Restriction map for
pFN21AE5787
(Circular) (Six-base) MAPSORT of: pFN21AE5787.seq Check: 3771 from: 1 to: 6958
pFN21AE5787 6958 bp
With 182 enzymes: SgfI *
December 8, 2014 13:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4197 4768 278
Size: 53 83 186 936 2661 571 2468
Fragments arranged by size:
2661 2468 936 571 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3450 4301 1937
Size: 1513 851 4594
Fragments arranged by size:
4594 1513 851
Acc65I G'GTAC_C
Cuts at: 4279 4279
Size: 6958
AclI AA'CG_TT
Cuts at: 1563 2502 4257 5085 5458 1563
Size: 939 1755 828 373 3063
Fragments arranged by size:
3063 1755 939 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2988 5021 6405 6673 1632
Size: 1356 2033 1384 268 1917
Fragments arranged by size:
2033 1917 1384 1356 268
AfeI AGC'GCT
Cuts at: 2495 2495
Size: 6958
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6761
AflIII A'CryG_T
Cuts at: 1203 5857 6834 1203
Size: 4654 977 1327
Fragments arranged by size:
4654 1327 977
AgeI A'CCGG_T
Cuts at: 5789 5789
Size: 6958
AhdI GACnn_n'nnGTC
Cuts at: 5687 5687
Size: 6958
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6958
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6958
AlwNI CAG_nnn'CTG
Cuts at: 3526 4701 6273 3526
Size: 1175 1572 4211
Fragments arranged by size:
4211 1572 1175
ApaI G_GGCC'C
Cuts at: 2056 2407 2563 3797 2056
Size: 351 156 1234 5217
Fragments arranged by size:
5217 1234 351 156
ApaLI G'TGCA_C
Cuts at: 5015 6171 5015
Size: 1156 5802
ApoI r'AATT_y
Cuts at: 1493 3398 3707 3980 4267 4493 4645 6754 1493
Size: 1905 309 273 287 226 152 2109 1697
Fragments arranged by size:
2109 1905 1697 309 287 273 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4200 5512 160
Size: 872 3168 1312 1606
Fragments arranged by size:
3168 1606 1312 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6958
AvaI C'yCGr_G
Cuts at: 1870 1943 4283 1870
Size: 73 2340 4545
Fragments arranged by size:
4545 2340 73
AvrII C'CTAG_G
Cuts at: 2065 2065
Size: 6958
BamHI G'GATC_C
Cuts at: 4288 4655 4288
Size: 367 6591
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4279 5734 618
Size: 325 217 627 2492 1455 1842
Fragments arranged by size:
2492 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2056 2407 2546 2563 3797 3825 4277 1391
Size: 665 351 139 17 1234 28 452 4072
Fragments arranged by size:
4072 1234 665 452 351 139 28 17
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2103 4197 928
Size: 971 204 2094 3689
Fragments arranged by size:
3689 2094 971 204
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5149 5183 1903
Size: 34 3212 34 3678
Fragments arranged by size:
3678 3212 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4850 6060 4850
Size: 1210 5748
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6958
BfrBI ATG'CAT
Cuts at: 2233 3208 2233
Size: 975 5983
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2033 5569 136
Size: 107 122 71 631 605 361 3536 1525
Fragments arranged by size:
3536 1525 631 605 361 122 107 71
BglII A'GATC_T
Cuts at: 2650 6953 2650
Size: 4303 2655
BlpI GC'TnA_GC
Cuts at: 4375 4375
Size: 6958
Bme1580I G_kGCm'C
Cuts at: 1790 2056 2407 2563 3797 5019 6175 1790
Size: 266 351 156 1234 1222 1156 2573
Fragments arranged by size:
2573 1234 1222 1156 351 266 156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6958
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4041 4689 5647
Size: 416 53 83 399 24 2624 648 958
Cuts at: 5647 442
Size: 1753
Fragments arranged by size:
2624 1753 958 648 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2849 1056
Size: 1793 5165
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3022 3022
Size: 6958
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3022 3104 3692 5618 1239
Size: 1783 82 588 1926 2579
Fragments arranged by size:
2579 1926 1783 588 82
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5083 5948 6246 5083
Size: 865 298 5795
Fragments arranged by size:
5795 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3578 5621 882
Size: 617 2079 2043 2219
Fragments arranged by size:
2219 2079 2043 617
BsaAI yAC'GTr
Cuts at: 493 1204 4749 493
Size: 711 3545 2702
Fragments arranged by size:
3545 2702 711
BsaBI GATnn'nnATC
Cuts at: 6952 6952
Size: 6958
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4015 4194 4765 5147
Size: 53 83 186 936 2482 179 571 382
Cuts at: 5147 5782 275
Size: 635 1451
Fragments arranged by size:
2482 1451 936 635 571 382 186 179
83 53
BsaWI w'CCGG_w
Cuts at: 3825 5391 5789 6063 6210 6621 3825
Size: 1566 398 274 147 411 4162
Fragments arranged by size:
4162 1566 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6733
Fragments arranged by size:
