 7005
Size: 32 7013
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7045
XbaI T'CTAG_A
Cuts at: 4381 4381
Size: 7045
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7045
XmaI C'CCGG_G
Cuts at: 1870 4370 1870
Size: 2500 4545
XmnI GAAnn'nnTTC
Cuts at: 2208 3078 5174 2208
Size: 870 2096 4079
Fragments arranged by size:
4079 2096 870
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2013 3386 4853 276
Size: 53 83 186 936 479 1373 1467 2468
Fragments arranged by size:
2468 1467 1373 936 479 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI EcoO109I
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SrfI SwaI XcmI