6733 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6958
BseYI C'CCAG_C
Cuts at: 2551 6161 6657 2551
Size: 3610 496 2852
Fragments arranged by size:
3610 2852 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2445 3660 1163
Size: 573 709 1215 4461
Fragments arranged by size:
4461 1215 709 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4408 5169 6197 1540
Size: 275 186 2407 761 1028 2301
Fragments arranged by size:
2407 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3850 3904 5019 5104 6175 3850
Size: 54 1115 85 1071 4633
Fragments arranged by size:
4633 1115 1071 85 54
BsmI GAATG_Cn'
Cuts at: 4469 4562 4469
Size: 93 6865
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2056 2407 2546 2563 3797 3825 3850
Size: 399 266 351 139 17 1234 28 25
Cuts at: 3850 3904 4277 5019 5104 6175 1391
Size: 54 373 742 85 1071 2174
Fragments arranged by size:
2174 1234 1071 742 399 373 351 266
139 85 54 28 25 17
BspEI T'CCGG_A
Cuts at: 6621 6621
Size: 6958
BspHI T'CATG_A
Cuts at: 4847 6577 4847
Size: 1730 5228
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2074 3408 4149 4313 844
Size: 1230 1334 741 164 3489
Fragments arranged by size:
3489 1334 1230 741 164
BsrBI CCG'CTC
Cuts at: 1394 1823 4845 1394
Size: 429 3022 3507
Fragments arranged by size:
3507 3022 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3372 5453 5627 65
Size: 1183 2124 2081 174 1396
Fragments arranged by size:
2124 2081 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2751 5602 5789 1700
Size: 110 941 2851 187 2869
Fragments arranged by size:
2869 2851 941 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6958
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6958
BssSI C'ACGA_G
Cuts at: 5018 6030 5018
Size: 1012 5946
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3751 794
Size: 2957 4001
BstBI TT'CG_AA
Cuts at: 2854 5762 2854
Size: 2908 4050
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6958
BstXI CCAn_nnnn'nTGG
Cuts at: 2623 2623
Size: 6958
BstYI r'GATC_y
Cuts at: 1972 2650 3433 4288 4655 5040 5057 6509 6595
Size: 678 783 855 367 385 17 1452 86
Cuts at: 6595 6607 6953 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 855 783 678 385 367 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 3451 3451
Size: 6958
BtgI C'CryG_G
Cuts at: 513 1065 2277 2616 4018 513
Size: 552 1212 339 1402 3453
Fragments arranged by size:
3453 1402 1212 552 339
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6219
Fragments arranged by size:
6219 683 56
BtsI GCAGTG_nn'
Cuts at: 4474 5268 5288 4474
Size: 794 20 6144
Fragments arranged by size:
6144 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6958
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2376 2411 529
Size: 35 1812 35 5076
Fragments arranged by size:
5076 1812 35 35
DraI TTT'AAA
Cuts at: 2638 2841 3079 3445 4262 2638
Size: 203 238 366 817 5334
Fragments arranged by size:
5334 817 366 238 203
DraIII CAC_nnn'GTG
Cuts at: 2334 2334
Size: 6958
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5965 809
Size: 507 4649 1802
Fragments arranged by size:
4649 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2739 4405 5294
Size: 54 1425 50 275 18 909 1666 889
Cuts at: 5294 6923 8
Size: 1629 43
Fragments arranged by size:
1666 1629 1425 909 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4405 1537
Size: 275 2593 4090
Fragments arranged by size:
4090 2593 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2851 4888 1353
Size: 1498 2037 3423
Fragments arranged by size:
3423 2037 1498
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5545 5919 6065 387
Size: 1064 138 3956 374 146 1280
Fragments arranged by size:
3956 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2988 3022 3104 3692 5021 5618 6405
Size: 393 1356 34 82 588 1329 597 787
Cuts at: 6405 6673 1239
Size: 268 1524
Fragments arranged by size:
1524 1356 1329 787 597 588 393 268
82 34
EcoO109I rG'GnC_Cy
Cuts at: 2052 2053 2403 2404 2559 2560 3793 3794 2052
Size: 1 350 1 155 1 1233 1 5216
Fragments arranged by size:
5216 1233 350 155 1 1 1 1
EcoRI G'AATT_C
Cuts at: 4267 4645 4267
Size: 378 6580
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3920 3920
Size: 6958
FspI TGC'GCA
Cuts at: 5464 5464
Size: 6958
HaeII r_GCGC'y
Cuts at: 1678 2497 2904 6105 1678
Size: 819 407 3201 2531
Fragments arranged by size:
3201 2531 819 407
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2464 2496 5599 5631 5673
Size: 32 135 32 892 32 3103 32 42
Cuts at: 5673 5705 1373
Size: 32 2626
Fragments arranged by size:
3103 2626 892 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4226 4302 4548 669
Size: 988 281 2288 76 246 3079
Fragments arranged by size:
3079 2288 988 281 246 76
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6958
HpaI GTT'AAC
Cuts at: 4548 4548
Size: 6958
KpnI G_GTAC'C
Cuts at: 4283 4283
Size: 6958
MfeI C'AATT_G
Cuts at: 4557 4557
Size: 6958
MluI A'CGCG_T
Cuts at: 6834 6834
Size: 6958
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2397 2428 2571 6072 6256 1305
Size: 1092 31 143 3501 184 2007
Fragments arranged by size:
3501 2007 1092 184 143 31
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5136
Fragments arranged by size:
5136 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4916 5275 5434 518
Size: 648 3750 359 159 2042
Fragments arranged by size:
3750 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2723 3315 4374 5051 6199 6444 1606
Size: 1117 592 1059 677 1148 245 2120
Fragments arranged by size:
2120 1148 1117 1059 677 592 245
NcoI C'CATG_G
Cuts at: 513 1065 2277 2616 4018 513
Size: 552 1212 339 1402 3453
Fragments arranged by size:
3453 1402 1212 552 339
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6958
NheI G'CTAG_C
Cuts at: 1052 2845 1052
Size: 1793 5165
NotI GC'GGCC_GC
Cuts at: 4405 4405
Size: 6958
NsiI A_TGCA'T
Cuts at: 2235 3210 2235
Size: 975 5983
NspI r_CATG'y
Cuts at: 1132 1229 2378 3208 3212 4316 5861 1132
Size: 97 1149 830 4 1104 1545 2229
Fragments arranged by size:
2229 1545 1149 1104 830 97 4
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6958
PciI A'CATG_T
Cuts at: 5857 5857
Size: 6958
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6958
PfoI T'CCnGG_A
Cuts at: 3669 3669
Size: 6958
PmeI GTTT'AAAC
Cuts at: 4262 4262
Size: 6958
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5027 5059 6540 6572 5027
Size: 32 1481 32 5413
Fragments arranged by size:
5413 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2691 1613
Size: 1078 5880
PsiI TTA'TAA
Cuts at: 4528 4528
Size: 6958
PspOMI G'GGCC_C
Cuts at: 2052 2403 2559 3793 2052
Size: 351 156 1234 5217
Fragments arranged by size:
5217 1234 351 156
PstI C_TGCA'G
Cuts at: 830 2026 2568 4310 830
Size: 1196 542 1742 3478
Fragments arranged by size:
3478 1742 1196 542
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6958
PvuII CAG'CTG
Cuts at: 2723 2723
Size: 6958
SalI G'TCGA_C
Cuts at: 1936 4300 1936
Size: 2364 4594
SapI GCTCTTCn'nnn_
Cuts at: 2851 2851
Size: 6958
SbfI CC_TGCA'GG
Cuts at: 4310 4310
Size: 6958
ScaI AGT'ACT
Cuts at: 5206 6918 5206
Size: 1712 5246
SfcI C'TryA_G
Cuts at: 826 1045 2022 2564 3759 4218 4306 5441 6122
Size: 219 977 542 1195 459 88 1135 681
Cuts at: 6122 6313 826
Size: 191 1471
Fragments arranged by size:
1471 1195 1135 977 681 542 459 219
191 88
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6958
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6958
SmaI CCC'GGG
Cuts at: 1872 4285 1872
Size: 2413 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5062 5963 6225 820
Size: 197 926 3119 901 262 1553
Fragments arranged by size:
3119 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4749 493
Size: 4256 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6958
SphI G_CATG'C
Cuts at: 1132 3208 3212 4316 1132
Size: 2076 4 1104 3774
Fragments arranged by size:
3774 2076 1104 4
SspI AAT'ATT
Cuts at: 5 52 2149 2350 3736 4882 5
Size: 47 2097 201 1386 1146 2081
Fragments arranged by size:
2097 2081 1386 1146 201 47
StuI AGG'CCT
Cuts at: 1517 2099 3069 1517
Size: 582 970 5406
Fragments arranged by size:
5406 970 582
StyI C'CwwG_G
Cuts at: 513 1065 2065 2277 2616 3915 4018 4397 513
Size: 552 1000 212 339 1299 103 379 3074
Fragments arranged by size:
3074 1299 1000 552 379 339 212 103
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2550 3072 4985 5002 5155 5340 5785
Size: 1255 970 522 1913 17 153 185 445
Cuts at: 5785 325
Size: 1498
Fragments arranged by size:
1913 1498 1255 970 522 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5204 6916 96
Size: 274 80 33 51 1443 3227 1712 138
Fragments arranged by size:
3227 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6918 6950 6918
Size: 32 6926
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6958
XbaI T'CTAG_A
Cuts at: 4294 4294
Size: 6958
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 6958
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6958
XmaI C'CCGG_G
Cuts at: 1870 4283 1870
Size: 2413 4545
XmnI GAAnn'nnTTC
Cuts at: 5087 5087
Size: 6958
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4195 4766 276
Size: 53 83 186 936 2661 571 2468
Fragments arranged by size:
2661 2468 936 571 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI BaeI BbeI BbvCI Bpu10I BsiWI
BsmBI Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspXI
PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI